Restriction map for pF1KSDF0201
 (Linear) (Six-base) MAPSORT of: FLJ00201.seq  Check: 9204  from: 1  to: 2109



FLJ00201.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1643    1846    2109 
   Size:       1643     203     263

  Fragments arranged by size: 

                1643     263     203
 
AccI GT'mk_AC

Cuts at:      0     830    1856    2109 
   Size:        830    1026     253

  Fragments arranged by size: 

                1026     830     253
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244    1465    2109 
   Size:        244    1221     644

  Fragments arranged by size: 

                1221     644     244
 
AflIII A'CryG_T

Cuts at:      0    1895    2109 
   Size:       1895     214
 
AleI CACnn'nnGTG

Cuts at:      0    1735    1894    2109 
   Size:       1735     159     215

  Fragments arranged by size: 

                1735     215     159
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     683    2109 
   Size:        683    1426
 
AlwNI CAG_nnn'CTG

Cuts at:      0     189     802    1147    1825    2109 
   Size:        189     613     345     678     284

  Fragments arranged by size: 

                 678     613     345     284     189
 
ApaI G_GGCC'C

Cuts at:      0     347     496     548     575     593     654     741     811 
   Size:        347     149      52      27      18      61      87      70

Cuts at:    811     880     916     985    1057    1121    1248    1274    1427 
   Size:         69      36      69      72      64     127      26     153

Cuts at:   1427    1472    1514    1523    1558    1577    2109 
   Size:         45      42       9      35      19     532

  Fragments arranged by size: 

                 532     347     153     149     127      87      72      70
                  69      69      64      61      52      45      42      36
                  35      27      26      19      18       9
 
ApaLI G'TGCA_C

Cuts at:      0    1876    2109 
   Size:       1876     233
 
ApoI r'AATT_y

Cuts at:      0    1777    2109 
   Size:       1777     332
 
AvaI C'yCGr_G

Cuts at:      0     253     260     489     637     908    1570    1578    1761 
   Size:        253       7     229     148     271     662       8     183

Cuts at:   1761    2109 
   Size:        348

  Fragments arranged by size: 

                 662     348     271     253     229     183     148       8
                   7
 
BanI G'GyrC_C

Cuts at:      0     526     537     667    1591    1657    1888    2109 
   Size:        526      11     130     924      66     231     221

  Fragments arranged by size: 

                 924     526     231     221     130      66      11
 
BanII G_rGCy'C

Cuts at:      0     236     347     389     496     548     575     593     654 
   Size:        236     111      42     107      52      27      18      61

Cuts at:    654     741     811     880     916     985    1057    1121    1248 
   Size:         87      70      69      36      69      72      64     127

Cuts at:   1248    1274    1329    1355    1427    1472    1514    1523    1558 
   Size:         26      55      26      72      45      42       9      35

Cuts at:   1558    1577    2109 
   Size:         19     532

  Fragments arranged by size: 

                 532     236     127     111     107      87      72      72
                  70      69      69      64      61      55      52      45
                  42      42      36      35      27      26      26      19
                  18       9
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      17    2109 
   Size:         17    2092
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1690    1724    2109 
   Size:       1690      34     385

  Fragments arranged by size: 

                1690     385      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     100     281     411     491     500     683     938    1125 
   Size:        100     181     130      80       9     183     255     187

Cuts at:   1125    1439    1518    1572    1600    1723    1808    1847    2109 
   Size:        314      79      54      28     123      85      39     262

  Fragments arranged by size: 

                 314     262     255     187     183     181     130     123
                 100      85      80      79      54      39      28       9
            
 
Bme1580I G_kGCm'C

Cuts at:      0     347     496     531     548     575     593     654     672 
   Size:        347     149      35      17      27      18      61      18

Cuts at:    672     741     811     880     916     985    1057    1121    1248 
   Size:         69      70      69      36      69      72      64     127

Cuts at:   1248    1274    1427    1472    1514    1523    1558    1577    1596 
   Size:         26     153      45      42       9      35      19      19

Cuts at:   1596    1880    1891    2109 
   Size:        284      11     218

  Fragments arranged by size: 

                 347     284     218     153     149     127      72      70
                  69      69      69      64      61      45      42      36
                  35      35      27      26      19      19      18      18
                  17      11       9
 
BmgBI CAC'GTC

Cuts at:      0    1114    1881    2109 
   Size:       1114     767     228

  Fragments arranged by size: 

                1114     767     228
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     112    1277    2109 
   Size:        112    1165     832

  Fragments arranged by size: 

                1165     832     112
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     791     845    1169    1325    1384    1496    1978    2109 
   Size:        791      54     324     156      59     112     482     131

  Fragments arranged by size: 

                 791     482     324     156     131     112      59      54
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     705    2109 
   Size:        705    1404
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     595    2109 
   Size:        595    1514
 
BsaHI Gr'CG_yC

Cuts at:      0      76    1853    2109 
   Size:         76    1777     256

  Fragments arranged by size: 

                1777     256      76
 
BsaWI w'CCGG_w

Cuts at:      0    1784    2109 
   Size:       1784     325
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1542    1572    2059    2089    2109 
   Size:       1542      30     487      30      20

  Fragments arranged by size: 

                1542     487      30      30      20
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2067    2109 
   Size:       2067      42
 
BseYI C'CCAG_C

Cuts at:      0       3      57     340     984     988    1204    1507    1665 
   Size:          3      54     283     644       4     216     303     158

Cuts at:   1665    1695    1818    2109 
   Size:         30     123     291

  Fragments arranged by size: 

                 644     303     291     283     216     158     123      54
                  30       4       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2044    2109 
   Size:       2044      65
 
BsiHKAI G_wGCw'C

Cuts at:      0    1742    1880    1994    2109 
   Size:       1742     138     114     115

  Fragments arranged by size: 

                1742     138     115     114
 
BsmI GAATG_Cn'

Cuts at:      0    1172    2109 
   Size:       1172     937
 
Bsp1286I G_dGCh'C

Cuts at:      0     236     347     389     496     531     548     575     593 
   Size:        236     111      42     107      35      17      27      18

Cuts at:    593     654     672     741     811     880     916     985    1057 
   Size:         61      18      69      70      69      36      69      72

Cuts at:   1057    1121    1248    1274    1329    1355    1427    1472    1514 
   Size:         64     127      26      55      26      72      45      42

Cuts at:   1514    1523    1558    1577    1596    1742    1880    1891    1994 
   Size:          9      35      19      19     146     138      11     103

Cuts at:   1994    2109 
   Size:        115

  Fragments arranged by size: 

                 236     146     138     127     115     111     107     103
                  72      72      70      69      69      69      64      61
                  55      45      42      42      36      35      35      27
                  26      26      19      19      18      18      17      11
                   9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1643    1846    2109 
   Size:       1643     203     263

  Fragments arranged by size: 

                1643     263     203
 
BsrFI r'CCGG_y

Cuts at:      0      94    1722    1926    2109 
   Size:         94    1628     204     183

  Fragments arranged by size: 

                1628     204     183      94
 
BsrGI T'GTAC_A

Cuts at:      0    1910    2109 
   Size:       1910     199
 
BstEII G'GTnAC_C

Cuts at:      0    1180    2109 
   Size:       1180     929
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1978    2109 
   Size:       1978     131
 
Bsu36I CC'TnA_GG

Cuts at:      0     498    1130    1372    1383    2109 
   Size:        498     632     242      11     726

  Fragments arranged by size: 

                 726     632     498     242      11
 
BtgI C'CryG_G

Cuts at:      0      70     321    1248    1514    2060    2109 
   Size:         70     251     927     266     546      49

  Fragments arranged by size: 

                 927     546     266     251      70      49
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1605    2109 
   Size:       1605     504
 
BtsI GCAGTG_nn'

Cuts at:      0    1730    2109 
   Size:       1730     379
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      21    1403    2109 
   Size:         21    1382     706

  Fragments arranged by size: 

                1382     706      21
 
EaeI y'GGCC_r

Cuts at:      0    1800    1928    2109 
   Size:       1800     128     181

  Fragments arranged by size: 

                1800     181     128
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     791     845    1169    1325    1384    1465    1496 
   Size:        244     547      54     324     156      59      81      31

