Restriction map for pF1KSDF0106
##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflIII      Alw44I      AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
AspHI       AspI        AvrII       BamHI       BbrPI       
BciVI       BclI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BseAI       BsiHKAI     
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraIII      DsaI        
EarI        Eco47III    Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        HpaI        Ksp632I     
KspI        MamI        MfeI        MluI        MroI        
MunI        NcoI        NdeI        NheI        NotI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        TfiI        Tsp509I     Tth111I     
TthHB8I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I        911  GGTACC               G^GTACC
AcyI         1045  GGCGCC               G[GA]^CG[CT]C
AflII         351  CTTAAG               C^TTAAG
AgeI          242  ACCGGT               A^CCGGT
AhdI          502  GACCGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          204  GATCC                GGATC(4/5)
AlwNI         466  CAGAGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I       911  GGTACC               G^GTACC
AspEI         502  GACCGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         893  TGCGCA               TGC^GCA
BbeI         1045  GGCGCC               GGCGC^C
BcgI          678  GCAGGAAAATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          422  CTAG                 C^TAG
BfrI          351  CTTAAG               C^TTAAG
BsaHI        1045  GGCGCC               G[GA]^CG[CT]C
BsaWI         242  ACCGGT               [AT]^CCGG[AT]
BsgI          864  GTGCAG               GTGCAG(16/14)
BsiEI         549  CGGCCG               CG[GA][CT]^CG
BsmBI         257  CGTCTC               CGTCTC(1/5)
BsrBI         100  GAGCGG               CCGCTC(-3/-3)
BstYI         203  AGATCC               [GA]^GATC[CT]
DpnII         204  GATC                 ^GATC
DrdI          655  GACTCGGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          549  CGGCCG               C^GGCCG
Eam1105I      502  GACCGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         549  CGGCCG               C^GGCCG
Eco52I        549  CGGCCG               C^GGCCG
FseI          540  GGCCGGCC             GGCCGG^CC
FspI          893  TGCGCA               TGC^GCA
Hin1I        1045  GGCGCC               G[GA]^CG[CT]C
HincII          6  GTTGAC               GT[CT]^[GA]AC
HindII          6  GTTGAC               GT[CT]^[GA]AC
HindIII      1022  AAGCTT               A^AGCTT
KasI         1045  GGCGCC               G^GCGCC
KpnI          911  GGTACC               GGTAC^C
MaeI          422  CTAG                 C^TAG
MaeII         256  ACGT                 A^CGT
MboI          204  GATC                 ^GATC
MflI          203  AGATCC               [GA]^GATC[CT]
MseI          352  TTAA                 T^TAA
NarI         1045  GGCGCC               GG^CGCC
NdeII         204  GATC                 ^GATC
PflMI         465  CCAGAGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         242  ACCGGT               A^CCGGT
PpuMI         355  AGGTCCT              [GA]G^G[AT]CC[CT]
PvuII         235  CAGCTG               CAG^CTG
Sau3AI        204  GATC                 ^GATC
SfoI         1045  GGCGCC               GGC^GCC
SmlI          351  CTTAAG               C^T[CT][GA]AG
Tru9I         352  TTAA                 T^TAA
Van91I        465  CCAGAGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          608  CCAGGCCCAGCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         203  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AfaI          869  GTAC                 GT^AC
              912  GTAC                
ApaI          594  GGGCCC               GGGCC^C
              824  GGGCCC              
AvaII         240  GGACC                G^G[AT]CC
              356  GGTCC               
BalI          149  TGGCCA               TGG^CCA
              830  TGGCCA              
BanI          911  GGTACC               G^G[CT][GA]CC
             1045  GGCGCC              
BbsI         1068  GAAGAC               GAAGAC(2/6)
              664  GTCTTC              
BbvCI         381  CCTCAGC              CCTCAGC(-5/-2)
              751  GCTGAGG             
BpuAI        1068  GAAGAC               GAAGAC(2/6)
              664  GTCTTC              
BsaI         1077  GAGACC               GGTCTC(1/5)
              245  GGTCTC              
BseRI         640  CTCCTC               GAGGAG(10/8)
              754  GAGGAG              
BstXI          28  CCAGCGCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              821  CCAGGGCCCTGG        
EcoNI         347  CCTCCTTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              645  CCTGGACGAGG         
EcoT14I       113  CCAAGG               C^C[AT][AT]GG
              287  CCTTGG              
HaeII          30  AGCGCC               [GA]GCGC^[CT]
             1045  GGCGCC              
HgaI          118  GACGC                GACGC(5/10)
              985  GACGC               
HphI           84  GGTGA                GGTGA(8/7)
              409  TCACC               
MaeIII         85  GTGAC                ^GT[ACGT]AC
             1014  GTCAC               
MluNI         149  TGGCCA               TGG^CCA
              830  TGGCCA              
MscI          149  TGGCCA               TGG^CCA
              830  TGGCCA              
MspA1I        235  CAGCTG               C[AC]G^C[GT]G
              494  CCGCTG              
NaeI          394  GCCGGC               GCC^GGC
              541  GCCGGC              
NgoMIV        394  GCCGGC               G^CCGGC
              541  GCCGGC              
NruI          687  TCGCGA               TCG^CGA
              878  TCGCGA              
PstI          232  CTGCAG               CTGCA^G
              676  CTGCAG              
RsaI          869  GTAC                 GT^AC
              912  GTAC                
SfcI          232  CTGCAG               C^T[GA][CT]AG
              676  CTGCAG              
SmaI          483  CCCGGG               CCC^GGG
              591  CCCGGG              
SphI          176  GCATGC               GCATG^C
              183  GCATGC              
StyI          113  CCAAGG               C^C[AT][AT]GG
              287  CCTTGG              
Tsp45I         85  GTGAC                ^GT[GC]AC
             1014  GTCAC               
XmaCI         483  CCCGGG               C^CCGGG
              591  CCCGGG              
XmaI          483  CCCGGG               C^CCGGG
              591  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AccII       AciI        AluI        AvaI        BanII       
BbvI        BcnI        BglI        BmyI        BpmI        
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DraII       EaeI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       ItaI        MboII       MnlI        MslI        
MspI        MvaI        MvnI        MwoI        NciI        
NlaIII      NlaIV       NspI        PleI        Sau96I      
ScrFI       SfaNI       TseI        TspRI       
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