Restriction map for pF1KA0598
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# NO CUT ENZYMES (  100 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflIII      
AgeI        Alw44I      ApaLI       AscI        Asp718I     
AspI        BalI        BamHI       BanI        BbeI        
BbvCI       BcgI        BciVI       BclI        BglII       
BsaBI       BseAI       BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrFI       BsrGI       BssSI       
Bst1107I    BstZ17I     Cfr10I      ClaI        CpoI        
DraI        DrdI        EagI        EarI        EclXI       
Eco52I      EcoRI       EcoRV       FbaI        FseI        
HindIII     HpaI        KasI        KpnI        Ksp632I     
MamI        MfeI        MluI        MluNI       MroI        
MscI        MunI        NaeI        NarI        NcoI        
NgoMIV      NheI        NotI        NruI        PacI        
PaeR7I      PflFI       PinAI       PleI        PmeI        
PshAI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SalI        SapI        SbfI        ScaI        
SexAI       SfoI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SwaI        Tth111I     
XbaI        XcmI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   59 enzymes) #
###################################

AccI         1315  GTCTAC               GT^[AC][GT]AC
AhdI         1157  GACTGATTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          969  GATCC                GGATC(4/5)
AlwNI        1480  CAGTTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          829  CCTGAGG              CC^T[ACGT]AGG
Asp700I       735  GAAGCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1157  GACTGATTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         655  GTGCTC               G[AT]GC[AT]^C
AviII         695  TGCGCA               TGC^GCA
AvrII        1491  CCTAGG               C^CTAGG
BbrPI         105  CACGTG               CAC^GTG
BcnI          872  CCCGG                CC^[GC]GG
BlnI         1491  CCTAGG               C^CTAGG
BlpI         1392  GCTCAGC              GC^T[ACGT]AGC
BpmI         1354  CTCCAG               CTGGAG(16/14)
Bpu1102I     1392  GCTCAGC              GC^T[ACGT]AGC
BsaI          914  GAGACC               GGTCTC(1/5)
BsaWI        1565  ACCGGA               [AT]^CCGG[AT]
BseRI         604  GAGGAG               GAGGAG(10/8)
BsgI          823  CTGCAC               GTGCAG(16/14)
BsiHKAI       655  GTGCTC               G[AT]GC[AT]^C
BsmAI         914  GAGAC                GTCTC(1/5)
BsrBI         675  CCGCTC               CCGCTC(-3/-3)
BssHII       1305  GCGCGC               G^CGCGC
BstBI        1221  TTCGAA               TT^CGAA
BstEII         88  GGTCACC              G^GT[ACGT]ACC
BstPI          88  GGTCACC              G^GT[ACGT]ACC
BstYI         968  AGATCC               [GA]^GATC[CT]
Bsu36I        829  CCTGAGG              CC^T[ACGT]AGG
CelII        1392  GCTCAGC              GC^T[ACGT]AGC
DraIII       1050  CACGATGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          956  TGGCCG               [CT]^GGCC[GA]
Eam1105I     1157  GACTGATTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        829  CCTGAGG              CC^T[ACGT]AGG
EcoNI         693  CCTGCGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I        88  GGTCACC              G^GT[ACGT]ACC
EcoT22I      1442  ATGCAT               ATGCA^T
FspI          695  TGCGCA               TGC^GCA
HincII         61  GTCAAC               GT[CT]^[GA]AC
HindII         61  GTCAAC               GT[CT]^[GA]AC
KspI         1232  CCGCGG               CCGC^GG
MflI          968  AGATCC               [GA]^GATC[CT]
NciI          872  CCCGG                CC^[GC]GG
NdeI          544  CATATG               CA^TATG
NsiI         1442  ATGCAT               ATGCA^T
NspI         1286  GCATGC               [GA]CATG^[CT]
NspV         1221  TTCGAA               TT^CGAA
PflMI        1049  CCACGATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         105  CACGTG               CAC^GTG
PmlI          105  CACGTG               CAC^GTG
SacII        1232  CCGCGG               CCGC^GG
SfiI         1577  GGCCCATGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI         1221  TTCGAA               TT^CGAA
SphI         1286  GCATGC               GCATG^C
SspI          424  AATATT               AAT^ATT
StuI          701  AGGCCT               AGG^CCT
Van91I       1049  CCACGATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         968  AGATCC               [GA]^GATC[CT]
XmnI          735  GAAGCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcsI          125  AAATTC               [GA]^AATT[CT]
              568  AAATTC              
AcyI          688  GACGCC               G[GA]^CG[CT]C
             1553  GGCGTC              
AflII         526  CTTAAG               C^TTAAG
             1500  CTTAAG              
Aor51HI       668  AGCGCT               AGC^GCT
              878  AGCGCT              
ApoI          125  AAATTC               [GA]^AATT[CT]
              568  AAATTC              
AseI           13  ATTAAT               AT^TAAT
              459  ATTAAT              
AsnI           13  ATTAAT               AT^TAAT
              459  ATTAAT              
AvaI          612  CTCGGG               C^[CT]CG[GA]G
             1558  CCCGAG              
BfrI          526  CTTAAG               C^TTAAG
             1500  CTTAAG              
BsaAI         105  CACGTG               [CT]AC^GT[GA]
              652  TACGTG              
BsaHI         688  GACGCC               G[GA]^CG[CT]C
             1553  GGCGTC              
BsoBI         612  CTCGGG               C^[CT]CG[GA]G
             1558  CCCGAG              
BspMI          57  GCAGGT               ACCTGC(4/8)
             1644  GCAGGT              
BstXI         359  CCAGCTTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              859  CCACACTGGTGG        
DsaI          926  CCGTGG               C^C[GA][CT]GG
             1232  CCGCGG              
Eco47III      668  AGCGCT               AGC^GCT
              878  AGCGCT              
HaeII         668  AGCGCT               [GA]GCGC^[CT]
              878  AGCGCT              
HapII         873  CCGG                 C^CGG
             1566  CCGG                
Hin1I         688  GACGCC               G[GA]^CG[CT]C
             1553  GGCGTC              
HinfI         887  GAATC                G^A[ACGT]TC
             1602  GATTC               
HpaII         873  CCGG                 C^CGG
             1566  CCGG                
MslI          860  CACACTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1341  CATGCCTGTG          
MspI          873  CCGG                 C^CGG
             1566  CCGG                
PpuMI        1175  GGGACCC              [GA]G^G[AT]CC[CT]
             1646  AGGTCCT             
PshBI          13  ATTAAT               AT^TAAT
              459  ATTAAT              
PstI          982  CTGCAG               CTGCA^G
             1267  CTGCAG              
PvuII          28  CAGCTG               CAG^CTG
             1270  CAGCTG              
SmlI          526  CTTAAG               C^T[CT][GA]AG
             1500  CTTAAG              
TfiI          887  GAATC                G^A[AT]TC
             1602  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        ApaI        
AvaII       BanII       BbsI        BbvI        BfaI        
BglI        BmyI        BpuAI       BsaJI       BsiYI       
BslI        BsmFI       Bsp1286I    BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HgaI        HhaI        HinP1I      
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MspA1I      
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TaqI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
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