Restriction map for
pF1KA1122
(Circular) (Six-base) MAPSORT of: pF1KA1122.seq Check: 3782 from: 1 to: 6197
pF1KA1122 6197 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 700 700
Size: 6197
AccI GT'mk_AC
Cuts at: 3235 3235
Size: 6197
Acc65I G'GTAC_C
Cuts at: 2801 3213 2801
Size: 412 5785
AclI AA'CG_TT
Cuts at: 6037 6037
Size: 6197
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 248 263 509 577 731 2026 2915 4022 4454
Size: 15 246 68 154 1295 889 1107 432
Cuts at: 4454 5201 5469 248
Size: 747 268 976
Fragments arranged by size:
1295 1107 976 889 747 432 268 246
154 68 15
AflII C'TTAA_G
Cuts at: 640 640
Size: 6197
AflIII A'CryG_T
Cuts at: 2138 2604 3549 4653 5630 2138
Size: 466 945 1104 977 2705
Fragments arranged by size:
2705 1104 977 945 466
AgeI A'CCGG_T
Cuts at: 4585 4585
Size: 6197
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1372 1404 1372
Size: 32 6165
AlwNI CAG_nnn'CTG
Cuts at: 1198 3502 5069 1198
Size: 2304 1567 2326
Fragments arranged by size:
2326 2304 1567
ApaI G_GGCC'C
Cuts at: 722 722
Size: 6197
ApaLI G'TGCA_C
Cuts at: 4967 4967
Size: 6197
ApoI r'AATT_y
Cuts at: 769 892 1099 1271 2121 2677 3201 5550 5806
Size: 123 207 172 850 556 524 2349 256
Cuts at: 5806 769
Size: 1160
Fragments arranged by size:
2349 1160 850 556 524 256 207 172
123
AseI AT'TA_AT
Cuts at: 20 2048 2633 20
Size: 2028 585 3584
Fragments arranged by size:
3584 2028 585
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6197
AvaI C'yCGr_G
Cuts at: 3217 3217
Size: 6197
AvrII C'CTAG_G
Cuts at: 1683 1683
Size: 6197
BamHI G'GATC_C
Cuts at: 3222 3222
Size: 6197
BanI G'GyrC_C
Cuts at: 1808 2801 3213 3627 3875 3910 1808
Size: 993 412 414 248 35 4095
Fragments arranged by size:
4095 993 414 412 248 35
BanII G_rGCy'C
Cuts at: 722 2020 3211 4241 722
Size: 1298 1191 1030 2678
Fragments arranged by size:
2678 1298 1191 1030
BbeI G_GCGC'C
Cuts at: 3631 3879 3631
Size: 248 5949
BbsI GAAGACnn'nnnn_
Cuts at: 383 560 578 995 1181 1502 383
Size: 177 18 417 186 321 5078
Fragments arranged by size:
5078 417 321 186 177 18
BciVI GTATCCnnnnn_n'
Cuts at: 4095 4856 6197 4095
Size: 761 1341 4095
Fragments arranged by size:
4095 1341 761
BclI T'GATC_A
Cuts at: 780 3717 780
Size: 2937 3260
BfrBI ATG'CAT
Cuts at: 2696 2696
Size: 6197
BglII A'GATC_T
Cuts at: 3712 5749 3712
Size: 2037 4160
BlpI GC'TnA_GC
Cuts at: 3309 3309
Size: 6197
Bme1580I G_kGCm'C
Cuts at: 722 1813 3822 3915 4971 722
Size: 1091 2009 93 1056 1948
Fragments arranged by size:
2009 1948 1091 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 3409 3490 3825 5969 3409
Size: 81 335 2144 3637
Fragments arranged by size:
3637 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 946 4564 946
Size: 3618 2579
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 305 305
Size: 6197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 1582 1829 2275 2488 3373 4252 4744 5042
Size: 666 247 446 213 885 879 492 298
Cuts at: 5042 916
Size: 2071
Fragments arranged by size:
2071 885 879 666 492 446 298 247
213
BsaI GGTCTCn'nnnn_
Cuts at: 5901 5901
Size: 6197
BsaAI yAC'GTr
Cuts at: 526 3550 526
Size: 3024 3173
BsaBI GATnn'nnATC
Cuts at: 6 3711 5748 6
Size: 3705 2037 455
Fragments arranged by size:
3705 2037 455
BsaHI Gr'CG_yC
Cuts at: 3628 3876 4578 6074 3628
Size: 248 702 1496 3751
Fragments arranged by size:
3751 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3386 3907 4585 4859 5006 5417 3386
Size: 521 678 274 147 411 4166
Fragments arranged by size:
4166 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1372 1402 1372
Size: 30 6167
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 247 247
Size: 6197
BseYI C'CCAG_C
Cuts at: 191 3622 4380 4957 5453 191
Size: 3431 758 577 496 935
Fragments arranged by size:
3431 935 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3739 3785 4993 104
Size: 3635 46 1208 1308
