Restriction map for pF1KA0542
 (Circular) (Six-base) MAPSORT of: pF1KA0542.seq  Check: 1973  from: 1  to: 6746



pF1KA0542  6746 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   3548    3606    3548 
   Size:         58    6688
 
AccI GT'mk_AC

Cuts at:   1402    3784    1402 
   Size:       2382    4364
 
Acc65I G'GTAC_C

Cuts at:   3762    3762 
   Size:       6746
 
AclI AA'CG_TT

Cuts at:   6586    6586 
   Size:       6746
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    706    4571    5003    5750    6018     706 
   Size:       3865     432     747     268    1434

  Fragments arranged by size: 

                3865    1434     747     432     268
 
AfeI AGC'GCT

Cuts at:   2293    2293 
   Size:       6746
 
AflIII A'CryG_T

Cuts at:   1546    4098    5202    6179    1546 
   Size:       2552    1104     977    2113

  Fragments arranged by size: 

                2552    2113    1104     977
 
AgeI A'CCGG_T

Cuts at:   5134    5134 
   Size:       6746
 
AleI CACnn'nnGTG

Cuts at:   1076    1371    1076 
   Size:        295    6451
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1389    2470    1389 
   Size:       1081    5665
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2148    2180    3511    3543    2148 
   Size:         32    1331      32    5351

  Fragments arranged by size: 

                5351    1331      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:    900    1263    1277    1502    1668    1866    1929    2064    2270 
   Size:        363      14     225     166     198      63     135     206

Cuts at:   2270    2331    2373    2733    2844    3592    3660    3723    3735 
   Size:         61      42     360     111     748      68      63      12

Cuts at:   3735    4051    5618     900 
   Size:        316    1567    2028

  Fragments arranged by size: 

                2028    1567     748     363     360     316     225     206
                 198     166     135     111      68      63      63      61
                  42      14      12
 
ApaI G_GGCC'C

Cuts at:    855    2547    3204     855 
   Size:       1692     657    4397

  Fragments arranged by size: 

                4397    1692     657
 
ApaLI G'TGCA_C

Cuts at:   5516    5516 
   Size:       6746
 
ApoI r'AATT_y

Cuts at:   1846    3750    6099    6355    1846 
   Size:       1904    2349     256    2237

  Fragments arranged by size: 

                2349    2237    1904     256
 
AscI GG'CGCG_CC

Cuts at:   1167    1167 
   Size:       6746
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6746
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6746
 
AvaI C'yCGr_G

Cuts at:   2105    3766    2105 
   Size:       1661    5085
 
AvrII C'CTAG_G

Cuts at:   3108    3337    3108 
   Size:        229    6517
 
BamHI G'GATC_C

Cuts at:    278    3771     278 
   Size:       3493    3253
 
BanI G'GyrC_C

Cuts at:   1511    1691    2680    2923    3697    3762    4176    4424    4459 
   Size:        180     989     243     774      65     414     248      35

Cuts at:   4459    1511 
   Size:       3798

  Fragments arranged by size: 

                3798     989     774     414     248     243     180      65
                  35
 
BanII G_rGCy'C

Cuts at:    855    1470    1811    2547    2970    2996    3011    3204    3674 
   Size:        615     341     736     423      26      15     193     470

Cuts at:   3674    3760    4790     855 
   Size:         86    1030    2811

  Fragments arranged by size: 

                2811    1030     736     615     470     423     341     193
                  86      26      15
 
BbeI G_GCGC'C

Cuts at:   1515    3701    4180    4428    1515 
   Size:       2186     479     248    3833

  Fragments arranged by size: 

                3833    2186     479     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    254     471     598    2404     254 
   Size:        217     127    1806    4596

  Fragments arranged by size: 

                4596    1806     217     127
 
BbvCI CC'TCA_GC

Cuts at:   2837    2987    2837 
   Size:        150    6596
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1365    1399    3534    3568    1365 
   Size:         34    2135      34    4543

