Restriction map for pF1KA0536
 (Linear) (Six-base) MAPSORT of: KIAA0536.seq  Check: 3347  from: 1  to: 3021



KIAA0536.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:20  ..

AccI GT'mk_AC

Cuts at:      0    1266    1473    3021 
   Size:       1266     207    1548

  Fragments arranged by size: 

                1548    1266     207
 
AclI AA'CG_TT

Cuts at:      0    2055    3021 
   Size:       2055     966
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     452    1547    1688    2244    2713    2918    3021 
   Size:        452    1095     141     556     469     205     103

  Fragments arranged by size: 

                1095     556     469     452     205     141     103
 
AflIII A'CryG_T

Cuts at:      0    1679    2517    3021 
   Size:       1679     838     504

  Fragments arranged by size: 

                1679     838     504
 
AhdI GACnn_n'nnGTC

Cuts at:      0     915    3021 
   Size:        915    2106
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1338    1370    3021 
   Size:       1338      32    1651

  Fragments arranged by size: 

                1651    1338      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1906    2949    3021 
   Size:       1906    1043      72

  Fragments arranged by size: 

                1906    1043      72
 
ApoI r'AATT_y

Cuts at:      0     446    1507    2239    2657    3021 
   Size:        446    1061     732     418     364

  Fragments arranged by size: 

                1061     732     446     418     364
 
AseI AT'TA_AT

Cuts at:      0    2846    3021 
   Size:       2846     175
 
AvaI C'yCGr_G

Cuts at:      0    1247    3021 
   Size:       1247    1774
 
BanI G'GyrC_C

Cuts at:      0    1884    2882    3021 
   Size:       1884     998     139

  Fragments arranged by size: 

                1884     998     139
 
BanII G_rGCy'C

Cuts at:      0     404    2171    3021 
   Size:        404    1767     850

  Fragments arranged by size: 

                1767     850     404
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     104     194     326    1038    1580    1936    2350    2906 
   Size:        104      90     132     712     542     356     414     556

Cuts at:   2906    3021 
   Size:        115

  Fragments arranged by size: 

                 712     556     542     414     356     132     115     104
                  90
 
BclI T'GATC_A

Cuts at:      0    2769    3021 
   Size:       2769     252
 
BglII A'GATC_T

Cuts at:      0     487     766    1123    1330    1396    3021 
   Size:        487     279     357     207      66    1625

  Fragments arranged by size: 

                1625     487     357     279     207      66
 
BmgBI CAC'GTC

Cuts at:      0    1270    3021 
   Size:       1270    1751
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      12     877    2087    3021 
   Size:         12     865    1210     934

  Fragments arranged by size: 

                1210     934     865      12
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     444    3021 
   Size:        444    2577
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     263    1724    1774    3021 
   Size:        263    1461      50    1247

  Fragments arranged by size: 

                1461    1247     263      50
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     680     728    3021 
   Size:        680      48    2293

  Fragments arranged by size: 

                2293     680      48
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       7    1106    1313    1355    1382    1457    3021 
   Size:          7    1099     207      42      27      75    1564

  Fragments arranged by size: 

                1564    1099     207      75      42      27       7
 
BsaAI yAC'GTr

Cuts at:      0    1078    3021 
   Size:       1078    1943
 
BsaBI GATnn'nnATC

Cuts at:      0    1734    2225    2774    3021 
   Size:       1734     491     549     247

  Fragments arranged by size: 

                1734     549     491     247
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       4      34    1143    1173    1338    1368    3021 
   Size:          4      30    1109      30     165      30    1653

  Fragments arranged by size: 

                1653    1109     165      30      30      30       4
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1230    1391    1440    3021 
   Size:       1230     161      49    1581

  Fragments arranged by size: 

                1581    1230     161      49
 
BseYI C'CCAG_C

Cuts at:      0    1024    2961    3021 
   Size:       1024    1937      60

  Fragments arranged by size: 

                1937    1024      60
 
BsiEI CG_ry'CG

Cuts at:      0     861    3021 
   Size:        861    2160
 
BsiHKAI G_wGCw'C

Cuts at:      0    1941    2171    2571    2842    3021 
   Size:       1941     230     400     271     179

