Restriction map for pF1KA0051
 (Linear) (Six-base) MAPSORT of: KIAA0051.seq  Check: 7478  from: 1  to: 4971



KIAA0051.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2347    2630    4557    4971 
   Size:       2347     283    1927     414

  Fragments arranged by size: 

                2347    1927     414     283
 
AatII G_ACGT'C

Cuts at:      0     108    4971 
   Size:        108    4863
 
AccI GT'mk_AC

Cuts at:      0     227    4971 
   Size:        227    4744
 
Acc65I G'GTAC_C

Cuts at:      0    4481    4971 
   Size:       4481     490
 
AclI AA'CG_TT

Cuts at:      0    4167    4971 
   Size:       4167     804
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1531    2027    2335    3520    3571    3679    3808    4971 
   Size:       1531     496     308    1185      51     108     129    1163

  Fragments arranged by size: 

                1531    1185    1163     496     308     129     108      51
            
 
AfeI AGC'GCT

Cuts at:      0    2470    4971 
   Size:       2470    2501
 
AflII C'TTAA_G

Cuts at:      0    3741    4971 
   Size:       3741    1230
 
AflIII A'CryG_T

Cuts at:      0    3035    4971 
   Size:       3035    1936
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     601    4295    4971 
   Size:        601    3694     676

  Fragments arranged by size: 

                3694     676     601
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2264    2296    4971 
   Size:       2264      32    2675

  Fragments arranged by size: 

                2675    2264      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1050    1450    2442    2715    2922    4440    4863    4971 
   Size:       1050     400     992     273     207    1518     423     108

  Fragments arranged by size: 

                1518    1050     992     423     400     273     207     108
            
 
ApoI r'AATT_y

Cuts at:      0     692     884    1540    1814    2133    2145    2290    2307 
   Size:        692     192     656     274     319      12     145      17

Cuts at:   2307    2611    3781    3832    4816    4971 
   Size:        304    1170      51     984     155

  Fragments arranged by size: 

                1170     984     692     656     319     304     274     192
                 155     145      51      17      12
 
AseI AT'TA_AT

Cuts at:      0     671    1184    1634    1889    3752    4971 
   Size:        671     513     450     255    1863    1219

  Fragments arranged by size: 

                1863    1219     671     513     450     255
 
AvaI C'yCGr_G

Cuts at:      0    2162    2552    4971 
   Size:       2162     390    2419

  Fragments arranged by size: 

                2419    2162     390
 
AvrII C'CTAG_G

Cuts at:      0     171    4642    4971 
   Size:        171    4471     329

  Fragments arranged by size: 

                4471     329     171
 
BamHI G'GATC_C

Cuts at:      0    2586    4971 
   Size:       2586    2385
 
BanI G'GyrC_C

Cuts at:      0    3181    3999    4481    4971 
   Size:       3181     818     482     490

  Fragments arranged by size: 

                3181     818     490     482
 
BanII G_rGCy'C

Cuts at:      0     758     981    1349    3679    4971 
   Size:        758     223     368    2330    1292

  Fragments arranged by size: 

                2330    1292     758     368     223
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     562    1222    3388    4343    4971 
   Size:        562     660    2166     955     628

  Fragments arranged by size: 

                2166     955     660     628     562
 
BbvCI CC'TCA_GC

Cuts at:      0    2582    4971 
   Size:       2582    2389
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     691     717     725     751    2143    2177    2443    2477 
   Size:        691      26       8      26    1392      34     266      34

Cuts at:   2477    4971 
   Size:       2494

  Fragments arranged by size: 

                2494    1392     691     266      34      34      26      26
                   8
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     463    1274    2266    2356    4971 
   Size:        463     811     992      90    2615

  Fragments arranged by size: 

                2615     992     811     463      90
 
BclI T'GATC_A

Cuts at:      0    1385    2243    4442    4971 
   Size:       1385     858    2199     529

  Fragments arranged by size: 

                2199    1385     858     529
 
BglII A'GATC_T

Cuts at:      0     180    3209    4971 
   Size:        180    3029    1762

  Fragments arranged by size: 

                3029    1762     180
 
BlpI GC'TnA_GC

Cuts at:      0     794    4971 
   Size:        794    4177
 
Bme1580I G_kGCm'C

Cuts at:      0    1935    3186    4004    4971 
   Size:       1935    1251     818     967

  Fragments arranged by size: 

                1935    1251     967     818
 
BmgBI CAC'GTC

Cuts at:      0    4110    4279    4971 
   Size:       4110     169     692

  Fragments arranged by size: 

                4110     692     169
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     250    2073    3214    3762    4971 
   Size:        250    1823    1141     548    1209

  Fragments arranged by size: 

                1823    1209    1141     548     250
 
BmtI G_CTAG'C

Cuts at:      0    3367    4971 
   Size:       3367    1604
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223    1139    2119    2737    4136    4971 
   Size:        223     916     980     618    1399     835

