Restriction map for pF1KA0197
 (Linear) (Six-base) MAPSORT of: KIAA0197.seq  Check: 4825  from: 1  to: 3945



KIAA0197.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:20  ..

AccI GT'mk_AC

Cuts at:      0    2761    3945 
   Size:       2761    1184
 
Acc65I G'GTAC_C

Cuts at:      0    1583    3945 
   Size:       1583    2362
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     722    1396    1486    2720    2879    3945 
   Size:        722     674      90    1234     159    1066

  Fragments arranged by size: 

                1234    1066     722     674     159      90
 
AflII C'TTAA_G

Cuts at:      0     616    3945 
   Size:        616    3329
 
AflIII A'CryG_T

Cuts at:      0    2899    3945 
   Size:       2899    1046
 
AleI CACnn'nnGTG

Cuts at:      0    3818    3945 
   Size:       3818     127
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2068    3667    3945 
   Size:       2068    1599     278

  Fragments arranged by size: 

                2068    1599     278
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     596     628    3945 
   Size:        596      32    3317

  Fragments arranged by size: 

                3317     596      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     517    1212    1747    1821    2112    2314    2698    3334 
   Size:        517     695     535      74     291     202     384     636

Cuts at:   3334    3514    3945 
   Size:        180     431

  Fragments arranged by size: 

                 695     636     535     517     431     384     291     202
                 180      74
 
ApaI G_GGCC'C

Cuts at:      0    1119    3945 
   Size:       1119    2826
 
ApaLI G'TGCA_C

Cuts at:      0     376    3945 
   Size:        376    3569
 
ApoI r'AATT_y

Cuts at:      0      76     286    1350    1435    1458    1471    2716    2963 
   Size:         76     210    1064      85      23      13    1245     247

Cuts at:   2963    3945 
   Size:        982

  Fragments arranged by size: 

                1245    1064     982     247     210      85      76      23
                  13
 
AvaI C'yCGr_G

Cuts at:      0    1694    3945 
   Size:       1694    2251
 
BanI G'GyrC_C

Cuts at:      0     118    1583    2427    3945 
   Size:        118    1465     844    1518

  Fragments arranged by size: 

                1518    1465     844     118
 
BanII G_rGCy'C

Cuts at:      0      15    1119    3945 
   Size:         15    1104    2826

  Fragments arranged by size: 

                2826    1104      15
 
BbeI G_GCGC'C

Cuts at:      0     122    3945 
   Size:        122    3823
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     217    1492    3945 
   Size:        217    1275    2453

  Fragments arranged by size: 

                2453    1275     217
 
BclI T'GATC_A

Cuts at:      0    1242    1832    3945 
   Size:       1242     590    2113

  Fragments arranged by size: 

                2113    1242     590
 
BfrBI ATG'CAT

Cuts at:      0     975    3644    3945 
   Size:        975    2669     301

  Fragments arranged by size: 

                2669     975     301
 
BglI GCCn_nnn'nGGC

Cuts at:      0    3864    3945 
   Size:       3864      81
 
BglII A'GATC_T

Cuts at:      0    2091    3438    3945 
   Size:       2091    1347     507

  Fragments arranged by size: 

                2091    1347     507
 
BlpI GC'TnA_GC

Cuts at:      0      37      47     556    1976    2168    3485    3945 
   Size:         37      10     509    1420     192    1317     460

  Fragments arranged by size: 

                1420    1317     509     460     192      37      10
 
Bme1580I G_kGCm'C

Cuts at:      0     380    1119    3945 
   Size:        380     739    2826

  Fragments arranged by size: 

                2826     739     380
 
BmtI G_CTAG'C

Cuts at:      0    3700    3945 
   Size:       3700     245
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     239     332     889    1386    2290    3377    3463    3802 
   Size:        239      93     557     497     904    1087      86     339

Cuts at:   3802    3945 
   Size:        143

  Fragments arranged by size: 

                1087     904     557     497     339     239     143      93
                  86
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3268    3945 
   Size:       3268     677
 
BsaAI yAC'GTr

Cuts at:      0     159    3100    3945 
   Size:        159    2941     845

  Fragments arranged by size: 

                2941     845     159
 
BsaBI GATnn'nnATC

Cuts at:      0    1231    3945 
   Size:       1231    2714
 
BsaHI Gr'CG_yC

Cuts at:      0     119    3945 
   Size:        119    3826
 
BsaWI w'CCGG_w

Cuts at:      0    1797    3945 
   Size:       1797    2148
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     562     592     829     859    2120    2150    2743    2773 
   Size:        562      30     237      30    1261      30     593      30

Cuts at:   2773    3436    3466    3945 
   Size:        663      30     479

  Fragments arranged by size: 

                1261     663     593     562     479     237      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     256    1462    3677    3945 
   Size:        256    1206    2215     268

  Fragments arranged by size: 

                2215    1206     268     256
 
BseYI C'CCAG_C

Cuts at:      0    2006    2631    3945 
   Size:       2006     625    1314

  Fragments arranged by size: 

                2006    1314     625
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2064    3343    3359    3945 
   Size:       2064    1279      16     586

  Fragments arranged by size: 

                2064    1279     586      16
 
BsiHKAI G_wGCw'C

Cuts at:      0     380    1021    3945 
   Size:        380     641    2924

  Fragments arranged by size: 

                2924     641     380
 
BsmI GAATG_Cn'

Cuts at:      0    3215    3394    3945 
   Size:       3215     179     551

  Fragments arranged by size: 

                3215     551     179
 
Bsp1286I G_dGCh'C

Cuts at:      0      15     380    1021    1119    3945 
   Size:         15     365     641      98    2826

  Fragments arranged by size: 

                2826     641     365      98      15
 
BspEI T'CCGG_A

Cuts at:      0    1797    3945 
   Size:       1797    2148
 
BspHI T'CATG_A

Cuts at:      0    3378    3945 
   Size:       3378     567
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1171    3945 
   Size:       1171    2774
 
BsrGI T'GTAC_A

Cuts at:      0    2559    3945 
   Size:       2559    1386
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     690    1212    3945 
   Size:        690     522    2733

  Fragments arranged by size: 

                2733     690     522
 
BstBI TT'CG_AA

Cuts at:      0    1455    1992    3231    3945 
   Size:       1455     537    1239     714

  Fragments arranged by size: 

                1455    1239     714     537
 
BstEII G'GTnAC_C

Cuts at:      0     724    3945 
   Size:        724    3221
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1909    2014    3780    3945 
   Size:       1909     105    1766     165

  Fragments arranged by size: 

                1909    1766     165     105
 
BstYI r'GATC_y

Cuts at:      0     483    1356    2091    3039    3438    3945 
   Size:        483     873     735     948     399     507

  Fragments arranged by size: 

                 948     873     735     507     483     399
 
BtgI C'CryG_G

Cuts at:      0     110     947    1103    3945 
   Size:        110     837     156    2842

  Fragments arranged by size: 

                2842     837     156     110
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3870    3945 
   Size:       3870      75
 
BtsI GCAGTG_nn'

Cuts at:      0     306     567    3347    3572    3945 
   Size:        306     261    2780     225     373

  Fragments arranged by size: 

                2780     373     306     261     225
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      92     127    3945 
   Size:         92      35    3818

  Fragments arranged by size: 

                3818      92      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3299    3945 
   Size:       3299     646
 
EarI CTCTTCn'nnn_

Cuts at:      0    1217    1730    2416    3945 
   Size:       1217     513     686    1529

  Fragments arranged by size: 

                1529    1217     686     513
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3460    3945 
   Size:       3460     485
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     239     332     722     889    1386    1396    1486    2290 
   Size:        239      93     390     167     497      10      90     804

Cuts at:   2290    2720    2879    3377    3463    3802    3945 
   Size:        430     159     498      86     339     143

  Fragments arranged by size: 

                 804     498     497     430     390     339     239     167
                 159     143      93      90      86      10
 
EcoO109I rG'GnC_Cy

Cuts at:      0     573    1116    3945 
   Size:        573     543    2829

  Fragments arranged by size: 

                2829     573     543
 
EcoRI G'AATT_C

Cuts at:      0      76    1435    1471    2716    3945 
   Size:         76    1359      36    1245    1229

  Fragments arranged by size: 

                1359    1245    1229      76      36
 
EcoRV GAT'ATC

Cuts at:      0    1110    1689    3945 
   Size:       1110     579    2256

  Fragments arranged by size: 

                2256    1110     579
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3624    3945 
   Size:       3624     321
 
HaeII r_GCGC'y

Cuts at:      0     122    3945 
   Size:        122    3823
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     324     356    2743    2775    3945 
   Size:        324      32    2387      32    1170

  Fragments arranged by size: 

                2387    1170     324      32      32
 
HincII GTy'rAC

Cuts at:      0     362     432    3945 
   Size:        362      70    3513

  Fragments arranged by size: 

                3513     362      70
 
HindIII A'AGCT_T

Cuts at:      0    1304    3601    3945 
   Size:       1304    2297     344

  Fragments arranged by size: 

                2297    1304     344
 
HpaI GTT'AAC

Cuts at:      0     362    3945 
   Size:        362    3583
 
KasI G'GCGC_C

Cuts at:      0     118    3945 
   Size:        118    3827
 
KpnI G_GTAC'C

Cuts at:      0    1587    3945 
   Size:       1587    2358
 
MfeI C'AATT_G

Cuts at:      0    1907    2563    3282    3945 
   Size:       1907     656     719     663

  Fragments arranged by size: 

                1907     719     663     656
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2307    3916    3945 
   Size:       2307    1609      29

  Fragments arranged by size: 

                2307    1609      29
 
MslI CAynn'nnrTG

Cuts at:      0    1558    3193    3818    3945 
   Size:       1558    1635     625     127

  Fragments arranged by size: 

                1635    1558     625     127
 
MspA1I CmG'CkG

Cuts at:      0    2112    3397    3502    3945 
   Size:       2112    1285     105     443

