Restriction map for pF1KSDF0390
 (Linear) (Six-base) MAPSORT of: FLJ00390.seq  Check: 4064  from: 1  to: 825



FLJ00390.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:19  ..

AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     775     807     825 
   Size:        775      32      18

  Fragments arranged by size: 

                 775      32      18
 
AlwNI CAG_nnn'CTG

Cuts at:      0      36     303     333     405     815     825 
   Size:         36     267      30      72     410      10

  Fragments arranged by size: 

                 410     267      72      36      30      10
 
ApaI G_GGCC'C

Cuts at:      0     132     825 
   Size:        132     693
 
ApoI r'AATT_y

Cuts at:      0     739     825 
   Size:        739      86
 
AvaI C'yCGr_G

Cuts at:      0     318     415     536     825 
   Size:        318      97     121     289

  Fragments arranged by size: 

                 318     289     121      97
 
BanI G'GyrC_C

Cuts at:      0     149     825 
   Size:        149     676
 
BanII G_rGCy'C

Cuts at:      0     132     248     825 
   Size:        132     116     577

  Fragments arranged by size: 

                 577     132     116
 
BglI GCCn_nnn'nGGC

Cuts at:      0     206     541     825 
   Size:        206     335     284

  Fragments arranged by size: 

                 335     284     206
 
Bme1580I G_kGCm'C

Cuts at:      0     132     297     636     825 
   Size:        132     165     339     189

  Fragments arranged by size: 

                 339     189     165     132
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     514     825 
   Size:        127     387     311

  Fragments arranged by size: 

                 387     311     127
 
BsaHI Gr'CG_yC

Cuts at:      0     263     590     825 
   Size:        263     327     235

  Fragments arranged by size: 

                 327     263     235
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     125     155     688     718     825 
   Size:        125      30     533      30     107

  Fragments arranged by size: 

                 533     125     107      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     511     696     802     825 
   Size:        511     185     106      23

  Fragments arranged by size: 

                 511     185     106      23
 
BseYI C'CCAG_C

Cuts at:      0      68     103     125     825 
   Size:         68      35      22     700

  Fragments arranged by size: 

                 700      68      35      22
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     474     825 
   Size:        474     351
 
BsmI GAATG_Cn'

Cuts at:      0     114     825 
   Size:        114     711
 
Bsp1286I G_dGCh'C

Cuts at:      0     132     248     297     636     825 
   Size:        132     116      49     339     189

  Fragments arranged by size: 

                 339     189     132     116      49
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     714     825 
   Size:        714     111
 
BsrDI GCAATG_nn'

Cuts at:      0     477     825 
   Size:        477     348
 
BsrGI T'GTAC_A

Cuts at:      0     620     825 
   Size:        620     205
 
BstYI r'GATC_y

Cuts at:      0      15     373     489     825 
   Size:         15     358     116     336

  Fragments arranged by size: 

                 358     336     116      15
 
BtgI C'CryG_G

Cuts at:      0     266     278     309     612     825 
   Size:        266      12      31     303     213

  Fragments arranged by size: 

                 303     266     213      31      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     534     825 
   Size:        534     291
 
BtsI GCAGTG_nn'

Cuts at:      0      10      97     585     657     825 
   Size:         10      87     488      72     168

  Fragments arranged by size: 

                 488     168      87      72      10
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     724     825 
   Size:        724     101
 
EaeI y'GGCC_r

Cuts at:      0     335     407     627     797     825 
   Size:        335      72     220     170      28

  Fragments arranged by size: 

                 335     220     170      72      28
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     337     451     751     825 
   Size:        337     114     300      74

  Fragments arranged by size: 

                 337     300     114      74
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     514     825 
   Size:        127     387     311

  Fragments arranged by size: 

                 387     311     127
 
EcoO109I rG'GnC_Cy

Cuts at:      0      33     129     199     314     330     825 
   Size:         33      96      70     115      16     495

  Fragments arranged by size: 

                 495     115      96      70      33      16
 
EcoRI G'AATT_C

Cuts at:      0     739     825 
   Size:        739      86
 
FspI TGC'GCA

Cuts at:      0     649     825 
   Size:        649     176
 
HaeII r_GCGC'y

Cuts at:      0     514     825 
   Size:        514     311
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     474     506     825 
   Size:        474      32     319

  Fragments arranged by size: 

                 474     319      32
 
MscI TGG'CCA

Cuts at:      0     337     409     799     825 
   Size:        337      72     390      26

  Fragments arranged by size: 

                 390     337      72      26
 
MspA1I CmG'CkG

Cuts at:      0     125     236     311     479     556     614     758     822 
   Size:        125     111      75     168      77      58     144      64

Cuts at:    822     825 
   Size:          3

  Fragments arranged by size: 

                 168     144     125     111      77      75      64      58
                   3
 
NcoI C'CATG_G

Cuts at:      0     278     825 
   Size:        278     547
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     333     815     825 
   Size:        333     482      10

  Fragments arranged by size: 

                 482     333      10
 
PfoI T'CCnGG_A

Cuts at:      0     689     743     825 
   Size:        689      54      82

  Fragments arranged by size: 

                 689      82      54
 
PspOMI G'GGCC_C

Cuts at:      0     128     825 
   Size:        128     697
 
PvuII CAG'CTG

Cuts at:      0     125     236     556     822     825 
   Size:        125     111     320     266       3

  Fragments arranged by size: 

                 320     266     125     111       3
 
SacII CC_GC'GG

Cuts at:      0     312     615     825 
   Size:        312     303     210

  Fragments arranged by size: 

                 312     303     210
 
SfcI C'TryA_G

Cuts at:      0     753     825 
   Size:        753      72
 
SmaI CCC'GGG

Cuts at:      0     538     825 
   Size:        538     287
 
StyI C'CwwG_G

Cuts at:      0     278     641     825 
   Size:        278     363     184

  Fragments arranged by size: 

                 363     278     184
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      92     679     825 
   Size:         92     587     146

  Fragments arranged by size: 

                 587     146      92
 
TatI w'GTAC_w

Cuts at:      0     620     825 
   Size:        620     205
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     125     157     825 
   Size:        125      32     668

  Fragments arranged by size: 

                 668     125      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     589     825 
   Size:        589     236
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     255     417     825 
   Size:        255     162     408

  Fragments arranged by size: 

                 408     255     162
 
XmaI C'CCGG_G

Cuts at:      0     536     825 
   Size:        536     289
 

 Enzymes that do cut:

     AloI    AlwNI     ApaI     ApoI     AvaI     BanI    BanII     BglI
 Bme1580I     BpmI    BsaHI    BsaXI    BseRI    BseYI     BsgI     BsmI
 Bsp1286I    BspMI    BsrDI    BsrGI    BstYI     BtgI    BtgZI     BtsI
     DrdI     EaeI     EciI  Eco57MI EcoO109I    EcoRI     FspI    HaeII
    Hin4I     MscI   MspA1I     NcoI    PflMI     PfoI   PspOMI    PvuII
    SacII     SfcI     SmaI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsiEI
  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     ClaI
    CspCI     DraI   DraIII     EagI     EarI  EcoICRI    EcoNI    EcoRV
     FalI     FseI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     XbaI     XhoI
     XmnI     ZraI
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