Restriction map for
pF1KA1122
(Linear) (Six-base) MAPSORT of: KIAA1122.seq Check: 2327 from: 1 to: 3084
KIAA1122.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 592 3084
Size: 592 2492
Acc65I G'GTAC_C
Cuts at: 0 2693 3084
Size: 2693 391
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 155 401 469 623 1918 2807 3084
Size: 140 15 246 68 154 1295 889 277
Fragments arranged by size:
1295 889 277 246 154 140 68 15
AflII C'TTAA_G
Cuts at: 0 532 3084
Size: 532 2552
AflIII A'CryG_T
Cuts at: 0 2030 2496 3084
Size: 2030 466 588
Fragments arranged by size:
2030 588 466
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1264 1296 3084
Size: 1264 32 1788
Fragments arranged by size:
1788 1264 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1090 3084
Size: 1090 1994
ApaI G_GGCC'C
Cuts at: 0 614 3084
Size: 614 2470
ApoI r'AATT_y
Cuts at: 0 661 784 991 1163 2013 2569 3084
Size: 661 123 207 172 850 556 515
Fragments arranged by size:
850 661 556 515 207 172 123
AseI AT'TA_AT
Cuts at: 0 1940 2525 3084
Size: 1940 585 559
Fragments arranged by size:
1940 585 559
AvrII C'CTAG_G
Cuts at: 0 1575 3084
Size: 1575 1509
BanI G'GyrC_C
Cuts at: 0 1700 2693 3084
Size: 1700 993 391
Fragments arranged by size:
1700 993 391
BanII G_rGCy'C
Cuts at: 0 614 1912 3084
Size: 614 1298 1172
Fragments arranged by size:
1298 1172 614
BbsI GAAGACnn'nnnn_
Cuts at: 0 275 452 470 887 1073 1394 3084
Size: 275 177 18 417 186 321 1690
Fragments arranged by size:
1690 417 321 275 186 177 18
BclI T'GATC_A
Cuts at: 0 672 3084
Size: 672 2412
BfrBI ATG'CAT
Cuts at: 0 2588 3084
Size: 2588 496
Bme1580I G_kGCm'C
Cuts at: 0 614 1705 3084
Size: 614 1091 1379
Fragments arranged by size:
1379 1091 614
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 838 3084
Size: 838 2246
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 3084
Size: 197 2887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 1474 1721 2167 2380 3084
Size: 808 666 247 446 213 704
Fragments arranged by size:
808 704 666 446 247 213
BsaAI yAC'GTr
Cuts at: 0 418 3084
Size: 418 2666
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1264 1294 3084
Size: 1264 30 1790
Fragments arranged by size:
1790 1264 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 3084
Size: 139 2945
BseYI C'CCAG_C
Cuts at: 0 83 3084
Size: 83 3001
BsmI GAATG_Cn'
Cuts at: 0 135 3084
Size: 135 2949
Bsp1286I G_dGCh'C
Cuts at: 0 614 1705 1912 3084
Size: 614 1091 207 1172
Fragments arranged by size:
1172 1091 614 207
BspHI T'CATG_A
Cuts at: 0 2327 3084
Size: 2327 757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 592 3084
Size: 592 2492
BsrDI GCAATG_nn'
Cuts at: 0 1595 2231 2328 3084
Size: 1595 636 97 756
Fragments arranged by size:
1595 756 636 97
BsrGI T'GTAC_A
Cuts at: 0 842 1977 3084
Size: 842 1135 1107
Fragments arranged by size:
1135 1107 842
BstAPI GCAn_nnn'nTGC
Cuts at: 0 574 3084
Size: 574 2510
BstBI TT'CG_AA
Cuts at: 0 2489 3084
Size: 2489 595
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2652 3084
Size: 2652 432
BtgI C'CryG_G
Cuts at: 0 410 3084
Size: 410 2674
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2353 3084
Size: 2353 731
DraIII CAC_nnn'GTG
Cuts at: 0 910 2437 3084
Size: 910 1527 647
Fragments arranged by size:
1527 910 647
EaeI y'GGCC_r
Cuts at: 0 2348 3084
Size: 2348 736
EarI CTCTTCn'nnn_
Cuts at: 0 116 383 2157 2168 3084
Size: 116 267 1774 11 916
Fragments arranged by size:
1774 916 267 116 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 155 197 401 469 623 1918 2807
Size: 140 15 42 204 68 154 1295 889
Cuts at: 2807 3084
Size: 277
Fragments arranged by size:
1295 889 277 204 154 140 68 42
15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1618 3084
Size: 1618 1466
EcoO109I rG'GnC_Cy
Cuts at: 0 1716 3084
Size: 1716 1368
EcoRV GAT'ATC
Cuts at: 0 2658 2769 3084
Size: 2658 111 315
