Restriction map for
pF1KSDB1467
(Linear) (Six-base) MAPSORT of: KIBB1467.seq Check: 2395 from: 1 to: 4902
KIBB1467.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1189 2073 4902
Size: 1189 884 2829
Fragments arranged by size:
2829 1189 884
AccI GT'mk_AC
Cuts at: 0 194 1202 4902
Size: 194 1008 3700
Fragments arranged by size:
3700 1008 194
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 777 1153 1600 2210 2211 2644 2797 2893
Size: 777 376 447 610 1 433 153 96
Cuts at: 2893 3628 4902
Size: 735 1274
Fragments arranged by size:
1274 777 735 610 447 433 376 153
96 1
AflIII A'CryG_T
Cuts at: 0 3623 4902
Size: 3623 1279
AhdI GACnn_n'nnGTC
Cuts at: 0 3617 4902
Size: 3617 1285
AleI CACnn'nnGTG
Cuts at: 0 2112 4902
Size: 2112 2790
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 853 2020 3104 4902
Size: 853 1167 1084 1798
Fragments arranged by size:
1798 1167 1084 853
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2881 2913 4902
Size: 2881 32 1989
Fragments arranged by size:
2881 1989 32
AlwNI CAG_nnn'CTG
Cuts at: 0 764 1224 1509 2238 2737 2775 2919 3158
Size: 764 460 285 729 499 38 144 239
Cuts at: 3158 4902
Size: 1744
Fragments arranged by size:
1744 764 729 499 460 285 239 144
38
ApaI G_GGCC'C
Cuts at: 0 4494 4902
Size: 4494 408
ApaLI G'TGCA_C
Cuts at: 0 2306 4902
Size: 2306 2596
ApoI r'AATT_y
Cuts at: 0 1703 4628 4902
Size: 1703 2925 274
Fragments arranged by size:
2925 1703 274
AvaI C'yCGr_G
Cuts at: 0 482 1114 1837 2099 4310 4433 4902
Size: 482 632 723 262 2211 123 469
Fragments arranged by size:
2211 723 632 482 469 262 123
BamHI G'GATC_C
Cuts at: 0 617 1713 3213 3857 4730 4902
Size: 617 1096 1500 644 873 172
Fragments arranged by size:
1500 1096 873 644 617 172
BanI G'GyrC_C
Cuts at: 0 659 849 2184 4453 4894 4902
Size: 659 190 1335 2269 441 8
Fragments arranged by size:
2269 1335 659 441 190 8
BanII G_rGCy'C
Cuts at: 0 448 940 1441 2022 2158 2554 2666 4036
Size: 448 492 501 581 136 396 112 1370
Cuts at: 4036 4090 4494 4763 4902
Size: 54 404 269 139
Fragments arranged by size:
1370 581 501 492 448 404 396 269
139 136 112 54
BbeI G_GCGC'C
Cuts at: 0 663 4902
Size: 663 4239
BbsI GAAGACnn'nnnn_
Cuts at: 0 1507 2447 3616 3696 4864 4902
Size: 1507 940 1169 80 1168 38
Fragments arranged by size:
1507 1169 1168 940 80 38
BbvCI CC'TCA_GC
Cuts at: 0 1972 4902
Size: 1972 2930
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2516 2550 3274 3308 4902
Size: 2516 34 724 34 1594
Fragments arranged by size:
2516 1594 724 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 492 3912 4902
Size: 492 3420 990
Fragments arranged by size:
3420 990 492
BfrBI ATG'CAT
Cuts at: 0 4572 4902
Size: 4572 330
BglI GCCn_nnn'nGGC
Cuts at: 0 1287 1982 3007 4902
Size: 1287 695 1025 1895
Fragments arranged by size:
1895 1287 1025 695
BglII A'GATC_T
Cuts at: 0 545 2066 3308 4130 4902
Size: 545 1521 1242 822 772
Fragments arranged by size:
1521 1242 822 772 545
BlpI GC'TnA_GC
Cuts at: 0 2738 4768 4902
Size: 2738 2030 134
Fragments arranged by size:
2738 2030 134
Bme1580I G_kGCm'C
Cuts at: 0 854 1838 2310 3152 3696 4456 4494 4902
Size: 854 984 472 842 544 760 38 408
Fragments arranged by size:
984 854 842 760 544 472 408 38
BmgBI CAC'GTC
Cuts at: 0 1260 4902
Size: 1260 3642
BmrI ACTGGGnnnn_n'
Cuts at: 0 551 676 1712 2071 3119 4902
Size: 551 125 1036 359 1048 1783
Fragments arranged by size:
1783 1048 1036 551 359 125
BmtI G_CTAG'C
Cuts at: 0 73 4902
Size: 73 4829
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 397 2568 2867 4902
Size: 397 2171 299 2035
Fragments arranged by size:
2171 2035 397 299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 392 1104 1312 2414 3088 3898 4579
Size: 61 331 712 208 1102 674 810 681
Cuts at: 4579 4747 4902
Size: 168 155
Fragments arranged by size:
1102 810 712 681 674 331 208 168
155 61
Bpu10I CC'TnA_GC
Cuts at: 0 1972 4850 4902
Size: 1972 2878 52
Fragments arranged by size:
2878 1972 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 2862 2869 3871 4066 4902
Size: 217 2645 7 1002 195 836
Fragments arranged by size:
2645 1002 836 217 195 7
BsaI GGTCTCn'nnnn_
Cuts at: 0 253 987 3400 4902
Size: 253 734 2413 1502
Fragments arranged by size:
2413 1502 734 253
BsaAI yAC'GTr
Cuts at: 0 3563 4902
Size: 3563 1339
BsaBI GATnn'nnATC
Cuts at: 0 3295 4902
Size: 3295 1607
BsaHI Gr'CG_yC
Cuts at: 0 660 898 4902
Size: 660 238 4004
Fragments arranged by size:
4004 660 238
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 304 334 1177 1207 3157 3187 3490 3520
Size: 304 30 843 30 1950 30 303 30
Cuts at: 3520 4307 4337 4505 4535 4902
Size: 787 30 168 30 367
Fragments arranged by size:
1950 843 787 367 304 303 168 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 461 1015 1309 2148 3085 4234 4357
Size: 148 313 554 294 839 937 1149 123
Cuts at: 4357 4798 4902
Size: 441 104
Fragments arranged by size:
1149 937 839 554 441 313 294 148
123 104
BseYI C'CCAG_C
Cuts at: 0 176 551 639 2201 2290 2581 2585 2759
Size: 176 375 88 1562 89 291 4 174
Cuts at: 2759 3011 4276 4461 4902
Size: 252 1265 185 441
Fragments arranged by size:
1562 1265 441 375 291 252 185 176
174 89 88 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2016 2101 2731 4902
Size: 2016 85 630 2171
Fragments arranged by size:
2171 2016 630 85
BsiEI CG_ry'CG
Cuts at: 0 3369 4305 4902
Size: 3369 936 597
Fragments arranged by size:
3369 936 597
BsiHKAI G_wGCw'C
Cuts at: 0 1112 1662 2022 2158 2310 2554 2570 4090
Size: 1112 550 360 136 152 244 16 1520
Cuts at: 4090 4763 4902
Size: 673 139
Fragments arranged by size:
1520 1112 673 550 360 244 152 139
136 16
BsiWI C'GTAC_G
Cuts at: 0 3563 4902
Size: 3563 1339
BsmI GAATG_Cn'
Cuts at: 0 897 2791 4902
Size: 897 1894 2111
Fragments arranged by size:
2111 1894 897
Bsp1286I G_dGCh'C
Cuts at: 0 448 854 940 1112 1441 1662 1838 2022
Size: 448 406 86 172 329 221 176 184
Cuts at: 2022 2158 2310 2554 2570 2666 3152 3696 4036
Size: 136 152 244 16 96 486 544 340
Cuts at: 4036 4090 4456 4494 4763 4902
Size: 54 366 38 269 139
Fragments arranged by size:
544 486 448 406 366 340 329 269
244 221 184 176 172 152 139 136
96 86 54 38 16
BspHI T'CATG_A
Cuts at: 0 1329 3299 4902
Size: 1329 1970 1603
Fragments arranged by size:
1970 1603 1329
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1189 2073 2223 2762 3006 4699 4754 4902
Size: 1189 884 150 539 244 1693 55 148
Fragments arranged by size:
