Restriction map for pF1KSDF0168
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AocI        Aor51HI     
ApoI        AscI        AseI        AsnI        Asp718I     
AspI        AvrII       BbeI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BsaBI       
BsaWI       BseAI       BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrGI       
Bst1107I    BstBI       BstXI       BstZ17I     Bsu36I      
ClaI        DraI        Eco47III    Eco81I      EcoRI       
EcoRV       FbaI        HincII      HindII      HpaI        
KasI        KpnI        KspI        MaeI        MamI        
MfeI        MluI        MroI        MseI        MunI        
NarI        NcoI        NdeI        NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshBI       Psp1406I    RcaI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfoI        SfuI        SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tru9I       Tsp509I     Tth111I     XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AcyI         1315  GGCGTC               G[GA]^CG[CT]C
ApaI          743  GGGCCC               GGGCC^C
Asp700I       637  GAATATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI         1598  TGGCCA               TGG^CCA
BbsI          538  GAAGAC               GAAGAC(2/6)
BcgI         1310  GCACCGGCGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI         538  GAAGAC               GAAGAC(2/6)
BsaHI        1315  GGCGTC               G[GA]^CG[CT]C
BsaI          904  GAGACC               GGTCTC(1/5)
BspMI         800  ACCTGC               ACCTGC(4/8)
BsrBI         966  GAGCGG               CCGCTC(-3/-3)
BsrDI        1274  CATTGC               GCAATG(2/0)
BssSI        1686  CACGAG               CACGAG(-5/-1)
CpoI          969  CGGACCG              CG^G[AT]CCG
DrdI         1402  GACTGGACGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          857  CCGTGG               C^C[GA][CT]GG
EarI         1237  GAAGAG               CTCTTC(1/4)
EcoNI        1620  CCTGGCAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       105  ATGCAT               ATGCA^T
FseI          668  GGCCGGCC             GGCCGG^CC
Hin1I        1315  GGCGTC               G[GA]^CG[CT]C
HindIII      1071  AAGCTT               A^AGCTT
Ksp632I      1237  GAAGAG               CTCTTC(1/4)
MluNI        1598  TGGCCA               TGG^CCA
MscI         1598  TGGCCA               TGG^CCA
NsiI          105  ATGCAT               ATGCA^T
PflMI         384  CCAGGGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          767  GACTC                GAGTC(4/5)
PshAI        1473  GACGGCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          594  CTGCAG               CTGCA^G
PvuI         1128  CGATCG               CGAT^CG
RsrII         969  CGGACCG              CG^G[AT]CCG
SacI          822  GAGCTC               GAGCT^C
SfcI          594  CTGCAG               C^T[GA][CT]AG
TfiI          924  GAATC                G^A[AT]TC
TspRI         587  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I        384  CCAGGGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          637  GAATATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AflIII        431  ACACGT               A^C[GA][CT]GT
             1193  ACACGT              
AhdI          245  GACACAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              691  GACGTGATGTC         
Alw44I        445  GTGCAC               G^TGCAC
              889  GTGCAC              
AlwNI         385  CAGGGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              679  CAGCTTCTG           
ApaLI         445  GTGCAC               G^TGCAC
              889  GTGCAC              
AspEI         245  GACACAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              691  GACGTGATGTC         
AviII         344  TGCGCA               TGC^GCA
              461  TGCGCA              
BamHI         114  GGATCC               G^GATCC
              365  GGATCC              
BanI          483  GGTGCC               G^G[CT][GA]CC
             1309  GGCACC              
BbvCI          45  CCTCAGC              CCTCAGC(-5/-2)
              756  CCTCAGC             
BlpI          231  GCTCAGC              GC^T[ACGT]AGC
              819  GCTGAGC             
Bpu1102I      231  GCTCAGC              GC^T[ACGT]AGC
              819  GCTGAGC             
BsmBI         689  GAGACG               CGTCTC(1/5)
             1471  GAGACG              
BstAPI        882  GCACGATGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1041  GCACTACGTGC         
BstEII        144  GGTGACC              G^GT[ACGT]ACC
              252  GGTCACC             
BstPI         144  GGTGACC              G^GT[ACGT]ACC
              252  GGTCACC             
BstYI         114  GGATCC               [GA]^GATC[CT]
              365  GGATCC              
CelII         231  GCTCAGC              GC^T[ACGT]AGC
              819  GCTGAGC             
EagI          667  CGGCCG               C^GGCCG
              671  CGGCCG              
Eam1105I      245  GACACAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              691  GACGTGATGTC         
EclXI         667  CGGCCG               C^GGCCG
              671  CGGCCG              
Eco52I        667  CGGCCG               C^GGCCG
              671  CGGCCG              
EcoO65I       144  GGTGACC              G^GT[ACGT]ACC
              252  GGTCACC             
FspI          344  TGCGCA               TGC^GCA
              461  TGCGCA              
HinfI         767  GACTC                G^A[ACGT]TC
              924  GAATC               
MaeIII        145  GTGAC                ^GT[ACGT]AC
              253  GTCAC               
MflI          114  GGATCC               [GA]^GATC[CT]
              365  GGATCC              
PpuMI         797  GGGACCT              [GA]G^G[AT]CC[CT]
             1690  AGGACCT             
PvuII         130  CAGCTG               CAG^CTG
              750  CAGCTG              
SfiI            3  GGCCCCATTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
              735  GGCCCTCCGGGCC       
SgrAI        1311  CACCGGCG             C[GA]^CCGG[CT]G
             1373  CGCCGGCG            
SphI          107  GCATGC               GCATG^C
              347  GCATGC              
Tsp45I        145  GTGAC                ^GT[GC]AC
              253  GTCAC               
XhoII         114  GGATCC               [GA]^GATC[CT]
              365  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   87 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AspHI       AvaI        AvaII       BanII       BbrPI       
BbvI        BcnI        BglI        BmyI        BpmI        
BsaAI       BsaJI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrFI       BsrI        BssHII      
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HpaII       HphI        ItaI        MaeII       MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PmaCI       PmlI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SmlI        StyI        TaqI        
TseI        TthHB8I     
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