Restriction map for pF1KA0901
##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

AatII       AclI        AflII       AgeI        AhdI        
Aor51HI     AscI        AseI        AsnI        Asp700I     
AspEI       AviII       BbeI        BciVI       BfrI        
BglII       BsaBI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BsrGI       BssHII      Bst1107I    
BstZ17I     ClaI        CpoI        DraI        Eam1105I    
Eco47III    EcoT22I     FseI        FspI        HindIII     
HpaI        KasI        KspI        MamI        MfeI        
MluI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
PacI        PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SfiI        SfoI        
SgrAI       SnaBI       SpeI        Sse8387I    SspBI       
SwaI        XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I       1506  GGTACC               G^GTACC
AccI         3451  GTCTAC               GT^[AC][GT]AC
AcyI         1180  GGCGTC               G[GA]^CG[CT]C
AflIII       1973  ACATGT               A^C[GA][CT]GT
Asp718I      1506  GGTACC               G^GTACC
AspI         3018  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        2581  CCTAGG               C^CTAGG
BbrPI         688  CACGTG               CAC^GTG
BlnI         2581  CCTAGG               C^CTAGG
BsaHI        1180  GGCGTC               G[GA]^CG[CT]C
BsiEI        2073  CGGCCG               CG[GA][CT]^CG
BspHI        2507  TCATGA               T^CATGA
BspLU11I     1973  ACATGT               A^CATGT
BsrFI          10  ACCGGC               [GA]^CCGG[CT]
BssSI        2606  CACGAG               CACGAG(-5/-1)
BstBI          90  TTCGAA               TT^CGAA
BstEII       2550  GGTCACC              G^GT[ACGT]ACC
BstPI        2550  GGTCACC              G^GT[ACGT]ACC
Cfr10I         10  ACCGGC               [GA]^CCGG[CT]
DraIII       1235  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         2073  CGGCCG               C^GGCCG
EclXI        2073  CGGCCG               C^GGCCG
Eco52I       2073  CGGCCG               C^GGCCG
EcoO65I      2550  GGTCACC              G^GT[ACGT]ACC
EcoRI         286  GAATTC               G^AATTC
EcoRV        3129  GATATC               GAT^ATC
Hin1I        1180  GGCGTC               G[GA]^CG[CT]C
HincII       2101  GTCAAC               GT[CT]^[GA]AC
HindII       2101  GTCAAC               GT[CT]^[GA]AC
KpnI         1506  GGTACC               GGTAC^C
NspV           90  TTCGAA               TT^CGAA
PflFI        3018  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         688  CACGTG               CAC^GTG
PmlI          688  CACGTG               CAC^GTG
PstI         3368  CTGCAG               CTGCA^G
RcaI         2507  TCATGA               T^CATGA
SapI          415  GAAGAGC              GCTCTTC(1/4)
SfuI           90  TTCGAA               TT^CGAA
SmaI         1667  CCCGGG               CCC^GGG
SphI         1914  GCATGC               GCATG^C
SspI          100  AATATT               AAT^ATT
Tth111I      3018  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        1667  CCCGGG               C^CCGGG
XmaI         1667  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccII        1165  CGCG                 CG^CG
             1583  CGCG                
AcsI          286  GAATTC               [GA]^AATT[CT]
             3498  AAATTC              
Alw44I        760  GTGCAC               G^TGCAC
             1733  GTGCAC              
ApaI         2119  GGGCCC               GGGCC^C
             2444  GGGCCC              
ApaLI         760  GTGCAC               G^TGCAC
             1733  GTGCAC              
ApoI          286  GAATTC               [GA]^AATT[CT]
             3498  AAATTC              
BamHI        1925  GGATCC               G^GATCC
             2238  GGATCC              
BbsI          193  GAAGAC               GAAGAC(2/6)
             2518  GAAGAC              
BbvCI        1006  CCTCAGC              CCTCAGC(-5/-2)
             3519  CCTCAGC             
BcgI         3266  CGATGCTGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2063  GCAGCCAGATCG        
BclI         2022  TGATCA               T^GATCA
             3297  TGATCA              
BlpI         1600  GCTGAGC              GC^T[ACGT]AGC
             2590  GCTGAGC             
Bpu1102I     1600  GCTGAGC              GC^T[ACGT]AGC
             2590  GCTGAGC             
BpuAI         193  GAAGAC               GAAGAC(2/6)
             2518  GAAGAC              
BsaAI         688  CACGTG               [CT]AC^GT[GA]
             1630  TACGTG              
BstAPI        972  GCACGTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1735  GCACAGCTTGC         
BstUI        1165  CGCG                 CG^CG
             1583  CGCG                
CelII        1600  GCTGAGC              GC^T[ACGT]AGC
             2590  GCTGAGC             
DrdI           76  GACTCCAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              799  GACCCCAGTGTC        
EarI          415  GAAGAG               CTCTTC(1/4)
             2665  GAAGAG              
FbaI         2022  TGATCA               T^GATCA
             3297  TGATCA              
HaeII        1562  GGCGCT               [GA]GCGC^[CT]
             1750  GGCGCT              
HgaI         1181  GCGTC                GACGC(5/10)
             1815  GCGTC               
Ksp632I       415  GAAGAG               CTCTTC(1/4)
             2665  GAAGAG              
MvnI         1165  CGCG                 CG^CG
             1583  CGCG                
PaeR7I        994  CTCGAG               C^TCGAG
             3072  CTCGAG              
PflMI         180  CCAAGCAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3342  CCATTTGGTGG         
SexAI         929  ACCAGGT              A^CC[AT]GGT
             1236  ACCTGGT             
Van91I        180  CCAAGCAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3342  CCATTTGGTGG         
XhoI          994  CTCGAG               C^TCGAG
             3072  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AccIII      AciI        AfaI        AluI        AlwI        
AlwNI       AocI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspEI       BspMI       BsrBI       
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstXI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        Eco81I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MroI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII       
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