Fragments arranged by size:
3635 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3211 3989 4179 4971 3211
Size: 778 190 792 4437
Fragments arranged by size:
4437 792 778 190
BsmI GAATG_Cn'
Cuts at: 243 243
Size: 6197
Bsp1286I G_dGCh'C
Cuts at: 722 1813 2020 3211 3822 3915 3989 4179 4241
Size: 1091 207 1191 611 93 74 190 62
Cuts at: 4241 4971 722
Size: 730 1948
Fragments arranged by size:
1948 1191 1091 730 611 207 190 93
74 62
BspEI T'CCGG_A
Cuts at: 5417 5417
Size: 6197
BspHI T'CATG_A
Cuts at: 2435 5373 2435
Size: 2938 3259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 700 3247 3461 3763 4144 700
Size: 2547 214 302 381 2753
Fragments arranged by size:
2753 2547 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4489 4543 6027 6192 50
Size: 4439 54 1484 165 55
Fragments arranged by size:
4439 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1703 2339 2436 4109 1703
Size: 636 97 1673 3791
Fragments arranged by size:
3791 1673 636 97
BsrFI r'CCGG_y
Cuts at: 4195 4376 4585 4195
Size: 181 209 5807
Fragments arranged by size:
5807 209 181
BsrGI T'GTAC_A
Cuts at: 950 2085 950
Size: 1135 5062
BssSI C'ACGA_G
Cuts at: 4468 4826 4468
Size: 358 5839
BstAPI GCAn_nnn'nTGC
Cuts at: 682 3574 682
Size: 2892 3305
BstBI TT'CG_AA
Cuts at: 2597 4558 2597
Size: 1961 4236
BstXI CCAn_nnnn'nTGG
Cuts at: 2760 2760
Size: 6197
BstYI r'GATC_y
Cuts at: 1 3222 3689 3712 4047 4293 5305 5391 5403
Size: 3221 467 23 335 246 1012 86 12
Cuts at: 5403 5749 1
Size: 346 449
Fragments arranged by size:
3221 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 518 518
Size: 6197
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2461 4147 4328 4491 2461
Size: 1686 181 163 4167
Fragments arranged by size:
4167 1686 181 163
BtsI GCAGTG_nn'
Cuts at: 3440 3440
Size: 6197
DraI TTT'AAA
Cuts at: 3196 3196
Size: 6197
DraIII CAC_nnn'GTG
Cuts at: 1018 2545 1018
Size: 1527 4670
DrdI GACnn_nn'nnGTC
Cuts at: 3903 4761 3903
Size: 858 5339
EaeI y'GGCC_r
Cuts at: 2456 3782 3956 4347 4374 5719 2456
Size: 1326 174 391 27 1345 2934
Fragments arranged by size:
2934 1345 1326 391 174 27
EagI C'GGCC_G
Cuts at: 3782 3782
Size: 6197
EarI CTCTTCn'nnn_
Cuts at: 224 491 2265 2276 4220 4430 224
Size: 267 1774 11 1944 210 1991
Fragments arranged by size:
1991 1944 1774 267 210 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4405 4715 4861 6007 4405
Size: 310 146 1146 4595
Fragments arranged by size:
4595 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3209 3209
Size: 6197
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 248 263 305 509 577 731 2026 2915 4022
Size: 15 42 204 68 154 1295 889 1107
Cuts at: 4022 4454 5201 5469 248
Size: 432 747 268 976
Fragments arranged by size:
1295 1107 976 889 747 432 268 204
154 68 42 15
EcoNI CCTnn'n_nnAGG
Cuts at: 1726 1726
Size: 6197
EcoO109I rG'GnC_Cy
Cuts at: 1824 3336 1824
Size: 1512 4685
EcoRI G'AATT_C
Cuts at: 3201 3201
Size: 6197
EcoRV GAT'ATC
Cuts at: 2766 2877 2766
Size: 111 6086
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4216 4216
Size: 6197
FspI TGC'GCA
Cuts at: 3978 3978
Size: 6197
HaeII r_GCGC'y
Cuts at: 169 3631 3879 4901 5880 169
Size: 3462 248 1022 979 486
Fragments arranged by size:
3462 1022 979 486 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 484 516 769 801 4039 4069 4071 4101 484
Size: 32 253 32 3238 30 2 30 2580
Fragments arranged by size:
3238 2580 253 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3236 3236
Size: 6197
HindIII A'AGCT_T
Cuts at: 959 959
Size: 6197
KasI G'GCGC_C
Cuts at: 3627 3875 3627
Size: 248 5949
KpnI G_GTAC'C
Cuts at: 2805 3217 2805
Size: 412 5785
MfeI C'AATT_G
Cuts at: 677 1298 677
Size: 621 5576
MluI A'CGCG_T
Cuts at: 5630 5630
Size: 6197
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2318 4868 5052 2318
Size: 2550 184 3463
Fragments arranged by size:
3463 2550 184
MscI TGG'CCA
