Restriction map for pF1KA0882
 (Linear) (Six-base) MAPSORT of: KIAA0882.seq  Check: 8854  from: 1  to: 3798



KIAA0882.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:21  ..

AccI GT'mk_AC

Cuts at:      0     221     611    2303    3798 
   Size:        221     390    1692    1495

  Fragments arranged by size: 

                1692    1495     390     221
 
Acc65I G'GTAC_C

Cuts at:      0     142    3798 
   Size:        142    3656
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1805    3798 
   Size:       1805    1993
 
AflIII A'CryG_T

Cuts at:      0    2895    3113    3798 
   Size:       2895     218     685

  Fragments arranged by size: 

                2895     685     218
 
AleI CACnn'nnGTG

Cuts at:      0     340    1822    3798 
   Size:        340    1482    1976

  Fragments arranged by size: 

                1976    1482     340
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1386    2391    2409    2842    3144    3648    3798 
   Size:       1386    1005      18     433     302     504     150

  Fragments arranged by size: 

                1386    1005     504     433     302     150      18
 
ApaLI G'TGCA_C

Cuts at:      0     887    1922    3798 
   Size:        887    1035    1876

  Fragments arranged by size: 

                1876    1035     887
 
ApoI r'AATT_y

Cuts at:      0     439    2338    2985    3798 
   Size:        439    1899     647     813

  Fragments arranged by size: 

                1899     813     647     439
 
AvaI C'yCGr_G

Cuts at:      0     173    1414    3419    3798 
   Size:        173    1241    2005     379

  Fragments arranged by size: 

                2005    1241     379     173
 
BanI G'GyrC_C

Cuts at:      0     142    1588    3798 
   Size:        142    1446    2210

  Fragments arranged by size: 

                2210    1446     142
 
BanII G_rGCy'C

Cuts at:      0    1321    1571    3335    3798 
   Size:       1321     250    1764     463

  Fragments arranged by size: 

                1764    1321     463     250
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1634    3131    3798 
   Size:       1634    1497     667

  Fragments arranged by size: 

                1634    1497     667
 
BbvCI CC'TCA_GC

Cuts at:      0     541    3798 
   Size:        541    3257
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2583    2617    3189    3223    3798 
   Size:       2583      34     572      34     575

  Fragments arranged by size: 

                2583     575     572      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2409    3798 
   Size:       2409    1389
 
BclI T'GATC_A

Cuts at:      0     659    2823    3798 
   Size:        659    2164     975

  Fragments arranged by size: 

                2164     975     659
 
BglI GCCn_nnn'nGGC

Cuts at:      0      98    3266    3349    3798 
   Size:         98    3168      83     449

  Fragments arranged by size: 

                3168     449      98      83
 
BlpI GC'TnA_GC

Cuts at:      0    2272    3798 
   Size:       2272    1526
 
Bme1580I G_kGCm'C

Cuts at:      0     891    1926    3586    3798 
   Size:        891    1035    1660     212

  Fragments arranged by size: 

                1660    1035     891     212
 
BmgBI CAC'GTC

Cuts at:      0    2802    3798 
   Size:       2802     996
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     279    1094    2538    3633    3798 
   Size:        279     815    1444    1095     165

  Fragments arranged by size: 

                1444    1095     815     279     165
 
BmtI G_CTAG'C

Cuts at:      0    1957    3798 
   Size:       1957    1841
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1287    2751    2835    3387    3429    3798 
   Size:       1287    1464      84     552      42     369

  Fragments arranged by size: 

                1464    1287     552     369      84      42
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150     191    1071    1281    2431    3008    3202    3424 
   Size:        150      41     880     210    1150     577     194     222

Cuts at:   3424    3798 
   Size:        374

  Fragments arranged by size: 

                1150     880     577     374     222     210     194     150
                  41
 
Bpu10I CC'TnA_GC

Cuts at:      0     541    2810    3798 
   Size:        541    2269     988

  Fragments arranged by size: 

                2269     988     541
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     301     646    3798 
   Size:        301     345    3152

  Fragments arranged by size: 

                3152     345     301
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     712    1415    3798 
   Size:        712     703    2383

  Fragments arranged by size: 

                2383     712     703
 
BsaAI yAC'GTr

Cuts at:      0    3315    3798 
   Size:       3315     483
 
BsaWI w'CCGG_w

Cuts at:      0     602    3737    3798 
   Size:        602    3135      61

  Fragments arranged by size: 