Cuts at:   1496    1978    2109 
   Size:        482     131

  Fragments arranged by size: 

                 547     482     324     244     156     131      81      59
                  54      31
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1969    2109 
   Size:       1969     140
 
EcoO109I rG'GnC_Cy

Cuts at:      0     148     265     344     456     466     475     493     544 
   Size:        148     117      79     112      10       9      18      51

Cuts at:    544     545     571     572     590     650     651     737     738 
   Size:          1      26       1      18      60       1      86       1

Cuts at:    738     781     817     876     905     912     913     931     964 
   Size:         43      36      59      29       7       1      18      33

Cuts at:    964     981     993    1053    1072    1118    1144    1153    1189 
   Size:         17      12      60      19      46      26       9      36

Cuts at:   1189    1218    1244    1378    1424    1468    1469    1519    1520 
   Size:         29      26     134      46      44       1      50       1

Cuts at:   1520    1530    1555    1564    1574    1582    1601    2109 
   Size:         10      25       9      10       8      19     508

  Fragments arranged by size: 

                 508     148     134     117     112      86      79      60
                  60      59      51      50      46      46      44      43
                  36      36      33      29      29      26      26      26
                  25      19      19      18      18      18      17      12
                  10      10      10       9       9       9       8       7
                   1       1       1       1       1       1       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1960    1992    2081    2109 
   Size:       1960      32      89      28

  Fragments arranged by size: 

                1960      89      32      28
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2015    2109 
   Size:       2015      94
 
MscI TGG'CCA

Cuts at:      0    1802    2109 
   Size:       1802     307
 
MslI CAynn'nnrTG

Cuts at:      0    1735    1771    1894    2109 
   Size:       1735      36     123     215

  Fragments arranged by size: 

                1735     215     123      36
 
MspA1I CmG'CkG

Cuts at:      0      72     207     700     988    1411    1808    1998    2109 
   Size:         72     135     493     288     423     397     190     111

  Fragments arranged by size: 

                 493     423     397     288     190     135     111      72
            
 
NaeI GCC'GGC

Cuts at:      0    1928    2109 
   Size:       1928     181
 
NcoI C'CATG_G

Cuts at:      0     321    1248    2109 
   Size:        321     927     861

  Fragments arranged by size: 

                 927     861     321
 
NgoMIV G'CCGG_C

Cuts at:      0    1926    2109 
   Size:       1926     183
 
NspI r_CATG'y

Cuts at:      0    1770    2109 
   Size:       1770     339
 
PasI CC'CwG_GG

Cuts at:      0     244     382     576     655     785     847     919    1123 
   Size:        244     138     194      79     130      62      72     204

Cuts at:   1123    1329    1357    1525    1597    2109 
   Size:        206      28     168      72     512

  Fragments arranged by size: 

                 512     244     206     204     194     168     138     130
                  79      72      72      62      28
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     189    1690    1825    1898    2109 
   Size:        189    1501     135      73     211

  Fragments arranged by size: 

                1501     211     189     135      73
 
PfoI T'CCnGG_A

Cuts at:      0     358     768     822    2109 
   Size:        358     410      54    1287

  Fragments arranged by size: 

                1287     410     358      54
 
PpuMI rG'GwC_Cy

Cuts at:      0     148     265     466     475     817     905     993    1072 
   Size:        148     117     201       9     342      88      88      79

Cuts at:   1072    1153    1218    1378    1530    1582    2109 
   Size:         81      65     160     152      52     527

  Fragments arranged by size: 

                 527     342     201     160     152     148     117      88
                  88      81      79      65      52       9
 
PspOMI G'GGCC_C

Cuts at:      0     343     492     544     571     589     650     737     807 
   Size:        343     149      52      27      18      61      87      70

Cuts at:    807     876     912     981    1053    1117    1244    1270    1423 
   Size:         69      36      69      72      64     127      26     153

Cuts at:   1423    1468    1510    1519    1554    1573    2109 
   Size:         45      42       9      35      19     536

  Fragments arranged by size: 

                 536     343     153     149     127      87      72      70
                  69      69      64      61      52      45      42      36
                  35      27      26      19      18       9
 
