Restriction map for pF1KA0756
 (Linear) (Six-base) MAPSORT of: KIAA0756.seq  Check: 79  from: 1  to: 3507



KIAA0756.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     375    3012    3507 
   Size:        375    2637     495

  Fragments arranged by size: 

                2637     495     375
 
AccI GT'mk_AC

Cuts at:      0    2366    2534    3507 
   Size:       2366     168     973

  Fragments arranged by size: 

                2366     973     168
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1726    2498    2503    2623    3260    3507 
   Size:       1726     772       5     120     637     247

  Fragments arranged by size: 

                1726     772     637     247     120       5
 
AfeI AGC'GCT

Cuts at:      0    2143    3507 
   Size:       2143    1364
 
AflII C'TTAA_G

Cuts at:      0     711    3507 
   Size:        711    2796
 
AflIII A'CryG_T

Cuts at:      0     543     728    1802    1804    3507 
   Size:        543     185    1074       2    1703

  Fragments arranged by size: 

                1703    1074     543     185       2
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2985    3507 
   Size:       2985     522
 
AleI CACnn'nnGTG

Cuts at:      0    1645    3507 
   Size:       1645    1862
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1663    3507 
   Size:       1663    1844
 
ApaI G_GGCC'C

Cuts at:      0    2419    3507 
   Size:       2419    1088
 
ApoI r'AATT_y

Cuts at:      0      77     349    3507 
   Size:         77     272    3158

  Fragments arranged by size: 

                3158     272      77
 
AvaI C'yCGr_G

Cuts at:      0     245     740    1615    1880    1936    2167    2303    3507 
   Size:        245     495     875     265      56     231     136    1204

  Fragments arranged by size: 

                1204     875     495     265     245     231     136      56
            
 
AvrII C'CTAG_G

Cuts at:      0    2791    3507 
   Size:       2791     716
 
BamHI G'GATC_C

Cuts at:      0     374    1931    3507 
   Size:        374    1557    1576

  Fragments arranged by size: 

                1576    1557     374
 
BanI G'GyrC_C

Cuts at:      0     784    1170    1639    3325    3507 
   Size:        784     386     469    1686     182

  Fragments arranged by size: 

                1686     784     469     386     182
 
BanII G_rGCy'C

Cuts at:      0     527    2338    2419    3031    3507 
   Size:        527    1811      81     612     476

  Fragments arranged by size: 

                1811     612     527     476      81
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     965    1757    1994    3248    3404    3507 
   Size:        965     792     237    1254     156     103

  Fragments arranged by size: 

                1254     965     792     237     156     103
 
BbvCI CC'TCA_GC

Cuts at:      0    3007    3507 
   Size:       3007     500
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     785    2872    3507 
   Size:        785    2087     635

  Fragments arranged by size: 

                2087     785     635
 
BfrBI ATG'CAT

Cuts at:      0     836    3507 
   Size:        836    2671
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1875    2251    2663    3507 
   Size:       1875     376     412     844

  Fragments arranged by size: 

                1875     844     412     376
 
Bme1580I G_kGCm'C

Cuts at:      0     741     787    1642    2375    2419    2790    3507 
   Size:        741      46     855     733      44     371     717

  Fragments arranged by size: 

                 855     741     733     717     371      46      44
 
BmgBI CAC'GTC

Cuts at:      0    2187    3476    3507 
   Size:       2187    1289      31

  Fragments arranged by size: 

                2187    1289      31
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2356    2777    3199    3507 
   Size:       2356     421     422     308

  Fragments arranged by size: 

                2356     422     421     308
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     978    2318    3279    3507 
   Size:        978    1340     961     228

  Fragments arranged by size: 

                1340     978     961     228
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     445    1600    1675    1909    2604    2725    2842    3507 
   Size:        445    1155      75     234     695     121     117     665

  Fragments arranged by size: 

                1155     695     665     445     234     121     117      75
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    2420    2852    3007    3507 
   Size:       2420     432     155     500

  Fragments arranged by size: 

                2420     500     432     155
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     658    3507 
   Size:        658    2849
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     560    1406    2743    3507 
   Size:        560     846    1337     764

  Fragments arranged by size: 

                1337     846     764     560
 
BsaAI yAC'GTr

Cuts at:      0    1805    2228    2346    2370    3507 
   Size:       1805     423     118      24    1137

  Fragments arranged by size: 