  Fragments arranged by size: 

                4543    2135      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4644    5405    6746    4644 
   Size:        761    1341    4644

  Fragments arranged by size: 

                4644    1341     761
 
BclI T'GATC_A

Cuts at:   4266    4266 
   Size:       6746
 
BglI GCCn_nnn'nGGC

Cuts at:   3421    3421 
   Size:       6746
 
BglII A'GATC_T

Cuts at:   4261    6298    4261 
   Size:       2037    4709
 
BlpI GC'TnA_GC

Cuts at:   1037    3119    3680    3858    1037 
   Size:       2082     561     178    3925

  Fragments arranged by size: 

                3925    2082     561     178
 
Bme1580I G_kGCm'C

Cuts at:    825     855    1749    1803    2547    3204    4371    4464    5520 
   Size:         30     894      54     744     657    1167      93    1056

Cuts at:   5520     825 
   Size:       2051

  Fragments arranged by size: 

                2051    1167    1056     894     744     657      93      54
                  30
 
BmgBI CAC'GTC

Cuts at:    587    1711     587 
   Size:       1124    5622
 
BmrI ACTGGGnnnn_n'

Cuts at:   1215    3958    4039    4374    6518    1215 
   Size:       2743      81     335    2144    1443

  Fragments arranged by size: 

                2743    2144    1443     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1342    5113    1342 
   Size:       3771    2975
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    763     847    1048    1611    1809    1899    2359    2398    2632 
   Size:         84     201     563     198      90     460      39     234

Cuts at:   2632    2656    3025    3586    3658     763 
   Size:         24     369     561      72    3851

  Fragments arranged by size: 

                3851     563     561     460     369     234     201     198
                  90      84      72      39      24
 
Bpu10I CC'TnA_GC

Cuts at:    856    2837    2987     856 
   Size:       1981     150    4615

  Fragments arranged by size: 

                4615    1981     150
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1753    2950    3481    3493    3922    4801    5293    5591    1753 
   Size:       1197     531      12     429     879     492     298    2908

  Fragments arranged by size: 

                2908    1197     879     531     492     429     298      12
            
 
BsaI GGTCTCn'nnnn_

Cuts at:   1298    2926    6450    1298 
   Size:       1628    3524    1594

  Fragments arranged by size: 

                3524    1628    1594
 
BsaAI yAC'GTr

Cuts at:    699     785    2591    4099     699 
   Size:         86    1806    1508    3346

  Fragments arranged by size: 

                3346    1806    1508      86
 
BsaBI GATnn'nnATC

Cuts at:      6    4260    6297       6 
   Size:       4254    2037     455

  Fragments arranged by size: 

                4254    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1512    3045    3698    4177    4425    5127    6623    1512 
   Size:       1533     653     479     248     702    1496    1635

  Fragments arranged by size: 

                1635    1533    1496     702     653     479     248
 
BsaWI w'CCGG_w

Cuts at:    281    3935    4456    5134    5408    5555    5966     281 
   Size:       3654     521     678     274     147     411    1061

  Fragments arranged by size: 

                3654    1061     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1099    1129    1711    1741    1782    1812    1099 
   Size:         30     582      30      41      30    6033

  Fragments arranged by size: 

                6033     582      41      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    511    2631    2659    3004    3490    3595    3682     511 
   Size:       2120      28     345     486     105      87    3575

  Fragments arranged by size: 

                3575    2120     486     345     105      87      28
 
BseYI C'CCAG_C

Cuts at:   1859    2321    2741    2817    2860    2908    2914    3424    4171 
   Size:        462     420      76      43      48       6     510     747

Cuts at:   4171    4929    5506    6002    1859 
   Size:        758     577     496    2603

  Fragments arranged by size: 

                2603     758     747     577     510     496     462     420
                  76      48      43       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    236     334     889    1226    1252    1930    1951    2224    2419 
   Size:         98     555     337      26     678      21     273     195

