(Circular) (Six-base) MAPSORT of: pF1KA0750.seq Check: 7415 from: 1 to: 6485 pF1KA0750 6485 bp With 182 enzymes: SgfI * June 20, 2008 10:20 .. AatII G_ACGT'C Cuts at: 761 761 Size: 6485 AccI GT'mk_AC Cuts at: 3523 3523 Size: 6485 Acc65I G'GTAC_C Cuts at: 187 1517 3501 187 Size: 1330 1984 3171 Fragments arranged by size: 3171 1984 1330 AclI AA'CG_TT Cuts at: 6325 6325 Size: 6485 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 698 2891 3017 4310 4742 5489 5757 698 Size: 2193 126 1293 432 747 268 1426 Fragments arranged by size: 2193 1426 1293 747 432 268 126 AflIII A'CryG_T Cuts at: 1496 3107 3109 3129 3837 4941 5918 1496 Size: 1611 2 20 708 1104 977 2063 Fragments arranged by size: 2063 1611 1104 977 708 20 2 AgeI A'CCGG_T Cuts at: 4873 4873 Size: 6485 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 195 2513 195 Size: 2318 4167 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2931 2963 2931 Size: 32 6453 AlwNI CAG_nnn'CTG Cuts at: 1029 2274 3200 3790 5357 1029 Size: 1245 926 590 1567 2157 Fragments arranged by size: 2157 1567 1245 926 590 ApaLI G'TGCA_C Cuts at: 357 5255 357 Size: 4898 1587 ApoI r'AATT_y Cuts at: 715 814 925 2571 2692 3253 3489 5838 6094 Size: 99 111 1646 121 561 236 2349 256 Cuts at: 6094 715 Size: 1106 Fragments arranged by size: 2349 1646 1106 561 256 236 121 111 99 AseI AT'TA_AT Cuts at: 20 20 Size: 6485 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6485 AvaI C'yCGr_G Cuts at: 1124 1600 2598 3505 1124 Size: 476 998 907 4104 Fragments arranged by size: 4104 998 907 476 BamHI G'GATC_C Cuts at: 1874 3510 1874 Size: 1636 4849 BanI G'GyrC_C Cuts at: 187 769 1399 1517 1682 1975 3418 3501 3915 Size: 582 630 118 165 293 1443 83 414 Cuts at: 3915 4163 4198 187 Size: 248 35 2474 Fragments arranged by size: 2474 1443 630 582 414 293 248 165 118 83 35 BanII G_rGCy'C Cuts at: 1615 1793 1967 2203 2486 2673 3499 4529 1615 Size: 178 174 236 283 187 826 1030 3571 Fragments arranged by size: 3571 1030 826 283 236 187 178 174 BbeI G_GCGC'C Cuts at: 3919 4167 3919 Size: 248 6237 BbvCI CC'TCA_GC Cuts at: 2032 2951 2032 Size: 919 5566 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2253 2287 2253 Size: 34 6451 BciVI GTATCCnnnnn_n' Cuts at: 4383 5144 6485 4383 Size: 761 1341 4383 Fragments arranged by size: 4383 1341 761 BclI T'GATC_A Cuts at: 3024 4005 3024 Size: 981 5504 BglI GCCn_nnn'nGGC Cuts at: 1320 1320 Size: 6485 BglII A'GATC_T Cuts at: 960 1624 4000 6037 960 Size: 664 2376 2037 1408 Fragments arranged by size: 2376 2037 1408 664 BlpI GC'TnA_GC Cuts at: 1930 3151 3597 1930 Size: 1221 446 4818 Fragments arranged by size: 4818 1221 446 Bme1580I G_kGCm'C Cuts at: 361 1344 1402 1978 2471 4110 4203 5259 361 Size: 983 58 576 493 1639 93 1056 1587 Fragments arranged by size: 1639 1587 1056 983 576 493 93 58 BmrI ACTGGGnnnn_n' Cuts at: 1618 3433 3697 3778 4113 6257 1618 Size: 1815 264 81 335 2144 1846 Fragments arranged