Restriction map for pF1KA0714
 (Linear) (Six-base) MAPSORT of: KIAA0714.seq  Check: 600  from: 1  to: 5298



KIAA0714.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:21  ..

AccI GT'mk_AC

Cuts at:      0    3438    5114    5298 
   Size:       3438    1676     184

  Fragments arranged by size: 

                3438    1676     184
 
AclI AA'CG_TT

Cuts at:      0    5121    5298 
   Size:       5121     177
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     560    1190    1549    1655    1718    2270    2549 
   Size:        182     378     630     359     106      63     552     279

Cuts at:   2549    2728    2984    3380    4158    5298 
   Size:        179     256     396     778    1140

  Fragments arranged by size: 

                1140     778     630     552     396     378     359     279
                 256     182     179     106      63
 
AflII C'TTAA_G

Cuts at:      0    2146    5298 
   Size:       2146    3152
 
AflIII A'CryG_T

Cuts at:      0    2340    3192    5298 
   Size:       2340     852    2106

  Fragments arranged by size: 

                2340    2106     852
 
AleI CACnn'nnGTG

Cuts at:      0    3102    5298 
   Size:       3102    2196
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3833    5298 
   Size:       3833    1465
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    5000    5032    5298 
   Size:       5000      32     266

  Fragments arranged by size: 

                5000     266      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      97     292    1519    1995    2437    5298 
   Size:         97     195    1227     476     442    2861

  Fragments arranged by size: 

                2861    1227     476     442     195      97
 
ApoI r'AATT_y

Cuts at:      0     256     323     335     658     748    1149    2136    2637 
   Size:        256      67      12     323      90     401     987     501

Cuts at:   2637    3421    3925    5218    5298 
   Size:        784     504    1293      80

  Fragments arranged by size: 

                1293     987     784     504     501     401     323     256
                  90      80      67      12
 
AseI AT'TA_AT

Cuts at:      0    1805    4447    5298 
   Size:       1805    2642     851

  Fragments arranged by size: 

                2642    1805     851
 
AvaI C'yCGr_G

Cuts at:      0    4883    4895    5298 
   Size:       4883      12     403

  Fragments arranged by size: 

                4883     403      12
 
AvrII C'CTAG_G

Cuts at:      0     132    5298 
   Size:        132    5166
 
BanII G_rGCy'C

Cuts at:      0     895    1582    4637    5298 
   Size:        895     687    3055     661

  Fragments arranged by size: 

                3055     895     687     661
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1778    4412    5298 
   Size:       1778    2634     886

  Fragments arranged by size: 

                2634    1778     886
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3873    4420    5298 
   Size:       3873     547     878

  Fragments arranged by size: 

                3873     878     547
 
BclI T'GATC_A

Cuts at:      0     345    1305    4245    5298 
   Size:        345     960    2940    1053

  Fragments arranged by size: 

                2940    1053     960     345
 
BfrBI ATG'CAT

Cuts at:      0     505    5298 
   Size:        505    4793
 
BglII A'GATC_T

Cuts at:      0    1487    3468    5298 
   Size:       1487    1981    1830

  Fragments arranged by size: 

                1981    1830    1487
 
BlpI GC'TnA_GC

Cuts at:      0     816    2496    5091    5298 
   Size:        816    1680    2595     207

  Fragments arranged by size: 

                2595    1680     816     207
 
BmgBI CAC'GTC

Cuts at:      0    1160    5298 
   Size:       1160    4138
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    3661    4981    5298 
   Size:       3661    1320     317

  Fragments arranged by size: 

                3661    1320     317
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     852    2894    5298 
   Size:        852    2042    2404

  Fragments arranged by size: 

                2404    2042     852
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     760    4120    4621    5298 
   Size:        760    3360     501     677

  Fragments arranged by size: 

                3360     760     677     501
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3088    5298 
   Size:       3088    2210
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3269    5298 
   Size:       3269    2029
 
BsaAI yAC'GTr

Cuts at:      0    3179    3193    5298 
   Size:       3179      14    2105

  Fragments arranged by size: 