Cuts at:   2419    3287    3481    3640     236 
   Size:        868     194     159    3342

  Fragments arranged by size: 

                3342     868     678     555     337     273     195     194
                 159      98      26      21
 
BsiEI CG_ry'CG

Cuts at:    104    4288    4334    5542     104 
   Size:       4184      46    1208    1308

  Fragments arranged by size: 

                4184    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    784    1064    1244    2449    2970    3011    3674    3760    4538 
   Size:        280     180    1205     521      41     663      86     778

Cuts at:   4538    4728    5520     784 
   Size:        190     792    2010

  Fragments arranged by size: 

                2010    1205     792     778     663     521     280     190
                 180      86      41
 
Bsp1286I G_dGCh'C

Cuts at:    784     825     855    1064    1244    1470    1749    1803    1811 
   Size:         41      30     209     180     226     279      54       8

Cuts at:   1811    2449    2547    2970    2996    3011    3204    3674    3760 
   Size:        638      98     423      26      15     193     470      86

Cuts at:   3760    4371    4464    4538    4728    4790    5520     784 
   Size:        611      93      74     190      62     730    2010

  Fragments arranged by size: 

                2010     730     638     611     470     423     279     226
                 209     193     190     180      98      93      86      74
                  62      54      41      30      26      15       8
 
BspEI T'CCGG_A

Cuts at:    281    5966     281 
   Size:       5685    1061
 
BspHI T'CATG_A

Cuts at:    648    5922     648 
   Size:       5274    1472
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1004    1952    2488    2739    3548    3606    3796    4010    4312 
   Size:        948     536     251     809      58     190     214     302

Cuts at:   4312    4693    1004 
   Size:        381    3057

  Fragments arranged by size: 

                3057     948     809     536     381     302     251     214
                 190      58
 
BsrBI CCG'CTC

Cuts at:     50    1101    1444    1915    1920    5038    5092    6576    6741 
   Size:       1051     343     471       5    3118      54    1484     165

Cuts at:   6741      50 
   Size:         55

  Fragments arranged by size: 

                3118    1484    1051     471     343     165      55      54
                   5
 
BsrDI GCAATG_nn'

Cuts at:   2900    4658    2900 
   Size:       1758    4988
 
BsrFI r'CCGG_y

Cuts at:    350    2363    4744    4925    5134     350 
   Size:       2013    2381     181     209    1962

  Fragments arranged by size: 

                2381    2013    1962     209     181
 
BsrGI T'GTAC_A

Cuts at:    566     566 
   Size:       6746
 
BssHII G'CGCG_C

Cuts at:   1167    1167 
   Size:       6746
 
BssSI C'ACGA_G

Cuts at:    517    5017    5375     517 
   Size:       4500     358    1888

  Fragments arranged by size: 

                4500    1888     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    374     666    1962    2064    2331    2373    2409    2733    3495 
   Size:        292    1296     102     267      42      36     324     762

Cuts at:   3495    3618    4123     374 
   Size:        123     505    2997

  Fragments arranged by size: 

                2997    1296     762     505     324     292     267     123
                 102      42      36
 
BstBI TT'CG_AA

Cuts at:    408     482    5107     408 
   Size:         74    4625    2047

  Fragments arranged by size: 

                4625    2047      74
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1155    2175    2595    3694    1155 
   Size:       1020     420    1099    4207

  Fragments arranged by size: 

                4207    1099    1020     420
 
BstYI r'GATC_y

Cuts at:      1     278    3479    3650    3771    4238    4261    4596    4842 
   Size:        277    3201     171     121     467      23     335     246

Cuts at:   4842    5854    5940    5952    6298       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                3201    1012     467     449     346     335     277     246
                 171     121      86      23      12
 