Fragments arranged by size:
2658 315 111
HaeII r_GCGC'y
Cuts at: 0 61 3084
Size: 61 3023
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 376 408 661 693 3084
Size: 376 32 253 32 2391
Fragments arranged by size:
2391 376 253 32 32
HindIII A'AGCT_T
Cuts at: 0 851 3084
Size: 851 2233
KpnI G_GTAC'C
Cuts at: 0 2697 3084
Size: 2697 387
MfeI C'AATT_G
Cuts at: 0 569 1190 3084
Size: 569 621 1894
Fragments arranged by size:
1894 621 569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2210 3084
Size: 2210 874
MscI TGG'CCA
Cuts at: 0 2350 3084
Size: 2350 734
MslI CAynn'nnrTG
Cuts at: 0 1288 1538 2314 3084
Size: 1288 250 776 770
Fragments arranged by size:
1288 776 770 250
MspA1I CmG'CkG
Cuts at: 0 2386 3084
Size: 2386 698
NcoI C'CATG_G
Cuts at: 0 410 3084
Size: 410 2674
NdeI CA'TA_TG
Cuts at: 0 648 1892 3084
Size: 648 1244 1192
Fragments arranged by size:
1244 1192 648
NsiI A_TGCA'T
Cuts at: 0 2590 3084
Size: 2590 494
NspI r_CATG'y
Cuts at: 0 2034 2131 2500 3084
Size: 2034 97 369 584
Fragments arranged by size:
2034 584 369 97
PciI A'CATG_T
Cuts at: 0 2030 2496 3084
Size: 2030 466 588
Fragments arranged by size:
2030 588 466
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1264 1296 3084
Size: 1264 32 1788
Fragments arranged by size:
1788 1264 32
PpuMI rG'GwC_Cy
Cuts at: 0 1716 3084
Size: 1716 1368
PshAI GACnn'nnGTC
Cuts at: 0 1841 3084
Size: 1841 1243
PsiI TTA'TAA
Cuts at: 0 1366 2143 2867 2929 3084
Size: 1366 777 724 62 155
Fragments arranged by size:
1366 777 724 155 62
PspOMI G'GGCC_C
Cuts at: 0 610 3084
Size: 610 2474
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2483 2515 3084
Size: 2483 32 569
Fragments arranged by size:
2483 569 32
PstI C_TGCA'G
Cuts at: 0 729 3084
Size: 729 2355
PvuII CAG'CTG
Cuts at: 0 2386 3084
Size: 2386 698
ScaI AGT'ACT
Cuts at: 0 821 2921 3084
Size: 821 2100 163
Fragments arranged by size:
2100 821 163
SfcI C'TryA_G
Cuts at: 0 725 1119 1667 3011 3084
Size: 725 394 548 1344 73
Fragments arranged by size:
1344 725 548 394 73
SmlI C'TyrA_G
Cuts at: 0 532 823 1489 1736 2182 2395 3084
Size: 532 291 666 247 446 213 689
Fragments arranged by size:
689 666 532 446 291 247 213
SpeI A'CTAG_T
Cuts at: 0 2922 3084
Size: 2922 162
SspI AAT'ATT
Cuts at: 0 2377 3084
Size: 2377 707
StuI AGG'CCT
Cuts at: 0 401 3084
Size: 401 2683
StyI C'CwwG_G
Cuts at: 0 396 410 1575 2943 3084
Size: 396 14 1165 1368 141
Fragments arranged by size:
1368 1165 396 141 14
TatI w'GTAC_w
Cuts at: 0 765 819 842 1977 2499 2919 3084
Size: 765 54 23 1135 522 420 165
Fragments arranged by size:
1135 765 522 420 165 54 23
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1602 3084
Size: 1602 1482
XmnI GAAnn'nnTTC
Cuts at: 0 7 340 503 2067 2177 2732 3084
Size: 7 333 163 1564 110 555 352
Fragments arranged by size:
1564 555 352 333 163 110 7
Enzymes that do cut:
AarI Acc65I AcuI AflII AflIII AloI AlwNI ApaI
ApoI AseI AvrII BanI BanII BbsI BclI BfrBI
Bme1580I BplI BpmI BpuEI BsaAI BsaXI BseRI BseYI
BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI BstBI
BstXI BtgI BtgZI DraIII EaeI EarI Eco57MI EcoNI
EcoO109I EcoRV HaeII Hin4I HindIII KpnI MfeI MmeI
MscI MslI MspA1I NcoI NdeI NsiI NspI PciI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuII
ScaI SfcI SmlI SpeI SspI StuI StyI TatI
XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AgeI AhdI AleI AlfI
ApaLI AscI AsiSI AvaI BaeI BamHI BbeI BbvCI
BcgI BciVI BglI BglII BlpI BmgBI BmrI BmtI
Bpu10I BsaI BsaBI BsaHI BsaWI BsgI BsiEI BsiHKAI
BsiWI BsmBI BspEI BsrBI BsrFI BssHII BssSI BstEII
BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI
EagI EciI EcoICRI EcoRI FalI FseI FspI FspAI
HincII HpaI KasI MluI NaeI NarI NgoMIV NheI
NotI NruI PacI PasI PflMI PfoI PmeI PmlI
PspXI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI SwaI TaqII TstI Tth111I XbaI XhoI
XmaI ZraI