                3135     602      61
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       7      37    1519    1549    2229    2248    2259    2278 
   Size:          7      30    1482      30     680      19      11      19

Cuts at:   2278    3463    3493    3798 
   Size:       1185      30     305

  Fragments arranged by size: 

                1482    1185     680     305      30      30      30      19
                  19      11       7
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      31    1036    1284    1432    1687    1861    1963    3402 
   Size:         31    1005     248     148     255     174     102    1439

Cuts at:   3402    3429    3535    3798 
   Size:         27     106     263

  Fragments arranged by size: 

                1439    1005     263     255     248     174     148     106
                 102      31      27
 
BseYI C'CCAG_C

Cuts at:      0    1279    1713    2566    3215    3798 
   Size:       1279     434     853     649     583

  Fragments arranged by size: 

                1279     853     649     583     434
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     921    1195    1727    2090    2951    3517    3798 
   Size:        921     274     532     363     861     566     281

  Fragments arranged by size: 

                 921     861     566     532     363     281     274
 
BsiHKAI G_wGCw'C

Cuts at:      0     673     891    1097    1321    1571    1926    3284    3607 
   Size:        673     218     206     224     250     355    1358     323

Cuts at:   3607    3798 
   Size:        191

  Fragments arranged by size: 

                1358     673     355     323     250     224     218     206
                 191
 
BsmI GAATG_Cn'

Cuts at:      0     639    3054    3798 
   Size:        639    2415     744

  Fragments arranged by size: 

                2415     744     639
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1296    3798 
   Size:       1296    2502
 
Bsp1286I G_dGCh'C

Cuts at:      0     673     891    1097    1321    1571    1926    3284    3335 
   Size:        673     218     206     224     250     355    1358      51

Cuts at:   3335    3586    3607    3798 
   Size:        251      21     191

  Fragments arranged by size: 

                1358     673     355     251     250     224     218     206
                 191      51      21
 
BspEI T'CCGG_A

Cuts at:      0    3737    3798 
   Size:       3737      61
 
BsrBI CCG'CTC

Cuts at:      0    1050    1697    3239    3575    3798 
   Size:       1050     647    1542     336     223

  Fragments arranged by size: 

                1542    1050     647     336     223
 
BsrDI GCAATG_nn'

Cuts at:      0     365    1736    3798 
   Size:        365    1371    2062

  Fragments arranged by size: 

                2062    1371     365
 
BsrFI r'CCGG_y

Cuts at:      0      97    2555    3339    3508    3798 
   Size:         97    2458     784     169     290

  Fragments arranged by size: 

                2458     784     290     169      97
 
BsrGI T'GTAC_A

Cuts at:      0    2789    2890    3798 
   Size:       2789     101     908

  Fragments arranged by size: 

                2789     908     101
 
BssSI C'ACGA_G

Cuts at:      0    1314    1958    3517    3798 
   Size:       1314     644    1559     281

  Fragments arranged by size: 

                1559    1314     644     281
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2805    3798 
   Size:       2805     993
 
BstBI TT'CG_AA

Cuts at:      0    1776    3798 
   Size:       1776    2022
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1917    3798 
   Size:       1917    1881
 
BstYI r'GATC_y

Cuts at:      0    1181    1496    3798 
   Size:       1181     315    2302

  Fragments arranged by size: 

                2302    1181     315
 
Bsu36I CC'TnA_GG

Cuts at:      0    2297    3798 
   Size:       2297    1501
 
BtgI C'CryG_G

Cuts at:      0    1646    1667    2757    3155    3798 
   Size:       1646      21    1090     398     643

  Fragments arranged by size: 

                1646    1090     643     398      21
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     116     216    2291    3798 
   Size:        116     100    2075    1507

  Fragments arranged by size: 

                2075    1507     116     100
 
BtsI GCAGTG_nn'

Cuts at:      0    2239    3170    3252    3534    3592    3798 
   Size:       2239     931      82     282      58     206

  Fragments arranged by size: 

                2239     931     282     206      82      58
 
ClaI AT'CG_AT

Cuts at:      0     795    3798 
   Size:        795    3003
 
DraI TTT'AAA

Cuts at:      0     423    3798 
   Size:        423    3375
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     217    1939    3798 
   Size:        217    1722    1859

  Fragments arranged by size: 

