Restriction map for
pF1KSDB1384
(Circular) (Six-base) MAPSORT of: pF1KSDB1384.seq Check: 42 from: 1 to: 5159
pF1KSDB1384 5159 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1534 1534
Size: 5159
AccI GT'mk_AC
Cuts at: 1931 2197 1931
Size: 266 4893
Acc65I G'GTAC_C
Cuts at: 2175 2175
Size: 5159
AclI AA'CG_TT
Cuts at: 4999 4999
Size: 5159
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1379 2984 3416 4163 4431 1379
Size: 1605 432 747 268 2107
Fragments arranged by size:
2107 1605 747 432 268
AflIII A'CryG_T
Cuts at: 2511 3615 4592 2511
Size: 1104 977 3078
Fragments arranged by size:
3078 1104 977
AgeI A'CCGG_T
Cuts at: 3547 3547
Size: 5159
AleI CACnn'nnGTG
Cuts at: 136 773 136
Size: 637 4522
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2117 2117
Size: 5159
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 772 804 772
Size: 32 5127
AlwNI CAG_nnn'CTG
Cuts at: 297 1128 1263 1591 2464 4031 297
Size: 831 135 328 873 1567 1425
Fragments arranged by size:
1567 1425 873 831 328 135
ApaLI G'TGCA_C
Cuts at: 3929 3929
Size: 5159
ApoI r'AATT_y
Cuts at: 801 1338 1508 1609 1716 2163 4512 4768 801
Size: 537 170 101 107 447 2349 256 1192
Fragments arranged by size:
2349 1192 537 447 256 170 107 101
AseI AT'TA_AT
Cuts at: 20 20
Size: 5159
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5159
AvaI C'yCGr_G
Cuts at: 2179 2179
Size: 5159
BamHI G'GATC_C
Cuts at: 2184 2184
Size: 5159
BanI G'GyrC_C
Cuts at: 2175 2589 2837 2872 2175
Size: 414 248 35 4462
Fragments arranged by size:
4462 414 248 35
BanII G_rGCy'C
Cuts at: 1462 2173 3203 1462
Size: 711 1030 3418
Fragments arranged by size:
3418 1030 711
BbeI G_GCGC'C
Cuts at: 2593 2841 2593
Size: 248 4911
BbsI GAAGACnn'nnnn_
Cuts at: 1167 1298 1167
Size: 131 5028
BbvCI CC'TCA_GC
Cuts at: 461 1180 1550 1646 461
Size: 719 370 96 3974
Fragments arranged by size:
3974 719 370 96
BciVI GTATCCnnnnn_n'
Cuts at: 983 3057 3818 5159 983
Size: 2074 761 1341 983
Fragments arranged by size:
2074 1341 983 761
BclI T'GATC_A
Cuts at: 1593 2679 1593
Size: 1086 4073
BfrBI ATG'CAT
Cuts at: 1806 1806
Size: 5159
BglI GCCn_nnn'nGGC
Cuts at: 1015 1015
Size: 5159
BglII A'GATC_T
Cuts at: 745 2674 4711 745
Size: 1929 2037 1193
Fragments arranged by size:
2037 1929 1193
BlpI GC'TnA_GC
Cuts at: 2271 2271
Size: 5159
Bme1580I G_kGCm'C
Cuts at: 2784 2877 3933 2784
Size: 93 1056 4010
Fragments arranged by size:
4010 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1312 1664 2371 2452 2787 4931 1312
Size: 352 707 81 335 2144 1540
Fragments arranged by size:
2144 1540 707 352 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3526 3526
Size: 5159
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 411 662 1328 213
Size: 198 251 666 4044
Fragments arranged by size:
4044 666 251 198
Bpu10I CC'TnA_GC
Cuts at: 461 1180 1550 1646 461
Size: 719 370 96 3974
Fragments arranged by size:
3974 719 370 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2335 3214 3706 4004 2335
Size: 879 492 298 3490
Fragments arranged by size:
3490 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4863 4863
Size: 5159
BsaAI yAC'GTr
Cuts at: 2151 2512 2151
Size: 361 4798
BsaBI GATnn'nnATC
Cuts at: 6 2673 4710 6
Size: 2667 2037 455
Fragments arranged by size:
2667 2037 455
BsaHI Gr'CG_yC
Cuts at: 2590 2838 3540 5036 2590
Size: 248 702 1496 2713
Fragments arranged by size:
2713 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2348 2869 3547 3821 3968 4379 2348
Size: 521 678 274 147 411 3128
Fragments arranged by size:
3128 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2584 3342 3919 4415 2584
Size: 758 577 496 3328
Fragments arranged by size:
3328 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 549 225
Size: 324 4835
BsiEI CG_ry'CG
Cuts at: 104 137 2701 2747 3955 104
Size: 33 2564 46 1208 1308
Fragments arranged by size:
2564 1308 1208 46 33
BsiHKAI G_wGCw'C
Cuts at: 2173 2951 3141 3933 2173
Size: 778 190 792 3399
Fragments arranged by size:
3399 792 778 190
BsmI GAATG_Cn'
Cuts at: 2023 2091 2023
Size: 68 5091
Bsp1286I G_dGCh'C
Cuts at: 1462 2173 2784 2877 2951 3141 3203 3933 1462
Size: 711 611 93 74 190 62 730 2688
Fragments arranged by size:
2688 730 711 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4379 4379
Size: 5159
BspHI T'CATG_A
Cuts at: 4335 4335
Size: 5159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 149 613 873 1534 2209 2423 2725 3106 149
Size: 464 260 661 675 214 302 381 2202
Fragments arranged by size:
2202 675 661 464 381 302 260 214
BsrBI CCG'CTC
Cuts at: 50 3451 3505 4989 5154 50
Size: 3401 54 1484 165 55
Fragments arranged by size:
3401 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 500 3071 500
Size: 2571 2588
BsrFI r'CCGG_y
Cuts at: 143 154 3157 3338 3547 143
Size: 11 3003 181 209 1755
Fragments arranged by size:
3003 1755 209 181 11
BssSI C'ACGA_G
Cuts at: 3430 3788 3430
Size: 358 4801
BstAPI GCAn_nnn'nTGC
Cuts at: 471 2536 471
Size: 2065 3094
BstBI TT'CG_AA
Cuts at: 110 3520 110
Size: 3410 1749
BstEII G'GTnAC_C
Cuts at: 1535 1535
Size: 5159
BstXI CCAn_nnnn'nTGG
Cuts at: 1192 1577 1192
Size: 385 4774
BstYI r'GATC_y
Cuts at: 1 745 2184 2651 2674 3009 3255 4267 4353
Size: 744 1439 467 23 335 246 1012 86
Cuts at: 4353 4365 4711 1
Size: 12 346 449
Fragments arranged by size:
1439 1012 744 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 131 125
Size: 6 5153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3109 3290 3453 3109
Size: 181 163 4815
Fragments arranged by size:
4815 181 163
BtsI GCAGTG_nn'
Cuts at: 530 854 1915 2402 530
Size: 324 1061 487 3287
Fragments arranged by size:
3287 1061 487 324
DraI TTT'AAA
Cuts at: 1244 2158 1244
Size: 914 4245
DrdI GACnn_nn'nnGTC
Cuts at: 2865 3723 2865
Size: 858 4301
EaeI y'GGCC_r
Cuts at: 128 134 2744 2918 3309 3336 4681 128
Size: 6 2610 174 391 27 1345 606
Fragments arranged by size:
2610 1345 606 391 174 27 6
EagI C'GGCC_G
Cuts at: 134 2744 134
Size: 2610 2549
EarI CTCTTCn'nnn_
Cuts at: 1000 3182 3392 1000
Size: 2182 210 2767
Fragments arranged by size:
2767 2182 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3367 3677 3823 4969 3367
Size: 310 146 1146 3557
Fragments arranged by size:
3557 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2171 2171
Size: 5159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 411 662 1328 1379 2984 3416 4163 4431
Size: 198 251 666 51 1605 432 747 268
Cuts at: 4431 213
Size: 941
Fragments arranged by size:
1605 941 747 666 432 268 251 198
51
EcoO109I rG'GnC_Cy
Cuts at: 1125 1469 2298 1125
Size: 344 829 3986
Fragments arranged by size:
3986 829 344
EcoRI G'AATT_C
Cuts at: 2163 2163
Size: 5159
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1737 3178 1737
Size: 1441 3718
FspI TGC'GCA
Cuts at: 599 2940 599
Size: 2341 2818
HaeII r_GCGC'y
Cuts at: 2593 2841 3863 4842 2593
Size: 248 1022 979 2910
Fragments arranged by size:
2910 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 441 473 737 769 1257 1289 3001 3031 3033
Size: 32 264 32 488 32 1712 30 2
Cuts at: 3033 3063 441
Size: 30 2537
Fragments arranged by size:
2537 1712 488 264 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 684 1913 1932 2198 684
Size: 1229 19 266 3645
Fragments arranged by size:
3645 1229 266 19
HindIII A'AGCT_T
Cuts at: 842 1945 842
Size: 1103 4056
HpaI GTT'AAC
Cuts at: 684 684
Size: 5159
KasI G'GCGC_C
Cuts at: 2589 2837 2589
Size: 248 4911
KpnI G_GTAC'C
Cuts at: 2179 2179
