Restriction map for pF1KA0606
##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AgeI        
ApaI        AscI        AseI        AsnI        Asp718I     
AvaI        BciVI       BsaBI       BsiEI       BsiWI       
BsmI        BsoBI       Bsp1407I    BspDI       BsrBI       
BsrGI       BssHII      Bst1107I    BstEII      BstPI       
BstZ17I     ClaI        CpoI        DrdI        EagI        
EclXI       Eco52I      EcoO65I     EcoRI       EcoRV       
FseI        KpnI        MamI        MfeI        MluI        
MunI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        PacI        PaeR7I      
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SalI        SbfI        ScaI        
SexAI       SfiI        SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        SwaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AcsI          775  GAATTT               [GA]^AATT[CT]
AfaI         2672  GTAC                 GT^AC
AflII         336  CTTAAG               C^TTAAG
Alw44I       2130  GTGCAC               G^TGCAC
ApaLI        2130  GTGCAC               G^TGCAC
ApoI          775  GAATTT               [GA]^AATT[CT]
AspI         2469  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        2630  TGCGCA               TGC^GCA
AvrII        2574  CCTAGG               C^CTAGG
BalI         1236  TGGCCA               TGG^CCA
BbeI         3320  GGCGCC               GGCGC^C
BbrPI        2530  CACGTG               CAC^GTG
BbsI         3130  GTCTTC               GAAGAC(2/6)
BbvCI        3544  CCTCAGC              CCTCAGC(-5/-2)
BcgI         2035  GCAGCCCTGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI          336  CTTAAG               C^TTAAG
BglII        2380  AGATCT               A^GATCT
BlnI         2574  CCTAGG               C^CTAGG
BpuAI        3130  GTCTTC               GAAGAC(2/6)
BsaAI        2530  CACGTG               [CT]AC^GT[GA]
BsmBI        1958  GAGACG               CGTCTC(1/5)
BspLU11I      894  ACATGT               A^CATGT
BsrDI         632  GCAATG               GCAATG(2/0)
BsrFI        2770  GCCGGT               [GA]^CCGG[CT]
BssSI         396  CTCGTG               CACGAG(-5/-1)
BstBI        3398  TTCGAA               TT^CGAA
BstXI        1975  CCAGCTGTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I       2770  GCCGGT               [GA]^CCGG[CT]
DraI          462  TTTAAA               TTT^AAA
DraII         160  GGGACCT              [GA]G^G[ACGT]CC[CT]
EaeI         1236  TGGCCA               [CT]^GGCC[GA]
EcoO109I      160  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT22I       208  ATGCAT               ATGCA^T
FspI         2630  TGCGCA               TGC^GCA
HpaI         1557  GTTAAC               GTT^AAC
KasI         3320  GGCGCC               G^GCGCC
KspI         3565  CCGCGG               CCGC^GG
MluNI        1236  TGGCCA               TGG^CCA
MscI         1236  TGGCCA               TGG^CCA
NarI         3320  GGCGCC               GG^CGCC
NsiI          208  ATGCAT               ATGCA^T
NspV         3398  TTCGAA               TT^CGAA
PflFI        2469  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         220  CCACTAATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        2530  CACGTG               CAC^GTG
PmlI         2530  CACGTG               CAC^GTG
PpuMI         160  GGGACCT              [GA]G^G[AT]CC[CT]
RsaI         2672  GTAC                 GT^AC
SacII        3565  CCGCGG               CCGC^GG
SfoI         3320  GGCGCC               GGC^GCC
SfuI         3398  TTCGAA               TT^CGAA
SgrAI        2769  CGCCGGTG             C[GA]^CCGG[CT]G
Tth111I      2469  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        220  CCACTAATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          999  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccIII       1289  TCCGGA               T^CCGGA
             1966  TCCGGA              
AflIII        623  ACGTGT               A^C[GA][CT]GT
              894  ACATGT              
AhdI          140  GACGCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2596  GACAGCCTGTC         
AlwNI        2745  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             3205  CAGCACCTG           
AocI          477  CCTAAGG              CC^T[ACGT]AGG
              855  CCTTAGG             
AspEI         140  GACGCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2596  GACAGCCTGTC         
BglI         1329  GCCTGAAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2770  GCCGGTGGGGC         
BlpI         2763  GCTCAGC              GC^T[ACGT]AGC
             3430  GCTCAGC             
Bpu1102I     2763  GCTCAGC              GC^T[ACGT]AGC
             3430  GCTCAGC             
BsaI          850  GAGACC               GGTCTC(1/5)
             2096  GAGACC              
BseAI        1289  TCCGGA               T^CCGGA
             1966  TCCGGA              
BsgI         2533  GTGCAG               GTGCAG(16/14)
             2722  GTGCAG              
BspEI        1289  TCCGGA               T^CCGGA
             1966  TCCGGA              
Bsu36I        477  CCTAAGG              CC^T[ACGT]AGG
              855  CCTTAGG             
CelII        2763  GCTCAGC              GC^T[ACGT]AGC
             3430  GCTCAGC             
DraIII       1720  CACACCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3364  CACAATGTG           
DsaI         2618  CCGTGG               C^C[GA][CT]GG
             3565  CCGCGG              
Eam1105I      140  GACGCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2596  GACAGCCTGTC         
Eco81I        477  CCTAAGG              CC^T[ACGT]AGG
              855  CCTTAGG             
EcoNI        1948  CCTTCTACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3210  CCTGCTTCAGG         
HincII       1557  GTTAAC               GT[CT]^[GA]AC
             2937  GTCAAC              
HindII       1557  GTTAAC               GT[CT]^[GA]AC
             2937  GTCAAC              
MroI         1289  TCCGGA               T^CCGGA
             1966  TCCGGA              
MslI         2205  CATTGTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2506  CATCCCAGTG          
SapI         2155  GAAGAGC              GCTCTTC(1/4)
             2404  GAAGAGC             
SmlI          336  CTTAAG               C^T[CT][GA]AG
             1387  CTTGAG              
SphI         1715  GCATGC               GCATG^C
             3011  GCATGC              


#########################################
# 3 OR MORE CUT ENZYMES (  103 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
Aor51HI     Asp700I     AspHI       AvaII       BamHI       
BanI        BanII       BbvI        BclI        BcnI        
BfaI        BmyI        BpmI        BsaHI       BsaJI       
BsaWI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspHI       BspMI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       EarI        Eco47III    EcoRII      
EcoT14I     FauI        FbaI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PstI        PvuII       RcaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII       XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com