  Fragments arranged by size: 

                1941     400     271     230     179
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1212    3021 
   Size:       1212    1809
 
Bsp1286I G_dGCh'C

Cuts at:      0     404    1941    2171    2571    2842    3021 
   Size:        404    1537     230     400     271     179

  Fragments arranged by size: 

                1537     404     400     271     230     179
 
BspHI T'CATG_A

Cuts at:      0     450    3021 
   Size:        450    2571
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1088    1364    3021 
   Size:       1088     276    1657

  Fragments arranged by size: 

                1657    1088     276
 
BsrDI GCAATG_nn'

Cuts at:      0    2708    3021 
   Size:       2708     313
 
BsrGI T'GTAC_A

Cuts at:      0    2789    3021 
   Size:       2789     232
 
BstBI TT'CG_AA

Cuts at:      0    2739    3021 
   Size:       2739     282
 
BstEII G'GTnAC_C

Cuts at:      0    1019    3021 
   Size:       1019    2002
 
BstYI r'GATC_y

Cuts at:      0     487     559     766     877    1123    1330    1396    2377 
   Size:        487      72     207     111     246     207      66     981

Cuts at:   2377    2706    3021 
   Size:        329     315

  Fragments arranged by size: 

                 981     487     329     315     246     207     207     111
                  72      66
 
BtgI C'CryG_G

Cuts at:      0       8    3021 
   Size:          8    3013
 
DraI TTT'AAA

Cuts at:      0    1512    2189    2489    3021 
   Size:       1512     677     300     532

  Fragments arranged by size: 

                1512     677     532     300
 
DraIII CAC_nnn'GTG

Cuts at:      0    1465    3021 
   Size:       1465    1556
 
EaeI y'GGCC_r

Cuts at:      0       2    3021 
   Size:          2    3019
 
EarI CTCTTCn'nnn_

Cuts at:      0     431    1164    2263    3021 
   Size:        431     733    1099     758

  Fragments arranged by size: 

                1099     758     733     431
 
EcoICRI GAG'CTC

Cuts at:      0    2169    3021 
   Size:       2169     852
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     263     452    1547    1688    1724    1774    2244    2713 
   Size:        263     189    1095     141      36      50     470     469

Cuts at:   2713    2918    3021 
   Size:        205     103

  Fragments arranged by size: 

                1095     470     469     263     205     189     141     103
                  50      36
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2859    2988    3021 
   Size:       2859     129      33

  Fragments arranged by size: 

                2859     129      33
 
EcoRV GAT'ATC

Cuts at:      0    1257    2445    3021 
   Size:       1257    1188     576

  Fragments arranged by size: 

                1257    1188     576
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1537    3021 
   Size:       1537    1484
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       4      36     259     291     901     922     933     954 
   Size:          4      32     223      32     610      21      11      21

Cuts at:    954     985    1017    1108    1140    1143    1175    1241    1273 
   Size:         31      32      91      32       3      32      66      32

Cuts at:   1273    1534    1566    2414    2446    2491    2523    2602    2634 
   Size:        261      32     848      32      45      32      79      32

Cuts at:   2634    3021 
   Size:        387

  Fragments arranged by size: 

                 848     610     387     261     223      91      79      66
                  45      32      32      32      32      32      32      32
                  32      32      32      31      21      21      11       4
                   3
 
HindIII A'AGCT_T

Cuts at:      0    2491    2695    3021 
   Size:       2491     204     326

  Fragments arranged by size: 

                2491     326     204
 
MfeI C'AATT_G

Cuts at:      0    1640    3021 
   Size:       1640    1381
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1336    1402    2875    2918    2936    3021 
   Size:       1336      66    1473      43      18      85

  Fragments arranged by size: 

                1473    1336      85      66      43      18
 
MslI CAynn'nnrTG

Cuts at:      0    1684    3021 
   Size:       1684    1337
 
MspA1I CmG'CkG

Cuts at:      0      10    1954    3021 
   Size:         10    1944    1067

  Fragments arranged by size: 

                1944    1067      10
 
NdeI CA'TA_TG

Cuts at:      0    2687    3021 
   Size:       2687     334
 
NruI TCG'CGA

Cuts at:      0     857    3021 
   Size:        857    2164
 
NspI r_CATG'y

Cuts at:      0    1683    2440    2521    3021 
   Size:       1683     757      81     500

  Fragments arranged by size: 

                1683     757     500      81
 
PciI A'CATG_T

Cuts at:      0    1679    2517    3021 
   Size:       1679     838     504

  Fragments arranged by size: 

                1679     838     504
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2139    3021 
   Size:       2139     882
 
PfoI T'CCnGG_A

Cuts at:      0    3008    3021 
   Size:       3008      13
 
PmlI CAC'GTG

Cuts at:      0    1078    3021 
   Size:       1078    1943
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1230    1262    1338    1370    3021 
   Size:       1230      32      76      32    1651

  Fragments arranged by size: 

                1651    1230      76      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    2859    3021 
   Size:       2859     162
 
PshAI GACnn'nnGTC

Cuts at:      0     582    1418    3021 
   Size:        582     836    1603

  Fragments arranged by size: 

                1603     836     582
 
PsiI TTA'TAA

Cuts at:      0     838    3021 
   Size:        838    2183
 
PvuI CG_AT'CG

Cuts at:      0     861    3021 
   Size:        861    2160
 
SacI G_AGCT'C

Cuts at:      0    2171    3021 
   Size:       2171     850
 
SacII CC_GC'GG

Cuts at:      0      11    3021 
   Size:         11    3010
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1164    3021 
   Size:       1164    1857
 
ScaI AGT'ACT

Cuts at:      0     483    3021 
   Size:        483    2538
 
SexAI A'CCwGG_T

Cuts at:      0    1035    3021 
   Size:       1035    1986
 
SfcI C'TryA_G

Cuts at:      0    2382    2621    2992    3021 
   Size:       2382     239     371      29

  Fragments arranged by size: 

                2382     371     239      29
 
SmlI C'TyrA_G

Cuts at:      0     695     743    1247    3021 
   Size:        695      48     504    1774

  Fragments arranged by size: 

                1774     695     504      48
 
StuI AGG'CCT

Cuts at:      0    1813    3021 
   Size:       1813    1208
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1038    2020    2870    3021 
   Size:       1038     982     850     151

  Fragments arranged by size: 

                1038     982     850     151
 
TatI w'GTAC_w

Cuts at:      0     481     496    2789    2916    3021 
   Size:        481      15    2293     127     105

  Fragments arranged by size: 

                2293     481     127     105      15
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2832    2864    3021 
   Size:       2832      32     157

  Fragments arranged by size: 

                2832     157      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      18    1037    1155    3021 
   Size:         18    1019     118    1866

  Fragments arranged by size: 

                1866    1019     118      18
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2136    3021 
   Size:       2136     885
 
XhoI C'TCGA_G

Cuts at:      0    1247    3021 
   Size:       1247    1774
 
XmnI GAAnn'nnTTC

Cuts at:      0    1521    3021 
   Size:       1521    1500
 

 Enzymes that do cut:

     AccI     AclI     AcuI   AflIII     AhdI     AloI    AlwNI     ApoI
     AseI     AvaI     BanI    BanII     BbsI     BclI    BglII    BmgBI
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI
    BsrDI    BsrGI    BstBI   BstEII    BstYI     BtgI     DraI   DraIII
     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRV     FalI    Hin4I
  HindIII     MfeI     MmeI     MslI   MspA1I     NdeI     NruI     NspI
     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI    PshAI     PsiI
     PvuI     SacI    SacII     SapI     ScaI    SexAI     SfcI     SmlI
     StuI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII     AgeI     AleI     AlfI
     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI    BamHI     BbeI
    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I     BmtI
   Bpu10I    BsaHI    BsaWI     BsgI    BsiWI     BsmI    BspEI    BsrBI
    BsrFI   BssHII    BssSI   BstAPI    BstXI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DrdI     EagI     EciI    EcoNI    EcoRI
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MluI     MscI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NsiI     PacI     PasI     PmeI   PspOMI    PspXI     PsrI     PstI
    PvuII    RsrII     SalI    SanDI     SbfI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StyI
     SwaI     XbaI     XmaI     ZraI
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