  Fragments arranged by size: 

                1399     980     916     835     618     223
 
Bpu10I CC'TnA_GC

Cuts at:      0     214    1517    2582    3353    4971 
   Size:        214    1303    1065     771    1618

  Fragments arranged by size: 

                1618    1303    1065     771     214
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1723    2857    4971 
   Size:       1723    1134    2114

  Fragments arranged by size: 

                2114    1723    1134
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2095    2875    4198    4971 
   Size:       2095     780    1323     773

  Fragments arranged by size: 

                2095    1323     780     773
 
BsaBI GATnn'nnATC

Cuts at:      0    1283    4971 
   Size:       1283    3688
 
BsaHI Gr'CG_yC

Cuts at:      0     105    4971 
   Size:        105    4866
 
BsaWI w'CCGG_w

Cuts at:      0    3951    4187    4971 
   Size:       3951     236     784

  Fragments arranged by size: 

                3951     784     236
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1099    1129    1354    1384    2392    2422    4877    4907 
   Size:       1099      30     225      30    1008      30    2455      30

Cuts at:   4907    4971 
   Size:         64

  Fragments arranged by size: 

                2455    1099    1008     225      64      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     100     220     877    1120    2182    2668    3067    4506 
   Size:        100     120     657     243    1062     486     399    1439

Cuts at:   4506    4971 
   Size:        465

  Fragments arranged by size: 

                1439    1062     657     486     465     399     243     120
                 100
 
BseYI C'CCAG_C

Cuts at:      0      27    1145    1247    1727    4971 
   Size:         27    1118     102     480    3244

  Fragments arranged by size: 

                3244    1118     480     102      27
 
BsiEI CG_ry'CG

Cuts at:      0      43    4971 
   Size:         43    4928
 
BsiHKAI G_wGCw'C

Cuts at:      0    1349    3959    4971 
   Size:       1349    2610    1012

  Fragments arranged by size: 

                2610    1349    1012
 
BsmI GAATG_Cn'

Cuts at:      0     366     735    2342    3201    4131    4971 
   Size:        366     369    1607     859     930     840

  Fragments arranged by size: 

                1607     930     859     840     369     366
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      96    4269    4971 
   Size:         96    4173     702

  Fragments arranged by size: 

                4173     702      96
 
Bsp1286I G_dGCh'C

Cuts at:      0     758     981    1349    1935    3186    3679    3959    4004 
   Size:        758     223     368     586    1251     493     280      45

Cuts at:   4004    4971 
   Size:        967

  Fragments arranged by size: 

                1251     967     758     586     493     368     280     223
                  45
 
BspEI T'CCGG_A

Cuts at:      0    3951    4971 
   Size:       3951    1020
 
BspHI T'CATG_A

Cuts at:      0    3369    4895    4971 
   Size:       3369    1526      76

  Fragments arranged by size: 

                3369    1526      76
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2347    2411    2630    3068    4557    4606    4745    4871 
   Size:       2347      64     219     438    1489      49     139     126

Cuts at:   4871    4971 
   Size:        100

  Fragments arranged by size: 

                2347    1489     438     219     139     126     100      64
                  49
 
BsrDI GCAATG_nn'

Cuts at:      0    3485    3680    3944    4451    4971 
   Size:       3485     195     264     507     520

  Fragments arranged by size: 

                3485     520     507     264     195
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     652    1138    4971 
   Size:        652     486    3833

  Fragments arranged by size: 

                3833     652     486
 
BstBI TT'CG_AA

Cuts at:      0    4802    4971 
   Size:       4802     169
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     201    2955    3003    4821    4971 
   Size:        201    2754      48    1818     150

  Fragments arranged by size: 

                2754    1818     201     150      48
 
BstYI r'GATC_y

Cuts at:      0     180    2171    2586    3209    4373    4971 
   Size:        180    1991     415     623    1164     598

  Fragments arranged by size: 

                1991    1164     623     598     415     180
 
Bsu36I CC'TnA_GG

Cuts at:      0    1872    4971 
   Size:       1872    3099
 
BtgI C'CryG_G

Cuts at:      0     365    2268    3177    3186    4412    4971 
   Size:        365    1903     909       9    1226     559

  Fragments arranged by size: 

                1903    1226     909     559     365       9
 
BtsI GCAGTG_nn'

Cuts at:      0    1364    4971 
   Size:       1364    3607
 
ClaI AT'CG_AT

Cuts at:      0    3579    4971 
   Size:       3579    1392
 
DraI TTT'AAA

Cuts at:      0    4043    4388    4755    4971 
   Size:       4043     345     367     216

  Fragments arranged by size: 

                4043     367     345     216
 
DraIII CAC_nnn'GTG

Cuts at:      0    1930    4971 
   Size:       1930    3041
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1079    4080    4971 
   Size:       1079    3001     891

  Fragments arranged by size: 

                3001    1079     891
 
EaeI y'GGCC_r

Cuts at:      0      40    2228    2957    3189    4971 
   Size:         40    2188     729     232    1782

  Fragments arranged by size: 

                2188    1782     729     232      40
 
EagI C'GGCC_G

Cuts at:      0      40    4971 
   Size:         40    4931
 
EarI CTCTTCn'nnn_

Cuts at:      0     145     746    1063    1753    2675    2887    4358    4971 
   Size:        145     601     317     690     922     212    1471     613

  Fragments arranged by size: 

                1471     922     690     613     601     317     212     145
            
 
EcoICRI GAG'CTC

Cuts at:      0    1347    4971 
   Size:       1347    3624
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223    1139    1531    2027    2119    2335    2737    3520 
   Size:        223     916     392     496      92     216     402     783

Cuts at:   3520    3571    3679    3808    4136    4971 
   Size:         51     108     129     328     835

  Fragments arranged by size: 

                 916     835     783     496     402     392     328     223
                 216     129     108      92      51
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     972    1099    3994    4971 
   Size:        972     127    2895     977

  Fragments arranged by size: 

                2895     977     972     127
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1677    4734    4860    4971 
   Size:       1677    3057     126     111

  Fragments arranged by size: 

                3057    1677     126     111
 
EcoRI G'AATT_C

Cuts at:      0     692    1540    2290    4971 
   Size:        692     848     750    2681

  Fragments arranged by size: 

                2681     848     750     692
 
EcoRV GAT'ATC

Cuts at:      0     414    1560    2742    4971 
   Size:        414    1146    1182    2229

  Fragments arranged by size: 

                2229    1182    1146     414
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     543    1887    4569    4735    4971 
   Size:        543    1344    2682     166     236

  Fragments arranged by size: 

                2682    1344     543     236     166
 
HaeII r_GCGC'y

Cuts at:      0    2472    3364    4971 
   Size:       2472     892    1607

  Fragments arranged by size: 

                2472    1607     892
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1099    1131    1354    1386    3126    3158    3232    3264 
   Size:       1099      32     223      32    1740      32      74      32

Cuts at:   3264    3294    3326    3835    3867    4268    4300    4877    4909 
   Size:         30      32     509      32     401      32     577      32

Cuts at:   4909    4971 
   Size:         62

  Fragments arranged by size: 

                1740    1099     577     509     401     223      74      62
                  32      32      32      32      32      32      32      32
                  30
 
HincII GTy'rAC

Cuts at:      0      21     525    4929    4971 
   Size:         21     504    4404      42

  Fragments arranged by size: 

                4404     504      42      21
 
HindIII A'AGCT_T

Cuts at:      0    1565    2909    4971 
   Size:       1565    1344    2062

  Fragments arranged by size: 

                2062    1565    1344
 
KpnI G_GTAC'C

Cuts at:      0    4485    4971 
   Size:       4485     486
 
MfeI C'AATT_G

Cuts at:      0    1432    4971 
   Size:       1432    3539
 
MluI A'CGCG_T

Cuts at:      0    3035    4971 
   Size:       3035    1936
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     545    1787    1862    2885    3065    3908    4773    4804 
   Size:        545    1242      75    1023     180     843     865      31

Cuts at:   4804    4971 
   Size:        167

  Fragments arranged by size: 

                1242    1023     865     843     545     180     167      75
                  31
 
MscI TGG'CCA

Cuts at:      0    2230    2959    3191    4971 
   Size:       2230     729     232    1780

  Fragments arranged by size: 

                2230    1780     729     232
 
MslI CAynn'nnrTG

Cuts at:      0     583    2417    2507    3938    4971 
   Size:        583    1834      90    1431    1033

  Fragments arranged by size: 

                1834    1431    1033     583      90
 
MspA1I CmG'CkG

Cuts at:      0    1251    1795    2220    2715    4476    4971 
   Size:       1251     544     425     495    1761     495

  Fragments arranged by size: 

                1761    1251     544     495     495     425
 
NcoI C'CATG_G

Cuts at:      0     365    4971 
   Size:        365    4606
 
NheI G'CTAG_C

Cuts at:      0    3363    4971 
   Size:       3363    1608
 
NspI r_CATG'y

Cuts at:      0     170     435     655    1528    3414    3703    4971 
   Size:        170     265     220     873    1886     289    1268

  Fragments arranged by size: 

                1886    1268     873     289     265     220     170
 
PacI TTA_AT'TAA

Cuts at:      0    1634    4971 
   Size:       1634    3337
 
PasI CC'CwG_GG

Cuts at:      0     996    4971 
   Size:        996    3975
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2097    2625    2715    4971 
   Size:       2097     528      90    2256

  Fragments arranged by size: 

                2256    2097     528      90
 
PfoI T'CCnGG_A

Cuts at:      0      59    1781    2299    2495    4971 
   Size:         59    1722     518     196    2476

  Fragments arranged by size: 

                2476    1722     518     196      59
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     238     270    1316    1348    4971 
   Size:        238      32    1046      32    3623

  Fragments arranged by size: 

                3623    1046     238      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    4734    4860    4971 
   Size:       4734     126     111

  Fragments arranged by size: 

                4734     126     111
 
PstI C_TGCA'G

Cuts at:      0      84     986    1016    1100    1115    1310    1676    1695 
   Size:         84     902      30      84      15     195     366      19

Cuts at:   1695    2258    2489    4871    4971 
   Size:        563     231    2382     100

  Fragments arranged by size: 

                2382     902     563     366     231     195     100      84
                  84      30      19      15
 
PvuII CAG'CTG

Cuts at:      0    1251    1795    2220    2715    4971 
   Size:       1251     544     425     495    2256

  Fragments arranged by size: 

                2256    1251     544     495     425
 
SacI G_AGCT'C

Cuts at:      0    1349    4971 
   Size:       1349    3622
 
SapI GCTCTTCn'nnn_

Cuts at:      0     746    4971 
   Size:        746    4225
 
ScaI AGT'ACT

Cuts at:      0    2491    3850    4971 
   Size:       2491    1359    1121

  Fragments arranged by size: 

                2491    1359    1121
 
SexAI A'CCwGG_T

Cuts at:      0    1586    4971 
   Size:       1586    3385
 
SfcI C'TryA_G

Cuts at:      0      80     407     982    1012    1096    1111    1306    1672 
   Size:         80     327     575      30      84      15     195     366

Cuts at:   1672    1681    1691    2254    2485    4867    4873    4971 
   Size:          9      10     563     231    2382       6      98

  Fragments arranged by size: 

                2382     575     563     366     327     231     195      98
                  84      80      30      15      10       9       6
 
SmlI C'TyrA_G

Cuts at:      0    1702    2872    3741    4971 
   Size:       1702    1170     869    1230

  Fragments arranged by size: 

                1702    1230    1170     869
 
SpeI A'CTAG_T

Cuts at:      0    4506    4971 
   Size:       4506     465
 
SphI G_CATG'C

Cuts at:      0     170    3703    4971 
   Size:        170    3533    1268

  Fragments arranged by size: 

                3533    1268     170
 
SspI AAT'ATT

Cuts at:      0    1464    4971 
   Size:       1464    3507
 
StuI AGG'CCT

Cuts at:      0     488    2240    4971 
   Size:        488    1752    2731

  Fragments arranged by size: 

                2731    1752     488
 
StyI C'CwwG_G

Cuts at:      0     171     365     758    4642    4971 
   Size:        171     194     393    3884     329

  Fragments arranged by size: 

                3884     393     329     194     171
 
SwaI ATTT'AAAT

Cuts at:      0    4043    4971 
   Size:       4043     928
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1351    1355    1864    2043    3928    4971 
   Size:       1351       4     509     179    1885    1043

  Fragments arranged by size: 

                1885    1351    1043     509     179       4
 
TatI w'GTAC_w

Cuts at:      0    2489    3848    4971 
   Size:       2489    1359    1123

  Fragments arranged by size: 

                2489    1359    1123
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2744    2776    4971 
   Size:       2744      32    2195

  Fragments arranged by size: 

                2744    2195      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1316    4971 
   Size:       1316    3655
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     198    3000    4971 
   Size:        198    2802    1971

  Fragments arranged by size: 

                2802    1971     198
 
XmnI GAAnn'nnTTC

Cuts at:      0     250    4971 
   Size:        250    4721
 
ZraI GAC'GTC

Cuts at:      0     106    4971 
   Size:        106    4865
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AlfI     AloI    AlwNI     ApoI     AseI     AvaI    AvrII
    BamHI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI     BclI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI     BtsI
     ClaI     DraI   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I
   HincII  HindIII     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NheI     NspI     PacI     PasI    PflMI     PfoI
     PpiI    PpuMI     PstI    PvuII     SacI     SapI     ScaI    SexAI
     SfcI     SmlI     SpeI     SphI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AgeI     AhdI     AleI     ApaI    ApaLI     AscI    AsiSI     BaeI
     BbeI    BfrBI     BglI     BplI    BsaAI     BsgI    BsiWI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstEII  BstZ17I    BtgZI    CspCI
     EciI     FseI     FspI    FspAI     HpaI     KasI     NaeI     NarI
     NdeI   NgoMIV     NotI     NruI     NsiI     PciI     PmeI     PmlI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SrfI     XbaI     XhoI     XmaI
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