Restriction map for pF1KSDF0400
 (Linear) (Six-base) MAPSORT of: FLJ00400.seq  Check: 1496  from: 1  to: 2178



FLJ00400.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:19  ..

AclI AA'CG_TT

Cuts at:      0    1235    2178 
   Size:       1235     943
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1159    1564    1779    2178 
   Size:       1159     405     215     399

  Fragments arranged by size: 

                1159     405     399     215
 
AgeI A'CCGG_T

Cuts at:      0    1361    2178 
   Size:       1361     817
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     781    2178 
   Size:        781    1397
 
AlwNI CAG_nnn'CTG

Cuts at:      0     249    1014    2178 
   Size:        249     765    1164

  Fragments arranged by size: 

                1164     765     249
 
ApaI G_GGCC'C

Cuts at:      0      36     870    1981    2077    2178 
   Size:         36     834    1111      96     101

  Fragments arranged by size: 

                1111     834     101      96      36
 
ApoI r'AATT_y

Cuts at:      0     314    2178 
   Size:        314    1864
 
AvaI C'yCGr_G

Cuts at:      0     254     863    1962    2049    2178 
   Size:        254     609    1099      87     129

  Fragments arranged by size: 

                1099     609     254     129      87
 
BanI G'GyrC_C

Cuts at:      0      42     224     717    1919    2128    2178 
   Size:         42     182     493    1202     209      50

  Fragments arranged by size: 

                1202     493     209     182      50      42
 
BanII G_rGCy'C

Cuts at:      0      36     164     269     870     923    1748    1786    1981 
   Size:         36     128     105     601      53     825      38     195

Cuts at:   1981    2077    2178 
   Size:         96     101

  Fragments arranged by size: 

                 825     601     195     128     105     101      96      53
                  38      36
 
BbeI G_GCGC'C

Cuts at:      0     228     721    2178 
   Size:        228     493    1457

  Fragments arranged by size: 

                1457     493     228
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     697     729    1494    2178 
   Size:        697      32     765     684

  Fragments arranged by size: 

                 765     697     684      32
 
BbvCI CC'TCA_GC

Cuts at:      0     619    2178 
   Size:        619    1559
 
BglI GCCn_nnn'nGGC

Cuts at:      0     305     874    1347    2178 
   Size:        305     569     473     831

  Fragments arranged by size: 

                 831     569     473     305
 
BlpI GC'TnA_GC

Cuts at:      0     364    1819    2178 
   Size:        364    1455     359

  Fragments arranged by size: 

                1455     364     359
 
Bme1580I G_kGCm'C

Cuts at:      0      36     460     870    1643    1770    1981    2077    2133 
   Size:         36     424     410     773     127     211      96      56

Cuts at:   2133    2178 
   Size:         45

  Fragments arranged by size: 

                 773     424     410     211     127      96      56      45
                  36
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     603    1988    2178 
   Size:        603    1385     190

  Fragments arranged by size: 

                1385     603     190
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436     844     991    1102    1536    1566    2178 
   Size:        436     408     147     111     434      30     612

  Fragments arranged by size: 

                 612     436     434     408     147     111      30
 
Bpu10I CC'TnA_GC

Cuts at:      0     619    2178 
   Size:        619    1559
 
BsaHI Gr'CG_yC

Cuts at:      0     100     225     718    2178 
   Size:        100     125     493    1460

  Fragments arranged by size: 

                1460     493     125     100
 
BsaWI w'CCGG_w

Cuts at:      0    1361    1883    2178 
   Size:       1361     522     295

  Fragments arranged by size: 

                1361     522     295
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      46      76     979    1009    1264    1294    1768    1798 
   Size:         46      30     903      30     255      30     474      30

Cuts at:   1798    1921    1951    1978    2008    2178 
   Size:        123      30      27      30     170

  Fragments arranged by size: 

                 903     474     255     170     123      46      30      30
                  30      30      30      30      27
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      70     607     631     732     841    1000    1288    1297 
   Size:         70     537      24     101     109     159     288       9

Cuts at:   1297    1414    1603    1662    1798    2178 
   Size:        117     189      59     136     380

  Fragments arranged by size: 

                 537     380     288     189     159     136     117     109
                 101      70      59      24       9
 
BseYI C'CCAG_C

Cuts at:      0     699    1253    2161    2178 
   Size:        699     554     908      17

  Fragments arranged by size: 

                 908     699     554      17
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     787    2178 
   Size:        193     594    1391

  Fragments arranged by size: 

                1391     594     193
 
BsiEI CG_ry'CG

Cuts at:      0    2071    2178 
   Size:       2071     107
 
BsiHKAI G_wGCw'C

Cuts at:      0    1613    2178 
   Size:       1613     565
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1173    1848    2178 
   Size:       1173     675     330

  Fragments arranged by size: 

                1173     675     330
 
Bsp1286I G_dGCh'C

Cuts at:      0      36     164     269     460     870     923    1613    1643 
   Size:         36     128     105     191     410      53     690      30