Cuts at: 2458 3958 2458
Size: 1500 4697
MslI CAynn'nnrTG
Cuts at: 1396 1646 2422 4186 4313 1396
Size: 250 776 1764 127 3280
Fragments arranged by size:
3280 1764 776 250 127
MspA1I CmG'CkG
Cuts at: 2494 3308 3622 3982 4995 5240 2494
Size: 814 314 360 1013 245 3451
Fragments arranged by size:
3451 1013 814 360 314 245
NaeI GCC'GGC
Cuts at: 4378 4378
Size: 6197
NarI GG'CG_CC
Cuts at: 3628 3876 3628
Size: 248 5949
NcoI C'CATG_G
Cuts at: 518 518
Size: 6197
NdeI CA'TA_TG
Cuts at: 756 2000 756
Size: 1244 4953
NgoMIV G'CCGG_C
Cuts at: 4376 4376
Size: 6197
NsiI A_TGCA'T
Cuts at: 2698 2698
Size: 6197
NspI r_CATG'y
Cuts at: 2142 2239 2608 3250 3750 4657 2142
Size: 97 369 642 500 907 3682
Fragments arranged by size:
3682 907 642 500 369 97
PciI A'CATG_T
Cuts at: 2138 2604 4653 2138
Size: 466 2049 3682
Fragments arranged by size:
3682 2049 466
PmeI GTTT'AAAC
Cuts at: 3196 3196
Size: 6197
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1372 1404 5336 5368 1372
Size: 32 3932 32 2201
Fragments arranged by size:
3932 2201 32 32
PpuMI rG'GwC_Cy
Cuts at: 1824 1824
Size: 6197
PshAI GACnn'nnGTC
Cuts at: 1949 1949
Size: 6197
PsiI TTA'TAA
Cuts at: 1474 2251 2975 3037 1474
Size: 777 724 62 4634
Fragments arranged by size:
4634 777 724 62
PspOMI G'GGCC_C
Cuts at: 718 718
Size: 6197
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2591 2623 2591
Size: 32 6165
PstI C_TGCA'G
Cuts at: 837 3244 3929 837
Size: 2407 685 3105
Fragments arranged by size:
3105 2407 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6197
PvuII CAG'CTG
Cuts at: 2494 3622 3982 2494
Size: 1128 360 4709
Fragments arranged by size:
4709 1128 360
RsrII CG'GwC_CG
Cuts at: 4392 4392
Size: 6197
SacI G_AGCT'C
Cuts at: 3211 3211
Size: 6197
SalI G'TCGA_C
Cuts at: 3234 3234
Size: 6197
SapI GCTCTTCn'nnn_
Cuts at: 4220 4430 4220
Size: 210 5987
SbfI CC_TGCA'GG
Cuts at: 3244 3244
Size: 6197
ScaI AGT'ACT
Cuts at: 929 3029 5714 929
Size: 2100 2685 1412
Fragments arranged by size:
2685 2100 1412
SfcI C'TryA_G
Cuts at: 33 833 1227 1775 3119 3240 3925 4918 5109
Size: 800 394 548 1344 121 685 993 191
Cuts at: 5109 33
Size: 1121
Fragments arranged by size:
1344 1121 993 800 685 548 394 191
121
SfoI GGC'GCC
Cuts at: 3629 3877 3629
Size: 248 5949
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6197
SmaI CCC'GGG
Cuts at: 3219 3219
Size: 6197
SmlI C'TyrA_G
Cuts at: 640 931 1597 1844 2290 2503 3352 4267 4759
Size: 291 666 247 446 213 849 915 492
Cuts at: 4759 5021 640
Size: 262 1816
Fragments arranged by size:
1816 915 849 666 492 446 291 262
247 213
SpeI A'CTAG_T
Cuts at: 67 3030 67
Size: 2963 3234
SphI G_CATG'C
Cuts at: 3250 3750 3250
Size: 500 5697
SspI AAT'ATT
Cuts at: 2485 2485
Size: 6197
StuI AGG'CCT
Cuts at: 509 509
Size: 6197
StyI C'CwwG_G
Cuts at: 504 518 1683 3051 3331 3685 504
Size: 14 1165 1368 280 354 3016
Fragments arranged by size:
3016 1368 1165 354 280 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3780 3913 4372 4581 3780
Size: 133 459 209 5396
Fragments arranged by size:
5396 459 209 133
TatI w'GTAC_w
Cuts at: 873 927 950 2085 2607 3027 5712 873
Size: 54 23 1135 522 420 2685 1358
Fragments arranged by size:
2685 1358 1135 522 420 54 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5714 5746 5714
Size: 32 6165
Tth111I GACn'n_nGTC
Cuts at: 3994 3994
Size: 6197
XbaI T'CTAG_A
Cuts at: 3228 3228
Size: 6197
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1710 3692 1710
Size: 1982 4215
XmaI C'CCGG_G
Cuts at: 3217 3217
Size: 6197
XmnI GAAnn'nnTTC
Cuts at: 115 448 611 2175 2285 2840 115
Size: 333 163 1564 110 555 3472
Fragments arranged by size:
3472 1564 555 333 163 110
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AscI BaeI BbvCI
BcgI BglI BmgBI BmtI Bpu10I BsgI BsiWI BsmBI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI FseI FspAI
HpaI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI