Restriction map for pF1KA0542
##################################
# NO CUT ENZYMES (   77 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        Alw44I      ApaLI       AseI        AsnI        
Asp718I     AspEI       BciVI       BclI        BfrI        
BglII       BsaBI       BsiEI       BsiWI       BsmBI       
BsmI        BspDI       BspLU11I    BstEII      BstPI       
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco52I      EcoO65I     EcoRV       
EcoT22I     FbaI        FseI        HincII      HindII      
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MunI        NaeI        NgoMIV      NheI        
NotI        NruI        NsiI        PacI        PaeR7I      
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
SfiI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspI        SwaI        XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI         1293  GTATAC               GT^[AC][GT]AC
AccIII        173  TCCGGA               T^CCGGA
AcsI         1738  GAATTC               [GA]^AATT[CT]
AflIII       1438  ACACGT               A^C[GA][CT]GT
AocI         3484  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      2183  AGCGCT               AGC^GCT
ApoI         1738  GAATTC               [GA]^AATT[CT]
AscI         1058  GGCGCGCC             GG^CGCGCC
AspI          135  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1997  CCCGAG               C^[CT]CG[GA]G
BamHI         170  GGATCC               G^GATCC
BglI         3307  GCCACCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI         173  TCCGGA               [AT]^CCGG[AT]
BseAI         173  TCCGGA               T^CCGGA
BsoBI        1997  CCCGAG               C^[CT]CG[GA]G
Bsp1407I      458  TGTACA               T^GTACA
BspEI         173  TCCGGA               T^CCGGA
BspHI         540  TCATGA               T^CATGA
BsrDI        2793  CATTGC               GCAATG(2/0)
BsrGI         458  TGTACA               T^GTACA
BssHII       1059  GCGCGC               G^CGCGC
BssSI         409  CACGAG               CACGAG(-5/-1)
Bst1107I     1293  GTATAC               GTA^TAC
BstZ17I      1293  GTATAC               GTA^TAC
Bsu36I       3484  CCTGAGG              CC^T[ACGT]AGG
DraIII       1849  CACAGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     2183  AGCGCT               AGC^GCT
Eco81I       3484  CCTGAGG              CC^T[ACGT]AGG
EcoRI        1738  GAATTC               G^AATTC
HindIII      1667  AAGCTT               A^AGCTT
MroI          173  TCCGGA               T^CCGGA
NcoI         1991  CCATGG               C^CATGG
NdeI         2052  CATATG               CA^TATG
PflFI         135  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI          540  TCATGA               T^CATGA
SapI         1129  GAAGAGC              GCTCTTC(1/4)
ScaI         2032  AGTACT               AGT^ACT
SexAI        2173  ACCTGGT              A^CC[AT]GGT
SphI         1230  GCATGC               GCATG^C
SspBI         458  TGTACA               T^GTACA
Tth111I       135  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1991  CCATGGCCCGAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

Asp700I       366  GAAGGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1665  GAAAGCTTTC          
AvrII        3000  CCTAGG               C^CTAGG
             3229  CCTAGG              
BbeI         1403  GGCGCC               GGCGC^C
             3589  GGCGCC              
BbrPI         675  CACGTG               CAC^GTG
             2481  CACGTG              
BbvCI        2728  CCTCAGC              CCTCAGC(-5/-2)
             2878  GCTGAGG             
BcgI         3437  CGAGCAGCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1268  GCAAATGTATCG        
BlnI         3000  CCTAGG               C^CTAGG
             3229  CCTAGG              
BsaI         2824  GAGACC               GGTCTC(1/5)
             1184  GGTCTC              
BsrFI         242  GCCGGT               [GA]^CCGG[CT]
             2255  ACCGGC              
BstBI         299  TTCGAA               TT^CGAA
              373  TTCGAA              
Cfr10I        242  GCCGGT               [GA]^CCGG[CT]
             2255  ACCGGC              
DsaI         1650  CCACGG               C^C[GA][CT]GG
             1991  CCATGG              
KasI         1403  GGCGCC               G^GCGCC
             3589  GGCGCC              
MslI          964  CACTTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1259  CACTGCTGTG          
NarI         1403  GGCGCC               GG^CGCC
             3589  GGCGCC              
NspI         1085  ACATGC               [GA]CATG^[CT]
             1230  GCATGC              
NspV          299  TTCGAA               TT^CGAA
              373  TTCGAA              
PleI         1391  GACTC                GAGTC(4/5)
             2360  GACTC               
PmaCI         675  CACGTG               CAC^GTG
             2481  CACGTG              
PmlI          675  CACGTG               CAC^GTG
             2481  CACGTG              
PpuMI        2063  AGGTCCT              [GA]G^G[AT]CC[CT]
             2775  GGGTCCC             
SfoI         1403  GGCGCC               GGC^GCC
             3589  GGCGCC              
SfuI          299  TTCGAA               TT^CGAA
              373  TTCGAA              
XmnI          366  GAAGGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1665  GAAAGCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  115 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaII       
AviII       BalI        BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaHI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BsrBI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PflMI       PstI        PvuII       
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com