                3179    2105      14
 
BsaBI GATnn'nnATC

Cuts at:      0    2810    5298 
   Size:       2810    2488
 
BsaWI w'CCGG_w

Cuts at:      0    2066    3674    5298 
   Size:       2066    1608    1624

  Fragments arranged by size: 

                2066    1624    1608
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    5000    5030    5298 
   Size:       5000      30     268

  Fragments arranged by size: 

                5000     268      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     757    1739    4159    4645    5298 
   Size:        757     982    2420     486     653

  Fragments arranged by size: 

                2420     982     757     653     486
 
BseYI C'CCAG_C

Cuts at:      0     939    1171    1694    2983    5298 
   Size:        939     232     523    1289    2315

  Fragments arranged by size: 

                2315    1289     939     523     232
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1996    5298 
   Size:       1996    3302
 
BsiHKAI G_wGCw'C

Cuts at:      0    1582    2064    2565    3432    3748    4637    5298 
   Size:       1582     482     501     867     316     889     661

  Fragments arranged by size: 

                1582     889     867     661     501     482     316
 
BsmI GAATG_Cn'

Cuts at:      0     854    1246    4023    4328    5298 
   Size:        854     392    2777     305     970

  Fragments arranged by size: 

                2777     970     854     392     305
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1166    2360    5281    5298 
   Size:       1166    1194    2921      17

  Fragments arranged by size: 

                2921    1194    1166      17
 
Bsp1286I G_dGCh'C

Cuts at:      0     895    1582    2064    2565    3432    3748    4637    5298 
   Size:        895     687     482     501     867     316     889     661

  Fragments arranged by size: 

                 895     889     867     687     661     501     482     316
            
 
BspHI T'CATG_A

Cuts at:      0     348    2400    5298 
   Size:        348    2052    2898

  Fragments arranged by size: 

                2898    2052     348
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    3450    5298 
   Size:       3450    1848
 
BsrDI GCAATG_nn'

Cuts at:      0    4028    5298 
   Size:       4028    1270
 
BsrGI T'GTAC_A

Cuts at:      0     271    1956    2054    4839    5237    5298 
   Size:        271    1685      98    2785     398      61

  Fragments arranged by size: 

                2785    1685     398     271      98      61
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     502    5298 
   Size:        502    4796
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     947    3876    5071    5298 
   Size:        947    2929    1195     227

  Fragments arranged by size: 

                2929    1195     947     227
 
BstYI r'GATC_y

Cuts at:      0    1487    3468    5298 
   Size:       1487    1981    1830

  Fragments arranged by size: 

                1981    1830    1487
 
BstZ17I GTA'TAC

Cuts at:      0    3439    5298 
   Size:       3439    1859
 
Bsu36I CC'TnA_GG

Cuts at:      0      37    5298 
   Size:         37    5261
 
BtgI C'CryG_G

Cuts at:      0    4730    5298 
   Size:       4730     568
 
BtsI GCAGTG_nn'

Cuts at:      0    2451    2896    3097    3109    4761    4815    5029    5298 
   Size:       2451     445     201      12    1652      54     214     269

  Fragments arranged by size: 

                2451    1652     445     269     214     201      54      12
            
 
DraI TTT'AAA

Cuts at:      0    1890    5298 
   Size:       1890    3408
 
DraIII CAC_nnn'GTG

Cuts at:      0     619    3260    4402    5298 
   Size:        619    2641    1142     896

  Fragments arranged by size: 

                2641    1142     896     619
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     358     931    5298 
   Size:        358     573    4367

  Fragments arranged by size: 

                4367     573     358
 
EaeI y'GGCC_r

Cuts at:      0      54     316    5298 
   Size:         54     262    4982

  Fragments arranged by size: 

                4982     262      54
 
EarI CTCTTCn'nnn_

Cuts at:      0    1209    1274    1570    2147    2321    2885    4177    4277 
   Size:       1209      65     296     577     174     564    1292     100

Cuts at:   4277    4325    5298 
   Size:         48     973

  Fragments arranged by size: 

                1292    1209     973     577     564     296     174     100
                  65      48
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    5043    5298 
   Size:       5043     255
 
