Restriction map for pF1KSDF0168
 (Linear) (Six-base) MAPSORT of: FLJ00168.seq  Check: 8262  from: 1  to: 1701



FLJ00168.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:19  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     949     958    1701 
   Size:        190     759       9     743

  Fragments arranged by size: 

                 759     743     190       9
 
AflIII A'CryG_T

Cuts at:      0     431    1193    1701 
   Size:        431     762     508

  Fragments arranged by size: 

                 762     508     431
 
AhdI GACnn_n'nnGTC

Cuts at:      0     250     696    1701 
   Size:        250     446    1005

  Fragments arranged by size: 

                1005     446     250
 
AleI CACnn'nnGTG

Cuts at:      0    1060    1701 
   Size:       1060     641
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     609    1701 
   Size:        609    1092
 
AlwNI CAG_nnn'CTG

Cuts at:      0     390     684    1701 
   Size:        390     294    1017

  Fragments arranged by size: 

                1017     390     294
 
ApaI G_GGCC'C

Cuts at:      0     747    1701 
   Size:        747     954
 
ApaLI G'TGCA_C

Cuts at:      0     445     889    1701 
   Size:        445     444     812

  Fragments arranged by size: 

                 812     445     444
 
AvaI C'yCGr_G

Cuts at:      0     487     646    1549    1701 
   Size:        487     159     903     152

  Fragments arranged by size: 

                 903     487     159     152
 
BamHI G'GATC_C

Cuts at:      0     114     365    1701 
   Size:        114     251    1336

  Fragments arranged by size: 

                1336     251     114
 
BanI G'GyrC_C

Cuts at:      0     483    1309    1701 
   Size:        483     826     392

  Fragments arranged by size: 

                 826     483     392
 
BanII G_rGCy'C

Cuts at:      0     158     398     747     826    1701 
   Size:        158     240     349      79     875

  Fragments arranged by size: 

                 875     349     240     158      79
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     545    1701 
   Size:        545    1156
 
BbvCI CC'TCA_GC

Cuts at:      0      46     757    1701 
   Size:         46     711     944

  Fragments arranged by size: 

                 944     711      46
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1299    1333    1701 
   Size:       1299      34     368

  Fragments arranged by size: 

                1299     368      34
 
BfrBI ATG'CAT

Cuts at:      0     107    1701 
   Size:        107    1594
 
BglI GCCn_nnn'nGGC

Cuts at:      0      10     742    1089    1355    1701 
   Size:         10     732     347     266     346

  Fragments arranged by size: 

                 732     347     346     266      10
 
BlpI GC'TnA_GC

Cuts at:      0     232     820    1701 
   Size:        232     588     881

  Fragments arranged by size: 

                 881     588     232
 
Bme1580I G_kGCm'C

Cuts at:      0     449     488     747     893    1559    1701 
   Size:        449      39     259     146     666     142

  Fragments arranged by size: 

                 666     449     259     146     142      39
 
BmgBI CAC'GTC

Cuts at:      0     693     948    1266    1701 
   Size:        693     255     318     435

  Fragments arranged by size: 

                 693     435     318     255
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     411     598    1701 
   Size:        411     187    1103

  Fragments arranged by size: 

                1103     411     187
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     754    1631    1701 
   Size:        283     471     877      70

  Fragments arranged by size: 

                 877     471     283      70
 
Bpu10I CC'TnA_GC

Cuts at:      0      46     661     757    1069    1701 
   Size:         46     615      96     312     632

  Fragments arranged by size: 

                 632     615     312      96      46
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     565    1248    1701 
   Size:        121     444     683     453

  Fragments arranged by size: 

                 683     453     444     121
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     898    1701 
   Size:        898     803
 
BsaAI yAC'GTr

Cuts at:      0     434     894    1047    1196    1701 
   Size:        434     460     153     149     505

  Fragments arranged by size: 

                 505     460     434     153     149
 
BsaHI Gr'CG_yC

Cuts at:      0    1316    1701 
   Size:       1316     385
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      13      34    1111    1701 
   Size:         13      21    1077     590

  Fragments arranged by size: 

                1077     590      21      13
 
BseYI C'CCAG_C

Cuts at:      0     333     352     495     794    1463    1701 
   Size:        333      19     143     299     669     238

  Fragments arranged by size: 

                 669     333     299     238     143      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       1     697     916    1024    1701 
   Size:          1     696     219     108     677

  Fragments arranged by size: 

                 696     677     219     108       1
 
BsiEI CG_ry'CG

Cuts at:      0     670     674    1131    1701 
   Size:        670       4     457     570

  Fragments arranged by size: 

