Restriction map for
pF1KA1115
(Linear) (Six-base) MAPSORT of: KIAA1115.seq Check: 7222 from: 1 to: 2643
KIAA1115.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 167 2643
Size: 167 2476
AatII G_ACGT'C
Cuts at: 0 1336 2643
Size: 1336 1307
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 858 1492 2395 2643
Size: 354 504 634 903 248
Fragments arranged by size:
903 634 504 354 248
AflII C'TTAA_G
Cuts at: 0 753 2643
Size: 753 1890
AflIII A'CryG_T
Cuts at: 0 761 2643
Size: 761 1882
AleI CACnn'nnGTG
Cuts at: 0 514 2643
Size: 514 2129
AlwNI CAG_nnn'CTG
Cuts at: 0 582 642 703 813 990 1470 2268 2643
Size: 582 60 61 110 177 480 798 375
Fragments arranged by size:
798 582 480 375 177 110 61 60
ApaI G_GGCC'C
Cuts at: 0 918 1445 1526 1962 2234 2502 2643
Size: 918 527 81 436 272 268 141
Fragments arranged by size:
918 527 436 272 268 141 81
AvaI C'yCGr_G
Cuts at: 0 73 2180 2643
Size: 73 2107 463
Fragments arranged by size:
2107 463 73
BamHI G'GATC_C
Cuts at: 0 803 1325 2429 2643
Size: 803 522 1104 214
Fragments arranged by size:
1104 803 522 214
BanI G'GyrC_C
Cuts at: 0 289 472 943 1414 1927 2643
Size: 289 183 471 471 513 716
Fragments arranged by size:
716 513 471 471 289 183
BanII G_rGCy'C
Cuts at: 0 833 902 918 1130 1445 1526 1903 1962
Size: 833 69 16 212 315 81 377 59
Cuts at: 1962 2234 2372 2502 2539 2546 2643
Size: 272 138 130 37 7 97
Fragments arranged by size:
833 377 315 272 212 138 130 97
81 69 59 37 16 7
BbeI G_GCGC'C
Cuts at: 0 947 1931 2643
Size: 947 984 712
Fragments arranged by size:
984 947 712
BbsI GAAGACnn'nnnn_
Cuts at: 0 98 1173 2051 2643
Size: 98 1075 878 592
Fragments arranged by size:
1075 878 592 98
BbvCI CC'TCA_GC
Cuts at: 0 527 532 2114 2328 2373 2643
Size: 527 5 1582 214 45 270
Fragments arranged by size:
1582 527 270 214 45 5
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 747 781 2643
Size: 747 34 1862
Fragments arranged by size:
1862 747 34
BglI GCCn_nnn'nGGC
Cuts at: 0 837 913 1957 2497 2550 2643
Size: 837 76 1044 540 53 93
Fragments arranged by size:
1044 837 540 93 76 53
BlpI GC'TnA_GC
Cuts at: 0 970 1231 2643
Size: 970 261 1412
Fragments arranged by size:
1412 970 261
Bme1580I G_kGCm'C
Cuts at: 0 269 918 1095 1148 1419 1445 1526 1962
Size: 269 649 177 53 271 26 81 436
Cuts at: 1962 2234 2246 2502 2574 2643
Size: 272 12 256 72 69
Fragments arranged by size:
649 436 272 271 269 256 177 81
72 69 53 26 12
BmgBI CAC'GTC
Cuts at: 0 96 1143 2643
Size: 96 1047 1500
Fragments arranged by size:
1500 1047 96
BmrI ACTGGGnnnn_n'
Cuts at: 0 227 630 2146 2219 2643
Size: 227 403 1516 73 424
Fragments arranged by size:
1516 424 403 227 73
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 745 847 916 1015 1030 1572 2105
Size: 70 675 102 69 99 15 542 533
Cuts at: 2105 2201 2643
Size: 96 442
Fragments arranged by size:
675 542 533 442 102 99 96 70
69 15
Bpu10I CC'TnA_GC
Cuts at: 0 64 527 532 661 1096 1902 2114 2302
Size: 64 463 5 129 435 806 212 188
Cuts at: 2302 2328 2373 2643
Size: 26 45 270
Fragments arranged by size:
806 463 435 270 212 188 129 64
45 26 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1597 1846 2643