EcoICRI GAG'CTC

Cuts at:      0    1580    4635    5298 
   Size:       1580    3055     663

  Fragments arranged by size: 

                3055    1580     663
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     560     760    1190    1549    1655    1718    2270 
   Size:        182     378     200     430     359     106      63     552

Cuts at:   2270    2549    2728    2984    3380    4120    4158    4621    5298 
   Size:        279     179     256     396     740      38     463     677

  Fragments arranged by size: 

                 740     677     552     463     430     396     378     359
                 279     256     200     182     179     106      63      38
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1555    5298 
   Size:       1555    3743
 
EcoO109I rG'GnC_Cy

Cuts at:      0    4596    4608    5298 
   Size:       4596      12     690

  Fragments arranged by size: 

                4596     690      12
 
EcoRI G'AATT_C

Cuts at:      0    2136    5298 
   Size:       2136    3162
 
EcoRV GAT'ATC

Cuts at:      0    2217    2739    5298 
   Size:       2217     522    2559

  Fragments arranged by size: 

                2559    2217     522
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     322    2142    5298 
   Size:        322    1820    3156

  Fragments arranged by size: 

                3156    1820     322
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     865     897    1513    1545    2257    2289    4418    4450 
   Size:        865      32     616      32     712      32    2129      32

Cuts at:   4450    5298 
   Size:        848

  Fragments arranged by size: 

                2129     865     848     712     616      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1181    2238    5298 
   Size:       1181    1057    3060

  Fragments arranged by size: 

                3060    1181    1057
 
HindIII A'AGCT_T

Cuts at:      0     380    1951    2322    5298 
   Size:        380    1571     371    2976

  Fragments arranged by size: 

                2976    1571     380     371
 
MfeI C'AATT_G

Cuts at:      0     929    5298 
   Size:        929    4369
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1115    3743    4353    4594    5298 
   Size:       1115    2628     610     241     704

  Fragments arranged by size: 

                2628    1115     704     610     241
 
MscI TGG'CCA

Cuts at:      0     318    5298 
   Size:        318    4980
 
MslI CAynn'nnrTG

Cuts at:      0    1001    3102    5069    5298 
   Size:       1001    2101    1967     229

  Fragments arranged by size: 

                2101    1967    1001     229
 
MspA1I CmG'CkG

Cuts at:      0     484    3225    3830    4641    5298 
   Size:        484    2741     605     811     657

  Fragments arranged by size: 

                2741     811     657     605     484
 
NcoI C'CATG_G

Cuts at:      0    4730    5298 
   Size:       4730     568
 
NdeI CA'TA_TG

Cuts at:      0    4208    4706    5298 
   Size:       4208     498     592

  Fragments arranged by size: 

                4208     592     498
 
NsiI A_TGCA'T

Cuts at:      0     507    5298 
   Size:        507    4791
 
NspI r_CATG'y

Cuts at:      0    2168    2188    2344    5210    5298 
   Size:       2168      20     156    2866      88

  Fragments arranged by size: 

                2866    2168     156      88      20
 
PciI A'CATG_T

Cuts at:      0    2340    5298 
   Size:       2340    2958
 
PfoI T'CCnGG_A

Cuts at:      0    3583    5298 
   Size:       3583    1715
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1280    1312    1703    1735    5000    5032    5298 
   Size:       1280      32     391      32    3265      32     266

  Fragments arranged by size: 

                3265    1280     391     266      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    4596    4608    5298 
   Size:       4596      12     690

  Fragments arranged by size: 

                4596     690      12
 
PsiI TTA'TAA

Cuts at:      0     595    1255    3329    4945    5298 
   Size:        595     660    2074    1616     353

  Fragments arranged by size: 

                2074    1616     660     595     353
 
PspXI vC'TCGA_Gb

Cuts at:      0    4883    5298 
   Size:       4883     415
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     255     287    4931    4963    5298 
   Size:        255      32    4644      32     335

  Fragments arranged by size: 

                4644     335     255      32      32
 
PstI C_TGCA'G

Cuts at:      0      66     584    3104    5045    5298 
   Size:         66     518    2520    1941     253

