Restriction map for pF1KA0882
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       AclI        AcyI        AflII       AgeI        
AhdI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp700I     AspEI       AviII       AvrII       
BamHI       BbeI        BbrPI       BfrI        BglII       
BlnI        BsaBI       BsaHI       BsiEI       BsiWI       
BspHI       BspMI       BssHII      Bst1107I    BstEII      
BstPI       BstZ17I     CpoI        DraIII      EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       EcoT22I     FseI        FspI        
Hin1I       HpaI        KasI        KspI        MamI        
MluI        NarI        NdeI        NotI        NruI        
NsiI        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        SexAI       SfoI        SnaBI       SpeI        
Sse8387I    StuI        SwaI        XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

Acc65I        142  GGTACC               G^GTACC
AccIII       3737  TCCGGA               T^CCGGA
AocI         2296  CCTGAGG              CC^T[ACGT]AGG
Asp718I       142  GGTACC               G^GTACC
BbvCI         540  CCTCAGC              CCTCAGC(-5/-2)
BciVI        2415  GGATAC               GTATCC(6/5)
BlpI         2271  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2271  GCTCAGC              GC^T[ACGT]AGC
BsaAI        3313  TACGTG               [CT]AC^GT[GA]
BseAI        3737  TCCGGA               T^CCGGA
BsmBI        1290  CGTCTC               CGTCTC(1/5)
BspDI         794  ATCGAT               AT^CGAT
BspEI        3737  TCCGGA               T^CCGGA
BstAPI       2799  GCACGTCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1775  TTCGAA               TT^CGAA
BstXI        1910  CCAGAGTTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       2296  CCTGAGG              CC^T[ACGT]AGG
CelII        2271  GCTCAGC              GC^T[ACGT]AGC
ClaI          794  ATCGAT               AT^CGAT
DraI          421  TTTAAA               TTT^AAA
Eco81I       2296  CCTGAGG              CC^T[ACGT]AGG
EcoRV         328  GATATC               GAT^ATC
HindIII      1853  AAGCTT               A^AGCTT
KpnI          142  GGTACC               GGTAC^C
MfeI         1064  CAATTG               C^AATTG
MroI         3737  TCCGGA               T^CCGGA
MunI         1064  CAATTG               C^AATTG
NheI         1953  GCTAGC               G^CTAGC
NspV         1775  TTCGAA               TT^CGAA
PflMI        2403  CCAGACGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI           76  CTGCAG               CTGCA^G
SfcI           76  CTGCAG               C^T[GA][CT]AG
SfiI         3342  GGCCAGCCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI         1775  TTCGAA               TT^CGAA
SgrAI          96  CGCCGGCG             C[GA]^CCGG[CT]G
SspI         1810  AATATT               AAT^ATT
Van91I       2403  CCAGACGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

AflIII       2895  ACATGT               A^C[GA][CT]GT
             3113  ACATGT              
Alw44I        887  GTGCAC               G^TGCAC
             1922  GTGCAC              
ApaLI         887  GTGCAC               G^TGCAC
             1922  GTGCAC              
AspI         1963  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3556  GACACGGTC           
BanI          142  GGTACC               G^G[CT][GA]CC
             1588  GGTGCC              
BbsI         1627  GAAGAC               GAAGAC(2/6)
             3124  GAAGAC              
BcgI         2594  GCATTGACTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3200  GCAAGTTGTTCG        
BclI          659  TGATCA               T^GATCA
             2823  TGATCA              
BpuAI        1627  GAAGAC               GAAGAC(2/6)
             3124  GAAGAC              
BsaI          718  GAGACC               GGTCTC(1/5)
             1409  GGTCTC              
BsaWI         602  TCCGGT               [AT]^CCGG[AT]
             3737  TCCGGA              
BsmI          633  GAATGC               GAATGC(1/-1)
             3048  GAATGC              
Bsp1407I     2789  TGTACA               T^GTACA
             2890  TGTACA              
BspLU11I     2895  ACATGT               A^CATGT
             3113  ACATGT              
BsrDI         366  CATTGC               GCAATG(2/0)
             1737  CATTGC              
BsrGI        2789  TGTACA               T^GTACA
             2890  TGTACA              
BstYI        1181  GGATCT               [GA]^GATC[CT]
             1496  GGATCT              
DrdI          211  GACTCCCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1933  GACCAAGGTGTC        
FbaI          659  TGATCA               T^GATCA
             2823  TGATCA              
HaeII         169  AGCGCC               [GA]GCGC^[CT]
             1337  AGCGCC              
HgaI         2406  GACGC                GACGC(5/10)
             1364  GCGTC               
HincII        481  GTCAAC               GT[CT]^[GA]AC
             2083  GTTGAC              
HindII        481  GTCAAC               GT[CT]^[GA]AC
             2083  GTTGAC              
MflI         1181  GGATCT               [GA]^GATC[CT]
             1496  GGATCT              
NcoI         1646  CCATGG               C^CATGG
             2757  CCATGG              
PflFI        1963  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3556  GACACGGTC           
PpuMI         515  AGGTCCC              [GA]G^G[AT]CC[CT]
             2762  GGGACCT             
PvuII        1975  CAGCTG               CAG^CTG
             2520  CAGCTG              
SacI         1317  GAGCTC               GAGCT^C
             1567  GAGCTC              
SapI         2626  GCTCTTC              GCTCTTC(1/4)
             3282  GCTCTTC             
ScaI         2861  AGTACT               AGT^ACT
             3683  AGTACT              
SmaI          173  CCCGGG               CCC^GGG
             3419  CCCGGG              
SmlI          280  CTTGAG               C^T[CT][GA]AG
              625  CTTGAG              
SspBI        2789  TGTACA               T^GTACA
             2890  TGTACA              
Tth111I      1963  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3556  GACACGGTC           
XhoII        1181  GGATCT               [GA]^GATC[CT]
             1496  GGATCT              
XmaCI         173  CCCGGG               C^CCGGG
             3419  CCCGGG              
XmaI          173  CCCGGG               C^CCGGG
             3419  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaI        AvaII       BalI        BanII       BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrBI       BsrFI       BsrI        BssKI       BssSI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SphI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XcmI        
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