                1805    1137     423     118      24
 
BsaHI Gr'CG_yC

Cuts at:      0    2233    3507 
   Size:       2233    1274
 
BsaWI w'CCGG_w

Cuts at:      0    1370    1607    2446    3507 
   Size:       1370     237     839    1061

  Fragments arranged by size: 

                1370    1061     839     237
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1663    1693    2212    2242    3507 
   Size:       1663      30     519      30    1265

  Fragments arranged by size: 

                1663    1265     519      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      34      43     273     276     389     758    1926    3064 
   Size:         34       9     230       3     113     369    1168    1138

Cuts at:   3064    3198    3507 
   Size:        134     309

  Fragments arranged by size: 

                1168    1138     369     309     230     134     113      34
                   9       3
 
BseYI C'CCAG_C

Cuts at:      0     193    2125    3507 
   Size:        193    1932    1382

  Fragments arranged by size: 

                1932    1382     193
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1669    3507 
   Size:       1669    1838
 
BsiEI CG_ry'CG

Cuts at:      0     307    3507 
   Size:        307    3200
 
BsiHKAI G_wGCw'C

Cuts at:      0    2163    3507 
   Size:       2163    1344
 
BsmI GAATG_Cn'

Cuts at:      0     838    2247    3507 
   Size:        838    1409    1260

  Fragments arranged by size: 

                1409    1260     838
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1701    3507 
   Size:       1701    1806
 
Bsp1286I G_dGCh'C

Cuts at:      0     527     741     787    1642    2163    2338    2375    2419 
   Size:        527     214      46     855     521     175      37      44

Cuts at:   2419    2790    3031    3507 
   Size:        371     241     476

  Fragments arranged by size: 

                 855     527     521     476     371     241     214     175
                  46      44      37
 
BspEI T'CCGG_A

Cuts at:      0    2446    3507 
   Size:       2446    1061
 
BspHI T'CATG_A

Cuts at:      0    2495    2870    3507 
   Size:       2495     375     637

  Fragments arranged by size: 

                2495     637     375
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     375    1289    2276    3012    3507 
   Size:        375     914     987     736     495

  Fragments arranged by size: 

                 987     914     736     495     375
 
BsrBI CCG'CTC

Cuts at:      0    1722    1785    3507 
   Size:       1722      63    1722

  Fragments arranged by size: 

                1722    1722      63
 
BsrDI GCAATG_nn'

Cuts at:      0    2093    3507 
   Size:       2093    1414
 
BsrFI r'CCGG_y

Cuts at:      0    1874    3507 
   Size:       1874    1633
 
BsrGI T'GTAC_A

Cuts at:      0     731    3507 
   Size:        731    2776
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     600    3507 
   Size:        600    2907
 
BstEII G'GTnAC_C

Cuts at:      0    2650    3507 
   Size:       2650     857
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     996    1551    3507 
   Size:        996     555    1956

  Fragments arranged by size: 

                1956     996     555
 
BstYI r'GATC_y

Cuts at:      0      87     374    1601    1931    2381    3507 
   Size:         87     287    1227     330     450    1126

  Fragments arranged by size: 

                1227    1126     450     330     287      87
 
BtgI C'CryG_G

Cuts at:      0     518    1643    2661    3507 
   Size:        518    1125    1018     846

  Fragments arranged by size: 

                1125    1018     846     518
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     216     822     849    1434    3084    3123    3507 
   Size:        216     606      27     585    1650      39     384

  Fragments arranged by size: 

                1650     606     585     384     216      39      27
 
BtsI GCAGTG_nn'

Cuts at:      0     300    1414    2751    3507 
   Size:        300    1114    1337     756

  Fragments arranged by size: 

                1337    1114     756     300
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     864     899    3507 
   Size:        864      35    2608

  Fragments arranged by size: 

                2608     864      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     138    2330    3507 
   Size:        138    2192    1177

  Fragments arranged by size: 

                2192    1177     138
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     544    1103    1972    3507 
   Size:        544     559     869    1535

  Fragments arranged by size: 

                1535     869     559     544
 
EaeI y'GGCC_r

Cuts at:      0       2     304    1196    1286    1628    1838    1867    1904 
   Size:          2     302     892      90     342     210      29      37

Cuts at:   1904    3414    3507 
   Size:       1510      93

  Fragments arranged by size: 

                1510     892     342     302     210      93      90      37
                  29       2
 
