Restriction map for pF1KSDB1453
 (Linear) (Six-base) MAPSORT of: KIBB1453.seq  Check: 5959  from: 1  to: 1395



KIBB1453.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:19  ..

AatII G_ACGT'C

Cuts at:      0     828    1395 
   Size:        828     567
 
AfeI AGC'GCT

Cuts at:      0    1339    1395 
   Size:       1339      56
 
AflII C'TTAA_G

Cuts at:      0    1255    1395 
   Size:       1255     140
 
AflIII A'CryG_T

Cuts at:      0     336    1328    1395 
   Size:        336     992      67

  Fragments arranged by size: 

                 992     336      67
 
AleI CACnn'nnGTG

Cuts at:      0     401    1395 
   Size:        401     994
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     772     804    1395 
   Size:        772      32     591

  Fragments arranged by size: 

                 772     591      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1246    1395 
   Size:       1246     149
 
ApoI r'AATT_y

Cuts at:      0      84     730     786    1395 
   Size:         84     646      56     609

  Fragments arranged by size: 

                 646     609      84      56
 
BanII G_rGCy'C

Cuts at:      0    1110    1395 
   Size:       1110     285
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1308    1395 
   Size:       1308      87
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      84     118    1395 
   Size:         84      34    1277

  Fragments arranged by size: 

                1277      84      34
 
BclI T'GATC_A

Cuts at:      0     929    1395 
   Size:        929     466
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     755    1167    1320    1395 
   Size:        755     412     153      75

  Fragments arranged by size: 

                 755     412     153      75
 
BmtI G_CTAG'C

Cuts at:      0     835    1395 
   Size:        835     560
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     530    1016    1133    1395 
   Size:        100     430     486     117     262

  Fragments arranged by size: 

                 486     430     262     117     100
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     965    1395 
   Size:        965     430
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     967    1170    1395 
   Size:        967     203     225

  Fragments arranged by size: 

                 967     225     203
 
BsaAI yAC'GTr

Cuts at:      0    1331    1395 
   Size:       1331      64
 
BsaBI GATnn'nnATC

Cuts at:      0     946    1395 
   Size:        946     449
 
BsaHI Gr'CG_yC

Cuts at:      0     825    1395 
   Size:        825     570
 
BsaWI w'CCGG_w

Cuts at:      0     191    1395 
   Size:        191    1204
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     553     583    1014    1044    1395 
   Size:        553      30     431      30     351

  Fragments arranged by size: 

                 553     431     351      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     193     305     574    1395 
   Size:        193     112     269     821

  Fragments arranged by size: 

                 821     269     193     112
 
BsiHKAI G_wGCw'C

Cuts at:      0    1110    1395 
   Size:       1110     285
 
Bsp1286I G_dGCh'C

Cuts at:      0    1110    1395 
   Size:       1110     285
 
BspEI T'CCGG_A

Cuts at:      0     191    1395 
   Size:        191    1204
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     378    1188    1395 
   Size:        378     810     207

  Fragments arranged by size: 

                 810     378     207
 
BsrDI GCAATG_nn'

Cuts at:      0      11     353     863    1395 
   Size:         11     342     510     532

  Fragments arranged by size: 

                 532     510     342      11
 
BstYI r'GATC_y

Cuts at:      0    1062    1395 
   Size:       1062     333
 
Bsu36I CC'TnA_GG

Cuts at:      0     872    1395 
   Size:        872     523
 
EaeI y'GGCC_r

Cuts at:      0     393    1268    1395 
   Size:        393     875     127

  Fragments arranged by size: 

                 875     393     127
 
EarI CTCTTCn'nnn_

Cuts at:      0     232     551    1395 
   Size:        232     319     844

  Fragments arranged by size: 

                 844     319     232
 
EcoICRI GAG'CTC

Cuts at:      0    1108    1395 
   Size:       1108     287
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     530    1016    1133    1395 
   Size:        100     430     486     117     262

  Fragments arranged by size: 

                 486     430     262     117     100
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1347    1395 
   Size:       1347      48
 
EcoRI G'AATT_C

Cuts at:      0     786    1395 
   Size:        786     609
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1154    1395 
   Size:       1154     241
 
HaeII r_GCGC'y

Cuts at:      0    1341    1395 
   Size:       1341      54
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     253     285     901     933    1014    1046    1204    1236 
   Size:        253      32     616      32      81      32     158      32

Cuts at:   1236    1395 
   Size:        159

  Fragments arranged by size: 

                 616     253     159     158      81      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1155    1395 
   Size:       1155     240
 
MscI TGG'CCA

Cuts at:      0     395    1270    1395 
   Size:        395     875     125

  Fragments arranged by size: 

                 875     395     125
 
MslI CAynn'nnrTG

Cuts at:      0     341     401     421    1395 
   Size:        341      60      20     974

  Fragments arranged by size: 

                 974     341      60      20
 
NheI G'CTAG_C

Cuts at:      0     831    1395 
   Size:        831     564
 
NspI r_CATG'y

Cuts at:      0     208     340    1395 
   Size:        208     132    1055

  Fragments arranged by size: 

                1055     208     132
 
PciI A'CATG_T

Cuts at:      0     336    1395 
   Size:        336    1059
 
PfoI T'CCnGG_A

Cuts at:      0    1110    1395 
   Size:       1110     285
 
PshAI GACnn'nnGTC

Cuts at:      0     692    1395 
   Size:        692     703
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     299     331    1395 
   Size:        299      32    1064

  Fragments arranged by size: 

                1064     299      32
 
SacI G_AGCT'C

Cuts at:      0    1110    1395 
   Size:       1110     285
 
SfcI C'TryA_G

Cuts at:      0     459    1395 
   Size:        459     936
 
SmlI C'TyrA_G

Cuts at:      0     980    1255    1395 
   Size:        980     275     140

  Fragments arranged by size: 

                 980     275     140
 
StuI AGG'CCT

Cuts at:      0     167    1395 
   Size:        167    1228
 
StyI C'CwwG_G

Cuts at:      0    1058    1395 
   Size:       1058     337
 
Tth111I GACn'n_nGTC

Cuts at:      0    1312    1395 
   Size:       1312      83
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      75    1395 
   Size:         75    1320
 
XmnI GAAnn'nnTTC

Cuts at:      0     454    1395 
   Size:        454     941
 
ZraI GAC'GTC

Cuts at:      0     826    1395 
   Size:        826     569
 

 Enzymes that do cut:

    AatII     AfeI    AflII   AflIII     AleI     AloI    AlwNI     ApoI
    BanII     BbsI     BcgI     BclI     BmrI     BmtI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI  BsiHKAI
 Bsp1286I    BspEI    BspMI    BsrDI    BstYI   Bsu36I     EaeI     EarI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII
     MscI     MslI     NheI     NspI     PciI     PfoI    PshAI     PsrI
     SacI     SfcI     SmlI     StuI     StyI  Tth111I     XcmI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI     AgeI     AhdI     AlfI
     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI     BbeI    BbvCI    BciVI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BplI   Bpu10I    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspHI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
    EcoNI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NotI     NruI     NsiI     PacI     PasI    PflMI
     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PstI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TatI     TstI
     XbaI     XhoI     XmaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com