                1859    1722     217
 
EaeI y'GGCC_r

Cuts at:      0      29     924    1436    3341    3576    3710    3764    3785 
   Size:         29     895     512    1905     235     134      54      21

Cuts at:   3785    3798 
   Size:         13

  Fragments arranged by size: 

                1905     895     512     235     134      54      29      21
                  13
 
EarI CTCTTCn'nnn_

Cuts at:      0     464    2633    2971    3289    3798 
   Size:        464    2169     338     318     509

  Fragments arranged by size: 

                2169     509     464     338     318
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     134    3798 
   Size:        134    3664
 
EcoICRI GAG'CTC

Cuts at:      0    1319    1569    3798 
   Size:       1319     250    2229

  Fragments arranged by size: 

                2229    1319     250
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150     191    1071    1281    1805    2431    3008    3202 
   Size:        150      41     880     210     524     626     577     194

Cuts at:   3202    3424    3798 
   Size:        222     374

  Fragments arranged by size: 

                 880     626     577     524     374     222     210     194
                 150      41
 
EcoO109I rG'GnC_Cy

Cuts at:      0     516    2763    3431    3798 
   Size:        516    2247     668     367

  Fragments arranged by size: 

                2247     668     516     367
 
EcoRV GAT'ATC

Cuts at:      0     330    3798 
   Size:        330    3468
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1491    3798 
   Size:       1491    2307
 
HaeII r_GCGC'y

Cuts at:      0     173    1341    3798 
   Size:        173    1168    2457

  Fragments arranged by size: 

                2457    1168     173
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1383    1415    1952    1984    3463    3495    3798 
   Size:       1383      32     537      32    1479      32     303

  Fragments arranged by size: 

                1479    1383     537     303      32      32      32
 
HincII GTy'rAC

Cuts at:      0     483    2085    3798 
   Size:        483    1602    1713

  Fragments arranged by size: 

                1713    1602     483
 
HindIII A'AGCT_T

Cuts at:      0    1853    3798 
   Size:       1853    1945
 
KpnI G_GTAC'C

Cuts at:      0     146    3798 
   Size:        146    3652
 
MfeI C'AATT_G

Cuts at:      0    1064    3798 
   Size:       1064    2734
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     511    2883    3798 
   Size:        140     371    2372     915

  Fragments arranged by size: 

                2372     915     371     140
 
MscI TGG'CCA

Cuts at:      0      31     926    1438    3712    3798 
   Size:         31     895     512    2274      86

  Fragments arranged by size: 

                2274     895     512      86      31
 
MslI CAynn'nnrTG

Cuts at:      0     340     754    1822    3739    3798 
   Size:        340     414    1068    1917      59

  Fragments arranged by size: 

                1917    1068     414     340      59
 
MspA1I CmG'CkG

Cuts at:      0      81    1977    2522    3798 
   Size:         81    1896     545    1276

  Fragments arranged by size: 

                1896    1276     545      81
 
NaeI GCC'GGC

Cuts at:      0      99    3341    3510    3798 
   Size:         99    3242     169     288

  Fragments arranged by size: 

                3242     288     169      99
 
NcoI C'CATG_G

Cuts at:      0    1646    2757    3798 
   Size:       1646    1111    1041

  Fragments arranged by size: 

                1646    1111    1041
 
NgoMIV G'CCGG_C

Cuts at:      0      97    3339    3508    3798 
   Size:         97    3242     169     290

  Fragments arranged by size: 

                3242     290     169      97
 
NheI G'CTAG_C

Cuts at:      0    1953    3798 
   Size:       1953    1845
 
NspI r_CATG'y

Cuts at:      0    1506    1560    1890    1992    2648    2756    2899    3117 
   Size:       1506      54     330     102     656     108     143     218

Cuts at:   3117    3798 
   Size:        681

  Fragments arranged by size: 

                1506     681     656     330     218     143     108     102
                  54
 
PciI A'CATG_T

Cuts at:      0    2895    3113    3798 
   Size:       2895     218     685

  Fragments arranged by size: 

                2895     685     218
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2409    3798 
   Size:       2409    1389
 
PfoI T'CCnGG_A

Cuts at:      0     247    1547    2723    3798 
   Size:        247    1300    1176    1075

  Fragments arranged by size: 

                1300    1176    1075     247
 
PpuMI rG'GwC_Cy

Cuts at:      0     516    2763    3798 
   Size:        516    2247    1035

  Fragments arranged by size: 

                2247    1035     516
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    3100    3132    3798 
   Size:       3100      32     666

  Fragments arranged by size: 

                3100     666      32
 
PstI C_TGCA'G

Cuts at:      0      80    3798 
   Size:         80    3718
 
PvuII CAG'CTG

Cuts at:      0    1977    2522    3798 
   Size:       1977     545    1276

  Fragments arranged by size: 

                1977    1276     545
 
SacI G_AGCT'C

Cuts at:      0    1321    1571    3798 
   Size:       1321     250    2227

  Fragments arranged by size: 

                2227    1321     250
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2633    3289    3798 
   Size:       2633     656     509

  Fragments arranged by size: 

                2633     656     509
 
ScaI AGT'ACT

Cuts at:      0    2863    3685    3798 
   Size:       2863     822     113

  Fragments arranged by size: 

                2863     822     113
 
SfcI C'TryA_G

Cuts at:      0      76    3798 
   Size:         76    3722
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    3349    3798 
   Size:       3349     449
 
SgrAI Cr'CCGG_yG

Cuts at:      0      97    3798 
   Size:         97    3701
 
SmaI CCC'GGG

Cuts at:      0     175    3421    3798 
   Size:        175    3246     377

  Fragments arranged by size: 

                3246     377     175
 
SmlI C'TyrA_G

Cuts at:      0     280     625    3798 
   Size:        280     345    3173

  Fragments arranged by size: 

                3173     345     280
 
SphI G_CATG'C

Cuts at:      0    1560    1890    1992    2756    3798 
   Size:       1560     330     102     764    1042

  Fragments arranged by size: 

                1560    1042     764     330     102
 
SspI AAT'ATT

Cuts at:      0    1812    3798 
   Size:       1812    1986
 
StyI C'CwwG_G

Cuts at:      0    1646    1935    2638    2757    3375    3467    3798 
   Size:       1646     289     703     119     618      92     331

  Fragments arranged by size: 

                1646     703     618     331     289     119      92
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1727    1909    3798 
   Size:       1727     182    1889

  Fragments arranged by size: 

                1889    1727     182
 
TatI w'GTAC_w

Cuts at:      0    1268    2789    2861    2890    3683    3798 
   Size:       1268    1521      72      29     793     115

  Fragments arranged by size: 

                1521    1268     793     115      72      29
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2096    2128    2229    2248    2261    2280    3708    3740 
   Size:       2096      32     101      19      13      19    1428      32

Cuts at:   3740    3798 
   Size:         58

  Fragments arranged by size: 

                2096    1428     101      58      32      32      19      19
                  13
 
Tth111I GACn'n_nGTC

Cuts at:      0    1966    3559    3798 
   Size:       1966    1593     239

  Fragments arranged by size: 

                1966    1593     239
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     100    2196    2475    3177    3798 
   Size:        100    2096     279     702     621

  Fragments arranged by size: 

                2096     702     621     279     100
 
XmaI C'CCGG_G

Cuts at:      0     173    3419    3798 
   Size:        173    3246     379

  Fragments arranged by size: 

                3246     379     173
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI   AflIII     AleI    AlwNI    ApaLI     ApoI
     AvaI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI     BclI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRV     FalI    HaeII    Hin4I
   HincII  HindIII     KpnI     MfeI     MmeI     MscI     MslI   MspA1I
     NaeI     NcoI   NgoMIV     NheI     NspI     PciI    PflMI     PfoI
    PpuMI     PsrI     PstI    PvuII     SacI     SapI     ScaI     SfcI
     SfiI    SgrAI     SmaI     SmlI     SphI     SspI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII     AgeI     AhdI     AlfI
     AloI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI    BfrBI    BglII    BsaBI    BsaHI    BsiEI    BsiWI    BspHI
    BspMI   BssHII   BstEII  BstZ17I    CspCI   DraIII     EagI    EcoNI
    EcoRI     FseI     FspI    FspAI     HpaI     KasI     MluI     NarI
     NdeI     NotI     NruI     NsiI     PacI     PasI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PvuI    RsrII    SacII
     SalI    SanDI     SbfI    SexAI     SfoI     SgfI    SnaBI     SpeI
     SrfI     StuI     SwaI     XbaI     XhoI     XmnI     ZraI
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