EagI C'GGCC_G

Cuts at:      0     304    3507 
   Size:        304    3203
 
EarI CTCTTCn'nnn_

Cuts at:      0    2620    2686    3248    3507 
   Size:       2620      66     562     259

  Fragments arranged by size: 

                2620     562     259      66
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      80     367    1564    2307    3507 
   Size:         80     287    1197     743    1200

  Fragments arranged by size: 

                1200    1197     743     287      80
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     445    1600    1675    1726    1909    2498    2503    2604 
   Size:        445    1155      75      51     183     589       5     101

Cuts at:   2604    2623    2725    2842    3260    3507 
   Size:         19     102     117     418     247

  Fragments arranged by size: 

                1155     589     445     418     247     183     117     102
                 101      75      51      19       5
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1840    3507 
   Size:       1840    1667
 
EcoO109I rG'GnC_Cy

Cuts at:      0     240     272     678     849     888     930    1884    2415 
   Size:        240      32     406     171      39      42     954     531

Cuts at:   2415    2416    3507 
   Size:          1    1091

  Fragments arranged by size: 

                1091     954     531     406     240     171      42      39
                  32       1
 
HaeII r_GCGC'y

Cuts at:      0     449    2145    3014    3507 
   Size:        449    1696     869     493

  Fragments arranged by size: 

                1696     869     493     449
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     880     912    1193    1225    2413    2445    2870    2902 
   Size:        880      32     281      32    1188      32     425      32

Cuts at:   2902    2971    3003    3460    3492    3507 
   Size:         69      32     457      32      15

  Fragments arranged by size: 

                1188     880     457     425     281      69      32      32
                  32      32      32      32      15
 
HincII GTy'rAC

Cuts at:      0    2043    2076    3507 
   Size:       2043      33    1431

  Fragments arranged by size: 

                2043    1431      33
 
HindIII A'AGCT_T

Cuts at:      0     765    3074    3507 
   Size:        765    2309     433

  Fragments arranged by size: 

                2309     765     433
 
HpaI GTT'AAC

Cuts at:      0    2043    3507 
   Size:       2043    1464
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     605    1013    1289    2022    2564    3507 
   Size:        605     408     276     733     542     943

  Fragments arranged by size: 

                 943     733     605     542     408     276
 
MscI TGG'CCA

Cuts at:      0       4    1288    1630    1840    3507 
   Size:          4    1284     342     210    1667

  Fragments arranged by size: 

                1667    1284     342     210       4
 
MslI CAynn'nnrTG

Cuts at:      0    1354    1419    1472    1645    2861    3507 
   Size:       1354      65      53     173    1216     646

  Fragments arranged by size: 

                1354    1216     646     173      65      53
 
MspA1I CmG'CkG

Cuts at:      0    1653    3507 
   Size:       1653    1854
 
NcoI C'CATG_G

Cuts at:      0     518    3507 
   Size:        518    2989
 
NsiI A_TGCA'T

Cuts at:      0     838    3507 
   Size:        838    2669
 
NspI r_CATG'y

Cuts at:      0     603     732    3507 
   Size:        603     129    2775

  Fragments arranged by size: 

                2775     603     129
 
PasI CC'CwG_GG

Cuts at:      0      23     780    2462    3108    3507 
   Size:         23     757    1682     646     399

  Fragments arranged by size: 

                1682     757     646     399      23
 
PciI A'CATG_T

Cuts at:      0     728    3507 
   Size:        728    2779
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1143    1836    3507 
   Size:       1143     693    1671

  Fragments arranged by size: 

                1671    1143     693
 
PfoI T'CCnGG_A

Cuts at:      0    1217    3507 
   Size:       1217    2290
 
PmlI CAC'GTG

Cuts at:      0    1805    2228    3507 
   Size:       1805     423    1279

  Fragments arranged by size: 

                1805    1279     423
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3373    3405    3507 
   Size:       3373      32     102

  Fragments arranged by size: 

                3373     102      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     240     272     678     849     888    1884    3507 
   Size:        240      32     406     171      39     996    1623

  Fragments arranged by size: 

                1623     996     406     240     171      39      32
 
PshAI GACnn'nnGTC

Cuts at:      0    2362    3507 
   Size:       2362    1145
 
PsiI TTA'TAA

Cuts at:      0     698    3507 
   Size:        698    2809
 
PspOMI G'GGCC_C

Cuts at:      0    2415    3507 
   Size:       2415    1092
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2518    2550    3507 
   Size:       2518      32     957

