Restriction map for pF1KSDF0041
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AccII       AccIII      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     AspEI       BalI        BbeI        
BbrPI       BbvCI       BcgI        BclI        BfrI        
BglII       BsaAI       BsaBI       BsaI        BseAI       
BsiEI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrGI       BsrI        BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        HincII      HindII      HindIII     HpaI        
KasI        KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MunI        
MvnI        NarI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        ScaI        SexAI       
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        SspBI       SspI        StuI        
SwaI        Tru9I       Tsp509I     TspRI       XbaI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII         877  GACGTC               GACGT^C
Acc65I        215  GGTACC               G^GTACC
AccI          643  GTCTAC               GT^[AC][GT]AC
AlwNI         682  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          171  GGGCCC               GGGCC^C
Asp718I       215  GGTACC               G^GTACC
AspHI         469  GAGCAC               G[AT]GC[AT]^C
AspI          898  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         398  TGCGCA               TGC^GCA
AvrII          78  CCTAGG               C^CTAGG
BamHI          43  GGATCC               G^GATCC
BbsI          880  GTCTTC               GAAGAC(2/6)
BciVI         758  GGATAC               GTATCC(6/5)
BfaI           79  CTAG                 C^TAG
BglI           16  GCCCTGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI           78  CCTAGG               C^CTAGG
BlpI          340  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      340  GCTCAGC              GC^T[ACGT]AGC
BpuAI         880  GTCTTC               GAAGAC(2/6)
BsaWI          84  TCCGGT               [AT]^CCGG[AT]
BsiHKAI       469  GAGCAC               G[AT]GC[AT]^C
BsmBI         875  GAGACG               CGTCTC(1/5)
BstYI          43  GGATCC               [GA]^GATC[CT]
CelII         340  GCTCAGC              GC^T[ACGT]AGC
DraIII        190  CACGATGTG            CAC[ACGT][ACGT][ACGT]^GTG
FspI          398  TGCGCA               TGC^GCA
KpnI          215  GGTACC               GGTAC^C
MaeI           79  CTAG                 C^TAG
MflI           43  GGATCC               [GA]^GATC[CT]
MslI          390  CATCACCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I        225  CTCGAG               C^TCGAG
PflFI         898  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         284  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII          30  CAGCTG               CAG^CTG
SapI           51  GCTCTTC              GCTCTTC(1/4)
SbfI          158  CCTGCAGG             CCTGCA^GG
SfiI           15  GGCCCTGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI          225  CTCGAG               C^T[CT][GA]AG
Sse8387I      158  CCTGCAGG             CCTGCA^GG
TaqI          226  TCGA                 T^CGA
TfiI          611  GAATC                G^A[AT]TC
Tth111I       898  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       226  TCGA                 T^CGA
XcmI          326  CCAGCCACGACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          225  CTCGAG               C^TCGAG
XhoII          43  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcyI            4  GGCGTC               G[GA]^CG[CT]C
              877  GACGTC              
AfaI          216  GTAC                 GT^AC
              540  GTAC                
AlwI           44  GATCC                GGATC(4/5)
               43  GGATC               
BanII         171  GGGCCC               G[GA]GC[CT]^C
              841  GAGCCC              
BcnI          727  CCCGG                CC^[GC]GG
              871  CCCGG               
BsaHI           4  GGCGTC               G[GA]^CG[CT]C
              877  GACGTC              
BseRI         549  CTCCTC               GAGGAG(10/8)
              860  CTCCTC              
BsmAI         875  GAGAC                GTCTC(1/5)
              858  GTCTC               
BsrFI          25  GCCGGC               [GA]^CCGG[CT]
              257  GCCGGC              
BstXI          11  CCACGGCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              527  CCAACTGTGTGG        
Cfr10I         25  GCCGGC               [GA]^CCGG[CT]
              257  GCCGGC              
DpnII          44  GATC                 ^GATC
              450  GATC                
EarI           52  CTCTTC               CTCTTC(1/4)
               61  CTCTTC              
HgaI            5  GCGTC                GACGC(5/10)
               73  GCGTC               
Hin1I           4  GGCGTC               G[GA]^CG[CT]C
              877  GACGTC              
Ksp632I        52  CTCTTC               CTCTTC(1/4)
               61  CTCTTC              
MaeIII        148  GTCAC                ^GT[ACGT]AC
              196  GTGAC               
MboI           44  GATC                 ^GATC
              450  GATC                
MspA1I         30  CAGCTG               C[AC]G^C[GT]G
              802  CAGCGG              
NaeI           25  GCCGGC               GCC^GGC
              257  GCCGGC              
NciI          727  CCCGG                CC^[GC]GG
              871  CCCGG               
NdeII          44  GATC                 ^GATC
              450  GATC                
NgoMIV         25  GCCGGC               G^CCGGC
              257  GCCGGC              
RsaI          216  GTAC                 GT^AC
              540  GTAC                
Sau3AI         44  GATC                 ^GATC
              450  GATC                
Tsp45I        148  GTCAC                ^GT[GC]AC
              196  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AciI        AluI        AvaI        AvaII       BanI        
BbvI        BmyI        BpmI        BsaJI       BsgI        
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BspMI       BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DraII       DsaI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeII       
MboII       MnlI        MspI        MvaI        MwoI        
NcoI        NlaIII      NlaIV       PflMI       PleI        
PstI        Sau96I      ScrFI       SfaNI       SfcI        
StyI        TseI        Van91I      
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