1693 1189 884 539 244 150 148 55
BsrBI CCG'CTC
Cuts at: 0 4307 4332 4902
Size: 4307 25 570
Fragments arranged by size:
4307 570 25
BsrDI GCAATG_nn'
Cuts at: 0 24 4902
Size: 24 4878
BsrFI r'CCGG_y
Cuts at: 0 2090 3364 3442 3458 4043 4861 4902
Size: 2090 1274 78 16 585 818 41
Fragments arranged by size:
2090 1274 818 585 78 41 16
BsrGI T'GTAC_A
Cuts at: 0 4409 4902
Size: 4409 493
BssSI C'ACGA_G
Cuts at: 0 3102 4189 4902
Size: 3102 1087 713
Fragments arranged by size:
3102 1087 713
BstEII G'GTnAC_C
Cuts at: 0 3438 4741 4902
Size: 3438 1303 161
Fragments arranged by size:
3438 1303 161
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1725 1890 3132 3670 4902
Size: 1725 165 1242 538 1232
Fragments arranged by size:
1725 1242 1232 538 165
BstYI r'GATC_y
Cuts at: 0 345 545 617 1713 2066 3213 3308 3419
Size: 345 200 72 1096 353 1147 95 111
Cuts at: 3419 3857 4130 4730 4879 4902
Size: 438 273 600 149 23
Fragments arranged by size:
1147 1096 600 438 353 345 273 200
149 111 95 72 23
Bsu36I CC'TnA_GG
Cuts at: 0 845 1246 3907 3914 4385 4902
Size: 845 401 2661 7 471 517
Fragments arranged by size:
2661 845 517 471 401 7
BtgI C'CryG_G
Cuts at: 0 818 1634 1977 4902
Size: 818 816 343 2925
Fragments arranged by size:
2925 818 816 343
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 88 4902
Size: 88 4814
BtsI GCAGTG_nn'
Cuts at: 0 1410 1838 2107 2635 2719 3981 4426 4902
Size: 1410 428 269 528 84 1262 445 476
Fragments arranged by size:
1410 1262 528 476 445 428 269 84
DrdI GACnn_nn'nnGTC
Cuts at: 0 1594 4902
Size: 1594 3308
EaeI y'GGCC_r
Cuts at: 0 77 821 963 1628 2247 2269 2999 3366
Size: 77 744 142 665 619 22 730 367
Cuts at: 3366 3557 3642 4092 4302 4325 4391 4902
Size: 191 85 450 210 23 66 511
Fragments arranged by size:
744 730 665 619 511 450 367 210
191 142 85 77 66 23 22
EagI C'GGCC_G
Cuts at: 0 3366 4302 4902
Size: 3366 936 600
Fragments arranged by size:
3366 936 600
EarI CTCTTCn'nnn_
Cuts at: 0 420 620 626 983 2010 2044 2224 2957
Size: 420 200 6 357 1027 34 180 733
Cuts at: 2957 3826 4261 4561 4902
Size: 869 435 300 341
Fragments arranged by size:
1027 869 733 435 420 357 341 300
200 180 34 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 237 4350 4816 4902
Size: 237 4113 466 86
Fragments arranged by size:
4113 466 237 86
EcoICRI GAG'CTC
Cuts at: 0 2020 2156 2552 4088 4761 4902
Size: 2020 136 396 1536 673 141
Fragments arranged by size:
2020 1536 673 396 141 136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 392 777 1104 1153 1312 1600 2210
Size: 61 331 385 327 49 159 288 610
Cuts at: 2210 2211 2414 2644 2797 2893 3088 3628 3898
Size: 1 203 230 153 96 195 540 270
Cuts at: 3898 4579 4747 4902
Size: 681 168 155
Fragments arranged by size:
681 610 540 385 331 327 288 270
230 203 195 168 159 155 153 96
61 49 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 625 1861 3070 3265 4813 4849 4902
Size: 625 1236 1209 195 1548 36 53
Fragments arranged by size:
1548 1236 1209 625 195 53 36
EcoO109I rG'GnC_Cy
Cuts at: 0 527 1506 1886 2235 2772 3882 4902
Size: 527 979 380 349 537 1110 1020
Fragments arranged by size:
1110 1020 979 537 527 380 349
EcoRV GAT'ATC
Cuts at: 0 1032 4902
Size: 1032 3870
