Restriction map for pF1KSDF0380
 (Linear) (Six-base) MAPSORT of: FLJ00380.seq  Check: 8391  from: 1  to: 2316



FLJ00380.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     796    2316 
   Size:        796    1520
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31    1116    1499    2316 
   Size:         31    1085     383     817

  Fragments arranged by size: 

                1085     817     383      31
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2148    2316 
   Size:       2148     168
 
ApaI G_GGCC'C

Cuts at:      0     137     267    2316 
   Size:        137     130    2049

  Fragments arranged by size: 

                2049     137     130
 
ApoI r'AATT_y

Cuts at:      0    1414    2316 
   Size:       1414     902
 
AvaI C'yCGr_G

Cuts at:      0     370    1138    1853    2316 
   Size:        370     768     715     463

  Fragments arranged by size: 

                 768     715     463     370
 
BamHI G'GATC_C

Cuts at:      0    1671    2316 
   Size:       1671     645
 
BanI G'GyrC_C

Cuts at:      0     282     723    1737    1976    2316 
   Size:        282     441    1014     239     340

  Fragments arranged by size: 

                1014     441     340     282     239
 
BanII G_rGCy'C

Cuts at:      0     137     267     866    1247    1854    2316 
   Size:        137     130     599     381     607     462

  Fragments arranged by size: 

                 607     599     462     381     137     130
 
BbeI G_GCGC'C

Cuts at:      0    1980    2316 
   Size:       1980     336
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      78     208    2316 
   Size:         78     130    2108

  Fragments arranged by size: 

                2108     130      78
 
BglII A'GATC_T

Cuts at:      0     516     945    2316 
   Size:        516     429    1371

  Fragments arranged by size: 

                1371     516     429
 
BlpI GC'TnA_GC

Cuts at:      0    1679    1954    2316 
   Size:       1679     275     362

  Fragments arranged by size: 

                1679     362     275
 
Bme1580I G_kGCm'C

Cuts at:      0     137     267    1017    1749    1782    2316 
   Size:        137     130     750     732      33     534

  Fragments arranged by size: 

                 750     732     534     137     130      33
 
BmgBI CAC'GTC

Cuts at:      0      81    2316 
   Size:         81    2235
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     131     153     478     710    1736    1769    2198    2316 
   Size:        131      22     325     232    1026      33     429     118

  Fragments arranged by size: 

                1026     429     325     232     131     118      33      22
            
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      22    1043    1426    1431    1723    2065    2203    2316 
   Size:         22    1021     383       5     292     342     138     113

  Fragments arranged by size: 

                1021     383     342     292     138     113      22       5
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1468    2316 
   Size:       1468     848
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1768    2316 
   Size:       1768     548
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     314    1019    1585    2316 
   Size:        314     705     566     731

  Fragments arranged by size: 

                 731     705     566     314
 
BsaAI yAC'GTr

Cuts at:      0    1710    2316 
   Size:       1710     606
 
BsaBI GATnn'nnATC

Cuts at:      0     526     548    2316 
   Size:        526      22    1768

  Fragments arranged by size: 

                1768     526      22
 
BsaHI Gr'CG_yC

Cuts at:      0    1096    1977    2316 
   Size:       1096     881     339

  Fragments arranged by size: 

                1096     881     339
 
BsaWI w'CCGG_w

Cuts at:      0    2306    2316 
   Size:       2306      10
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     238     884    1423    1494    1497    1921    2236    2316 
   Size:        238     646     539      71       3     424     315      80

  Fragments arranged by size: 

                 646     539     424     315     238      80      71       3
            
 
BseYI C'CCAG_C

Cuts at:      0     559    1192    1259    1400    1949    2316 
   Size:        559     633      67     141     549     367

  Fragments arranged by size: 

                 633     559     549     367     141      67
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     701    1411    2316 
   Size:        701     710     905

  Fragments arranged by size: 

                 905     710     701
 
BsiHKAI G_wGCw'C

Cuts at:      0    1039    1709    1847    2316 
   Size:       1039     670     138     469

  Fragments arranged by size: 

                1039     670     469     138
 
BsmI GAATG_Cn'

Cuts at:      0     810    2316 
   Size:        810    1506
 
Bsp1286I G_dGCh'C

Cuts at:      0     137     267     866    1017    1039    1247    1709    1749 
   Size:        137     130     599     151      22     208     462      40

Cuts at:   1749    1782    1847    1854    2316 
   Size:         33      65       7     462

  Fragments arranged by size: 

                 599     462     462     208     151     137     130      65
                  40      33      22       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     689     796    1772    1904    2072    2316 
   Size:        689     107     976     132     168     244

  Fragments arranged by size: 

                 976     689     244     168     132     107
 
BsrGI T'GTAC_A

Cuts at:      0    1007    2316 
   Size:       1007    1309
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1744    2316 
   Size:       1744     572
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1267    1749    2316 
   Size:       1267     482     567

  Fragments arranged by size: 

                1267     567     482
 
BstYI r'GATC_y

Cuts at:      0     516     945    1599    1671    1865    2316 
   Size:        516     429     654      72     194     451

  Fragments arranged by size: 

                 654     516     451     429     194      72
 
Bsu36I CC'TnA_GG

Cuts at:      0     172     769    2316 
   Size:        172     597    1547

  Fragments arranged by size: 

                1547     597     172
 
BtgI C'CryG_G

Cuts at:      0     974    2316 
   Size:        974    1342
 
BtsI GCAGTG_nn'

Cuts at:      0     233     851     943    2244    2316 
   Size:        233     618      92    1301      72

  Fragments arranged by size: 

                1301     618     233      92      72
 
DraIII CAC_nnn'GTG

Cuts at:      0    1744    1777    2316 
   Size:       1744      33     539

  Fragments arranged by size: 

                1744     539      33
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     750    2239    2316 
   Size:        750    1489      77

  Fragments arranged by size: 

                1489     750      77
 
EaeI y'GGCC_r

Cuts at:      0    1160    1364    2264    2316 
   Size:       1160     204     900      52

  Fragments arranged by size: 

                1160     900     204      52
 
EarI CTCTTCn'nnn_

Cuts at:      0     755     878     902    1135    1252    1500    1515    1521 
   Size:        755     123      24     233     117     248      15       6

Cuts at:   1521    1548    1601    2316 
   Size:         27      53     715

  Fragments arranged by size: 

                 755     715     248     233     123     117      53      27
                  24      15       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      22      31    1043    1116    1426    1431    1499    1723 
   Size:         22       9    1012      73     310       5      68     224

Cuts at:   1723    2065    2203    2316 
   Size:        342     138     113

  Fragments arranged by size: 

                1012     342     310     224     138     113      73      68
                  22       9       5
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     253    1090    2152    2316 
   Size:        253     837    1062     164

  Fragments arranged by size: 

                1062     837     253     164
 
EcoO109I rG'GnC_Cy

Cuts at:      0     133     263     264    2061    2316 
   Size:        133     130       1    1797     255

  Fragments arranged by size: 

                1797     255     133     130       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      36     784    2316 
   Size:         36     748    1532

  Fragments arranged by size: 

                1532     748      36
 
HaeII r_GCGC'y

Cuts at:      0    1980    2316 
   Size:       1980     336
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     937     969    1411    1443    1584    1616    2316 
   Size:        937      32     442      32     141      32     700

  Fragments arranged by size: 

                 937     700     442     141      32      32      32
 
KasI G'GCGC_C

Cuts at:      0    1976    2316 
   Size:       1976     340
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1319    2316 
   Size:       1319     997
 
MscI TGG'CCA

Cuts at:      0    1162    1366    2316 
   Size:       1162     204     950

  Fragments arranged by size: 

                1162     950     204
 
MslI CAynn'nnrTG

Cuts at:      0     964     979    2316 
   Size:        964      15    1337

  Fragments arranged by size: 

