Restriction map for pF1KSDF0106
 (Linear) (Six-base) MAPSORT of: FLJ00106.seq  Check: 66  from: 1  to: 1098



FLJ00106.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:19  ..

Acc65I G'GTAC_C

Cuts at:      0     911    1098 
   Size:        911     187
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     979    1098 
   Size:        979     119
 
AflII C'TTAA_G

Cuts at:      0     351    1098 
   Size:        351     747
 
AgeI A'CCGG_T

Cuts at:      0     242    1098 
   Size:        242     856
 
AhdI GACnn_n'nnGTC

Cuts at:      0     507    1098 
   Size:        507     591
 
AleI CACnn'nnGTG

Cuts at:      0     854    1098 
   Size:        854     244
 
AlwNI CAG_nnn'CTG

Cuts at:      0     471    1098 
   Size:        471     627
 
ApaI G_GGCC'C

Cuts at:      0     598     828    1098 
   Size:        598     230     270

  Fragments arranged by size: 

                 598     270     230
 
AvaI C'yCGr_G

Cuts at:      0     387     483     591    1098 
   Size:        387      96     108     507

  Fragments arranged by size: 

                 507     387     108      96
 
BanI G'GyrC_C

Cuts at:      0     911    1045    1098 
   Size:        911     134      53

  Fragments arranged by size: 

                 911     134      53
 
BanII G_rGCy'C

Cuts at:      0     217     319     598     799     828    1098 
   Size:        217     102     279     201      29     270

  Fragments arranged by size: 

                 279     270     217     201     102      29
 
BbeI G_GCGC'C

Cuts at:      0    1049    1098 
   Size:       1049      49
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     657    1075    1098 
   Size:        657     418      23

  Fragments arranged by size: 

                 657     418      23
 
BbvCI CC'TCA_GC

Cuts at:      0     382     752    1098 
   Size:        382     370     346

  Fragments arranged by size: 

                 382     370     346
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     667     701    1098 
   Size:        667      34     397

  Fragments arranged by size: 

                 667     397      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     228     557     748     823     923    1098 
   Size:        228     329     191      75     100     175

  Fragments arranged by size: 

                 329     228     191     175     100      75
 
Bme1580I G_kGCm'C

Cuts at:      0     598     828     974    1098 
   Size:        598     230     146     124

  Fragments arranged by size: 

                 598     230     146     124
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     795    1098 
   Size:        795     303
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     155     245     351     458     591     766     804    1098 
   Size:        155      90     106     107     133     175      38     294

  Fragments arranged by size: 

                 294     175     155     133     107     106      90      38
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     382     752    1098 
   Size:        382     370     346

  Fragments arranged by size: 

                 382     370     346
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     251    1071    1098 
   Size:        251     820      27

  Fragments arranged by size: 

                 820     251      27
 
BsaHI Gr'CG_yC

Cuts at:      0    1046    1098 
   Size:       1046      52
 
BsaWI w'CCGG_w

Cuts at:      0     242    1098 
   Size:        242     856
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     895     925    1098 
   Size:        895      30     173

  Fragments arranged by size: 

                 895     173      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     631     769    1098 
   Size:        631     138     329

  Fragments arranged by size: 

                 631     329     138
 
BseYI C'CCAG_C

Cuts at:      0     613     784    1098 
   Size:        613     171     314

  Fragments arranged by size: 

                 613     314     171
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     885    1098 
   Size:        885     213
 
BsiEI CG_ry'CG

Cuts at:      0     552    1098 
   Size:        552     546
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     263    1098 
   Size:        263     835
 
Bsp1286I G_dGCh'C

Cuts at:      0     217     319     598     799     828     974    1098 
   Size:        217     102     279     201      29     146     124

  Fragments arranged by size: 

                 279     217     201     146     124     102      29
 
BsrBI CCG'CTC

Cuts at:      0     102    1098 
   Size:        102     996
 
BsrFI r'CCGG_y

Cuts at:      0     242     394     541     908    1098 
   Size:        242     152     147     367     190

  Fragments arranged by size: 

                 367     242     190     152     147
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      35     828    1098 
   Size:         35     793     270

  Fragments arranged by size: 

                 793     270      35
 
BstYI r'GATC_y

Cuts at:      0     203    1098 
   Size:        203     895
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     861    1098 
   Size:        861     237
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     661    1098 
   Size:        661     437
 
EaeI y'GGCC_r

Cuts at:      0      20      95     149     446     549     830    1098 
   Size:         20      75      54     297     103     281     268

  Fragments arranged by size: 

                 297     281     268     103      75      54      20
 
EagI C'GGCC_G

Cuts at:      0     549    1098 
   Size:        549     549
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     196    1098 
   Size:        196     902
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     155     245     351     458     591     766     804     979 
   Size:        155      90     106     107     133     175      38     175

Cuts at:    979    1098 
   Size:        119

  Fragments arranged by size: 

                 175     175     155     133     119     107     106      90
                  38
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     351     649    1098 
   Size:        351     298     449

  Fragments arranged by size: 

                 449     351     298
 
EcoO109I rG'GnC_Cy

Cuts at:      0     356     595     824     825    1098 
   Size:        356     239     229       1     273

