Restriction map for pF1KA0714
##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        Asp718I     AspEI       AviII       BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BglI        BsaHI       BseAI       BsiEI       BsiWI       
BspDI       BspEI       BsrBI       BsrFI       BssHII      
BssSI       BstBI       BstEII      BstPI       Cfr10I      
ClaI        CpoI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     FseI        FspI        
HaeII       Hin1I       HpaI        KasI        KpnI        
KspI        MluI        MroI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PvuI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SpeI        
SphI        Sse8387I    SwaI        Van91I      XcmI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII         358  CGCG                 CG^CG
AclI         5120  AACGTT               AA^CGTT
AflII        2146  CTTAAG               C^TTAAG
AocI           36  CCTGAGG              CC^T[ACGT]AGG
AspI         1774  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII         132  CCTAGG               C^CTAGG
BalI          316  TGGCCA               TGG^CCA
BcnI          666  CCGGG                CC^[GC]GG
BfrI         2146  CTTAAG               C^TTAAG
BlnI          132  CCTAGG               C^CTAGG
BsaBI        2806  GATTCTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         3263  GGTCTC               GGTCTC(1/5)
BsgI         1975  GTGCAG               GTGCAG(16/14)
BspLU11I     2340  ACATGT               A^CATGT
BspMI        3441  ACCTGC               ACCTGC(4/8)
BsrDI        4021  GCAATG               GCAATG(2/0)
Bst1107I     3437  GTATAC               GTA^TAC
BstAPI        496  GCAAAAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         358  CGCG                 CG^CG
BstZ17I      3437  GTATAC               GTA^TAC
Bsu36I         36  CCTGAGG              CC^T[ACGT]AGG
CfoI          852  GCGC                 GCG^C
DraI         1888  TTTAAA               TTT^AAA
DsaI         4730  CCATGG               C^C[GA][CT]GG
Eco81I         36  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1551  CCTATTACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        2136  GAATTC               G^AATTC
EcoT22I       503  ATGCAT               ATGCA^T
HgaI          359  GCGTC                GACGC(5/10)
HhaI          852  GCGC                 GCG^C
HinP1I        852  GCGC                 G^CGC
MamI         2806  GATTCTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          929  CAATTG               C^AATTG
MluNI         316  TGGCCA               TGG^CCA
MscI          316  TGGCCA               TGG^CCA
MunI          929  CAATTG               C^AATTG
MvnI          358  CGCG                 CG^CG
NciI          666  CCGGG                CC^[GC]GG
NcoI         4730  CCATGG               C^CATGG
NsiI          503  ATGCAT               ATGCA^T
PflFI        1774  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I     5120  AACGTT               AA^CGTT
SnaBI        3177  TACGTA               TAC^GTA
Tth111I      1774  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         2793  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccI         3437  GTATAC               GT^[AC][GT]AC
             5113  GTAGAC              
AflIII       2340  ACATGT               A^C[GA][CT]GT
             3192  ACGTGT              
AseI         1804  ATTAAT               AT^TAAT
             4446  ATTAAT              
AsnI         1804  ATTAAT               AT^TAAT
             4446  ATTAAT              
AvaI         4883  CTCGAG               C^[CT]CG[GA]G
             4895  CTCGAG              
BbsI         1771  GAAGAC               GAAGAC(2/6)
             4405  GAAGAC              
BciVI        3879  GGATAC               GTATCC(6/5)
             4426  GGATAC              
BglII        1487  AGATCT               A^GATCT
             3468  AGATCT              
BpuAI        1771  GAAGAC               GAAGAC(2/6)
             4405  GAAGAC              
BsaAI        3177  TACGTA               [CT]AC^GT[GA]
             3191  TACGTG              
BsaWI        2066  TCCGGT               [AT]^CCGG[AT]
             3674  TCCGGT              
BsmFI        3731  GGGAC                GGGAC(10/14)
             4597  GTCCC               
BsoBI        4883  CTCGAG               C^[CT]CG[GA]G
             4895  CTCGAG              
BspHI         348  TCATGA               T^CATGA
             2400  TCATGA              
BstYI        1487  AGATCT               [GA]^GATC[CT]
             3468  AGATCT              
DraII        4595  AGGTCCC              [GA]G^G[ACGT]CC[CT]
             4607  AGGACCC             
DrdI          352  GACCGTCGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              925  GACCCAATTGTC        
EaeI           54  TGGCCG               [CT]^GGCC[GA]
              316  TGGCCA              
EcoO109I     4595  AGGTCCC              [GA]G^G[ACGT]CC[CT]
             4607  AGGACCC             
EcoRV        2215  GATATC               GAT^ATC
             2737  GATATC              
FauI          624  CCCGC                CCCGC(4/6)
                5  GCGGG               
HincII       1179  GTCAAC               GT[CT]^[GA]AC
             2236  GTCAAC              
HindII       1179  GTCAAC               GT[CT]^[GA]AC
             2236  GTCAAC              
MflI         1487  AGATCT               [GA]^GATC[CT]
             3468  AGATCT              
NdeI         4207  CATATG               CA^TATG
             4705  CATATG              
PaeR7I       4883  CTCGAG               C^TCGAG
             4895  CTCGAG              
PpuMI        4595  AGGTCCC              [GA]G^G[AT]CC[CT]
             4607  AGGACCC             
PshBI        1804  ATTAAT               AT^TAAT
             4446  ATTAAT              
RcaI          348  TCATGA               T^CATGA
             2400  TCATGA              
SacI         1578  GAGCTC               GAGCT^C
             4633  GAGCTC              
TaqI         4884  TCGA                 T^CGA
             4896  TCGA                
TthHB8I      4884  TCGA                 T^CGA
             4896  TCGA                
XhoI         4883  CTCGAG               C^TCGAG
             4895  CTCGAG              
XhoII        1487  AGATCT               [GA]^GATC[CT]
             3468  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AspHI       AvaII       
BanII       BbvI        BclI        BfaI        BlpI        
BmyI        BpmI        Bpu1102I    BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmI        Bsp1286I    Bsp1407I    BsrGI       BsrI        
BssKI       BstF5I      BstNI       BstXI       Cac8I       
CelII       Cfr13I      DdeI        DpnII       DraIII      
EarI        EcoRII      EcoT14I     FbaI        Fnu4HI      
FokI        HaeIII      HapII       HindIII     HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PstI        PvuII       RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspBI       SspI        StuI        StyI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XmnI        
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