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1335 2055 3102 4902
Size: 1335 720 1047 1800
Fragments arranged by size:
1800 1335 1047 720
FspI TGC'GCA
Cuts at: 0 1402 3337 4902
Size: 1402 1935 1565
Fragments arranged by size:
1935 1565 1402
FspAI rTGC'GCAy
Cuts at: 0 3337 4902
Size: 3337 1565
HaeII r_GCGC'y
Cuts at: 0 663 2297 2514 2579 4902
Size: 663 1634 217 65 2323
Fragments arranged by size:
2323 1634 663 217 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 94 126 476 508 1736 1768 3603 3635
Size: 94 32 350 32 1228 32 1835 32
Cuts at: 3635 4902
Size: 1267
Fragments arranged by size:
1835 1267 1228 350 94 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1128 3774 4902
Size: 1128 2646 1128
Fragments arranged by size:
2646 1128 1128
HindIII A'AGCT_T
Cuts at: 0 2254 2452 4902
Size: 2254 198 2450
Fragments arranged by size:
2450 2254 198
KasI G'GCGC_C
Cuts at: 0 659 4902
Size: 659 4243
MfeI C'AATT_G
Cuts at: 0 589 4902
Size: 589 4313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2281 4482 4902
Size: 2281 2201 420
Fragments arranged by size:
2281 2201 420
MscI TGG'CCA
Cuts at: 0 823 965 2271 3001 3559 4902
Size: 823 142 1306 730 558 1343
Fragments arranged by size:
1343 1306 823 730 558 142
MslI CAynn'nnrTG
Cuts at: 0 2112 2651 3337 4564 4567 4902
Size: 2112 539 686 1227 3 335
Fragments arranged by size:
2112 1227 686 539 335 3
MspA1I CmG'CkG
Cuts at: 0 176 786 1380 1671 1771 2178 2585 2763
Size: 176 610 594 291 100 407 407 178
Cuts at: 2763 3353 4209 4755 4767 4902
Size: 590 856 546 12 135
Fragments arranged by size:
856 610 594 590 546 407 407 291
178 176 135 100 12
NarI GG'CG_CC
Cuts at: 0 660 4902
Size: 660 4242
NcoI C'CATG_G
Cuts at: 0 1977 4902
Size: 1977 2925
NheI G'CTAG_C
Cuts at: 0 69 4902
Size: 69 4833
NsiI A_TGCA'T
Cuts at: 0 4574 4902
Size: 4574 328
NspI r_CATG'y
Cuts at: 0 1026 1318 1695 2674 3276 3336 3627 4902
Size: 1026 292 377 979 602 60 291 1275
Fragments arranged by size:
1275 1026 979 602 377 292 291 60
PasI CC'CwG_GG
Cuts at: 0 2196 2658 3152 3219 3493 4875 4902
Size: 2196 462 494 67 274 1382 27
Fragments arranged by size:
2196 1382 494 462 274 67 27
PciI A'CATG_T
Cuts at: 0 3623 4902
Size: 3623 1279
PflMI CCAn_nnn'nTGG
Cuts at: 0 337 764 1026 1931 3210 4011 4902
Size: 337 427 262 905 1279 801 891
Fragments arranged by size:
1279 905 891 801 427 337 262
PfoI T'CCnGG_A
Cuts at: 0 1862 2432 2681 3020 3317 4226 4595 4902
Size: 1862 570 249 339 297 909 369 307
Fragments arranged by size:
1862 909 570 369 339 307 297 249
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4062 4094 4902
Size: 4062 32 808
Fragments arranged by size:
4062 808 32
PpuMI rG'GwC_Cy
Cuts at: 0 527 1886 2235 3882 4902
Size: 527 1359 349 1647 1020
Fragments arranged by size:
1647 1359 1020 527 349
PspOMI G'GGCC_C
Cuts at: 0 4490 4902
Size: 4490 412
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3517 3549 4902
Size: 3517 32 1353
Fragments arranged by size:
3517 1353 32
PstI C_TGCA'G
Cuts at: 0 92 927 1307 2009 2345 3287 3482 4751
Size: 92 835 380 702 336 942 195 1269
Cuts at: 4751 4902
Size: 151
Fragments arranged by size:
1269 942 835 702 380 336 195 151
92
PvuII CAG'CTG
Cuts at: 0 176 1380 1671 2585 2763 4767 4902
