Restriction map for pF1KA0892
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# NO CUT ENZYMES (   93 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AflII       
AgeI        AocI        Aor51HI     ApaI        AscI        
AseI        AsnI        Asp700I     AspI        AvrII       
BamHI       BbeI        BcgI        BciVI       BclI        
BfaI        BfrI        BlnI        BsaHI       BsaWI       
BseAI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BsrBI       BsrGI       
Bst1107I    BstXI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraIII      DrdI        Eco47III    
Eco81I      EcoT22I     FbaI        FseI        Hin1I       
HindIII     HpaI        KasI        KspI        MaeI        
MfeI        MluI        MroI        MunI        NarI        
NdeI        NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        ScaI        
SfoI        SgrAI       SnaBI       SpeI        SphI        
SspBI       SspI        SwaI        TfiI        Tth111I     
XbaI        XhoI        XmnI        


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# ONE CUT ENZYMES (   56 enzymes) #
###################################

Acc65I         74  GGTACC               G^GTACC
AccI          874  GTCTAC               GT^[AC][GT]AC
Asp718I        74  GGTACC               G^GTACC
AvaI          524  CCCGGG               C^[CT]CG[GA]G
AviII          48  TGCGCA               TGC^GCA
BalI         1826  TGGCCA               TGG^CCA
BbrPI        1764  CACGTG               CAC^GTG
BbsI          688  GTCTTC               GAAGAC(2/6)
BlpI           97  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I       97  GCTGAGC              GC^T[ACGT]AGC
BpuAI         688  GTCTTC               GAAGAC(2/6)
BsaAI        1764  CACGTG               [CT]AC^GT[GA]
BsaBI         399  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1637  GAGACC               GGTCTC(1/5)
BseRI         562  CTCCTC               GAGGAG(10/8)
BsiEI          62  CGGCCG               CG[GA][CT]^CG
BsoBI         524  CCCGGG               C^[CT]CG[GA]G
BsrDI        1148  CATTGC               GCAATG(2/0)
BssHII        253  GCGCGC               G^CGCGC
BstBI         304  TTCGAA               TT^CGAA
BstEII        708  GGTCACC              G^GT[ACGT]ACC
BstPI         708  GGTCACC              G^GT[ACGT]ACC
CelII          97  GCTGAGC              GC^T[ACGT]AGC
EagI           62  CGGCCG               C^GGCCG
EclXI          62  CGGCCG               C^GGCCG
Eco52I         62  CGGCCG               C^GGCCG
EcoO65I       708  GGTCACC              G^GT[ACGT]ACC
EcoRI         360  GAATTC               G^AATTC
EcoRV         282  GATATC               GAT^ATC
FspI           48  TGCGCA               TGC^GCA
HgaI         1418  GACGC                GACGC(5/10)
HincII       1174  GTCAAC               GT[CT]^[GA]AC
HindII       1174  GTCAAC               GT[CT]^[GA]AC
KpnI           74  GGTACC               GGTAC^C
MaeII        1765  ACGT                 A^CGT
MamI          399  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1826  TGGCCA               TGG^CCA
MscI         1826  TGGCCA               TGG^CCA
MseI          314  TTAA                 T^TAA
NaeI          907  GCCGGC               GCC^GGC
NcoI         1664  CCATGG               C^CATGG
NgoMIV        907  GCCGGC               G^CCGGC
NspV          304  TTCGAA               TT^CGAA
PflMI         714  CCACTATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1764  CACGTG               CAC^GTG
PmlI         1764  CACGTG               CAC^GTG
SbfI          150  CCTGCAGG             CCTGCA^GG
SfiI           54  GGCCGAGGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI          304  TTCGAA               TT^CGAA
SmaI          524  CCCGGG               CCC^GGG
Sse8387I      150  CCTGCAGG             CCTGCA^GG
StuI         1733  AGGCCT               AGG^CCT
Tru9I         314  TTAA                 T^TAA
Van91I        714  CCACTATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         524  CCCGGG               C^CCGGG
XmaI          524  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AccII         254  CGCG                 CG^CG
             1191  CGCG                
AcsI          316  AAATTT               [GA]^AATT[CT]
              360  GAATTC              
AflIII        860  ACATGT               A^C[GA][CT]GT
             1601  ACATGT              
AhdI          478  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1770  GACAGACGGTC         
Alw44I        142  GTGCAC               G^TGCAC
              610  GTGCAC              
AlwNI        1234  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1708  CAGCAGCTG           
ApaLI         142  GTGCAC               G^TGCAC
              610  GTGCAC              
ApoI          316  AAATTT               [GA]^AATT[CT]
              360  GAATTC              
AspEI         478  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1770  GACAGACGGTC         
BanI           74  GGTACC               G^G[CT][GA]CC
             1566  GGTGCC              
BanII         213  GGGCTC               G[GA]GC[CT]^C
             1396  GGGCTC              
BbvCI         564  CCTCAGC              CCTCAGC(-5/-2)
             1471  GCTGAGG             
BglI           25  GCCCAGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
               55  GCCGAGGCGGC         
BglII         404  AGATCT               A^GATCT
             1076  AGATCT              
BsmAI        1637  GAGAC                GTCTC(1/5)
             1168  GTCTC               
BsmFI        1416  GGGAC                GGGAC(10/14)
             1107  GTCCC               
BspLU11I      860  ACATGT               A^CATGT
             1601  ACATGT              
BspMI         203  ACCTGC               ACCTGC(4/8)
              732  GCAGGT              
BsrFI         907  GCCGGC               [GA]^CCGG[CT]
             1484  ACCGGC              
BssSI          69  CTCGTG               CACGAG(-5/-1)
             1504  CTCGTG              
BstUI         254  CGCG                 CG^CG
             1191  CGCG                
Cfr10I        907  GCCGGC               [GA]^CCGG[CT]
             1484  ACCGGC              
EaeI           62  CGGCCG               [CT]^GGCC[GA]
             1826  TGGCCA              
Eam1105I      478  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1770  GACAGACGGTC         
EcoT14I       851  CCAAGG               C^C[AT][AT]GG
             1664  CCATGG              
HinfI          67  GACTC                G^A[ACGT]TC
              676  GAGTC               
MvnI          254  CGCG                 CG^CG
             1191  CGCG                
NspI          860  ACATGT               [GA]CATG^[CT]
             1601  ACATGT              
PleI           67  GACTC                GAGTC(4/5)
              676  GAGTC               
PpuMI         476  AGGACCT              [GA]G^G[AT]CC[CT]
             1475  AGGACCT             
SexAI         479  ACCTGGT              A^CC[AT]GGT
              878  ACCTGGT             
SmlI          469  CTTGAG               C^T[CT][GA]AG
              967  CTCAAG              
StyI          851  CCAAGG               C^C[AT][AT]GG
             1664  CCATGG              
TaqI          188  TCGA                 T^CGA
              305  TCGA                
TthHB8I       188  TCGA                 T^CGA
              305  TCGA                
XcmI         1797  CCAAGCTCAGAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1814  CCAACACCAGCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AvaII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BsgI        BsiHKAI     BsiYI       BslI        
Bsp1286I    BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EarI        
EcoNI       EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HpaII       HphI        ItaI        Ksp632I     
MaeIII      MboI        MboII       MflI        MnlI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PstI        
PvuII       RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TseI        Tsp45I      
Tsp509I     TspRI       XhoII       
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