Restriction map for pF1KSDF0041
 (Linear) (Six-base) MAPSORT of: FLJ00041.seq  Check: 9364  from: 1  to: 933



FLJ00041.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     166     302     489     756     933 
   Size:        166     136     187     267     177

  Fragments arranged by size: 

                 267     187     177     166     136
 
AatII G_ACGT'C

Cuts at:      0     881     933 
   Size:        881      52
 
AccI GT'mk_AC

Cuts at:      0     644     933 
   Size:        644     289
 
Acc65I G'GTAC_C

Cuts at:      0     215     933 
   Size:        215     718
 
AlwNI CAG_nnn'CTG

Cuts at:      0     687     933 
   Size:        687     246
 
ApaI G_GGCC'C

Cuts at:      0     175     933 
   Size:        175     758
 
AvaI C'yCGr_G

Cuts at:      0     133     225     763     933 
   Size:        133      92     538     170

  Fragments arranged by size: 

                 538     170     133      92
 
AvrII C'CTAG_G

Cuts at:      0      78     933 
   Size:         78     855
 
BamHI G'GATC_C

Cuts at:      0      43     933 
   Size:         43     890
 
BanI G'GyrC_C

Cuts at:      0     215     455     484     595     779     933 
   Size:        215     240      29     111     184     154

  Fragments arranged by size: 

                 240     215     184     154     111      29
 
BanII G_rGCy'C

Cuts at:      0     175     845     933 
   Size:        175     670      88

  Fragments arranged by size: 

                 670     175      88
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     873     933 
   Size:        873      60
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     752     933 
   Size:        752     181
 
BglI GCCn_nnn'nGGC

Cuts at:      0      22     933 
   Size:         22     911
 
BlpI GC'TnA_GC

Cuts at:      0     341     933 
   Size:        341     592
 
Bme1580I G_kGCm'C

Cuts at:      0     175     191     681     933 
   Size:        175      16     490     252

  Fragments arranged by size: 

                 490     252     175      16
 
BmgBI CAC'GTC

Cuts at:      0     212     933 
   Size:        212     721
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     381     933 
   Size:        381     552
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     376     848     933 
   Size:         40     336     472      85

  Fragments arranged by size: 

                 472     336      85      40
 
BsaHI Gr'CG_yC

Cuts at:      0       5     878     933 
   Size:          5     873      55

  Fragments arranged by size: 

                 873      55       5
 
BsaWI w'CCGG_w

Cuts at:      0      84     933 
   Size:         84     849
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     532     562     933 
   Size:        532      30     371

  Fragments arranged by size: 

                 532     371      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     540     851     933 
   Size:        540     311      82

  Fragments arranged by size: 

                 540     311      82
 
BseYI C'CCAG_C

Cuts at:      0      38     800     844     933 
   Size:         38     762      44      89

  Fragments arranged by size: 

                 762      89      44      38
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259     280     523     933 
   Size:        259      21     243     410

  Fragments arranged by size: 

                 410     259     243      21
 
BsiHKAI G_wGCw'C

Cuts at:      0     473     933 
   Size:        473     460
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     869     933 
   Size:        869      64
 
Bsp1286I G_dGCh'C

Cuts at:      0     175     191     473     681     845     933 
   Size:        175      16     282     208     164      88

  Fragments arranged by size: 

                 282     208     175     164      88      16
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     166     253     302     489     756     933 
   Size:        166      87      49     187     267     177

  Fragments arranged by size: 

                 267     187     177     166      87      49
 
BsrFI r'CCGG_y

Cuts at:      0      25     257     933 
   Size:         25     232     676

  Fragments arranged by size: 

                 676     232      25
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      18     534     933 
   Size:         18     516     399

  Fragments arranged by size: 

                 516     399      18
 
BstYI r'GATC_y

Cuts at:      0      43     933 
   Size:         43     890
 
BtgI C'CryG_G

Cuts at:      0      11     300     348     375     480     488     933 
   Size:         11     289      48      27     105       8     445

  Fragments arranged by size: 

                 445     289     105      48      27      11       8
 
DraIII CAC_nnn'GTG

Cuts at:      0     195     933 
   Size:        195     738
 
EarI CTCTTCn'nnn_

Cuts at:      0      58      67     933 
   Size:         58       9     866

  Fragments arranged by size: 

                 866      58       9
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     376     848     933 
   Size:         40     336     472      85

  Fragments arranged by size: 

                 472     336      85      40
 
EcoO109I rG'GnC_Cy

Cuts at:      0     172     285     868     933 
   Size:        172     113     583      65