  Fragments arranged by size: 

                2518     957      32
 
PstI C_TGCA'G

Cuts at:      0     386     596    3302    3507 
   Size:        386     210    2706     205

  Fragments arranged by size: 

                2706     386     210     205
 
PvuII CAG'CTG

Cuts at:      0    1653    3507 
   Size:       1653    1854
 
SanDI GG'GwC_CC

Cuts at:      0     272     849    3507 
   Size:        272     577    2658

  Fragments arranged by size: 

                2658     577     272
 
SbfI CC_TGCA'GG

Cuts at:      0     386    3302    3507 
   Size:        386    2916     205

  Fragments arranged by size: 

                2916     386     205
 
SexAI A'CCwGG_T

Cuts at:      0    1622    3507 
   Size:       1622    1885
 
SfcI C'TryA_G

Cuts at:      0     382     592     612    2688    3273    3298    3507 
   Size:        382     210      20    2076     585      25     209

  Fragments arranged by size: 

                2076     585     382     210     209      25      20
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1875    3507 
   Size:       1875    1632
 
SmaI CCC'GGG

Cuts at:      0     247    1882    1938    3507 
   Size:        247    1635      56    1569

  Fragments arranged by size: 

                1635    1569     247      56
 
SmlI C'TyrA_G

Cuts at:      0     673     711    2303    3507 
   Size:        673      38    1592    1204

  Fragments arranged by size: 

                1592    1204     673      38
 
StuI AGG'CCT

Cuts at:      0    1846    2311    3507 
   Size:       1846     465    1196

  Fragments arranged by size: 

                1846    1196     465
 
StyI C'CwwG_G

Cuts at:      0     236     410     518    1841    2753    2791    3231    3248 
   Size:        236     174     108    1323     912      38     440      17

Cuts at:   3248    3507 
   Size:        259

  Fragments arranged by size: 

                1323     912     440     259     236     174     108      38
                  17
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     547     622     655    1240    1912    2604    3121    3507 
   Size:        547      75      33     585     672     692     517     386

  Fragments arranged by size: 

                 692     672     585     547     517     386      75      33
            
 
TatI w'GTAC_w

Cuts at:      0     731    3419    3507 
   Size:        731    2688      88

  Fragments arranged by size: 

                2688     731      88
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     316     348    1663    1695    2212    2244    3507 
   Size:        316      32    1315      32     517      32    1263

  Fragments arranged by size: 

                1315    1263     517     316      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     427    1969    2545    3507 
   Size:        427    1542     576     962

  Fragments arranged by size: 

                1542     962     576     427
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      20     866    1548    1650    1773    1999    3021    3144 
   Size:         20     846     682     102     123     226    1022     123

Cuts at:   3144    3507 
   Size:        363

  Fragments arranged by size: 

                1022     846     682     363     226     123     123     102
                  20
 
XhoI C'TCGA_G

Cuts at:      0    2303    3507 
   Size:       2303    1204
 
XmaI C'CCGG_G

Cuts at:      0     245    1880    1936    3507 
   Size:        245    1635      56    1571

  Fragments arranged by size: 

                1635    1571     245      56
 
XmnI GAAnn'nnTTC

Cuts at:      0     664    3507 
   Size:        664    2843
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI    AflII   AflIII     AhdI     AleI
     AlfI     ApaI     ApoI     AvaI    AvrII    BamHI     BanI    BanII
     BbsI    BbvCI    BciVI    BfrBI     BglI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI    CspCI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I    HaeII    Hin4I   HincII  HindIII     HpaI     MmeI     MscI
     MslI   MspA1I     NcoI     NsiI     NspI     PasI     PciI    PflMI
     PfoI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PsrI
     PstI    PvuII    SanDI     SbfI    SexAI     SfcI     SfiI     SmaI
     SmlI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AgeI     AloI    AlwNI    ApaLI     AscI
     AseI    AsiSI     BaeI     BbeI     BcgI     BclI    BglII     BlpI
     BmtI    BsaBI    BsiWI   BssHII    BssSI    BstBI  BstZ17I   Bsu36I
     ClaI     DraI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI     KasI     KpnI     MfeI     MluI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PmeI    PspXI     PvuI
    RsrII     SacI    SacII     SalI     SapI     ScaI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     XbaI
     ZraI
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