by size: 2144 1846 1815 335 264 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3398 4852 3398 Size: 1454 5031 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 178 1432 1606 1623 1865 2644 3038 178 Size: 1254 174 17 242 779 394 3625 Fragments arranged by size: 3625 1254 779 394 242 174 17 Bpu10I CC'TnA_GC Cuts at: 1787 2032 2515 2951 1787 Size: 245 483 436 5321 Fragments arranged by size: 5321 483 436 245 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 250 985 1330 2470 3661 4540 5032 5330 250 Size: 735 345 1140 1191 879 492 298 1405 Fragments arranged by size: 1405 1191 1140 879 735 492 345 298 BsaI GGTCTCn'nnnn_ Cuts at: 6189 6189 Size: 6485 BsaAI yAC'GTr Cuts at: 179 2472 3110 3138 3838 179 Size: 2293 638 28 700 2826 Fragments arranged by size: 2826 2293 700 638 28 BsaBI GATnn'nnATC Cuts at: 6 838 3999 6036 6 Size: 832 3161 2037 455 Fragments arranged by size: 3161 2037 832 455 BsaHI Gr'CG_yC Cuts at: 758 3916 4164 4866 6362 758 Size: 3158 248 702 1496 881 Fragments arranged by size: 3158 1496 881 702 248 BsaWI w'CCGG_w Cuts at: 2116 3674 4195 4873 5147 5294 5705 2116 Size: 1558 521 678 274 147 411 2896 Fragments arranged by size: 2896 1558 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 425 454 455 484 736 766 2719 2749 2787 Size: 29 1 29 252 30 1953 30 38 Cuts at: 2787 2817 425 Size: 30 4093 Fragments arranged by size: 4093 1953 252 38 30 30 30 29 29 1 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1635 2302 2419 2420 2801 3352 3355 1635 Size: 667 117 1 381 551 3 4765 Fragments arranged by size: 4765 667 551 381 117 3 1 BseYI C'CCAG_C Cuts at: 624 1663 2416 2556 2926 3910 4668 5245 5741 Size: 1039 753 140 370 984 758 577 496 Cuts at: 5741 624 Size: 1368 Fragments arranged by size: 1368 1039 984 758 753 577 496 370 140 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 975 975 Size: 6485 BsiEI CG_ry'CG Cuts at: 104 4027 4073 5281 104 Size: 3923 46 1208 1308 Fragments arranged by size: 3923 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 361 1592 1793 1967 2296 2362 2477 2486 3499 Size: 1231 201 174 329 66 115 9 1013 Cuts at: 3499 4277 4467 5259 361 Size: 778 190 792 1587 Fragments arranged by size: 1587 1231 1013 792 778 329 201 190 174 115 66 9 BsmBI CGTCTCn'nnnn_ Cuts at: 2136 2834 3303 2136 Size: 698 469 5318 Fragments arranged by size: 5318 698 469 Bsp1286I G_dGCh'C Cuts at: 361 1344 1402 1592 1615 1793 1967 1978 2203 Size: 983 58 190 23 178 174 11 225 Cuts at: 2203 2296 2362 2471 2477 2486 2673 3499 4110 Size: 93 66 109 6 9 187 826 611 Cuts at: 4110 4203 4277 4467 4529 5259 361 Size: 93 74 190 62 730 1587 Fragments arranged by size: 1587 983 826 730 611 225 190 190 187 178 174 109 93 93 74 66 62 58 23 11 9 6 BspEI T'CCGG_A Cuts at: 5705 5705 Size: 6485 BspHI T'CATG_A Cuts at: 2312 5661 2312 Size: 3349 3136 BspMI ACCTGCnnnn'nnnn_ Cuts at: 138 1118 1231 3535 3749 4051 4432 138 Size: 980 113 2304 214 