BstZ17I GTA'TAC

Cuts at:   1403    1403 
   Size:       6746
 
Bsu36I CC'TnA_GG

Cuts at:   3593    3593 
   Size:       6746
 
BtgI C'CryG_G

Cuts at:   1758    2099    1758 
   Size:        341    6405
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1521    2838    4696    4877    5040    1521 
   Size:       1317    1858     181     163    3227

  Fragments arranged by size: 

                3227    1858    1317     181     163
 
BtsI GCAGTG_nn'

Cuts at:    944    1366    2354    2419    2644    3014    3989     944 
   Size:        422     988      65     225     370     975    3701

  Fragments arranged by size: 

                3701     988     975     422     370     225      65
 
DraI TTT'AAA

Cuts at:   3745    3745 
   Size:       6746
 
DraIII CAC_nnn'GTG

Cuts at:   1962    1962 
   Size:       6746
 
DrdI GACnn_nn'nnGTC

Cuts at:   4452    5310    4452 
   Size:        858    5888
 
EaeI y'GGCC_r

Cuts at:    380    1145    1640    3368    4331    4505    4896    4923    6268 
   Size:        765     495    1728     963     174     391      27    1345

Cuts at:   6268     380 
   Size:        858

  Fragments arranged by size: 

                1728    1345     963     858     765     495     391     174
                  27
 
EagI C'GGCC_G

Cuts at:   4331    4331 
   Size:       6746
 
EarI CTCTTCn'nnn_

Cuts at:    417    1009    1232    1405    2419    4769    4979     417 
   Size:        592     223     173    1014    2350     210    2184

  Fragments arranged by size: 

                2350    2184    1014     592     223     210     173
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2476    2773    4954    5264    5410    6556    2476 
   Size:        297    2181     310     146    1146    2666

  Fragments arranged by size: 

                2666    2181    1146     310     297     146
 
EcoICRI GAG'CTC

Cuts at:   2968    3009    3672    3758    2968 
   Size:         41     663      86    5956

  Fragments arranged by size: 

                5956     663      86      41
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    706     763     847    1048    1611    1809    1899    2359    2398 
   Size:         57      84     201     563     198      90     460      39

Cuts at:   2398    2632    2656    3025    3586    3658    4571    5003    5750 
   Size:        234      24     369     561      72     913     432     747

Cuts at:   5750    6018     706 
   Size:        268    1434

  Fragments arranged by size: 

                1434     913     747     563     561     460     432     369
                 268     234     201     198      90      84      72      57
                  39      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2023    2674    3157    3727    2023 
   Size:        651     483     570    5042

  Fragments arranged by size: 

                5042     651     570     483
 
EcoO109I rG'GnC_Cy

Cuts at:    851     852    2172    2544    2884    3162    3200    3201    3589 
   Size:          1    1320     372     340     278      38       1     388

Cuts at:   3589    3720    3732    3885     851 
   Size:        131      12     153    3712

  Fragments arranged by size: 

                3712    1320     388     372     340     278     153     131
                  38      12       1       1
 
EcoRI G'AATT_C

Cuts at:   1846    3750    1846 
   Size:       1904    4842
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   4765    4765 
   Size:       6746
 
FspI TGC'GCA

Cuts at:    367    3071    3556    4527     367 
   Size:       2704     485     971    2586

  Fragments arranged by size: 

                2704    2586     971     485
 
HaeII r_GCGC'y

Cuts at:   1515    1978    2295    2647    3215    3687    3701    4180    4428 
   Size:        463     317     352     568     472      14     479     248

Cuts at:   4428    5450    6429    1515 
   Size:       1022     979    1832

  Fragments arranged by size: 

                1832    1022     979     568     479     472     463     352
                 317     248      14
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    723     755    2391    2423    4588    4618    4620    4650     723 
   Size:         32    1636      32    2165      30       2      30    2819

  Fragments arranged by size: 

                2819    2165    1636      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   3785    3785 
   Size:       6746
 
HindIII A'AGCT_T

Cuts at:   1775    1775 
   Size:       6746
 
KasI G'GCGC_C

Cuts at:   1511    3697    4176    4424    1511 
   Size:       2186     479     248    3833

