Restriction map for pF1KA0837
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AflII       AgeI        AhdI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbrPI       BbsI        
BcgI        BfrI        BlnI        BpuAI       BsaBI       
BseRI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspHI       BsrDI       BsrGI       
BssHII      Bst1107I    BstAPI      BstEII      BstPI       
BstUI       BstZ17I     ClaI        CpoI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco52I      
EcoO65I     EcoRI       EcoT22I     FseI        FspI        
HgaI        HincII      HindII      HindIII     HpaI        
KpnI        KspI        MamI        MluI        MvnI        
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SacII       SalI        SbfI        SfcI        SfiI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        Tth111I     XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AcsI          466  GAATTT               [GA]^AATT[CT]
AcyI         1518  GGCGCC               G[GA]^CG[CT]C
Alw44I       1461  GTGCAC               G^TGCAC
AlwNI         430  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1232  AGCGCT               AGC^GCT
ApaLI        1461  GTGCAC               G^TGCAC
ApoI          466  GAATTT               [GA]^AATT[CT]
AvaI         1783  CCCGAG               C^[CT]CG[GA]G
BbeI         1518  GGCGCC               GGCGC^C
BbvCI         310  CCTCAGC              CCTCAGC(-5/-2)
BciVI          98  GGATAC               GTATCC(6/5)
BglI          454  GCCGACAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          372  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I      372  GCTTAGC              GC^T[ACGT]AGC
BsaAI        1510  CACGTA               [CT]AC^GT[GA]
BsaHI        1518  GGCGCC               G[GA]^CG[CT]C
BsaI         2106  GAGACC               GGTCTC(1/5)
BsgI         1947  CTGCAC               GTGCAG(16/14)
BsoBI        1783  CCCGAG               C^[CT]CG[GA]G
BspMI        2028  GCAGGT               ACCTGC(4/8)
BsrBI         601  CCGCTC               CCGCTC(-3/-3)
BstBI         763  TTCGAA               TT^CGAA
BstXI         587  CCATGGTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         372  GCTTAGC              GC^T[ACGT]AGC
DraI         1753  TTTAAA               TTT^AAA
Eco47III     1232  AGCGCT               AGC^GCT
EcoNI         112  CCTGAGCTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        1114  GATATC               GAT^ATC
Hin1I        1518  GGCGCC               G[GA]^CG[CT]C
KasI         1518  GGCGCC               G^GCGCC
MfeI          495  CAATTG               C^AATTG
MunI          495  CAATTG               C^AATTG
NaeI         1710  GCCGGC               GCC^GGC
NarI         1518  GGCGCC               GG^CGCC
NdeI         1934  CATATG               CA^TATG
NgoMIV       1710  GCCGGC               G^CCGGC
NspV          763  TTCGAA               TT^CGAA
PvuII        1466  CAGCTG               CAG^CTG
SapI         2143  GAAGAGC              GCTCTTC(1/4)
ScaI         2123  AGTACT               AGT^ACT
SexAI         353  ACCAGGT              A^CC[AT]GGT
SfoI         1518  GGCGCC               GGC^GCC
SfuI          763  TTCGAA               TT^CGAA
SmlI          736  CTCAAG               C^T[CT][GA]AG
SphI          505  GCATGC               GCATG^C
TaqI          764  TCGA                 T^CGA
TthHB8I       764  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AflIII       1046  ACATGT               A^C[GA][CT]GT
             2057  ACATGT              
AocI           93  CCTGAGG              CC^T[ACGT]AGG
             1502  CCTCAGG             
AspHI        1018  GTGCTC               G[AT]GC[AT]^C
             1461  GTGCAC              
BalI          837  TGGCCA               TGG^CCA
             1449  TGGCCA              
BclI          513  TGATCA               T^GATCA
              743  TGATCA              
BcnI          341  CCCGG                CC^[GC]GG
             1413  CCGGG               
BglII        1202  AGATCT               A^GATCT
             1941  AGATCT              
BsiHKAI      1018  GTGCTC               G[AT]GC[AT]^C
             1461  GTGCAC              
BspLU11I     1046  ACATGT               A^CATGT
             2057  ACATGT              
BsrFI         212  ACCGGC               [GA]^CCGG[CT]
             1710  GCCGGC              
BssSI          16  CTCGTG               CACGAG(-5/-1)
              163  CTCGTG              
Bsu36I         93  CCTGAGG              CC^T[ACGT]AGG
             1502  CCTCAGG             
Cfr10I        212  ACCGGC               [GA]^CCGG[CT]
             1710  GCCGGC              
Eco81I         93  CCTGAGG              CC^T[ACGT]AGG
             1502  CCTCAGG             
FbaI          513  TGATCA               T^GATCA
              743  TGATCA              
HaeII        1232  AGCGCT               [GA]GCGC^[CT]
             1518  GGCGCC              
MaeII         950  ACGT                 A^CGT
             1511  ACGT                
MluNI         837  TGGCCA               TGG^CCA
             1449  TGGCCA              
MscI          837  TGGCCA               TGG^CCA
             1449  TGGCCA              
MspA1I        650  CAGCGG               C[AC]G^C[GT]G
             1466  CAGCTG              
NciI          341  CCCGG                CC^[GC]GG
             1413  CCGGG               
PflMI         444  CCAGGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1815  CCAACCTGTGG         
PleI          726  GACTC                GAGTC(4/5)
             2012  GACTC               
Tsp45I        122  GTGAC                ^GT[GC]AC
              982  GTGAC               
Van91I        444  CCAGGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1815  CCAACCTGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AccIII      AciI        AfaI        AluI        AlwI        
ApaI        AvaII       BanI        BanII       BbvI        
BfaI        BmyI        BpmI        BsaJI       BsaWI       
BseAI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspEI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MroI        MseI        MslI        
MspI        MvaI        MwoI        NcoI        NdeII       
NlaIII      NlaIV       NspI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       StuI        StyI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
XcmI        XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com