Size: 1597 249 797
Fragments arranged by size:
1597 797 249
BsaI GGTCTCn'nnnn_
Cuts at: 0 1528 2312 2462 2643
Size: 1528 784 150 181
Fragments arranged by size:
1528 784 181 150
BsaAI yAC'GTr
Cuts at: 0 1071 2643
Size: 1071 1572
BsaBI GATnn'nnATC
Cuts at: 0 614 2643
Size: 614 2029
BsaHI Gr'CG_yC
Cuts at: 0 944 1267 1333 1928 2291 2643
Size: 944 323 66 595 363 352
Fragments arranged by size:
944 595 363 352 323 66
BsaWI w'CCGG_w
Cuts at: 0 1985 2643
Size: 1985 658
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1567 1597 2199 2229 2574 2604 2643
Size: 1567 30 602 30 345 30 39
Fragments arranged by size:
1567 602 345 39 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 223 577 1357 1575 1648 1735 1753 1891
Size: 223 354 780 218 73 87 18 138
Cuts at: 1891 2038 2047 2050 2053 2065 2439 2579 2643
Size: 147 9 3 3 12 374 140 64
Fragments arranged by size:
780 374 354 223 218 147 140 138
87 73 64 18 12 9 3 3
BseYI C'CCAG_C
Cuts at: 0 1483 2101 2128 2443 2487 2643
Size: 1483 618 27 315 44 156
Fragments arranged by size:
1483 618 315 156 44 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 1051 1444 2087 2188 2643
Size: 4 1047 393 643 101 455
Fragments arranged by size:
1047 643 455 393 101 4
BsiEI CG_ry'CG
Cuts at: 0 1353 1608 2643
Size: 1353 255 1035
Fragments arranged by size:
1353 1035 255
BsiHKAI G_wGCw'C
Cuts at: 0 337 902 1130 1225 2643
Size: 337 565 228 95 1418
Fragments arranged by size:
1418 565 337 228 95
BsmBI CGTCTCn'nnnn_
Cuts at: 0 730 1258 2643
Size: 730 528 1385
Fragments arranged by size:
1385 730 528
Bsp1286I G_dGCh'C
Cuts at: 0 269 337 833 902 918 1095 1130 1148
Size: 269 68 496 69 16 177 35 18
Cuts at: 1148 1225 1419 1445 1526 1903 1962 2234 2246
Size: 77 194 26 81 377 59 272 12
Cuts at: 2246 2372 2502 2539 2546 2574 2643
Size: 126 130 37 7 28 69
Fragments arranged by size:
496 377 272 269 194 177 130 126
81 77 69 69 68 59 37 35
28 26 18 16 12 7
BspEI T'CCGG_A
Cuts at: 0 1985 2643
Size: 1985 658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 26 167 461 2643
Size: 26 141 294 2182
Fragments arranged by size:
2182 294 141 26
BsrBI CCG'CTC
Cuts at: 0 54 213 522 1323 2643
Size: 54 159 309 801 1320
Fragments arranged by size:
1320 801 309 159 54
BsrDI GCAATG_nn'
Cuts at: 0 173 2643
Size: 173 2470
BsrFI r'CCGG_y
Cuts at: 0 311 337 1411 1607 2643
Size: 311 26 1074 196 1036
Fragments arranged by size:
1074 1036 311 196 26
BsrGI T'GTAC_A
Cuts at: 0 1356 2643
Size: 1356 1287
BssSI C'ACGA_G
Cuts at: 0 415 1123 2643
Size: 415 708 1520
Fragments arranged by size:
1520 708 415
BstEII G'GTnAC_C
Cuts at: 0 180 1393 2643
Size: 180 1213 1250
Fragments arranged by size:
1250 1213 180
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1978 2643
Size: 1978 665
BstYI r'GATC_y
Cuts at: 0 593 803 1325 2429 2643
Size: 593 210 522 1104 214
Fragments arranged by size:
1104 593 522 214 210
Bsu36I CC'TnA_GG
Cuts at: 0 1631 2336 2643
Size: 1631 705 307
Fragments arranged by size:
1631 705 307
BtgI C'CryG_G
Cuts at: 0 964 2073 2424 2643
Size: 964 1109 351 219
Fragments arranged by size:
1109 964 351 219
