Restriction map for pF1KA1180
 (Linear) (Six-base) MAPSORT of: KIAA1180.seq  Check: 2511  from: 1  to: 1056



KIAA1180.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:22  ..

Acc65I G'GTAC_C

Cuts at:      0     248    1056 
   Size:        248     808
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     492     793     848    1056 
   Size:        492     301      55     208

  Fragments arranged by size: 

                 492     301     208      55
 
AflIII A'CryG_T

Cuts at:      0     608    1056 
   Size:        608     448
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     293    1056 
   Size:        293     763
 
AlwNI CAG_nnn'CTG

Cuts at:      0     787    1056 
   Size:        787     269
 
BanI G'GyrC_C

Cuts at:      0     248     340     654    1056 
   Size:        248      92     314     402

  Fragments arranged by size: 

                 402     314     248      92
 
BanII G_rGCy'C

Cuts at:      0      73    1056 
   Size:         73     983
 
BbeI G_GCGC'C

Cuts at:      0     344    1056 
   Size:        344     712
 
BbvCI CC'TCA_GC

Cuts at:      0     716     909    1056 
   Size:        716     193     147

  Fragments arranged by size: 

                 716     193     147
 
Bme1580I G_kGCm'C

Cuts at:      0     659     908    1056 
   Size:        659     249     148

  Fragments arranged by size: 

                 659     249     148
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     453    1056 
   Size:        453     603
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     756    1056 
   Size:        756     300
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     809    1056 
   Size:        809     247
 
Bpu10I CC'TnA_GC

Cuts at:      0     716     909    1056 
   Size:        716     193     147

  Fragments arranged by size: 

                 716     193     147
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     620    1056 
   Size:        620     436
 
BsaAI yAC'GTr

Cuts at:      0     201    1056 
   Size:        201     855
 
BsaBI GATnn'nnATC

Cuts at:      0     328    1056 
   Size:        328     728
 
BsaHI Gr'CG_yC

Cuts at:      0     229     341    1056 
   Size:        229     112     715

  Fragments arranged by size: 

                 715     229     112
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     338     368     887     917    1056 
   Size:        338      30     519      30     139

  Fragments arranged by size: 

                 519     338     139      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     532     911    1056 
   Size:        532     379     145

  Fragments arranged by size: 

                 532     379     145
 
BseYI C'CCAG_C

Cuts at:      0      11      90     289    1020    1056 
   Size:         11      79     199     731      36

  Fragments arranged by size: 

                 731     199      79      36      11
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319     565     978    1056 
   Size:        319     246     413      78

  Fragments arranged by size: 

                 413     319     246      78
 
BsiHKAI G_wGCw'C

Cuts at:      0     497    1056 
   Size:        497     559
 
Bsp1286I G_dGCh'C

Cuts at:      0      73     497     659     908    1056 
   Size:         73     424     162     249     148

  Fragments arranged by size: 

                 424     249     162     148      73
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     599    1056 
   Size:        599     457
 
BsrDI GCAATG_nn'

Cuts at:      0     863    1056 
   Size:        863     193
 
BsrFI r'CCGG_y

Cuts at:      0     554     641    1056 
   Size:        554      87     415

  Fragments arranged by size: 

                 554     415      87
 
BsrGI T'GTAC_A

Cuts at:      0     611    1056 
   Size:        611     445
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     969    1056 
   Size:        969      87
 
Bsu36I CC'TnA_GG

Cuts at:      0     242    1056 
   Size:        242     814
 
BtgI C'CryG_G

Cuts at:      0     263    1040    1056 
   Size:        263     777      16

  Fragments arranged by size: 

                 777     263      16
 
BtsI GCAGTG_nn'

Cuts at:      0     908     941    1056 
   Size:        908      33     115

  Fragments arranged by size: 

                 908     115      33
 
DraIII CAC_nnn'GTG

Cuts at:      0     189    1056 
   Size:        189     867
 
EaeI y'GGCC_r

Cuts at:      0     359    1056 
   Size:        359     697
 
EarI CTCTTCn'nnn_

Cuts at:      0     511     604    1056 
   Size:        511      93     452

  Fragments arranged by size: 

