Restriction map for pF1KA0356
##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
Aor51HI     AscI        AseI        AsnI        Asp718I     
AspI        AvrII       BalI        BcgI        BciVI       
BclI        BfrI        BlnI        BsaBI       BseAI       
BsiEI       BsiWI       BsmI        BspDI       BspEI       
BspLU11I    BssSI       Bst1107I    BstAPI      BstZ17I     
ClaI        CpoI        DraI        EagI        EclXI       
Eco47III    Eco52I      EcoRV       EcoT22I     FbaI        
FseI        HindIII     HpaI        KpnI        MamI        
MfeI        MluNI       MroI        MscI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PmeI        PshBI       
Psp1406I    PvuI        RsrII       SalI        SfiI        
SnaBI       SphI        SspI        SwaI        Tth111I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI         2962  GTCTAC               GT^[AC][GT]AC
AgeI          908  ACCGGT               A^CCGGT
AhdI         2017  GACTGGTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2979  GTGCAC               G^TGCAC
ApaLI        2979  GTGCAC               G^TGCAC
AspEI        2017  GACTGGTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        2491  TGCGCA               TGC^GCA
BamHI          70  GGATCC               G^GATCC
BbrPI        1114  CACGTG               CAC^GTG
BglII        3005  AGATCT               A^GATCT
BlpI         2826  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2826  GCTCAGC              GC^T[ACGT]AGC
Bsp1407I     2075  TGTACA               T^GTACA
BsrGI        2075  TGTACA               T^GTACA
BssHII       1815  GCGCGC               G^CGCGC
BstBI         526  TTCGAA               TT^CGAA
BstEII       2358  GGTGACC              G^GT[ACGT]ACC
BstPI        2358  GGTGACC              G^GT[ACGT]ACC
CelII        2826  GCTCAGC              GC^T[ACGT]AGC
Eam1105I     2017  GACTGGTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1657  CTCTTC               CTCTTC(1/4)
EcoO65I      2358  GGTGACC              G^GT[ACGT]ACC
EcoRI         934  GAATTC               G^AATTC
FspI         2491  TGCGCA               TGC^GCA
HincII        331  GTCAAC               GT[CT]^[GA]AC
HindII        331  GTCAAC               GT[CT]^[GA]AC
Ksp632I      1657  CTCTTC               CTCTTC(1/4)
KspI         1991  CCGCGG               CCGC^GG
MluI         2714  ACGCGT               A^CGCGT
MseI          553  TTAA                 T^TAA
NaeI         3134  GCCGGC               GCC^GGC
NgoMIV       3134  GCCGGC               G^CCGGC
NspV          526  TTCGAA               TT^CGAA
PinAI         908  ACCGGT               A^CCGGT
PmaCI        1114  CACGTG               CAC^GTG
PmlI         1114  CACGTG               CAC^GTG
PshAI         324  GACGTTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII        1991  CCGCGG               CCGC^GG
SapI         1656  GCTCTTC              GCTCTTC(1/4)
SbfI          501  CCTGCAGG             CCTGCA^GG
SexAI        2441  ACCTGGT              A^CC[AT]GGT
SfuI          526  TTCGAA               TT^CGAA
SgrAI        3133  CGCCGGCG             C[GA]^CCGG[CT]G
SpeI          121  ACTAGT               A^CTAGT
Sse8387I      501  CCTGCAGG             CCTGCA^GG
SspBI        2075  TGTACA               T^GTACA
Tru9I         553  TTAA                 T^TAA
XcmI         1083  CCAGGCAGCCTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AflIII         98  ACGTGT               A^C[GA][CT]GT
             2714  ACGCGT              
AocI         1178  CCTCAGG              CC^T[ACGT]AGG
             1930  CCTGAGG             
ApaI         2223  GGGCCC               GGGCC^C
             2681  GGGCCC              
BfaI          122  CTAG                 C^TAG
             1196  CTAG                
BsaAI          97  TACGTG               [CT]AC^GT[GA]
             1114  CACGTG              
BsaWI         908  ACCGGT               [AT]^CCGG[AT]
             1565  ACCGGA              
BsmBI        2717  CGTCTC               CGTCTC(1/5)
             2760  GAGACG              
BspHI        1260  TCATGA               T^CATGA
             1604  TCATGA              
BsrBI        1624  GAGCGG               CCGCTC(-3/-3)
             2737  GAGCGG              
BsrDI         847  CATTGC               GCAATG(2/0)
             1636  GCAATG              
Bsu36I       1178  CCTCAGG              CC^T[ACGT]AGG
             1930  CCTGAGG             
DraIII        144  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2176  CACGGCGTG           
Eco81I       1178  CCTCAGG              CC^T[ACGT]AGG
             1930  CCTGAGG             
MaeI          122  CTAG                 C^TAG
             1196  CTAG                
NspI         2730  GCATGT               [GA]CATG^[CT]
             2958  GCATGT              
PflMI        2175  CCACGGCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3096  CCAGGCTGTGG         
PpuMI        2086  AGGACCT              [GA]G^G[AT]CC[CT]
             2624  GGGACCT             
PvuII         781  CAGCTG               CAG^CTG
             2770  CAGCTG              
RcaI         1260  TCATGA               T^CATGA
             1604  TCATGA              
ScaI          440  AGTACT               AGT^ACT
             1198  AGTACT              
SmaI         1352  CCCGGG               CCC^GGG
             2414  CCCGGG              
TaqI          527  TCGA                 T^CGA
              695  TCGA                
TthHB8I       527  TCGA                 T^CGA
              695  TCGA                
Van91I       2175  CCACGGCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3096  CCAGGCTGTGG         
XmaCI        1352  CCCGGG               C^CCGGG
             2414  CCCGGG              
XmaI         1352  CCCGGG               C^CCGGG
             2414  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApoI        Asp700I     
AspHI       AvaI        AvaII       BanI        BanII       
BbeI        BbsI        BbvCI       BbvI        BcnI        
BglI        BmyI        BpmI        BpuAI       BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DrdI        DsaI        EaeI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NcoI        NdeII       
NlaIII      NlaIV       PleI        PstI        RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmlI        StuI        StyI        
TfiI        TseI        Tsp45I      Tsp509I     TspRI       
XhoII       XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com