  Fragments arranged by size: 

                2112    1285     443     105
 
NarI GG'CG_CC

Cuts at:      0     119    3945 
   Size:        119    3826
 
NcoI C'CATG_G

Cuts at:      0     947    3945 
   Size:        947    2998
 
NdeI CA'TA_TG

Cuts at:      0    1705    2946    3945 
   Size:       1705    1241     999

  Fragments arranged by size: 

                1705    1241     999
 
NheI G'CTAG_C

Cuts at:      0    3696    3945 
   Size:       3696     249
 
NsiI A_TGCA'T

Cuts at:      0     977    3646    3945 
   Size:        977    2669     299

  Fragments arranged by size: 

                2669     977     299
 
NspI r_CATG'y

Cuts at:      0     867     975     979    2903    3945 
   Size:        867     108       4    1924    1042

  Fragments arranged by size: 

                1924    1042     867     108       4
 
PciI A'CATG_T

Cuts at:      0    2899    3945 
   Size:       2899    1046
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3334    3945 
   Size:       3334     611
 
PfoI T'CCnGG_A

Cuts at:      0     403    1363    3425    3945 
   Size:        403     960    2062     520

  Fragments arranged by size: 

                2062     960     520     403
 
PmlI CAC'GTG

Cuts at:      0    3100    3945 
   Size:       3100     845
 
PsiI TTA'TAA

Cuts at:      0    1714    1765    1784    3871    3945 
   Size:       1714      51      19    2087      74

  Fragments arranged by size: 

                2087    1714      74      51      19
 
PspOMI G'GGCC_C

Cuts at:      0    1115    3945 
   Size:       1115    2830
 
PstI C_TGCA'G

Cuts at:      0      91    1812    2089    2777    3945 
   Size:         91    1721     277     688    1168

  Fragments arranged by size: 

                1721    1168     688     277      91
 
PvuII CAG'CTG

Cuts at:      0    2112    3397    3945 
   Size:       2112    1285     548

  Fragments arranged by size: 

                2112    1285     548
 
ScaI AGT'ACT

Cuts at:      0    2070    2297    3945 
   Size:       2070     227    1648

  Fragments arranged by size: 

                2070    1648     227
 
SexAI A'CCwGG_T

Cuts at:      0     639    3945 
   Size:        639    3306
 
SfcI C'TryA_G

Cuts at:      0      87     545    1035    1789    1808    2085    2773    3034 
   Size:         87     458     490     754      19     277     688     261

Cuts at:   3034    3746    3945 
   Size:        712     199

  Fragments arranged by size: 

                 754     712     688     490     458     277     261     199
                  87      19
 
SfoI GGC'GCC

Cuts at:      0     120    3945 
   Size:        120    3825
 
SmlI C'TyrA_G

Cuts at:      0     616    3247    3945 
   Size:        616    2631     698

  Fragments arranged by size: 

                2631     698     616
 
SphI G_CATG'C

Cuts at:      0     979    3945 
   Size:        979    2966
 
SspI AAT'ATT

Cuts at:      0     452    1989    3945 
   Size:        452    1537    1956

  Fragments arranged by size: 

                1956    1537     452
 
StyI C'CwwG_G

Cuts at:      0     947    3458    3945 
   Size:        947    2511     487

  Fragments arranged by size: 

                2511     947     487
 
TatI w'GTAC_w

Cuts at:      0    2068    2295    2559    3945 
   Size:       2068     227     264    1386

  Fragments arranged by size: 

                2068    1386     264     227
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     829     861    2120    2152    3436    3468    3945 
   Size:        829      32    1259      32    1284      32     477

  Fragments arranged by size: 

                1284    1259     829     477      32      32      32
 
XbaI T'CTAG_A

Cuts at:      0    2529    3945 
   Size:       2529    1416
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    3311    3945 
   Size:       3311     634
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI    AflII   AflIII     AleI     AlfI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AvaI     BanI    BanII     BbeI
     BbsI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I     BmtI
     BpmI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrGI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI    CspCI     DrdI     EarI     EciI  Eco57MI EcoO109I    EcoRI
    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MmeI     MslI   MspA1I     NarI     NcoI     NdeI
     NheI     NsiI     NspI     PciI    PflMI     PfoI     PmlI     PsiI
   PspOMI     PstI    PvuII     ScaI    SexAI     SfcI     SfoI     SmlI
     SphI     SspI     StyI     TatI     TstI     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI     AgeI     AhdI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI    BmgBI
     BmrI     BplI   Bpu10I     BsaI    BsiEI    BsiWI    BsmBI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI  BstZ17I   Bsu36I     ClaI     DraI
   DraIII     EaeI     EagI  EcoICRI    EcoNI     FseI     FspI    FspAI
     MluI     MscI     NaeI   NgoMIV     NotI     NruI     PacI     PasI
     PmeI     PpiI    PpuMI    PshAI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     StuI     SwaI    TaqII
  Tth111I     XhoI     XmaI     XmnI     ZraI
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