Size: 5159
MfeI C'AATT_G
Cuts at: 1206 1206
Size: 5159
MluI A'CGCG_T
Cuts at: 4592 4592
Size: 5159
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3830 4014 3830
Size: 184 4975
MscI TGG'CCA
Cuts at: 130 2920 130
Size: 2790 2369
MslI CAynn'nnrTG
Cuts at: 136 773 859 875 2084 3148 3275 136
Size: 637 86 16 1209 1064 127 2020
Fragments arranged by size:
2020 1209 1064 637 127 86 16
MspA1I CmG'CkG
Cuts at: 2270 2584 2944 3957 4202 2270
Size: 314 360 1013 245 3227
Fragments arranged by size:
3227 1013 360 314 245
NaeI GCC'GGC
Cuts at: 145 156 3340 145
Size: 11 3184 1964
Fragments arranged by size:
3184 1964 11
NarI GG'CG_CC
Cuts at: 2590 2838 2590
Size: 248 4911
NcoI C'CATG_G
Cuts at: 125 125
Size: 5159
NgoMIV G'CCGG_C
Cuts at: 143 154 3338 143
Size: 11 3184 1964
Fragments arranged by size:
3184 1964 11
NsiI A_TGCA'T
Cuts at: 1808 1808
Size: 5159
NspI r_CATG'y
Cuts at: 2212 2712 3619 2212
Size: 500 907 3752
Fragments arranged by size:
3752 907 500
PasI CC'CwG_GG
Cuts at: 534 534
Size: 5159
PciI A'CATG_T
Cuts at: 3615 3615
Size: 5159
PflMI CCAn_nnn'nTGG
Cuts at: 845 845
Size: 5159
PmeI GTTT'AAAC
Cuts at: 2158 2158
Size: 5159
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4298 4330 4298
Size: 32 5127
PpuMI rG'GwC_Cy
Cuts at: 1125 1469 1125
Size: 344 4815
PsiI TTA'TAA
Cuts at: 256 256
Size: 5159
PstI C_TGCA'G
Cuts at: 525 2106 2206 2891 525
Size: 1581 100 685 2793
Fragments arranged by size:
2793 1581 685 100
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5159
PvuII CAG'CTG
Cuts at: 2584 2944 2584
Size: 360 4799
RsrII CG'GwC_CG
Cuts at: 3354 3354
Size: 5159
SacI G_AGCT'C
Cuts at: 2173 2173
Size: 5159
SalI G'TCGA_C
Cuts at: 1930 2196 1930
Size: 266 4893
SanDI GG'GwC_CC
Cuts at: 1469 1469
Size: 5159
SapI GCTCTTCn'nnn_
Cuts at: 3182 3392 3182
Size: 210 4949
SbfI CC_TGCA'GG
Cuts at: 2206 2206
Size: 5159
ScaI AGT'ACT
Cuts at: 1939 4676 1939
Size: 2737 2422
SexAI A'CCwGG_T
Cuts at: 1188 1188
Size: 5159
SfcI C'TryA_G
Cuts at: 33 521 657 663 2102 2202 2887 3880 4071
Size: 488 136 6 1439 100 685 993 191
Cuts at: 4071 33
Size: 1121
Fragments arranged by size:
1439 1121 993 685 488 191 136 100
6
SfoI GGC'GCC
Cuts at: 2591 2839 2591
Size: 248 4911
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5159
SmaI CCC'GGG
Cuts at: 2181 2181
Size: 5159
SmlI C'TyrA_G
Cuts at: 2314 3229 3721 3983 2314
Size: 915 492 262 3490
Fragments arranged by size:
3490 915 492 262
SnaBI TAC'GTA
Cuts at: 2151 2151
Size: 5159
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5159
SphI G_CATG'C
Cuts at: 2212 2712 2212
Size: 500 4659
SspI AAT'ATT
Cuts at: 1147 1147
Size: 5159
StuI AGG'CCT
Cuts at: 1771 1771
Size: 5159
StyI C'CwwG_G
Cuts at: 125 2293 2647 125
Size: 2168 354 2637
Fragments arranged by size:
2637 2168 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1778 2742 2875 3334 3543 1778
Size: 964 133 459 209 3394
Fragments arranged by size:
3394 964 459 209 133
TatI w'GTAC_w
Cuts at: 542 1937 4674 542
Size: 1395 2737 1027
Fragments arranged by size:
2737 1395 1027
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 160 192 1976 2008 4676 4708 160
Size: 32 1784 32 2668 32 611
Fragments arranged by size:
2668 1784 611 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2956 2956
Size: 5159
XbaI T'CTAG_A
Cuts at: 2190 2190
Size: 5159
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2654 2654
Size: 5159
XmaI C'CCGG_G
Cuts at: 2179 2179
Size: 5159
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI ApaI AscI AvrII BaeI
BcgI BmgBI BmtI BsaXI BseRI BsiWI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI NdeI NheI NotI NruI PacI PfoI
PmlI PshAI PspOMI PspXI PsrI SacII SfiI SgrAI
SrfI SwaI XhoI XmnI ZraI