Restriction map for pF1KA0599
##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

AclI        AcsI        AflIII      Alw44I      Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     AspI        AvrII       BamHI       BciVI       
BclI        BlnI        BsiWI       Bsp1407I    BspDI       
BspHI       BspLU11I    BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
ClaI        DraI        DrdI        Eco47III    EcoO65I     
EcoRI       FbaI        HindIII     HpaI        MfeI        
MluI        MunI        NdeI        NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        SalI        SbfI        SexAI       SfuI        
SpeI        Sse8387I    SspBI       SspI        SwaI        
Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AatII        3508  GACGTC               GACGT^C
Acc65I       1256  GGTACC               G^GTACC
AflII        2077  CTTAAG               C^TTAAG
AhdI         2230  GACAGCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI         3170  GGATC                GGATC(4/5)
AocI         1894  CCTGAGG              CC^T[ACGT]AGG
Asp718I      1256  GGTACC               G^GTACC
AspEI        2230  GACAGCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI         1331  GGCGCC               GGCGC^C
BbrPI        3112  CACGTG               CAC^GTG
BcgI         1847  GCATGAGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         2077  CTTAAG               C^TTAAG
BglII        2810  AGATCT               A^GATCT
BsaBI        1044  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        1951  GAGACG               CGTCTC(1/5)
BsmI          586  GAATGC               GAATGC(1/-1)
BstYI        2810  AGATCT               [GA]^GATC[CT]
Bsu36I       1894  CCTGAGG              CC^T[ACGT]AGG
CpoI          836  CGGTCCG              CG^G[AT]CCG
DraIII       3601  CACTCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     2230  GACAGCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1894  CCTGAGG              CC^T[ACGT]AGG
EcoRV         532  GATATC               GAT^ATC
EcoT22I       588  ATGCAT               ATGCA^T
HincII       3589  GTCAAC               GT[CT]^[GA]AC
HindII       3589  GTCAAC               GT[CT]^[GA]AC
KasI         1331  GGCGCC               G^GCGCC
KpnI         1256  GGTACC               GGTAC^C
MamI         1044  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         2810  AGATCT               [GA]^GATC[CT]
NarI         1331  GGCGCC               GG^CGCC
NcoI          104  CCATGG               C^CATGG
NsiI          588  ATGCAT               ATGCA^T
PmaCI        3112  CACGTG               CAC^GTG
PmlI         3112  CACGTG               CAC^GTG
RsrII         836  CGGTCCG              CG^G[AT]CCG
ScaI         1834  AGTACT               AGT^ACT
SfiI         2994  GGCCCACCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1331  GGCGCC               GGC^GCC
SgrAI        3869  CACCGGTG             C[GA]^CCGG[CT]G
SnaBI         322  TACGTA               TAC^GTA
XhoII        2810  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccIII       2833  TCCGGA               T^CCGGA
             3167  TCCGGA              
AgeI         2456  ACCGGT               A^CCGGT
             3870  ACCGGT              
AviII         335  TGCGCA               TGC^GCA
              941  TGCGCA              
BbsI         2460  GTCTTC               GAAGAC(2/6)
             2829  GTCTTC              
BbvCI        2343  CCTCAGC              CCTCAGC(-5/-2)
             3477  CCTCAGC             
BfaI         1180  CTAG                 C^TAG
             2512  CTAG                
BpuAI        2460  GTCTTC               GAAGAC(2/6)
             2829  GTCTTC              
BseAI        2833  TCCGGA               T^CCGGA
             3167  TCCGGA              
BspEI        2833  TCCGGA               T^CCGGA
             3167  TCCGGA              
BsrBI          40  GAGCGG               CCGCTC(-3/-3)
             1273  GAGCGG              
BsrDI        2175  CATTGC               GCAATG(2/0)
              944  GCAATG              
EcoNI          18  CCTCCACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1872  CCTGCCAGAGG         
FseI         3454  GGCCGGCC             GGCCGG^CC
             3568  GGCCGGCC            
FspI          335  TGCGCA               TGC^GCA
              941  TGCGCA              
KspI         2257  CCGCGG               CCGC^GG
             3576  CCGCGG              
MaeI         1180  CTAG                 C^TAG
             2512  CTAG                
MroI         2833  TCCGGA               T^CCGGA
             3167  TCCGGA              
MseI         2078  TTAA                 T^TAA
             2522  TTAA                
PinAI        2456  ACCGGT               A^CCGGT
             3870  ACCGGT              
SacI         2725  GAGCTC               GAGCT^C
             3517  GAGCTC              
SacII        2257  CCGCGG               CCGC^GG
             3576  CCGCGG              
SapI         1546  GAAGAGC              GCTCTTC(1/4)
             3243  GAAGAGC             
SmlI          694  CTCAAG               C^T[CT][GA]AG
             2077  CTTAAG              
SphI          590  GCATGC               GCATG^C
             1404  GCATGC              
StuI         1553  AGGCCT               AGG^CCT
             4001  AGGCCT              
TaqI         1052  TCGA                 T^CGA
             1856  TCGA                
Tru9I        2078  TTAA                 T^TAA
             2522  TTAA                
TthHB8I      1052  TCGA                 T^CGA
             1856  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (  119 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AluI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbvI        
BcnI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BsaAI       BsaHI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EagI        EarI        EclXI       Eco52I      EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        StyI        
TfiI        TseI        Tsp45I      Tsp509I     TspRI       
Van91I      XcmI        XmaCI       XmaI        
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