PstI C_TGCA'G

Cuts at:      0    1098    2109 
   Size:       1098    1011
 
PvuII CAG'CTG

Cuts at:      0     700     988    1411    1998    2109 
   Size:        700     288     423     587     111

  Fragments arranged by size: 

                 700     587     423     288     111
 
SacII CC_GC'GG

Cuts at:      0      73    2109 
   Size:         73    2036
 
SanDI GG'GwC_CC

Cuts at:      0     265     466     905     993    1072    1218    1378    1530 
   Size:        265     201     439      88      79     146     160     152

Cuts at:   1530    1582    2109 
   Size:         52     527

  Fragments arranged by size: 

                 527     439     265     201     160     152     146      88
                  79      52
 
SexAI A'CCwGG_T

Cuts at:      0     522     714    2012    2109 
   Size:        522     192    1298      97

  Fragments arranged by size: 

                1298     522     192      97
 
SfcI C'TryA_G

Cuts at:      0    1094    2109 
   Size:       1094    1015
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     491     683    1125    1439    1518    1572    2109 
   Size:        491     192     442     314      79      54     537

  Fragments arranged by size: 

                 537     491     442     314     192      79      54
 
SmaI CCC'GGG

Cuts at:      0     255     491     639     910    1572    1580    2109 
   Size:        255     236     148     271     662       8     529

  Fragments arranged by size: 

                 662     529     271     255     236     148       8
 
SmlI C'TyrA_G

Cuts at:      0     610    2109 
   Size:        610    1499
 
SphI G_CATG'C

Cuts at:      0    1770    2109 
   Size:       1770     339
 
SrfI GCCC'GGGC

Cuts at:      0     255     639    2109 
   Size:        255     384    1470

  Fragments arranged by size: 

                1470     384     255
 
StuI AGG'CCT

Cuts at:      0     608    1433    1460    2109 
   Size:        608     825      27     649

  Fragments arranged by size: 

                 825     649     608      27
 
StyI C'CwwG_G

Cuts at:      0     321     531     968    1248    1274    1428    2109 
   Size:        321     210     437     280      26     154     681

  Fragments arranged by size: 

                 681     437     321     280     210     154      26
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1890    2010    2109 
   Size:       1890     120      99

  Fragments arranged by size: 

                1890     120      99
 
TatI w'GTAC_w

Cuts at:      0     125    1910    1949    2066    2109 
   Size:        125    1785      39     117      43

  Fragments arranged by size: 

                1785     125     117      43      39
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1301    1333    1542    1574    2109 
   Size:       1301      32     209      32     535

  Fragments arranged by size: 

                1301     535     209      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2014    2109 
   Size:       2014      95
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     328     583    1192    1255    1408    1647    1899    1978 
   Size:        328     255     609      63     153     239     252      79

Cuts at:   1978    2109 
   Size:        131

  Fragments arranged by size: 

                 609     328     255     252     239     153     131      79
                  63
 
XmaI C'CCGG_G

Cuts at:      0     253     489     637     908    1570    1578    2109 
   Size:        253     236     148     271     662       8     531

  Fragments arranged by size: 

                 662     531     271     253     236     148       8
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AleI     AlfI    AlwNI     ApaI
    ApaLI     ApoI     AvaI     BanI    BanII     BbsI     BcgI     BglI
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI
    BsrFI    BsrGI   BstEII    BstXI   Bsu36I     BtgI    BtgZI     BtsI
     DrdI     EaeI  Eco57MI    EcoNI EcoO109I    Hin4I     MmeI     MscI
     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI     PasI    PflMI
     PfoI    PpuMI   PspOMI     PstI    PvuII    SacII    SanDI    SexAI
     SfcI     SfiI     SmaI     SmlI     SphI     SrfI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AloI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI
    BciVI     BclI    BfrBI    BglII     BlpI     BmtI     BplI     BsaI
    BsaAI    BsaBI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI   BssHII    BssSI   BstAPI    BstBI    BstYI  BstZ17I     ClaI
    CspCI     DraI   DraIII     EagI     EarI     EciI  EcoICRI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     NarI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI     SalI
     SapI     SbfI     ScaI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SspI     SwaI     XbaI     XhoI     XmnI     ZraI
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