Cuts at:   1643    1748    1770    1786    1981    2077    2133    2178 
   Size:        105      22      16     195      96      56      45

  Fragments arranged by size: 

                 690     410     195     191     128     105     105      96
                  56      53      45      36      30      22      16
 
BspEI T'CCGG_A

Cuts at:      0    1883    2178 
   Size:       1883     295
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     368     905    1022    1893    2178 
   Size:        368     537     117     871     285

  Fragments arranged by size: 

                 871     537     368     285     117
 
BsrBI CCG'CTC

Cuts at:      0      30      81    2178 
   Size:         30      51    2097

  Fragments arranged by size: 

                2097      51      30
 
BsrFI r'CCGG_y

Cuts at:      0     203    1361    1373    2178 
   Size:        203    1158      12     805

  Fragments arranged by size: 

                1158     805     203      12
 
BsrGI T'GTAC_A

Cuts at:      0     593    1280    2178 
   Size:        593     687     898

  Fragments arranged by size: 

                 898     687     593
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     180    2178 
   Size:        180    1998
 
BstEII G'GTnAC_C

Cuts at:      0    1332    1449    2178 
   Size:       1332     117     729

  Fragments arranged by size: 

                1332     729     117
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     460    2178 
   Size:        460    1718
 
BtgI C'CryG_G

Cuts at:      0      10     164    1265    1313    2065    2093    2178 
   Size:         10     154    1101      48     752      28      85

  Fragments arranged by size: 

                1101     752     154      85      48      28      10
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1014    2178 
   Size:       1014    1164
 
BtsI GCAGTG_nn'

Cuts at:      0    1643    2178 
   Size:       1643     535
 
DraIII CAC_nnn'GTG

Cuts at:      0    1926    2178 
   Size:       1926     252
 
EaeI y'GGCC_r

Cuts at:      0    1145    1758    2068    2178 
   Size:       1145     613     310     110

  Fragments arranged by size: 

                1145     613     310     110
 
EagI C'GGCC_G

Cuts at:      0    2068    2178 
   Size:       2068     110
 
EarI CTCTTCn'nnn_

Cuts at:      0     307     556    1471    1513    1696    2178 
   Size:        307     249     915      42     183     482

  Fragments arranged by size: 

                 915     482     307     249     183      42
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     403     592     681    2130    2178 
   Size:        403     189      89    1449      48

  Fragments arranged by size: 

                1449     403     189      89      48
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436     844     991    1102    1159    1536    1564    1566 
   Size:        436     408     147     111      57     377      28       2

Cuts at:   1566    1779    2178 
   Size:        213     399

  Fragments arranged by size: 

                 436     408     399     377     213     147     111      57
                  28       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     232     425    1927    2178 
   Size:        232     193    1502     251

  Fragments arranged by size: 

                1502     251     232     193
 
EcoO109I rG'GnC_Cy

Cuts at:      0      33     258     297     430     530    1051    1932    1978 
   Size:         33     225      39     133     100     521     881      46

Cuts at:   1978    2061    2074    2178 
   Size:         83      13     104

  Fragments arranged by size: 

                 881     521     225     133     104     100      83      46
                  39      33      13
 
EcoRI G'AATT_C

Cuts at:      0     314    2178 
   Size:        314    1864
 
HaeII r_GCGC'y

Cuts at:      0     141     204     228     283     721     731     947    2178 
   Size:        141      63      24      55     438      10     216    1231

  Fragments arranged by size: 

                1231     438     216     141      63      55      24      10
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1351    1383    1921    1953    2178 
   Size:       1351      32     538      32     225

  Fragments arranged by size: 

                1351     538     225      32      32
 
HincII GTy'rAC

Cuts at:      0     906    1041    1110    2178 
   Size:        906     135      69    1068

  Fragments arranged by size: 

                1068     906     135      69
 
HindIII A'AGCT_T

Cuts at:      0     384    2000    2178 
   Size:        384    1616     178

  Fragments arranged by size: 

                1616     384     178
 
KasI G'GCGC_C

Cuts at:      0     224     717    2178 
   Size:        224     493    1461

  Fragments arranged by size: 

                1461     493     224
 
MscI TGG'CCA

Cuts at:      0    1147    1760    2178 
   Size:       1147     613     418

  Fragments arranged by size: 

                1147     613     418
 
MslI CAynn'nnrTG

Cuts at:      0     763    1456    2178 
   Size:        763     693     722

  Fragments arranged by size: 

                 763     722     693
 
MspA1I CmG'CkG

Cuts at:      0     153     166     192     246     627     846    1080    1214 
   Size:        153      13      26      54     381     219     234     134

Cuts at:   1214    1893    2067    2095    2165    2178 
   Size:        679     174      28      70      13

  Fragments arranged by size: 

                 679     381     234     219     174     153     134      70
                  54      28      26      13      13
 
NaeI GCC'GGC

Cuts at:      0     205    2178 
   Size:        205    1973
 
NarI GG'CG_CC

Cuts at:      0     225     718    2178 
   Size:        225     493    1460

  Fragments arranged by size: 

                1460     493     225
 
NgoMIV G'CCGG_C

Cuts at:      0     203    2178 
   Size:        203    1975
 
NruI TCG'CGA

Cuts at:      0    1941    2178 
   Size:       1941     237
 
NspI r_CATG'y

Cuts at:      0     517    2178 
   Size:        517    1661
 
PasI CC'CwG_GG

Cuts at:      0      37    1021    1173    1644    2178 
   Size:         37     984     152     471     534

  Fragments arranged by size: 

                 984     534     471     152      37
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     953    1779    2041    2178 
   Size:        953     826     262     137

  Fragments arranged by size: 

                 953     826     262     137
 
PfoI T'CCnGG_A

Cuts at:      0    1724    2178 
   Size:       1724     454
 
PpuMI rG'GwC_Cy

Cuts at:      0    1051    1932    2178 
   Size:       1051     881     246

  Fragments arranged by size: 

                1051     881     246
 
PspOMI G'GGCC_C

Cuts at:      0      32     866    1977    2073    2178 
   Size:         32     834    1111      96     105

  Fragments arranged by size: 

                1111     834     105      96      32
 
PstI C_TGCA'G

Cuts at:      0    1638    2178 
   Size:       1638     540
 
PvuII CAG'CTG

Cuts at:      0     153     846    1080    1214    1893    2178 
   Size:        153     693     234     134     679     285

  Fragments arranged by size: 

                 693     679     285     234     153     134
 
SacII CC_GC'GG

Cuts at:      0     167    2068    2096    2178 
   Size:        167    1901      28      82

  Fragments arranged by size: 

                1901     167      82      28
 
SanDI GG'GwC_CC

Cuts at:      0    1051    2178 
   Size:       1051    1127
 
ScaI AGT'ACT

Cuts at:      0     967    2178 
   Size:        967    1211
 
SexAI A'CCwGG_T

Cuts at:      0    1922    2178 
   Size:       1922     256
 
SfcI C'TryA_G

Cuts at:      0    1634    2178 
   Size:       1634     544
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     874    2178 
   Size:        874    1304
 
SfoI GGC'GCC

Cuts at:      0     226     719    2178 
   Size:        226     493    1459

  Fragments arranged by size: 

                1459     493     226
 
SgrAI Cr'CCGG_yG

Cuts at:      0     203    2178 
   Size:        203    1975
 
SmaI CCC'GGG

Cuts at:      0     256     865    2051    2178 
   Size:        256     609    1186     127

  Fragments arranged by size: 

                1186     609     256     127
 
StyI C'CwwG_G

Cuts at:      0     261     731    1740    2178 
   Size:        261     470    1009     438

  Fragments arranged by size: 

                1009     470     438     261
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     308    2178 
   Size:        308    1870
 
TatI w'GTAC_w

Cuts at:      0     593     965    1280    1532    2178 
   Size:        593     372     315     252     646

  Fragments arranged by size: 

                 646     593     372     315     252
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      46      78    2178 
   Size:         46      32    2100

  Fragments arranged by size: 

                2100      46      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     478    1422    2178 
   Size:        478     944     756

  Fragments arranged by size: 

                 944     756     478
 
XmaI C'CCGG_G

Cuts at:      0     254     863    2049    2178 
   Size:        254     609    1186     129

  Fragments arranged by size: 

                1186     609     254     129
 

 Enzymes that do cut:

     AclI     AcuI     AgeI     AlfI    AlwNI     ApaI     ApoI     AvaI
     BanI    BanII     BbeI     BbsI    BbvCI     BglI     BlpI Bme1580I
     BplI     BpmI   Bpu10I    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI
    BsrFI    BsrGI   BstAPI   BstEII    BstXI     BtgI    BtgZI     BtsI
   DraIII     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I
    EcoRI    HaeII    Hin4I   HincII  HindIII     KasI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV     NruI     NspI     PasI    PflMI
     PfoI    PpuMI   PspOMI     PstI    PvuII    SacII    SanDI     ScaI
    SexAI     SfcI     SfiI     SfoI    SgrAI     SmaI     StyI    TaqII
     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AhdI
     AleI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI     BmrI
     BmtI    BpuEI     BsaI    BsaAI    BsaBI    BsiWI     BsmI    BspHI
    BsrDI   BssHII    BssSI    BstBI    BstYI  BstZ17I   Bsu36I     ClaI
    CspCI     DraI     DrdI  EcoICRI    EcoRV     FalI     FseI     FspI
    FspAI     HpaI     KpnI     MfeI     MluI     MmeI     NcoI     NdeI
     NheI     NotI     NsiI     PacI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI     SalI
     SapI     SbfI     SgfI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI  Tth111I     XbaI     XhoI     XmnI     ZraI
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