Restriction map for pF1KA0366
##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AclI        AgeI        AhdI        AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        AspEI       
AspI        AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BcnI        BglI        BlnI        BsiEI       BsiWI       
BspDI       BssHII      BssSI       Bst1107I    BstBI       
BstZ17I     Bsu36I      ClaI        CpoI        DraIII      
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoRI       EcoRV       
FseI        HaeII       HpaI        KasI        KspI        
MluI        MluNI       MscI        NarI        NciI        
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SphI        Sse8387I    SwaI        Tth111I     XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII        3584  GACGTC               GACGT^C
Acc65I       2180  GGTACC               G^GTACC
AccI          598  GTCTAC               GT^[AC][GT]AC
AccIII        407  TCCGGA               T^CCGGA
AflII        2185  CTTAAG               C^TTAAG
Asp700I      2377  GAAAGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2180  GGTACC               G^GTACC
AvaI         2863  CCCGAG               C^[CT]CG[GA]G
AviII         917  TGCGCA               TGC^GCA
BclI         1383  TGATCA               T^GATCA
BfrI         2185  CTTAAG               C^TTAAG
BlpI         3400  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     3400  GCTCAGC              GC^T[ACGT]AGC
BsaAI         396  TACGTA               [CT]AC^GT[GA]
BsaBI         268  GATTTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         407  TCCGGA               [AT]^CCGG[AT]
BseAI         407  TCCGGA               T^CCGGA
BsmBI         735  GAGACG               CGTCTC(1/5)
BsoBI        2863  CCCGAG               C^[CT]CG[GA]G
Bsp1407I     2710  TGTACA               T^GTACA
BspEI         407  TCCGGA               T^CCGGA
BsrGI        2710  TGTACA               T^GTACA
CelII        3400  GCTCAGC              GC^T[ACGT]AGC
DraI         2292  TTTAAA               TTT^AAA
DrdI          796  GACTCTGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          813  CCATGG               C^C[GA][CT]GG
EcoT22I      3338  ATGCAT               ATGCA^T
FbaI         1383  TGATCA               T^GATCA
FspI          917  TGCGCA               TGC^GCA
HincII       3029  GTCAAC               GT[CT]^[GA]AC
HindII       3029  GTCAAC               GT[CT]^[GA]AC
KpnI         2180  GGTACC               GGTAC^C
MamI          268  GATTTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1656  CAATTG               C^AATTG
MroI          407  TCCGGA               T^CCGGA
MunI         1656  CAATTG               C^AATTG
NaeI         2873  GCCGGC               GCC^GGC
NcoI          813  CCATGG               C^CATGG
NgoMIV       2873  GCCGGC               G^CCGGC
NsiI         3338  ATGCAT               ATGCA^T
PshAI         135  GACTCCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI         2553  GAAGAGC              GCTCTTC(1/4)
SexAI        3117  ACCAGGT              A^CC[AT]GGT
SnaBI         396  TACGTA               TAC^GTA
SpeI          300  ACTAGT               A^CTAGT
SspBI        2710  TGTACA               T^GTACA
SspI         2698  AATATT               AAT^ATT
StuI          675  AGGCCT               AGG^CCT
TaqI          773  TCGA                 T^CGA
TthHB8I       773  TCGA                 T^CGA
XmnI         2377  GAAAGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI          869  AAATTT               [GA]^AATT[CT]
             1675  AAATTT              
Alw44I       1476  GTGCAC               G^TGCAC
             2833  GTGCAC              
ApaLI        1476  GTGCAC               G^TGCAC
             2833  GTGCAC              
ApoI          869  AAATTT               [GA]^AATT[CT]
             1675  AAATTT              
BsaI         3283  GAGACC               GGTCTC(1/5)
             2561  GGTCTC              
BsgI         2748  CTGCAC               GTGCAG(16/14)
             2900  CTGCAC              
BsrDI        1615  CATTGC               GCAATG(2/0)
             1440  GCAATG              
BsrFI        1117  ACCGGC               [GA]^CCGG[CT]
             2873  GCCGGC              
BstAPI       2840  GCAAATACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3340  GCATATGCTGC         
BstEII       2854  GGTGACC              G^GT[ACGT]ACC
             3432  GGTCACC             
BstPI        2854  GGTGACC              G^GT[ACGT]ACC
             3432  GGTCACC             
BstXI         977  CCAGAAGCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2991  CCACTGTGATGG        
CfoI          918  GCGC                 GCG^C
             3156  GCGC                
Cfr10I       1117  ACCGGC               [GA]^CCGG[CT]
             2873  GCCGGC              
EcoO65I      2854  GGTGACC              G^GT[ACGT]ACC
             3432  GGTCACC             
FauI         2447  CCCGC                CCCGC(4/6)
              751  GCGGG               
HhaI          918  GCGC                 GCG^C
             3156  GCGC                
HinP1I        918  GCGC                 G^CGC
             3156  GCGC                
HindIII       981  AAGCTT               A^AGCTT
             2173  AAGCTT              
PflMI        1203  CCATGTGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1567  CCACTTGATGG         
PstI         2976  CTGCAG               CTGCA^G
             3147  CTGCAG              
SmlI         2185  CTTAAG               C^T[CT][GA]AG
             2432  CTCAAG              
Van91I       1203  CCATGTGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1567  CCACTTGATGG         
XbaI          153  TCTAGA               T^CTAGA
             3186  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       AspHI       AvaII       
BanI        BbsI        BbvI        BfaI        BglII       
BmyI        BpmI        BpuAI       BsaHI       BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    BspHI       BspLU11I    
BspMI       BsrBI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       EarI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HgaI        Hin1I       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NdeI        NdeII       NlaIII      
NlaIV       NspI        PleI        PpuMI       PvuII       
RcaI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XhoII       
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