  Fragments arranged by size: 

                2520    1941     518     253      66
 
PvuII CAG'CTG

Cuts at:      0     484    3225    3830    4641    5298 
   Size:        484    2741     605     811     657

  Fragments arranged by size: 

                2741     811     657     605     484
 
SacI G_AGCT'C

Cuts at:      0    1582    4637    5298 
   Size:       1582    3055     661

  Fragments arranged by size: 

                3055    1582     661
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1209    1570    4277    5298 
   Size:       1209     361    2707    1021

  Fragments arranged by size: 

                2707    1209    1021     361
 
SfcI C'TryA_G

Cuts at:      0      62     580    3100    5041    5199    5298 
   Size:         62     518    2520    1941     158      99

  Fragments arranged by size: 

                2520    1941     518     158      99      62
 
SmlI C'TyrA_G

Cuts at:      0    2146    3067    4883    4895    5298 
   Size:       2146     921    1816      12     403

  Fragments arranged by size: 

                2146    1816     921     403      12
 
SnaBI TAC'GTA

Cuts at:      0    3179    5298 
   Size:       3179    2119
 
SspI AAT'ATT

Cuts at:      0    3174    3566    4776    5298 
   Size:       3174     392    1210     522

  Fragments arranged by size: 

                3174    1210     522     392
 
StuI AGG'CCT

Cuts at:      0      42    1739    4185    5298 
   Size:         42    1697    2446    1113

  Fragments arranged by size: 

                2446    1697    1113      42
 
StyI C'CwwG_G

Cuts at:      0     132    2117    2200    2220    3936    4730    5298 
   Size:        132    1985      83      20    1716     794     568

  Fragments arranged by size: 

                1985    1716     794     568     132      83      20
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1528    3643    5077    5298 
   Size:       1528    2115    1434     221

  Fragments arranged by size: 

                2115    1528    1434     221
 
TatI w'GTAC_w

Cuts at:      0     271     957    1956    2054    2995    3195    4839    4849 
   Size:        271     686     999      98     941     200    1644      10

Cuts at:   4849    5237    5298 
   Size:        388      61

  Fragments arranged by size: 

                1644     999     941     686     388     271     200      98
                  61      10
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    4740    4772    5298 
   Size:       4740      32     526

  Fragments arranged by size: 

                4740     526      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1777    5298 
   Size:       1777    3521
 
XbaI T'CTAG_A

Cuts at:      0    2793    5298 
   Size:       2793    2505
 
XhoI C'TCGA_G

Cuts at:      0    4883    4895    5298 
   Size:       4883      12     403

  Fragments arranged by size: 

                4883     403      12
 
XmnI GAAnn'nnTTC

Cuts at:      0    3158    3300    5218    5298 
   Size:       3158     142    1918      80

  Fragments arranged by size: 

                3158    1918     142      80
 

 Enzymes that do cut:

     AccI     AclI     AcuI    AflII   AflIII     AleI     AlfI     AloI
    AlwNI     ApoI     AseI     AvaI    AvrII    BanII     BbsI    BciVI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrDI
    BsrGI   BstAPI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI
     DraI   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI    Hin4I   HincII  HindIII
     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NsiI
     NspI     PciI     PfoI     PpiI    PpuMI     PsiI    PspXI     PsrI
     PstI    PvuII     SacI     SapI     SfcI     SmlI    SnaBI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI     AgeI     AhdI     ApaI    ApaLI
     AscI    AsiSI     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
     BglI Bme1580I     BmtI   Bpu10I    BsaHI    BsiEI    BsiWI    BspEI
    BsrBI    BsrFI   BssHII    BssSI    BstBI   BstEII    BtgZI     ClaI
    CspCI     EagI     FseI     FspI    FspAI    HaeII     HpaI     KasI
     KpnI     MluI     NaeI     NarI   NgoMIV     NheI     NotI     NruI
     PacI     PasI    PflMI     PmeI     PmlI    PshAI   PspOMI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SpeI     SphI     SrfI     SwaI
     XcmI     XmaI     ZraI
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