302 381 2191 Fragments arranged by size: 2304 2191 980 381 302 214 113 BsrBI CCG'CTC Cuts at: 50 1263 4777 4831 6315 6480 50 Size: 1213 3514 54 1484 165 55 Fragments arranged by size: 3514 1484 1213 165 55 54 BsrDI GCAATG_nn' Cuts at: 4397 4397 Size: 6485 BsrFI r'CCGG_y Cuts at: 1442 2349 4483 4664 4873 1442 Size: 907 2134 181 209 3054 Fragments arranged by size: 3054 2134 907 209 181 BsrGI T'GTAC_A Cuts at: 3014 3014 Size: 6485 BssSI C'ACGA_G Cuts at: 1282 4756 5114 1282 Size: 3474 358 2653 Fragments arranged by size: 3474 2653 358 BstAPI GCAn_nnn'nTGC Cuts at: 1029 1141 1278 2510 3862 1029 Size: 112 137 1232 1352 3652 Fragments arranged by size: 3652 1352 1232 137 112 BstBI TT'CG_AA Cuts at: 4846 4846 Size: 6485 BstEII G'GTnAC_C Cuts at: 276 276 Size: 6485 BstYI r'GATC_y Cuts at: 1 960 1624 1874 3510 3977 4000 4335 4581 Size: 959 664 250 1636 467 23 335 246 Cuts at: 4581 5593 5679 5691 6037 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1636 1012 959 664 467 449 346 335 250 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 3035 3035 Size: 6485 BtgI C'CryG_G Cuts at: 107 819 1325 1344 1889 1991 2098 2258 107 Size: 712 506 19 545 102 107 160 4334 Fragments arranged by size: 4334 712 545 506 160 107 102 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3257 4435 4616 4779 3257 Size: 1178 181 163 4963 Fragments arranged by size: 4963 1178 181 163 BtsI GCAGTG_nn' Cuts at: 407 1752 2511 3410 3728 407 Size: 1345 759 899 318 3164 Fragments arranged by size: 3164 1345 899 759 318 DraI TTT'AAA Cuts at: 3484 3484 Size: 6485 DraIII CAC_nnn'GTG Cuts at: 371 1540 371 Size: 1169 5316 DrdI GACnn_nn'nnGTC Cuts at: 1042 4191 5049 1042 Size: 3149 858 2478 Fragments arranged by size: 3149 2478 858 EaeI y'GGCC_r Cuts at: 777 1205 1856 2039 2276 2681 3221 3407 4070 Size: 428 651 183 237 405 540 186 663 Cuts at: 4070 4244 4635 4662 6007 777 Size: 174 391 27 1345 1255 Fragments arranged by size: 1345 1255 663 651 540 428 405 391 237 186 183 174 27 EagI C'GGCC_G Cuts at: 4070 4070 Size: 6485 EarI CTCTTCn'nnn_ Cuts at: 451 884 1378 1972 2083 2367 2659 3301 4508 Size: 433 494 594 111 284 292 642 1207 Cuts at: 4508 4718 451 Size: 210 2218 Fragments arranged by size: 2218 1207 642 594 494 433 292 284 210 111 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 4693 5003 5149 6295 4693 Size: 310 146 1146 4883 Fragments arranged by size: 4883 1146 310 146 EcoICRI GAG'CTC Cuts at: 1791 1965 2484 3497 1791 Size: 174 519 1013 4779 Fragments arranged by size: 4779 1013 519 174 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 178 698 1432 1606 1623 1865 2644 2891 3017 Size: 520 734 174 17 242 779 247 126 Cuts at: 3017 3038 4310 4742 5489 5757 178 Size: 21 1272 432 747 268 906 Fragments arranged by size: 1272 906 779 747 734 520 432 268 247 242 174 126 21 17 EcoNI CCTnn'n_nnAGG Cuts at: 607 607 Size: 6485 EcoO109I rG'GnC_Cy Cuts at: 391 522 936 1547 3624 391 