  Fragments arranged by size: 

                3833    2186     479     248
 
KpnI G_GTAC'C

Cuts at:   3766    3766 
   Size:       6746
 
MluI A'CGCG_T

Cuts at:   6179    6179 
   Size:       6746
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    275    3041    5417    5601     275 
   Size:       2766    2376     184    1420

  Fragments arranged by size: 

                2766    2376    1420     184
 
MscI TGG'CCA

Cuts at:    382    1147    1642    3370    4507     382 
   Size:        765     495    1728    1137    2621

  Fragments arranged by size: 

                2621    1728    1137     765     495
 
MslI CAynn'nnrTG

Cuts at:   1076    1371    4735    4862    1076 
   Size:        295    3364     127    2960

  Fragments arranged by size: 

                3364    2960     295     127
 
MspA1I CmG'CkG

Cuts at:   1260    1428    2061    2337    2370    2505    2529    2733    2789 
   Size:        168     633     276      33     135      24     204      56

Cuts at:   2789    3327    3657    3857    4171    4531    5544    5789    1260 
   Size:        538     330     200     314     360    1013     245    2217

  Fragments arranged by size: 

                2217    1013     633     538     360     330     314     276
                 245     204     200     168     135      56      33      24
            
 
NaeI GCC'GGC

Cuts at:   4927    4927 
   Size:       6746
 
NarI GG'CG_CC

Cuts at:   1512    3698    4177    4425    1512 
   Size:       2186     479     248    3833

  Fragments arranged by size: 

                3833    2186     479     248
 
NcoI C'CATG_G

Cuts at:   2099    2099 
   Size:       6746
 
NdeI CA'TA_TG

Cuts at:   2161    2161 
   Size:       6746
 
NgoMIV G'CCGG_C

Cuts at:   4925    4925 
   Size:       6746
 
NspI r_CATG'y

Cuts at:   1197    1342    3799    4299    5206    1197 
   Size:        145    2457     500     907    2737

  Fragments arranged by size: 

                2737    2457     907     500     145
 
PciI A'CATG_T

Cuts at:   5202    5202 
   Size:       6746
 
PflMI CCAn_nnn'nTGG

Cuts at:   2457    2801    3003    3660    2457 
   Size:        344     202     657    5543

  Fragments arranged by size: 

                5543     657     344     202
 
PfoI T'CCnGG_A

Cuts at:   2021    2672    2021 
   Size:        651    6095
 
PmeI GTTT'AAAC

Cuts at:   3745    3745 
   Size:       6746
 
PmlI CAC'GTG

Cuts at:    785    2591     785 
   Size:       1806    4940
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    629     661    5885    5917     629 
   Size:         32    5224      32    1458

  Fragments arranged by size: 

                5224    1458      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2172    2884    2172 
   Size:        712    6034
 
PspOMI G'GGCC_C

Cuts at:    851    2543    3200     851 
   Size:       1692     657    4397

  Fragments arranged by size: 

                4397    1692     657
 
PstI C_TGCA'G

Cuts at:    939    1982    2308    2336    2414    2573    2639    2738    3447 
   Size:       1043     326      28      78     159      66      99     709

Cuts at:   3447    3793    4478     939 
   Size:        346     685    3207

  Fragments arranged by size: 

                3207    1043     709     685     346     326     159      99
                  78      66      28
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6746
 
PvuII CAG'CTG

Cuts at:   1260    1428    2061    2337    2370    2505    2733    3657    4171 
   Size:        168     633     276      33     135     228     924     514

Cuts at:   4171    4531    1260 
   Size:        360    3475

  Fragments arranged by size: 

                3475     924     633     514     360     276     228     168
                 135      33
 
RsrII CG'GwC_CG

Cuts at:   4941    4941 
   Size:       6746
 
SacI G_AGCT'C

Cuts at:   2970    3011    3674    3760    2970 
   Size:         41     663      86    5956