Size: 176 1204 291 914 178 2004 135
Fragments arranged by size:
2004 1204 914 291 178 176 135
RsrII CG'GwC_CG
Cuts at: 0 224 4902
Size: 224 4678
SacI G_AGCT'C
Cuts at: 0 2022 2158 2554 4090 4763 4902
Size: 2022 136 396 1536 673 139
Fragments arranged by size:
2022 1536 673 396 139 136
SanDI GG'GwC_CC
Cuts at: 0 527 4902
Size: 527 4375
SapI GCTCTTCn'nnn_
Cuts at: 0 2010 4261 4902
Size: 2010 2251 641
Fragments arranged by size:
2251 2010 641
SexAI A'CCwGG_T
Cuts at: 0 1784 1856 4902
Size: 1784 72 3046
Fragments arranged by size:
3046 1784 72
SfcI C'TryA_G
Cuts at: 0 88 923 1199 1303 1375 2005 2341 3283
Size: 88 835 276 104 72 630 336 942
Cuts at: 3283 3478 4747 4902
Size: 195 1269 155
Fragments arranged by size:
1269 942 835 630 336 276 195 155
104 88 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3007 4902
Size: 3007 1895
SfoI GGC'GCC
Cuts at: 0 661 4902
Size: 661 4241
SmaI CCC'GGG
Cuts at: 0 1839 4312 4435 4902
Size: 1839 2473 123 467
Fragments arranged by size:
2473 1839 467 123
SmlI C'TyrA_G
Cuts at: 0 232 2841 2884 3886 4081 4902
Size: 232 2609 43 1002 195 821
Fragments arranged by size:
2609 1002 821 232 195 43
SphI G_CATG'C
Cuts at: 0 1318 1695 2674 4902
Size: 1318 377 979 2228
Fragments arranged by size:
2228 1318 979 377
StuI AGG'CCT
Cuts at: 0 1479 1870 3912 4902
Size: 1479 391 2042 990
Fragments arranged by size:
2042 1479 990 391
StyI C'CwwG_G
Cuts at: 0 324 1319 1977 4736 4902
Size: 324 995 658 2759 166
Fragments arranged by size:
2759 995 658 324 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 241 1249 1252 1927 3211 3599 3829 4439
Size: 241 1008 3 675 1284 388 230 610
Cuts at: 4439 4902
Size: 463
Fragments arranged by size:
1284 1008 675 610 463 388 241 230
3
TatI w'GTAC_w
Cuts at: 0 1334 1796 2483 4409 4902
Size: 1334 462 687 1926 493
Fragments arranged by size:
1926 1334 687 493 462
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 19 51 304 336 803 835 2300 2332
Size: 19 32 253 32 467 32 1465 32
Cuts at: 2332 4307 4339 4505 4537 4902
Size: 1975 32 166 32 365
Fragments arranged by size:
1975 1465 467 365 253 166 32 32
32 32 32 32 19
Tth111I GACn'n_nGTC
Cuts at: 0 1858 2332 4902
Size: 1858 474 2570
Fragments arranged by size:
2570 1858 474
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1782 3430 3567 3637 4902
Size: 1782 1648 137 70 1265
Fragments arranged by size:
1782 1648 1265 137 70
XmaI C'CCGG_G
Cuts at: 0 1837 4310 4433 4902
Size: 1837 2473 123 469
Fragments arranged by size:
2473 1837 469 123
XmnI GAAnn'nnTTC
Cuts at: 0 3082 4132 4902
Size: 3082 1050 770
Fragments arranged by size:
3082 1050 770
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FspI
FspAI HaeII Hin4I HincII HindIII KasI MfeI MmeI
MscI MslI MspA1I NarI NcoI NheI NsiI NspI
PasI PciI PflMI PfoI PpiI PpuMI PspOMI PsrI
PstI PvuII RsrII SacI SanDI SapI SexAI SfcI
SfiI SfoI SmaI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AscI AseI
AsiSI AvrII BaeI BclI BsaWI BsmBI BspEI BssHII
BstAPI BstBI BstZ17I ClaI CspCI DraI DraIII EcoRI
FseI HpaI KpnI MluI NaeI NdeI NgoMIV NotI
NruI PacI PmeI PmlI PshAI PsiI PspXI PvuI
SacII SalI SbfI ScaI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI XbaI XhoI ZraI