                 511     452      93
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     492     793     809     848    1056 
   Size:        492     301      16      39     208

  Fragments arranged by size: 

                 492     301     208      39      16
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     391    1056 
   Size:        391     665
 
HaeII r_GCGC'y

Cuts at:      0     344    1056 
   Size:        344     712
 
HincII GTy'rAC

Cuts at:      0    1032    1056 
   Size:       1032      24
 
KasI G'GCGC_C

Cuts at:      0     340    1056 
   Size:        340     716
 
KpnI G_GTAC'C

Cuts at:      0     252    1056 
   Size:        252     804
 
MscI TGG'CCA

Cuts at:      0     361    1056 
   Size:        361     695
 
MslI CAynn'nnrTG

Cuts at:      0     203     334    1045    1056 
   Size:        203     131     711      11

  Fragments arranged by size: 

                 711     203     131      11
 
MspA1I CmG'CkG

Cuts at:      0     273     677     715    1056 
   Size:        273     404      38     341

  Fragments arranged by size: 

                 404     341     273      38
 
NarI GG'CG_CC

Cuts at:      0     341    1056 
   Size:        341     715
 
NcoI C'CATG_G

Cuts at:      0     263    1040    1056 
   Size:        263     777      16

  Fragments arranged by size: 

                 777     263      16
 
NspI r_CATG'y

Cuts at:      0      58     168     612    1056 
   Size:         58     110     444     444

  Fragments arranged by size: 

                 444     444     110      58
 
PciI A'CATG_T

Cuts at:      0     608    1056 
   Size:        608     448
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     114    1041    1056 
   Size:        114     927      15

  Fragments arranged by size: 

                 927     114      15
 
PmlI CAC'GTG

Cuts at:      0     201    1056 
   Size:        201     855
 
PstI C_TGCA'G

Cuts at:      0     596    1056 
   Size:        596     460
 
PvuII CAG'CTG

Cuts at:      0     273    1056 
   Size:        273     783
 
SapI GCTCTTCn'nnn_

Cuts at:      0     604    1056 
   Size:        604     452
 
SexAI A'CCwGG_T

Cuts at:      0     386    1056 
   Size:        386     670
 
SfcI C'TryA_G

Cuts at:      0     592    1056 
   Size:        592     464
 
SfoI GGC'GCC

Cuts at:      0     342    1056 
   Size:        342     714
 
StuI AGG'CCT

Cuts at:      0     991    1056 
   Size:        991      65
 
StyI C'CwwG_G

Cuts at:      0      77     263    1040    1056 
   Size:         77     186     777      16

  Fragments arranged by size: 

                 777     186      77      16
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     896    1012    1056 
   Size:        896     116      44

  Fragments arranged by size: 

                 896     116      44
 
TatI w'GTAC_w

Cuts at:      0     611    1056 
   Size:        611     445
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     206     238     338     362     370     394     887     919 
   Size:        206      32     100      24       8      24     493      32

Cuts at:    919    1056 
   Size:        137

  Fragments arranged by size: 

                 493     206     137     100      32      32      24      24
                   8
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     186     270    1056 
   Size:        186      84     786

  Fragments arranged by size: 

                 786     186      84
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AlfI    AlwNI     BanI    BanII     BbeI
    BbvCI Bme1580I     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspMI    BsrDI    BsrFI    BsrGI    BstXI   Bsu36I     BtgI     BtsI
   DraIII     EaeI     EarI  Eco57MI    EcoNI    HaeII   HincII     KasI
     KpnI     MscI     MslI   MspA1I     NarI     NcoI     NspI     PciI
    PflMI     PmlI     PstI    PvuII     SapI    SexAI     SfcI     SfoI
     StuI     StyI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbsI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI    BpuEI    BsaWI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I    BtgZI     ClaI
    CspCI     DraI     DrdI     EagI     EciI  EcoICRI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I  HindIII     HpaI
     MfeI     MluI     MmeI     NaeI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmeI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI     SfiI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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