BtsI GCAGTG_nn'
Cuts at: 0 352 885 1312 1507 2643
Size: 352 533 427 195 1136
Fragments arranged by size:
1136 533 427 352 195
DraIII CAC_nnn'GTG
Cuts at: 0 1981 2643
Size: 1981 662
EaeI y'GGCC_r
Cuts at: 0 359 479 716 1085 1409 1949 2270 2643
Size: 359 120 237 369 324 540 321 373
Fragments arranged by size:
540 373 369 359 324 321 237 120
EarI CTCTTCn'nnn_
Cuts at: 0 1171 1868 1883 2015 2192 2527 2643
Size: 1171 697 15 132 177 335 116
Fragments arranged by size:
1171 697 335 177 132 116 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 646 1034 1546 2643
Size: 646 388 512 1097
Fragments arranged by size:
1097 646 512 388
EcoICRI GAG'CTC
Cuts at: 0 900 1128 2643
Size: 900 228 1515
Fragments arranged by size:
1515 900 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 354 745 847 858 916 1015 1030
Size: 70 284 391 102 11 58 99 15
Cuts at: 1030 1492 1572 2105 2201 2395 2643
Size: 462 80 533 96 194 248
Fragments arranged by size:
533 462 391 284 248 194 102 99
96 80 70 58 15 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1379 2643
Size: 1379 1264
EcoO109I rG'GnC_Cy
Cuts at: 0 60 914 915 1372 1441 1522 1523 1958
Size: 60 854 1 457 69 81 1 435
Cuts at: 1958 2125 2230 2231 2250 2313 2406 2498 2577
Size: 167 105 1 19 63 93 92 79
Cuts at: 2577 2620 2643
Size: 43 23
Fragments arranged by size:
854 457 435 167 105 93 92 81
79 69 63 60 43 23 19 1
1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 459 2643
Size: 459 2184
HaeII r_GCGC'y
Cuts at: 0 947 1103 1931 2643
Size: 947 156 828 712
Fragments arranged by size:
947 828 712 156
HincII GTy'rAC
Cuts at: 0 120 2643
Size: 120 2523
KasI G'GCGC_C
Cuts at: 0 943 1927 2643
Size: 943 984 716
Fragments arranged by size:
984 943 716
MfeI C'AATT_G
Cuts at: 0 549 2643
Size: 549 2094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 2643
Size: 650 1993
MscI TGG'CCA
Cuts at: 0 361 718 1087 1951 2272 2643
Size: 361 357 369 864 321 371
Fragments arranged by size:
864 371 369 361 357 321
MslI CAynn'nnrTG
Cuts at: 0 514 2643
Size: 514 2129
MspA1I CmG'CkG
Cuts at: 0 531 579 894 966 974 1458 1467 1947
Size: 531 48 315 72 8 484 9 480
Cuts at: 1947 2132 2643
Size: 185 511
Fragments arranged by size:
531 511 484 480 315 185 72 48
9 8
NarI GG'CG_CC
Cuts at: 0 944 1928 2643
Size: 944 984 715
Fragments arranged by size:
984 944 715
NspI r_CATG'y
Cuts at: 0 765 1038 1201 2643
Size: 765 273 163 1442
Fragments arranged by size:
1442 765 273 163
PasI CC'CwG_GG
Cuts at: 0 285 1896 2247 2643
Size: 285 1611 351 396
Fragments arranged by size:
1611 396 351 285
PciI A'CATG_T
Cuts at: 0 761 2643
Size: 761 1882
PflMI CCAn_nnn'nTGG
Cuts at: 0 714 2391 2643
Size: 714 1677 252
Fragments arranged by size:
1677 714 252
PfoI T'CCnGG_A
Cuts at: 0 686 2643
Size: 686 1957
PmlI CAC'GTG
Cuts at: 0 1071 2643
Size: 1071 1572
PpuMI rG'GwC_Cy
Cuts at: 0 60 2313 2406 2643
Size: 60 2253 93 237
Fragments arranged by size:
2253 237 93 60
PspOMI G'GGCC_C
Cuts at: 0 914 1441 1522 1958 2230 2498 2643
Size: 914 527 81 436 272 268 145
Fragments arranged by size:
914 527 436 272 268 145 81
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 301 333 2643