Size: 131 414 611 2077 3252 Fragments arranged by size: 3252 2077 611 414 131 EcoRI G'AATT_C Cuts at: 814 3489 814 Size: 2675 3810 EcoRV GAT'ATC Cuts at: 1656 1656 Size: 6485 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3288 4504 3288 Size: 1216 5269 FspI TGC'GCA Cuts at: 406 3402 4266 406 Size: 2996 864 2625 Fragments arranged by size: 2996 2625 864 HaeII r_GCGC'y Cuts at: 3158 3919 4167 5189 6168 3158 Size: 761 248 1022 979 3475 Fragments arranged by size: 3475 1022 979 761 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 454 486 620 652 1891 1923 3049 3081 4327 Size: 32 134 32 1239 32 1126 32 1246 Cuts at: 4327 4357 4359 4389 454 Size: 30 2 30 2550 Fragments arranged by size: 2550 1246 1239 1126 134 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1632 1716 2868 3524 1632 Size: 84 1152 656 4593 Fragments arranged by size: 4593 1152 656 84 KasI G'GCGC_C Cuts at: 3915 4163 3915 Size: 248 6237 KpnI G_GTAC'C Cuts at: 191 1521 3505 191 Size: 1330 1984 3171 Fragments arranged by size: 3171 1984 1330 MluI A'CGCG_T Cuts at: 5918 5918 Size: 6485 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 722 1481 5156 5340 722 Size: 759 3675 184 1867 Fragments arranged by size: 3675 1867 759 184 MscI TGG'CCA Cuts at: 1207 2041 2278 2683 4246 1207 Size: 834 237 405 1563 3446 Fragments arranged by size: 3446 1563 834 405 237 MslI CAynn'nnrTG Cuts at: 177 644 1072 1939 4474 4601 177 Size: 467 428 867 2535 127 2061 Fragments arranged by size: 2535 2061 867 467 428 127 MspA1I CmG'CkG Cuts at: 874 1158 1278 2271 2556 2842 2955 2978 2995 Size: 284 120 993 285 286 113 23 17 Cuts at: 2995 3191 3596 3910 4270 5283 5528 874 Size: 196 405 314 360 1013 245 1831 Fragments arranged by size: 1831 1013 993 405 360 314 286 285 284 245 196 120 113 23 17 NaeI GCC'GGC Cuts at: 4666 4666 Size: 6485 NarI GG'CG_CC Cuts at: 3916 4164 3916 Size: 248 6237 NcoI C'CATG_G Cuts at: 107 1325 2258 107 Size: 1218 933 4334 Fragments arranged by size: 4334 1218 933 NgoMIV G'CCGG_C Cuts at: 4664 4664 Size: 6485 NspI r_CATG'y Cuts at: 1230 1714 3538 4038 4945 1230 Size: 484 1824 500 907 2770 Fragments arranged by size: 2770 1824 907 500 484 PasI CC'CwG_GG Cuts at: 1507 1507 Size: 6485 PciI A'CATG_T Cuts at: 4941 4941 Size: 6485 PflMI CCAn_nnn'nTGG Cuts at: 1739 3475 1739 Size: 1736 4749 PfoI T'CCnGG_A Cuts at: 472 1639 472 Size: 1167 5318 PmeI GTTT'AAAC Cuts at: 3484 3484 Size: 6485 PmlI CAC'GTG Cuts at: 179 2472 3110 179 Size: 2293 638 3554 Fragments arranged by size: 3554 2293 638 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5624 5656 5624 Size: 32 6453 PpuMI rG'GwC_Cy Cuts at: 391 936 391 Size: 545 5940 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 961 993 961 Size: 32 6453 PstI C_TGCA'G Cuts at: 1137 2639 2994 3196 3532 4217 1137 Size: 1502 355 202 336 685 3405 Fragments arranged by size: 3405 1502 685 355 336 202 PvuI CG_AT'CG Cuts at: 104 104 Size: 6485 