                1337     964      15
 
MspA1I CmG'CkG

Cuts at:      0    1404    1953    2316 
   Size:       1404     549     363

  Fragments arranged by size: 

                1404     549     363
 
NarI GG'CG_CC

Cuts at:      0    1977    2316 
   Size:       1977     339
 
NcoI C'CATG_G

Cuts at:      0     974    2316 
   Size:        974    1342
 
PasI CC'CwG_GG

Cuts at:      0    1101    2316 
   Size:       1101    1215
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     490    2316 
   Size:        490    1826
 
PfoI T'CCnGG_A

Cuts at:      0     116    1020    1817    2316 
   Size:        116     904     797     499

  Fragments arranged by size: 

                 904     797     499     116
 
PmlI CAC'GTG

Cuts at:      0    1710    2316 
   Size:       1710     606
 
PpuMI rG'GwC_Cy

Cuts at:      0    2061    2316 
   Size:       2061     255
 
PshAI GACnn'nnGTC

Cuts at:      0     581    2316 
   Size:        581    1735
 
PspOMI G'GGCC_C

Cuts at:      0     133     263    2316 
   Size:        133     130    2053

  Fragments arranged by size: 

                2053     133     130
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1633    1665    2316 
   Size:       1633      32     651

  Fragments arranged by size: 

                1633     651      32
 
PstI C_TGCA'G

Cuts at:      0    1114    2069    2316 
   Size:       1114     955     247

  Fragments arranged by size: 

                1114     955     247
 
PvuII CAG'CTG

Cuts at:      0    1404    1953    2316 
   Size:       1404     549     363

  Fragments arranged by size: 

                1404     549     363
 
SapI GCTCTTCn'nnn_

Cuts at:      0     878    1252    2316 
   Size:        878     374    1064

  Fragments arranged by size: 

                1064     878     374
 
SfcI C'TryA_G

Cuts at:      0     473     579    1044    1110    1575    2065    2316 
   Size:        473     106     465      66     465     490     251

  Fragments arranged by size: 

                 490     473     465     465     251     106      66
 
SfoI GGC'GCC

Cuts at:      0    1978    2316 
   Size:       1978     338
 
SmlI C'TyrA_G

Cuts at:      0     370    1783    2316 
   Size:        370    1413     533

  Fragments arranged by size: 

                1413     533     370
 
StuI AGG'CCT

Cuts at:      0     362    1396    1433    2316 
   Size:        362    1034      37     883

  Fragments arranged by size: 

                1034     883     362      37
 
StyI C'CwwG_G

Cuts at:      0     182     974    2316 
   Size:        182     792    1342

  Fragments arranged by size: 

                1342     792     182
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1755    1788    2316 
   Size:       1755      33     528

  Fragments arranged by size: 

                1755     528      33
 
TatI w'GTAC_w

Cuts at:      0    1007    2316 
   Size:       1007    1309
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     922     954    2316 
   Size:        922      32    1362

  Fragments arranged by size: 

                1362     922      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     912    2316 
   Size:        912    1404
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2040    2161    2316 
   Size:       2040     121     155

  Fragments arranged by size: 

                2040     155     121
 
XhoI C'TCGA_G

Cuts at:      0     370    2316 
   Size:        370    1946
 
XmnI GAAnn'nnTTC

Cuts at:      0      32    2316 
   Size:         32    2284
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI     ApaI     ApoI     AvaI    BamHI     BanI
    BanII     BbeI    BciVI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrGI
   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII     DrdI
     EaeI     EarI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I
     KasI     MmeI     MscI     MslI   MspA1I     NarI     NcoI     PasI
    PflMI     PfoI     PmlI    PpuMI    PshAI   PspOMI     PsrI     PstI
    PvuII     SapI     SfcI     SfoI     SmlI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI
     BmtI     BplI    BsaXI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI    BstBI   BstEII  BstZ17I
    BtgZI     ClaI    CspCI     DraI     EagI     EciI  EcoICRI    EcoRI
    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PmeI     PpiI     PsiI    PspXI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     XbaI     XmaI     ZraI
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