Restriction map for pF1KSDF0166
 (Linear) (Six-base) MAPSORT of: FLJ00166.seq  Check: 9665  from: 1  to: 1317



FLJ00166.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     261    1278    1317 
   Size:        261    1017      39

  Fragments arranged by size: 

                1017     261      39
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     698     890    1317 
   Size:        698     192     427

  Fragments arranged by size: 

                 698     427     192
 
AflII C'TTAA_G

Cuts at:      0     498    1317 
   Size:        498     819
 
ApaI G_GGCC'C

Cuts at:      0     133    1317 
   Size:        133    1184
 
ApoI r'AATT_y

Cuts at:      0     196     412     790     861    1317 
   Size:        196     216     378      71     456

  Fragments arranged by size: 

                 456     378     216     196      71
 
AseI AT'TA_AT

Cuts at:      0     269    1317 
   Size:        269    1048
 
AvaI C'yCGr_G

Cuts at:      0     125    1317 
   Size:        125    1192
 
BamHI G'GATC_C

Cuts at:      0     531    1317 
   Size:        531     786
 
BanII G_rGCy'C

Cuts at:      0     133    1317 
   Size:        133    1184
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     281    1317 
   Size:        281    1036
 
Bme1580I G_kGCm'C

Cuts at:      0     133    1317 
   Size:        133    1184
 
BmtI G_CTAG'C

Cuts at:      0     610    1317 
   Size:        610     707
 
BsaHI Gr'CG_yC

Cuts at:      0     963    1317 
   Size:        963     354
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1178    1317 
   Size:       1178     139
 
BseYI C'CCAG_C

Cuts at:      0     140    1317 
   Size:        140    1177
 
BsiEI CG_ry'CG

Cuts at:      0     113    1317 
   Size:        113    1204
 
Bsp1286I G_dGCh'C

Cuts at:      0     133    1317 
   Size:        133    1184
 
BspHI T'CATG_A

Cuts at:      0     455    1317 
   Size:        455     862
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     261    1278    1317 
   Size:        261    1017      39

  Fragments arranged by size: 

                1017     261      39
 
BssSI C'ACGA_G

Cuts at:      0    1037    1317 
   Size:       1037     280
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     150    1051    1258    1317 
   Size:        150     901     207      59

  Fragments arranged by size: 

                 901     207     150      59
 
BstYI r'GATC_y

Cuts at:      0     216     531    1317 
   Size:        216     315     786

  Fragments arranged by size: 

                 786     315     216
 
BtgI C'CryG_G

Cuts at:      0      36     525    1317 
   Size:         36     489     792

  Fragments arranged by size: 

                 792     489      36
 
DraI TTT'AAA

Cuts at:      0     923     954    1196    1245    1317 
   Size:        923      31     242      49      72

  Fragments arranged by size: 

                 923     242      72      49      31
 
EaeI y'GGCC_r

Cuts at:      0     110    1317 
   Size:        110    1207
 
EagI C'GGCC_G

Cuts at:      0     110    1317 
   Size:        110    1207
 
EarI CTCTTCn'nnn_

Cuts at:      0     163     633     638     674    1317 
   Size:        163     470       5      36     643

  Fragments arranged by size: 

                 643     470     163      36       5
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     698     890    1317 
   Size:        698     192     427

  Fragments arranged by size: 

                 698     427     192
 
EcoO109I rG'GnC_Cy

Cuts at:      0       9     129    1317 
   Size:          9     120    1188

  Fragments arranged by size: 

                1188     120       9
 
EcoRI G'AATT_C

Cuts at:      0     412     790    1317 
   Size:        412     378     527

  Fragments arranged by size: 

                 527     412     378
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     523     555     560     592    1317 
   Size:        523      32       5      32     725

  Fragments arranged by size: 

                 725     523      32      32       5
 
HincII GTy'rAC

Cuts at:      0     177     945    1317 
   Size:        177     768     372

  Fragments arranged by size: 

                 768     372     177
 
HindIII A'AGCT_T

Cuts at:      0     995    1025    1220    1317 
   Size:        995      30     195      97

  Fragments arranged by size: 

                 995     195      97      30
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     939    1138    1317 
   Size:        939     199     179

  Fragments arranged by size: 

                 939     199     179
 
MslI CAynn'nnrTG

Cuts at:      0     971    1124    1317 
   Size:        971     153     193

  Fragments arranged by size: 

                 971     193     153
 
NcoI C'CATG_G

Cuts at:      0     525    1317 
   Size:        525     792
 
NheI G'CTAG_C

Cuts at:      0     606    1317 
   Size:        606     711
 
PfoI T'CCnGG_A

Cuts at:      0     235     777    1317 
   Size:        235     542     540

  Fragments arranged by size: 

                 542     540     235
 
PpuMI rG'GwC_Cy

Cuts at:      0       9    1317 
   Size:          9    1308
 
PspOMI G'GGCC_C

Cuts at:      0     129    1317 
   Size:        129    1188
 
SapI GCTCTTCn'nnn_

Cuts at:      0     633     674    1317 
   Size:        633      41     643

  Fragments arranged by size: 

                 643     633      41
 
SfcI C'TryA_G

Cuts at:      0     696    1317 
   Size:        696     621
 
SmaI CCC'GGG

Cuts at:      0     127    1317 
   Size:        127    1190
 
SmlI C'TyrA_G

Cuts at:      0     498    1317 
   Size:        498     819
 
StuI AGG'CCT

Cuts at:      0     848    1317 
   Size:        848     469
 
StyI C'CwwG_G

Cuts at:      0     525     761    1317 
   Size:        525     236     556

  Fragments arranged by size: 

                 556     525     236
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     953     985    1317 
   Size:        953      32     332

  Fragments arranged by size: 

                 953     332      32
 
XbaI T'CTAG_A

Cuts at:      0     798    1317 
   Size:        798     519
 
XmaI C'CCGG_G

Cuts at:      0     125    1317 
   Size:        125    1192
 
XmnI GAAnn'nnTTC

Cuts at:      0     399     683     794    1317 
   Size:        399     284     111     523

  Fragments arranged by size: 

                 523     399     284     111
 

 Enzymes that do cut:

     AarI     AcuI    AflII     ApaI     ApoI     AseI     AvaI    BamHI
    BanII     BbsI Bme1580I     BmtI    BsaHI    BseRI    BseYI    BsiEI
 Bsp1286I    BspHI    BspMI    BssSI   BstAPI    BstYI     BtgI     DraI
     EaeI     EagI     EarI  Eco57MI EcoO109I    EcoRI    Hin4I   HincII
  HindIII     MmeI     MslI     NcoI     NheI     PfoI    PpuMI   PspOMI
     SapI     SfcI     SmaI     SmlI     StuI     StyI     TstI     XbaI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI    ApaLI     AscI    AsiSI    AvrII
     BaeI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI     BsgI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EciI  EcoICRI    EcoNI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI
     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TatI  Tth111I     XcmI
     XhoI     ZraI
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