PvuII CAG'CTG Cuts at: 1158 1278 2271 2556 2842 2955 2995 3910 4270 Size: 120 993 285 286 113 40 915 360 Cuts at: 4270 1158 Size: 3373 Fragments arranged by size: 3373 993 915 360 286 285 120 113 40 RsrII CG'GwC_CG Cuts at: 4680 4680 Size: 6485 SacI G_AGCT'C Cuts at: 1793 1967 2486 3499 1793 Size: 174 519 1013 4779 Fragments arranged by size: 4779 1013 519 174 SalI G'TCGA_C Cuts at: 3522 3522 Size: 6485 SapI GCTCTTCn'nnn_ Cuts at: 1378 1972 2367 4508 4718 1378 Size: 594 395 2141 210 3145 Fragments arranged by size: 3145 2141 594 395 210 SbfI CC_TGCA'GG Cuts at: 3532 3532 Size: 6485 ScaI AGT'ACT Cuts at: 6002 6002 Size: 6485 SexAI A'CCwGG_T Cuts at: 1678 1678 Size: 6485 SfcI C'TryA_G Cuts at: 33 592 725 1133 2551 2635 2990 3192 3528 Size: 559 133 408 1418 84 355 202 336 Cuts at: 3528 4213 5206 5397 33 Size: 685 993 191 1121 Fragments arranged by size: 1418 1121 993 685 559 408 355 336 202 191 133 84 SfoI GGC'GCC Cuts at: 3917 4165 3917 Size: 248 6237 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6485 SmaI CCC'GGG Cuts at: 1126 3507 1126 Size: 2381 4104 SmlI C'TyrA_G Cuts at: 265 964 1309 1600 2485 3640 4555 5047 5309 Size: 699 345 291 885 1155 915 492 262 Cuts at: 5309 265 Size: 1441 Fragments arranged by size: 1441 1155 915 885 699 492 345 291 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 6485 SphI G_CATG'C Cuts at: 3538 4038 3538 Size: 500 5985 StuI AGG'CCT Cuts at: 199 2786 199 Size: 2587 3898 StyI C'CwwG_G Cuts at: 107 272 881 1325 2083 2258 2524 3619 3973 Size: 165 609 444 758 175 266 1095 354 Cuts at: 3973 107 Size: 2619 Fragments arranged by size: 2619 1095 758 609 444 354 266 175 165 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1009 1285 2430 2548 3482 4068 4201 4660 4869 Size: 276 1145 118 934 586 133 459 209 Cuts at: 4869 1009 Size: 2625 Fragments arranged by size: 2625 1145 934 586 459 276 209 133 118 TatI w'GTAC_w Cuts at: 1493 3014 6000 1493 Size: 1521 2986 1978 Fragments arranged by size: 2986 1978 1521 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 425 457 540 572 2719 2751 6002 6034 425 Size: 32 83 32 2147 32 3251 32 876 Fragments arranged by size: 3251 2147 876 83 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 4282 4282 Size: 6485 XbaI T'CTAG_A Cuts at: 666 3516 666 Size: 2850 3635 XcmI CCAnnnn_n'nnnnTGG Cuts at: 444 2193 3216 3420 3980 444 Size: 1749 1023 204 560 2949 Fragments arranged by size: 2949 1749 1023 560 204 XhoI C'TCGA_G Cuts at: 1600 1600 Size: 6485 XmaI C'CCGG_G Cuts at: 1124 3505 1124 Size: 2381 4104 XmnI GAAnn'nnTTC Cuts at: 2152 2654 2152 Size: 502 5983 ZraI GAC'GTC Cuts at: 759 759 Size: 6485 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AflII AhdI AleI ApaI AscI AvrII BaeI BbsI BfrBI BmgBI BmtI BsiWI BsmI BssHII BstXI BstZ17I ClaI CspCI FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PshAI PsiI PspOMI PspXI SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI