Restriction map for pF1KA0866
 (Linear) (Six-base) MAPSORT of: KIAA0866.seq  Check: 5342  from: 1  to: 5916



KIAA0866.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     398    2526    3768    5559    5916 
   Size:        398    2128    1242    1791     357

  Fragments arranged by size: 

                2128    1791    1242     398     357
 
AatII G_ACGT'C

Cuts at:      0     755    5916 
   Size:        755    5161
 
AccI GT'mk_AC

Cuts at:      0     416    4003    5916 
   Size:        416    3587    1913

  Fragments arranged by size: 

                3587    1913     416
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3349    3556    4258    4876    5551    5572    5916 
   Size:       3349     207     702     618     675      21     344

  Fragments arranged by size: 

                3349     702     675     618     344     207      21
 
AflII C'TTAA_G

Cuts at:      0    2623    5916 
   Size:       2623    3293
 
AflIII A'CryG_T

Cuts at:      0     419    3998    5916 
   Size:        419    3579    1918

  Fragments arranged by size: 

                3579    1918     419
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1107    3930    5916 
   Size:       1107    2823    1986

  Fragments arranged by size: 

                2823    1986    1107
 
AleI CACnn'nnGTG

Cuts at:      0    1315    2518    5916 
   Size:       1315    1203    3398

  Fragments arranged by size: 

                3398    1315    1203
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2125    4163    5722    5916 
   Size:       2125    2038    1559     194

  Fragments arranged by size: 

                2125    2038    1559     194
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     697     729    1603    1635    2990    3022    5916 
   Size:        697      32     874      32    1355      32    2894

  Fragments arranged by size: 

                2894    1355     874     697      32      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     582    2436    2716    2757    2856    2904    3156    3684 
   Size:        582    1854     280      41      99      48     252     528

Cuts at:   3684    3789    3966    4083    4284    5916 
   Size:        105     177     117     201    1632

  Fragments arranged by size: 

                1854    1632     582     528     280     252     201     177
                 117     105      99      48      41
 
ApaI G_GGCC'C

Cuts at:      0    3822    4473    4608    5916 
   Size:       3822     651     135    1308

  Fragments arranged by size: 

                3822    1308     651     135
 
ApaLI G'TGCA_C

Cuts at:      0    1659    5916 
   Size:       1659    4257
 
ApoI r'AATT_y

Cuts at:      0     730    4354    5916 
   Size:        730    3624    1562

  Fragments arranged by size: 

                3624    1562     730
 
AseI AT'TA_AT

Cuts at:      0    1160    5916 
   Size:       1160    4756
 
AvaI C'yCGr_G

Cuts at:      0    3821    4466    4504    4745    5122    5368    5879    5916 
   Size:       3821     645      38     241     377     246     511      37

  Fragments arranged by size: 

                3821     645     511     377     246     241      38      37
            
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     609     642    5916 
   Size:        609      33    5274

  Fragments arranged by size: 

                5274     609      33
 
BamHI G'GATC_C

Cuts at:      0    1367    5916 
   Size:       1367    4549
 
BanII G_rGCy'C

Cuts at:      0    1562    1912    3242    3503    3623    3822    3833    4473 
   Size:       1562     350    1330     261     120     199      11     640

Cuts at:   4473    4608    5106    5390    5916 
   Size:        135     498     284     526

  Fragments arranged by size: 

                1562    1330     640     526     498     350     284     261
                 199     135     120      11
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1071    2151    2593    2810    3473    4547    4639    4823 
   Size:       1071    1080     442     217     663    1074      92     184

Cuts at:   4823    5916 
   Size:       1093

  Fragments arranged by size: 

                1093    1080    1074    1071     663     442     217     184
                  92
 
BbvCI CC'TCA_GC

Cuts at:      0    2717    2927    4421    5916 
   Size:       2717     210    1494    1495

  Fragments arranged by size: 

                2717    1495    1494     210
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     729     763    5916 
   Size:        729      34    5153

  Fragments arranged by size: 

                5153     729      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1298    5916 
   Size:       1298    4618
 
BclI T'GATC_A

Cuts at:      0    2249    2973    4359    5916 
   Size:       2249     724    1386    1557

  Fragments arranged by size: 

                2249    1557    1386     724
 
BglI GCCn_nnn'nGGC

Cuts at:      0     592    2138    2380    2410    5916 
   Size:        592    1546     242      30    3506

  Fragments arranged by size: 

                3506    1546     592     242      30
 
BglII A'GATC_T

Cuts at:      0    1391    5009    5916 
   Size:       1391    3618     907

  Fragments arranged by size: 

                3618    1391     907
 
BlpI GC'TnA_GC

Cuts at:      0    5305    5916 
   Size:       5305     611
 
Bme1580I G_kGCm'C

Cuts at:      0     947    1663    3822    4473    4608    4839    5358    5916 
   Size:        947     716    2159     651     135     231     519     558

  Fragments arranged by size: 

                2159     947     716     651     558     519     231     135
            
 
BmgBI CAC'GTC

Cuts at:      0     281    1863    3927    4566    5916 
   Size:        281    1582    2064     639    1350

  Fragments arranged by size: 

                2064    1582    1350     639     281
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      64      96    5916 
   Size:         64      32    5820

  Fragments arranged by size: 

                5820      64      32
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1422    5916 
   Size:       1422    4494
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     560     694     887    1501    1568    2107    2779    2812 
   Size:        560     134     193     614      67     539     672      33

Cuts at:   2812    2896    2968    3178    3199    3205    3352    3367    3463 
   Size:         84      72     210      21       6     147      15      96

Cuts at:   3463    3706    3790    3934    4042    4105    4609    4630    4672 
   Size:        243      84     144     108      63     504      21      42

Cuts at:   4672    4906    4983    5164    5221    5242    5254    5476    5677 
   Size:        234      77     181      57      21      12     222     201

Cuts at:   5677    5846    5916 
   Size:        169      70

  Fragments arranged by size: 

                 672     614     560     539     504     243     234     222
                 210     201     193     181     169     147     144     134
                 108      96      84      84      77      72      70      67
                  63      57      42      33      21      21      21      15
                  12       6
 
Bpu10I CC'TnA_GC

Cuts at:      0    2717    2927    3502    3575    4421    5782    5916 
   Size:       2717     210     575      73     846    1361     134

  Fragments arranged by size: 

                2717    1361     846     575     210     134      73
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1690    2464    2932    3031    3226    3655    4699    5629 
   Size:       1690     774     468      99     195     429    1044     930

Cuts at:   5629    5755    5916 
   Size:        126     161

  Fragments arranged by size: 

                1690    1044     930     774     468     429     195     161
                 126      99
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     778    3419    4606    5791    5916 
   Size:        778    2641    1187    1185     125

  Fragments arranged by size: 

                2641    1187    1185     778     125
 
BsaAI yAC'GTr

Cuts at:      0     347    5916 
   Size:        347    5569
 
BsaHI Gr'CG_yC

Cuts at:      0     752    1108    2318    3931    4679    5916 
   Size:        752     356    1210    1613     748    1237

  Fragments arranged by size: 

                1613    1237    1210     752     748     356
 
BsaWI w'CCGG_w

Cuts at:      0     485    2150    5390    5916 
   Size:        485    1665    3240     526

  Fragments arranged by size: 

                3240    1665     526     485
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     265     280     295     310     499     529     697     727 
   Size:        265      15      15      15     189      30     168      30

Cuts at:    727    1642    1672    2990    3020    3778    3808    5916 
   Size:        915      30    1318      30     758      30    2108

  Fragments arranged by size: 

                2108    1318     915     758     265     189     168      30
                  30      30      30      30      15      15      15
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     169     588     705    1033    1036    1184    1504    1818 
   Size:        169     419     117     328       3     148     320     314

Cuts at:   1818    2350    2353    2566    2569    2674    2734    2776    2809 
   Size:        532       3     213       3     105      60      42      33

Cuts at:   2809    2812    2893    2896    3028    3280    3283    3451    3631 
   Size:          3      81       3     132     252       3     168     180

Cuts at:   3631    4039    4042    4075    4240    4390    4639    4777    4785 
   Size:        408       3      33     165     150     249     138       8

Cuts at:   4785    5158    5239    5242    5251    5254    5494    5674    5683 
   Size:        373      81       3       9       3     240     180       9

Cuts at:   5683    5686    5878    5916 
   Size:          3     192      38

  Fragments arranged by size: 

                 532     419     408     373     328     320     314     252
                 249     240     213     192     180     180     169     168
                 165     150     148     138     132     117     105      81
                  81      60      42      38      33      33       9       9
                   8       3       3       3       3       3       3       3
                   3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     951    1741    1918    1980    2846    3944    4214    5216 
   Size:        951     790     177      62     866    1098     270    1002

Cuts at:   5216    5678    5916 
   Size:        462     238

  Fragments arranged by size: 

                1098    1002     951     866     790     462     270     238
                 177      62
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3802    3822    5916 
   Size:       3802      20    2094

  Fragments arranged by size: 

                3802    2094      20
 
BsiEI CG_ry'CG

Cuts at:      0    1573    4238    5271    5916 
   Size:       1573    2665    1033     645

  Fragments arranged by size: 

                2665    1573    1033     645
 
BsiHKAI G_wGCw'C

Cuts at:      0    1562    1663    1912    3242    3503    3623    3833    5390 
   Size:       1562     101     249    1330     261     120     210    1557

Cuts at:   5390    5916 
   Size:        526

  Fragments arranged by size: 

                1562    1557    1330     526     261     249     210     120
                 101
 
BsmI GAATG_Cn'

Cuts at:      0    1618    2120    2205    2240    5916 
   Size:       1618     502      85      35    3676

  Fragments arranged by size: 

                3676    1618     502      85      35
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     821    3550    4798    5876    5916 
   Size:        821    2729    1248    1078      40

  Fragments arranged by size: 

                2729    1248    1078     821      40
 
Bsp1286I G_dGCh'C

Cuts at:      0     947    1562    1663    1912    3242    3503    3623    3822 
   Size:        947     615     101     249    1330     261     120     199

Cuts at:   3822    3833    4473    4608    4839    5106    5358    5390    5916 
   Size:         11     640     135     231     267     252      32     526

  Fragments arranged by size: 

                1330     947     640     615     526     267     261     252
                 249     231     199     135     120     101      32      11
            
 
BspEI T'CCGG_A

Cuts at:      0    5390    5916 
   Size:       5390     526
 
BspHI T'CATG_A

Cuts at:      0    2246    5916 
   Size:       2246    3670
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     398    2526    2678    3768    5475    5559    5916 
   Size:        398    2128     152    1090    1707      84     357

  Fragments arranged by size: 

                2128    1707    1090     398     357     152      84
 
BsrBI CCG'CTC

Cuts at:      0     321    2598    3390    3816    4032    4509    5373    5916 
   Size:        321    2277     792     426     216     477     864     543

  Fragments arranged by size: 

                2277     864     792     543     477     426     321     216
            
 
BsrDI GCAATG_nn'

Cuts at:      0    2109    5631    5916 
   Size:       2109    3522     285

  Fragments arranged by size: 

                3522    2109     285
 
BsrFI r'CCGG_y

Cuts at:      0    2132    5916 
   Size:       2132    3784
 
BsrGI T'GTAC_A

Cuts at:      0     422    1967    5916 
   Size:        422    1545    3949

  Fragments arranged by size: 

                3949    1545     422
 
BssSI C'ACGA_G

Cuts at:      0     442    2047    3202    4324    4795    5404    5916 
   Size:        442    1605    1155    1122     471     609     512

  Fragments arranged by size: 

                1605    1155    1122     609     512     471     442
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     447    2904    3789    5916 
   Size:        447    2457     885    2127

  Fragments arranged by size: 

                2457    2127     885     447
 
BstBI TT'CG_AA

Cuts at:      0    4727    5916 
   Size:       4727    1189
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1741    1785    3915    3927    4158    5052    5916 
   Size:       1741      44    2130      12     231     894     864

  Fragments arranged by size: 

                2130    1741     894     864     231      44      12
 
BstYI r'GATC_y

Cuts at:      0    1180    1367    1391    2186    2957    3335    4734    5009 
   Size:       1180     187      24     795     771     378    1399     275

Cuts at:   5009    5916 
   Size:        907

  Fragments arranged by size: 

                1399    1180     907     795     771     378     275     187
                  24
 
Bsu36I CC'TnA_GG

Cuts at:      0     313    5916 
   Size:        313    5603
 
BtgI C'CryG_G

Cuts at:      0     587     986     995    1631    4157    4670    5729    5747 
   Size:        587     399       9     636    2526     513    1059      18

Cuts at:   5747    5916 
   Size:        169

  Fragments arranged by size: 

                2526    1059     636     587     513     399     169      18
                   9
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     453    2398    3821    5430    5916 
   Size:        453    1945    1423    1609     486

  Fragments arranged by size: 

                1945    1609    1423     486     453
 
BtsI GCAGTG_nn'

Cuts at:      0    2503    2527    5916 
   Size:       2503      24    3389

  Fragments arranged by size: 

                3389    2503      24
 
EaeI y'GGCC_r

Cuts at:      0     584    1271    1844    1892    2402    2741    3254    3287 
   Size:        584     687     573      48     510     339     513      33

Cuts at:   3287    3707    3917    4235    5150    5318    5516    5591    5916 
   Size:        420     210     318     915     168     198      75     325

  Fragments arranged by size: 

                 915     687     584     573     513     510     420     339
                 325     318     210     198     168      75      48      33
            
 
EagI C'GGCC_G

Cuts at:      0    4235    5916 
   Size:       4235    1681
 
EarI CTCTTCn'nnn_

Cuts at:      0     364     430    1462    2047    3044    3131    3136    3548 
   Size:        364      66    1032     585     997      87       5     412

Cuts at:   3548    4166    4177    4395    4472    4640    4762    5916 
   Size:        618      11     218      77     168     122    1154

  Fragments arranged by size: 

                1154    1032     997     618     585     412     364     218
                 168     122      87      77      66      11       5
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     286    2161    3841    5916 
   Size:        286    1875    1680    2075

  Fragments arranged by size: 

                2075    1875    1680     286
 
EcoICRI GAG'CTC

Cuts at:      0    1560    1910    3240    3501    3621    3831    5388    5916 
   Size:       1560     350    1330     261     120     210    1557     528

  Fragments arranged by size: 

                1560    1557    1330     528     350     261     210     120
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     560     694     887    1501    1568    2107    2779    2812 
   Size:        560     134     193     614      67     539     672      33

Cuts at:   2812    2896    2968    3178    3199    3205    3349    3352    3367 
   Size:         84      72     210      21       6     144       3      15

Cuts at:   3367    3463    3556    3706    3790    3934    4042    4105    4258 
   Size:         96      93     150      84     144     108      63     153

Cuts at:   4258    4609    4630    4672    4876    4906    4983    5164    5221 
   Size:        351      21      42     204      30      77     181      57

Cuts at:   5221    5242    5254    5476    5551    5572    5677    5846    5916 
   Size:         21      12     222      75      21     105     169      70

  Fragments arranged by size: 

                 672     614     560     539     351     222     210     204
                 193     181     169     153     150     144     144     134
                 108     105      96      93      84      84      77      75
                  72      70      67      63      57      42      33      30
                  21      21      21      21      15      12       6       3
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1483    1855    2335    2674    2794    3370    3433    3730 
   Size:       1483     372     480     339     120     576      63     297

Cuts at:   3730    4477    5125    5916 
   Size:        747     648     791

  Fragments arranged by size: 

                1483     791     747     648     576     480     372     339
                 297     120      63
 
EcoO109I rG'GnC_Cy

Cuts at:      0     111    3375    3447    3543    3723    4452    4470    4605 
   Size:        111    3264      72      96     180     729      18     135

Cuts at:   4605    4872    5082    5916 
   Size:        267     210     834

  Fragments arranged by size: 

                3264     834     729     267     210     180     135     111
                  96      72      18
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      57     147    5577    5916 
   Size:         57      90    5430     339

  Fragments arranged by size: 

                5430     339      90      57
 
HaeII r_GCGC'y

Cuts at:      0    1293    1925    4092    4714    5196    5455    5916 
   Size:       1293     632    2167     622     482     259     461

  Fragments arranged by size: 

                2167    1293     632     622     482     461     259
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     438     470     499     531     700     732    1015    1047 
   Size:        438      32      29      32     169      32     283      32

Cuts at:   1047    1093    1125    1172    1204    1376    1408    2041    2073 
   Size:         46      32      47      32     172      32     633      32

Cuts at:   2073    3367    3399    3547    3579    3778    3810    3916    3948 
   Size:       1294      32     148      32     199      32     106      32

Cuts at:   3948    4994    5026    5659    5691    5916 
   Size:       1046      32     633      32     225

  Fragments arranged by size: 

                1294    1046     633     633     438     283     225     199
                 172     169     148     106      47      46      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      29
 
HincII GTy'rAC

Cuts at:      0     375     417    4701    5916 
   Size:        375      42    4284    1215

  Fragments arranged by size: 

                4284    1215     375      42
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1856    4145    4355    5916 
   Size:       1856    2289     210    1561

  Fragments arranged by size: 

                2289    1856    1561     210
 
MscI TGG'CCA

Cuts at:      0    1273    1894    2404    3256    3289    3919    5152    5320 
   Size:       1273     621     510     852      33     630    1233     168

Cuts at:   5320    5916 
   Size:        596

  Fragments arranged by size: 

                1273    1233     852     630     621     596     510     168
                  33
 
MslI CAynn'nnrTG

Cuts at:      0    1315    2245    2449    2518    3880    4799    5916 
   Size:       1315     930     204      69    1362     919    1117

  Fragments arranged by size: 

                1362    1315    1117     930     919     204      69
 
MspA1I CmG'CkG

Cuts at:      0    1129    1301    1419    1794    1965    1980    2094    2436 
   Size:       1129     172     118     375     171      15     114     342

Cuts at:   2436    2652    2685    2874    3252    4020    4053    4317    4641 
   Size:        216      33     189     378     768      33     264     324

Cuts at:   4641    4846    5095    5220    5655    5712    5916 
   Size:        205     249     125     435      57     204

  Fragments arranged by size: 

                1129     768     435     378     375     342     324     264
                 249     216     205     204     189     172     171     125
                 118     114      57      33      33      15
 
NaeI GCC'GGC

Cuts at:      0    2134    5916 
   Size:       2134    3782
 
NcoI C'CATG_G

Cuts at:      0     995    5747    5916 
   Size:        995    4752     169

  Fragments arranged by size: 

                4752     995     169
 
NdeI CA'TA_TG

Cuts at:      0    1284    5916 
   Size:       1284    4632
 
NgoMIV G'CCGG_C

Cuts at:      0    2132    5916 
   Size:       2132    3784
 
NotI GC'GGCC_GC

Cuts at:      0    4235    5916 
   Size:       4235    1681
 
NspI r_CATG'y

Cuts at:      0     423     495    1947    4707    5916 
   Size:        423      72    1452    2760    1209

  Fragments arranged by size: 

                2760    1452    1209     423      72
 
PciI A'CATG_T

Cuts at:      0     419    5916 
   Size:        419    5497
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1152    4587    5409    5916 
   Size:       1152    3435     822     507

  Fragments arranged by size: 

                3435    1152     822     507
 
PfoI T'CCnGG_A

Cuts at:      0     977    1370    1478    2162    3338    3368    3581    3950 
   Size:        977     393     108     684    1176      30     213     369

Cuts at:   3950    5180    5916 
   Size:       1230     736

  Fragments arranged by size: 

                1230    1176     977     736     684     393     369     213
                 108      30
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     697     729    2990    3022    5916 
   Size:        697      32    2261      32    2894

  Fragments arranged by size: 

                2894    2261     697      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     111    3375    3723    4872    5082    5916 
   Size:        111    3264     348    1149     210     834

  Fragments arranged by size: 

                3264    1149     834     348     210     111
 
PspOMI G'GGCC_C

Cuts at:      0    3818    4469    4604    5916 
   Size:       3818     651     135    1312

  Fragments arranged by size: 

                3818    1312     651     135
 
PspXI vC'TCGA_Gb

Cuts at:      0    4504    5368    5916 
   Size:       4504     864     548

  Fragments arranged by size: 

                4504     864     548
 
PstI C_TGCA'G

Cuts at:      0    1064    1451    2537    2585    2690    3278    3794    3857 
   Size:       1064     387    1086      48     105     588     516      63

Cuts at:   3857    4142    4784    4851    4952    5570    5711    5916 
   Size:        285     642      67     101     618     141     205

  Fragments arranged by size: 

                1086    1064     642     618     588     516     387     285
                 205     141     105     101      67      63      48
 
PvuII CAG'CTG

Cuts at:      0    1129    1419    1965    1980    2094    2436    2652    2685 
   Size:       1129     290     546      15     114     342     216      33

Cuts at:   2685    2874    3252    4020    4053    4641    4846    5220    5655 
   Size:        189     378     768      33     588     205     374     435

Cuts at:   5655    5916 
   Size:        261

  Fragments arranged by size: 

                1129     768     588     546     435     378     374     342
                 290     261     216     205     189     114      33      33
                  15
 
SacI G_AGCT'C

Cuts at:      0    1562    1912    3242    3503    3623    3833    5390    5916 
   Size:       1562     350    1330     261     120     210    1557     526

  Fragments arranged by size: 

                1562    1557    1330     526     350     261     210     120
            
 
SalI G'TCGA_C

Cuts at:      0     415    5916 
   Size:        415    5501
 
SanDI GG'GwC_CC

Cuts at:      0     111    5916 
   Size:        111    5805
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1462    3136    4640    5916 
   Size:       1462    1674    1504    1276

  Fragments arranged by size: 

                1674    1504    1462    1276
 
SexAI A'CCwGG_T

Cuts at:      0    4292    4544    5916 
   Size:       4292     252    1372

  Fragments arranged by size: 

                4292    1372     252
 
SfcI C'TryA_G

Cuts at:      0    1060    1259    1447    1543    2533    2581    2674    2686 
   Size:       1060     199     188      96     990      48      93      12

Cuts at:   2686    2821    3274    3790    3853    4138    4780    4847    4948 
   Size:        135     453     516      63     285     642      67     101

Cuts at:   4948    5566    5707    5916 
   Size:        618     141     209

  Fragments arranged by size: 

                1060     990     642     618     516     453     285     209
                 199     188     141     135     101      96      93      67
                  63      48      12
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     592    5916 
   Size:        592    5324
 
SmaI CCC'GGG

Cuts at:      0    3823    4747    5916 
   Size:       3823     924    1169

  Fragments arranged by size: 

                3823    1169     924
 
SmlI C'TyrA_G

Cuts at:      0    1705    2479    2623    2911    3010    3241    3634    4504 
   Size:       1705     774     144     288      99     231     393     870

Cuts at:   4504    4714    5122    5368    5644    5770    5879    5916 
   Size:        210     408     246     276     126     109      37

  Fragments arranged by size: 

                1705     870     774     408     393     288     276     246
                 231     210     144     126     109      99      37
 
SnaBI TAC'GTA

Cuts at:      0     347    5916 
   Size:        347    5569
 
SphI G_CATG'C

Cuts at:      0     495    5916 
   Size:        495    5421
 
SrfI GCCC'GGGC

Cuts at:      0    3823    5916 
   Size:       3823    2093
 
StuI AGG'CCT

Cuts at:      0    2236    4483    5916 
   Size:       2236    2247    1433

  Fragments arranged by size: 

                2247    2236    1433
 
StyI C'CwwG_G

Cuts at:      0     254     872     995    1274    2567    2591    3653    3920 
   Size:        254     618     123     279    1293      24    1062     267

Cuts at:   3920    4448    4484    4556    5747    5903    5916 
   Size:        528      36      72    1191     156      13

  Fragments arranged by size: 

                1293    1191    1062     618     528     279     267     254
                 156     123      72      36      24      13
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     258    1044    1587    2671    3973    4228    5916 
   Size:        258     786     543    1084    1302     255    1688

  Fragments arranged by size: 

                1688    1302    1084     786     543     258     255
 
TatI w'GTAC_w

Cuts at:      0     422    1967    5600    5916 
   Size:        422    1545    3633     316

  Fragments arranged by size: 

                3633    1545     422     316
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     265     297    1148    1180    1642    1674    5916 
   Size:        265      32     851      32     462      32    4242

  Fragments arranged by size: 

                4242     851     462     265      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    3561    3841    4546    5272    5916 
   Size:       3561     280     705     726     644

  Fragments arranged by size: 

                3561     726     705     644     280
 
XbaI T'CTAG_A

Cuts at:      0    5815    5916 
   Size:       5815     101
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1002    1041    1476    2217    3744    4080    4563    4605 
   Size:       1002      39     435     741    1527     336     483      42

Cuts at:   4605    4689    5451    5916 
   Size:         84     762     465

  Fragments arranged by size: 

                1527    1002     762     741     483     465     435     336
                  84      42      39
 
XhoI C'TCGA_G

Cuts at:      0    4504    5122    5368    5879    5916 
   Size:       4504     618     246     511      37

  Fragments arranged by size: 

                4504     618     511     246      37
 
XmaI C'CCGG_G

Cuts at:      0    3821    4745    5916 
   Size:       3821     924    1171

  Fragments arranged by size: 

                3821    1171     924
 
XmnI GAAnn'nnTTC

Cuts at:      0    1408    2120    4186    5916 
   Size:       1408     712    2066    1730

  Fragments arranged by size: 

                2066    1730    1408     712
 
ZraI GAC'GTC

Cuts at:      0     753    5916 
   Size:        753    5163
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI    AflII   AflIII     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI     AvaI
     BaeI    BamHI    BanII     BbsI    BbvCI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
    HaeII    Hin4I   HincII     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI     NdeI   NgoMIV     NotI     NspI     PciI    PflMI     PfoI
     PpiI    PpuMI   PspOMI    PspXI     PstI    PvuII     SacI     SalI
    SanDI     SapI    SexAI     SfcI     SfiI     SmaI     SmlI    SnaBI
     SphI     SrfI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI     AgeI     AscI    AsiSI    AvrII     BanI
     BbeI    BfrBI     BmtI    BsaBI    BsiWI   BssHII   BstEII  BstZ17I
     ClaI    CspCI     DraI   DraIII     DrdI    EcoRI    EcoRV     FseI
     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NarI     NheI     NruI     NsiI     PacI     PasI     PmeI     PmlI
    PshAI     PsiI     PsrI     PvuI    RsrII    SacII     SbfI     ScaI
     SfoI     SgfI    SgrAI     SpeI     SspI     SwaI
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