  Fragments arranged by size: 

                5956     663      86      41
 
SalI G'TCGA_C

Cuts at:   3783    3783 
   Size:       6746
 
SanDI GG'GwC_CC

Cuts at:   2884    2884 
   Size:       6746
 
SapI GCTCTTCn'nnn_

Cuts at:   1232    4769    4979    1232 
   Size:       3537     210    2999

  Fragments arranged by size: 

                3537    2999     210
 
SbfI CC_TGCA'GG

Cuts at:   3793    3793 
   Size:       6746
 
ScaI AGT'ACT

Cuts at:   2142    6263    2142 
   Size:       4121    2625
 
SexAI A'CCwGG_T

Cuts at:   2281    2281 
   Size:       6746
 
SfcI C'TryA_G

Cuts at:     33     552     734     935    1356    1396    1432    1978    2304 
   Size:        519     182     201     421      40      36     546     326

Cuts at:   2304    2332    2410    2569    2635    2734    3443    3789    4474 
   Size:         28      78     159      66      99     709     346     685

Cuts at:   4474    5467    5658      33 
   Size:        993     191    1121

  Fragments arranged by size: 

                1121     993     709     685     546     519     421     346
                 326     201     191     182     159      99      78      66
                  40      36      28
 
SfoI GGC'GCC

Cuts at:   1513    3699    4178    4426    1513 
   Size:       2186     479     248    3833

  Fragments arranged by size: 

                3833    2186     479     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6746
 
SmaI CCC'GGG

Cuts at:   3768    3768 
   Size:       6746
 
SmlI C'TyrA_G

Cuts at:   1768    2929    3460    3472    3901    4816    5308    5570    1768 
   Size:       1161     531      12     429     915     492     262    2944

  Fragments arranged by size: 

                2944    1161     915     531     492     429     262      12
            
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6746
 
SphI G_CATG'C

Cuts at:   1342    3799    4299    1342 
   Size:       2457     500    3789

  Fragments arranged by size: 

                3789    2457     500
 
StuI AGG'CCT

Cuts at:    676     982    1093    2653    2719    3084     676 
   Size:        306     111    1560      66     365    4338

  Fragments arranged by size: 

                4338    1560     365     306     111      66
 
StyI C'CwwG_G

Cuts at:   1803    2099    2168    3108    3337    3880    4234    1803 
   Size:        296      69     940     229     543     354    4315

  Fragments arranged by size: 

                4315     940     543     354     296     229      69
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1977    2464    2874    4329    4462    4921    5130    1977 
   Size:        487     410    1455     133     459     209    3593

  Fragments arranged by size: 

                3593    1455     487     459     410     209     133
 
TatI w'GTAC_w

Cuts at:    566     629    2140    2604    6261     566 
   Size:         63    1511     464    3657    1051

  Fragments arranged by size: 

                3657    1511    1051     464      63
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    516     548    1711    1743    1782    1814    6263    6295     516 
   Size:         32    1163      32      39      32    4449      32     967

  Fragments arranged by size: 

                4449    1163     967      39      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:    246    4543     246 
   Size:       4297    2449
 
XbaI T'CTAG_A

Cuts at:   3777    3777 
   Size:       6746
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2106    4241    2106 
   Size:       2135    4611
 
XmaI C'CCGG_G

Cuts at:   3766    3766 
   Size:       6746
 
XmnI GAAnn'nnTTC

Cuts at:    478    1777     478 
   Size:       1299    5447
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NspI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII    AflII     AhdI     BaeI    BfrBI     BmtI    BsiWI     BsmI
    BsmBI   BstEII     ClaI    CspCI    EcoRV     FseI    FspAI     HpaI
     MfeI     NheI     NotI     NruI     NsiI     PacI     PasI    PshAI
     PsiI    PspXI     PsrI    SacII     SfiI    SgrAI    SnaBI     SrfI
     SspI     SwaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com