Size: 301 32 2310
Fragments arranged by size:
2310 301 32
PstI C_TGCA'G
Cuts at: 0 104 149 332 464 1304 1649 2300 2643
Size: 104 45 183 132 840 345 651 343
Fragments arranged by size:
840 651 345 343 183 132 104 45
PvuII CAG'CTG
Cuts at: 0 531 894 974 1458 1467 1947 2132 2643
Size: 531 363 80 484 9 480 185 511
Fragments arranged by size:
531 511 484 480 363 185 80 9
SacI G_AGCT'C
Cuts at: 0 902 1130 2643
Size: 902 228 1513
Fragments arranged by size:
1513 902 228
SacII CC_GC'GG
Cuts at: 0 967 2643
Size: 967 1676
SapI GCTCTTCn'nnn_
Cuts at: 0 2192 2527 2643
Size: 2192 335 116
Fragments arranged by size:
2192 335 116
SexAI A'CCwGG_T
Cuts at: 0 1559 1977 2643
Size: 1559 418 666
Fragments arranged by size:
1559 666 418
SfcI C'TryA_G
Cuts at: 0 100 145 328 460 1018 1300 1645 2159
Size: 100 45 183 132 558 282 345 514
Cuts at: 2159 2296 2643
Size: 137 347
Fragments arranged by size:
558 514 347 345 282 183 137 132
100 45
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 913 1957 2643
Size: 913 1044 686
Fragments arranged by size:
1044 913 686
SfoI GGC'GCC
Cuts at: 0 945 1929 2643
Size: 945 984 714
Fragments arranged by size:
984 945 714
SmlI C'TyrA_G
Cuts at: 0 753 1612 1825 2643
Size: 753 859 213 818
Fragments arranged by size:
859 818 753 213
SphI G_CATG'C
Cuts at: 0 1038 1201 2643
Size: 1038 163 1442
Fragments arranged by size:
1442 1038 163
StuI AGG'CCT
Cuts at: 0 1654 2389 2643
Size: 1654 735 254
Fragments arranged by size:
1654 735 254
StyI C'CwwG_G
Cuts at: 0 909 2390 2546 2643
Size: 909 1481 156 97
Fragments arranged by size:
1481 909 156 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 199 277 1585 2643
Size: 199 78 1308 1058
Fragments arranged by size:
1308 1058 199 78
TatI w'GTAC_w
Cuts at: 0 1356 2643
Size: 1356 1287
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1567 1599 1967 1999 2199 2231 2574 2606
Size: 1567 32 368 32 200 32 343 32
Cuts at: 2606 2643
Size: 37
Fragments arranged by size:
1567 368 343 200 37 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1155 1233 2129 2265 2643
Size: 1155 78 896 136 378
Fragments arranged by size:
1155 896 378 136 78
XmnI GAAnn'nnTTC
Cuts at: 0 868 2643
Size: 868 1775
ZraI GAC'GTC
Cuts at: 0 1334 2643
Size: 1334 1309
Enzymes that do cut:
AarI AatII AcuI AflII AflIII AleI AlwNI ApaI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstEII BstXI BstYI Bsu36I
BtgI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII HincII KasI MfeI MmeI
MscI MslI MspA1I NarI NspI PasI PciI PflMI
PfoI PmlI PpuMI PspOMI PsrI PstI PvuII SacI
SacII SapI SexAI SfcI SfiI SfoI SmlI SphI
StuI StyI TaqII TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AgeI AhdI AlfI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BciVI
BclI BfrBI BglII BmtI BplI BsiWI BsmI BspHI
BssHII BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI
DrdI EagI EcoRI EcoRV FseI FspI FspAI Hin4I
HindIII HpaI KpnI MluI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PmeI PpiI PshAI
PsiI PspXI PvuI RsrII SalI SanDI SbfI ScaI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI XhoI XmaI