                 670     570     457       4
 
BsiHKAI G_wGCw'C

Cuts at:      0     233     449     826     893    1103    1445    1701 
   Size:        233     216     377      67     210     342     256

  Fragments arranged by size: 

                 377     342     256     233     216     210      67
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     683    1465    1701 
   Size:        683     782     236

  Fragments arranged by size: 

                 782     683     236
 
Bsp1286I G_dGCh'C

Cuts at:      0     158     233     398     449     488     747     826     893 
   Size:        158      75     165      51      39     259      79      67

Cuts at:    893    1103    1445    1559    1701 
   Size:        210     342     114     142

  Fragments arranged by size: 

                 342     259     210     165     158     142     114      79
                  75      67      51      39
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     809    1701 
   Size:        809     892
 
BsrBI CCG'CTC

Cuts at:      0     968    1701 
   Size:        968     733
 
BsrDI GCAATG_nn'

Cuts at:      0    1273    1701 
   Size:       1273     428
 
BsrFI r'CCGG_y

Cuts at:      0     665     669     972    1050    1222    1312    1374    1435 
   Size:        665       4     303      78     172      90      62      61

Cuts at:   1435    1701 
   Size:        266

  Fragments arranged by size: 

                 665     303     266     172      90      78      62      61
                   4
 
BssHII G'CGCG_C

Cuts at:      0     810     812    1172    1494    1701 
   Size:        810       2     360     322     207

  Fragments arranged by size: 

                 810     360     322     207       2
 
BssSI C'ACGA_G

Cuts at:      0    1686    1701 
   Size:       1686      15
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     888    1047    1701 
   Size:        888     159     654

  Fragments arranged by size: 

                 888     654     159
 
BstEII G'GTnAC_C

Cuts at:      0     144     252    1701 
   Size:        144     108    1449

  Fragments arranged by size: 

                1449     144     108
 
BstYI r'GATC_y

Cuts at:      0     114     365    1701 
   Size:        114     251    1336

  Fragments arranged by size: 

                1336     251     114
 
BtgI C'CryG_G

Cuts at:      0     857    1701 
   Size:        857     844
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     251     619     880    1701 
   Size:        251     368     261     821

  Fragments arranged by size: 

                 821     368     261     251
 
DraIII CAC_nnn'GTG

Cuts at:      0     453     888    1047    1701 
   Size:        453     435     159     654

  Fragments arranged by size: 

                 654     453     435     159
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1408    1701 
   Size:       1408     293
 
EaeI y'GGCC_r

Cuts at:      0     206     218     667     671    1052    1113    1419    1541 
   Size:        206      12     449       4     381      61     306     122

Cuts at:   1541    1598    1701 
   Size:         57     103

  Fragments arranged by size: 

                 449     381     306     206     122     103      61      57
                  12       4
 
EagI C'GGCC_G

Cuts at:      0     667     671    1701 
   Size:        667       4    1030

  Fragments arranged by size: 

                1030     667       4
 
EarI CTCTTCn'nnn_

Cuts at:      0    1232    1701 
   Size:       1232     469
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1348    1701 
   Size:       1348     353
 
EcoICRI GAG'CTC

Cuts at:      0     824    1701 
   Size:        824     877
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     283     754     949     958    1631    1701 
   Size:        190      93     471     195       9     673      70

  Fragments arranged by size: 

                 673     471     195     190      93      70       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1624    1701 
   Size:       1624      77
 
EcoO109I rG'GnC_Cy

Cuts at:      0     387     567     735     798    1082    1525    1691    1701 
   Size:        387     180     168      63     284     443     166      10

  Fragments arranged by size: 

                 443     387     284     180     168     166      63      10
            
 
FseI GG_CCGG'CC

Cuts at:      0     673    1701 
   Size:        673    1028
 
FspI TGC'GCA

Cuts at:      0     346     463    1701 
   Size:        346     117    1238

  Fragments arranged by size: 

                1238     346     117
 
FspAI rTGC'GCAy

Cuts at:      0     346    1701 
   Size:        346    1355
 
HaeII r_GCGC'y

Cuts at:      0     200    1030    1258    1381    1492    1701 
   Size:        200     830     228     123     111     209

  Fragments arranged by size: 

                 830     228     209     200     123     111
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     236     268     682     714    1120    1152    1701 
   Size:        236      32     414      32     406      32     549

  Fragments arranged by size: 

                 549     414     406     236      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1071    1701 
   Size:       1071     630
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1181    1701 
   Size:       1181     520
 
MscI TGG'CCA

Cuts at:      0    1600    1701 
   Size:       1600     101
 
MslI CAynn'nnrTG

Cuts at:      0     346    1060    1589    1701 
   Size:        346     714     529     112

  Fragments arranged by size: 

                 714     529     346     112
 
MspA1I CmG'CkG

Cuts at:      0     132     752    1005    1701 
   Size:        132     620     253     696

  Fragments arranged by size: 

                 696     620     253     132
 
NaeI GCC'GGC

Cuts at:      0     667     671    1052    1224    1376    1437    1701 
   Size:        667       4     381     172     152      61     264

  Fragments arranged by size: 

                 667     381     264     172     152      61       4
 
NgoMIV G'CCGG_C

Cuts at:      0     665     669    1050    1222    1374    1435    1701 
   Size:        665       4     381     172     152      61     266

  Fragments arranged by size: 

                 665     381     266     172     152      61       4
 
NsiI A_TGCA'T

Cuts at:      0     109    1701 
   Size:        109    1592
 
NspI r_CATG'y

Cuts at:      0     111     351    1020    1588    1701 
   Size:        111     240     669     568     113

  Fragments arranged by size: 

                 669     568     240     113     111
 
PasI CC'CwG_GG

Cuts at:      0     873    1701 
   Size:        873     828
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     390    1701 
   Size:        390    1311
 
PfoI T'CCnGG_A

Cuts at:      0     260    1701 
   Size:        260    1441
 
PmlI CAC'GTG

Cuts at:      0     434     894    1196    1701 
   Size:        434     460     302     505

  Fragments arranged by size: 

                 505     460     434     302
 
PpuMI rG'GwC_Cy

Cuts at:      0     798    1691    1701 
   Size:        798     893      10

  Fragments arranged by size: 

                 893     798      10
 
PshAI GACnn'nnGTC

Cuts at:      0    1477    1701 
   Size:       1477     224
 
PspOMI G'GGCC_C

Cuts at:      0     743    1701 
   Size:        743     958
 
PstI C_TGCA'G

Cuts at:      0     598    1701 
   Size:        598    1103
 
PvuI CG_AT'CG

Cuts at:      0    1131    1701 
   Size:       1131     570
 
PvuII CAG'CTG

Cuts at:      0     132     752    1701 
   Size:        132     620     949

  Fragments arranged by size: 

                 949     620     132
 
RsrII CG'GwC_CG

Cuts at:      0     970    1701 
   Size:        970     731
 
SacI G_AGCT'C

Cuts at:      0     826    1701 
   Size:        826     875
 
SfcI C'TryA_G

Cuts at:      0     594    1701 
   Size:        594    1107
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      10     742    1701 
   Size:         10     732     959

  Fragments arranged by size: 

                 959     732      10
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1312    1374    1701 
   Size:       1312      62     327

  Fragments arranged by size: 

                1312     327      62
 
SmlI C'TyrA_G

Cuts at:      0     136     580    1227    1701 
   Size:        136     444     647     474

  Fragments arranged by size: 

                 647     474     444     136
 
SphI G_CATG'C

Cuts at:      0     111     351    1701 
   Size:        111     240    1350

  Fragments arranged by size: 

                1350     240     111
 
StyI C'CwwG_G

Cuts at:      0     224    1058    1653    1701 
   Size:        224     834     595      48

  Fragments arranged by size: 

                 834     595     224      48
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     430     778    1455    1701 
   Size:        430     348     677     246

  Fragments arranged by size: 

                 677     430     348     246
 
TatI w'GTAC_w

Cuts at:      0     773    1701 
   Size:        773     928
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     962     994    1701 
   Size:        962      32     707

  Fragments arranged by size: 

                 962     707      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     641    1701 
   Size:        641    1060
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI   BstEII    BstYI     BtgI    BtgZI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FseI
     FspI    FspAI    HaeII    Hin4I  HindIII     MmeI     MscI     MslI
   MspA1I     NaeI   NgoMIV     NsiI     NspI     PasI    PflMI     PfoI
     PmlI    PpuMI    PshAI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI     SfcI     SfiI    SgrAI     SmlI     SphI     StyI    TaqII
     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AloI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
    BciVI     BclI    BglII     BmtI     BplI    BsaBI    BsaWI    BsaXI
    BsiWI     BsmI    BspEI    BspHI    BsrGI    BstBI    BstXI  BstZ17I
   Bsu36I     BtsI     ClaI    CspCI     DraI    EcoRI    EcoRV     FalI
   HincII     HpaI     KasI     KpnI     MfeI     MluI     NarI     NcoI
     NdeI     NheI     NotI     NruI     PacI     PciI     PmeI     PpiI
     PsiI    PspXI     PsrI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfoI     SgfI     SmaI    SnaBI     SpeI     SrfI
     SspI     StuI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com