  Fragments arranged by size: 

                 583     172     113      65
 
FspI TGC'GCA

Cuts at:      0     400     933 
   Size:        400     533
 
HaeII r_GCGC'y

Cuts at:      0      39     258     348     933 
   Size:         39     219      90     585

  Fragments arranged by size: 

                 585     219      90      39
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      35      67     508     540     933 
   Size:         35      32     441      32     393

  Fragments arranged by size: 

                 441     393      35      32      32
 
KpnI G_GTAC'C

Cuts at:      0     219     933 
   Size:        219     714
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     392     551     692     933 
   Size:        392     159     141     241

  Fragments arranged by size: 

                 392     241     159     141
 
MslI CAynn'nnrTG

Cuts at:      0     394     933 
   Size:        394     539
 
MspA1I CmG'CkG

Cuts at:      0      32     804     933 
   Size:         32     772     129

  Fragments arranged by size: 

                 772     129      32
 
NaeI GCC'GGC

Cuts at:      0      27     259     933 
   Size:         27     232     674

  Fragments arranged by size: 

                 674     232      27
 
NcoI C'CATG_G

Cuts at:      0     300     375     480     488     933 
   Size:        300      75     105       8     445

  Fragments arranged by size: 

                 445     300     105      75       8
 
NgoMIV G'CCGG_C

Cuts at:      0      25     257     933 
   Size:         25     232     676

  Fragments arranged by size: 

                 676     232      25
 
PasI CC'CwG_GG

Cuts at:      0     753     933 
   Size:        753     180
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     336     354     687     933 
   Size:        336      18     333     246

  Fragments arranged by size: 

                 336     333     246      18
 
PfoI T'CCnGG_A

Cuts at:      0     281     933 
   Size:        281     652
 
PpuMI rG'GwC_Cy

Cuts at:      0     285     933 
   Size:        285     648
 
PspOMI G'GGCC_C

Cuts at:      0     171     933 
   Size:        171     762
 
PspXI vC'TCGA_Gb

Cuts at:      0     225     933 
   Size:        225     708
 
PstI C_TGCA'G

Cuts at:      0     163     500     582     933 
   Size:        163     337      82     351

  Fragments arranged by size: 

                 351     337     163      82
 
PvuII CAG'CTG

Cuts at:      0      32     933 
   Size:         32     901
 
SapI GCTCTTCn'nnn_

Cuts at:      0      58     933 
   Size:         58     875
 
SbfI CC_TGCA'GG

Cuts at:      0     163     933 
   Size:        163     770
 
SfcI C'TryA_G

Cuts at:      0     159     496     578     933 
   Size:        159     337      82     355

  Fragments arranged by size: 

                 355     337     159      82
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      22     933 
   Size:         22     911
 
SmlI C'TyrA_G

Cuts at:      0     225     933 
   Size:        225     708
 
StyI C'CwwG_G

Cuts at:      0      78     300     375     480     488     933 
   Size:         78     222      75     105       8     445

  Fragments arranged by size: 

                 445     222     105      78      75       8
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     772     804     933 
   Size:        772      32     129

  Fragments arranged by size: 

                 772     129      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     901     933 
   Size:        901      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     333     933 
   Size:        333     600
 
XhoI C'TCGA_G

Cuts at:      0     225     933 
   Size:        225     708
 
ZraI GAC'GTC

Cuts at:      0     879     933 
   Size:        879      54
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I    AlwNI     ApaI     AvaI    AvrII
    BamHI     BanI    BanII     BbsI    BciVI     BglI     BlpI Bme1580I
    BmgBI     BplI     BpmI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrFI    BstXI    BstYI
     BtgI   DraIII     EarI  Eco57MI EcoO109I     FspI    HaeII    Hin4I
     KpnI     MmeI     MslI   MspA1I     NaeI     NcoI   NgoMIV     PasI
    PflMI     PfoI    PpuMI   PspOMI    PspXI     PstI    PvuII     SapI
     SbfI     SfcI     SfiI     SmlI     StyI     TstI  Tth111I     XcmI
     XhoI     ZraI

 Enzymes that do not cut: 

     AclI     AcuI     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI     BaeI
     BbeI    BbvCI     BcgI     BclI    BfrBI    BglII     BmrI     BmtI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsiEI    BsiWI     BsmI
    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI    FspAI   HincII  HindIII     HpaI     KasI
     MfeI     MluI     MscI     NarI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     ScaI    SexAI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     XbaI
     XmaI     XmnI
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