  Fragments arranged by size: 

                 356     273     239     229       1
 
FseI GG_CCGG'CC

Cuts at:      0     545    1098 
   Size:        545     553
 
FspI TGC'GCA

Cuts at:      0     895    1098 
   Size:        895     203
 
FspAI rTGC'GCAy

Cuts at:      0     895    1098 
   Size:        895     203
 
HaeII r_GCGC'y

Cuts at:      0      34    1049    1098 
   Size:         34    1015      49

  Fragments arranged by size: 

                1015      49      34
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     128     160     328     360     493     525     640     672 
   Size:        128      32     168      32     133      32     115      32

Cuts at:    672    1098 
   Size:        426

  Fragments arranged by size: 

                 426     168     133     128     115      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0       8    1098 
   Size:          8    1090
 
HindIII A'AGCT_T

Cuts at:      0    1022    1098 
   Size:       1022      76
 
KasI G'GCGC_C

Cuts at:      0    1045    1098 
   Size:       1045      53
 
KpnI G_GTAC'C

Cuts at:      0     915    1098 
   Size:        915     183
 
MscI TGG'CCA

Cuts at:      0     151     832    1098 
   Size:        151     681     266

  Fragments arranged by size: 

                 681     266     151
 
MslI CAynn'nnrTG

Cuts at:      0      82     145     854    1098 
   Size:         82      63     709     244

  Fragments arranged by size: 

                 709     244      82      63
 
MspA1I CmG'CkG

Cuts at:      0     237     496    1098 
   Size:        237     259     602

  Fragments arranged by size: 

                 602     259     237
 
NaeI GCC'GGC

Cuts at:      0     396     543    1098 
   Size:        396     147     555

  Fragments arranged by size: 

                 555     396     147
 
NarI GG'CG_CC

Cuts at:      0    1046    1098 
   Size:       1046      52
 
NgoMIV G'CCGG_C

Cuts at:      0     394     541    1098 
   Size:        394     147     557

  Fragments arranged by size: 

                 557     394     147
 
NruI TCG'CGA

Cuts at:      0     689     880    1098 
   Size:        689     191     218

  Fragments arranged by size: 

                 689     218     191
 
NspI r_CATG'y

Cuts at:      0     180     187     479    1098 
   Size:        180       7     292     619

  Fragments arranged by size: 

                 619     292     180       7
 
PasI CC'CwG_GG

Cuts at:      0      35    1098 
   Size:         35    1063
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     471    1098 
   Size:        471     627
 
PfoI T'CCnGG_A

Cuts at:      0     644     899    1098 
   Size:        644     255     199

  Fragments arranged by size: 

                 644     255     199
 
PpuMI rG'GwC_Cy

Cuts at:      0     356    1098 
   Size:        356     742
 
PspOMI G'GGCC_C

Cuts at:      0     594     824    1098 
   Size:        594     230     274

  Fragments arranged by size: 

                 594     274     230
 
PstI C_TGCA'G

Cuts at:      0     236     680    1098 
   Size:        236     444     418

  Fragments arranged by size: 

                 444     418     236
 
PvuII CAG'CTG

Cuts at:      0     237    1098 
   Size:        237     861
 
SfcI C'TryA_G

Cuts at:      0     232     676    1098 
   Size:        232     444     422

  Fragments arranged by size: 

                 444     422     232
 
SfoI GGC'GCC

Cuts at:      0    1047    1098 
   Size:       1047      51
 
SmaI CCC'GGG

Cuts at:      0     485     593    1098 
   Size:        485     108     505

  Fragments arranged by size: 

                 505     485     108
 
SmlI C'TyrA_G

Cuts at:      0     351    1098 
   Size:        351     747
 
SphI G_CATG'C

Cuts at:      0     180     187    1098 
   Size:        180       7     911

  Fragments arranged by size: 

                 911     180       7
 
StyI C'CwwG_G

Cuts at:      0     113     287    1098 
   Size:        113     174     811

  Fragments arranged by size: 

                 811     174     113
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     615    1098 
   Size:        615     483
 
XmaI C'CCGG_G

Cuts at:      0     483     591    1098 
   Size:        483     108     507

  Fragments arranged by size: 

                 507     483     108
 

 Enzymes that do cut:

   Acc65I     AcuI    AflII     AgeI     AhdI     AleI    AlwNI     ApaI
     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BglI
 Bme1580I     BmrI     BpmI   Bpu10I     BsaI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI    BsmBI Bsp1286I    BsrBI    BsrFI
    BstXI    BstYI    BtgZI     DrdI     EaeI     EagI     EciI  Eco57MI
    EcoNI EcoO109I     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NruI     NspI     PasI    PflMI     PfoI    PpuMI   PspOMI
     PstI    PvuII     SfcI     SfoI     SmaI     SmlI     SphI     StyI
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI   AflIII     AlfI     AloI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI
    BpuEI    BsaAI    BsaBI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI
    BspMI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI   DraIII
     EarI  EcoICRI    EcoRI    EcoRV     FalI     HpaI     MfeI     MluI
     MmeI     NcoI     NdeI     NheI     NotI     NsiI     PacI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmnI     ZraI
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