Restriction map for pF1KE2504
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AclI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AseI        
AsnI        AspEI       AvrII       BanII       BbeI        
BbrPI       BbvCI       BclI        BfrI        BglII       
BlnI        BsiEI       BsiWI       Bsp1407I    BsrDI       
BsrFI       BsrGI       BssSI       Bst1107I    BstBI       
BstZ17I     Bsu36I      Cfr10I      CpoI        DraI        
DraIII      EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoT22I     FbaI        FseI        
HgaI        HpaI        KasI        KspI        MfeI        
MluI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Van91I      XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI         2899  GTAGAC               GT^[AC][GT]AC
AccII          52  CGCG                 CG^CG
AccIII         98  TCCGGA               T^CCGGA
AscI           50  GGCGCGCC             GG^CGCGCC
Asp700I      1650  GAAGGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        1834  GAGCAC               G[AT]GC[AT]^C
AspI         2695  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1626  TGCGCA               TGC^GCA
BciVI        1899  GTATCC               GTATCC(6/5)
BfaI          451  CTAG                 C^TAG
BlpI         2100  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2100  GCTGAGC              GC^T[ACGT]AGC
BsaAI         369  TACGTG               [CT]AC^GT[GA]
BsaBI         102  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI          98  TCCGGA               T^CCGGA
BsiHKAI      1834  GAGCAC               G[AT]GC[AT]^C
BspEI          98  TCCGGA               T^CCGGA
BspHI        1841  TCATGA               T^CATGA
BspMI        2300  ACCTGC               ACCTGC(4/8)
BssHII         51  GCGCGC               G^CGCGC
BstEII        999  GGTAACC              G^GT[ACGT]ACC
BstPI         999  GGTAACC              G^GT[ACGT]ACC
BstUI          52  CGCG                 CG^CG
CelII        2100  GCTGAGC              GC^T[ACGT]AGC
DrdI         2488  GACTTGGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1945  GAAGAG               CTCTTC(1/4)
EcoO65I       999  GGTAACC              G^GT[ACGT]ACC
EcoRI        1012  GAATTC               G^AATTC
EcoRV        2503  GATATC               GAT^ATC
FspI         1626  TGCGCA               TGC^GCA
HindIII       697  AAGCTT               A^AGCTT
Ksp632I      1945  GAAGAG               CTCTTC(1/4)
MaeI          451  CTAG                 C^TAG
MamI          102  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI           98  TCCGGA               T^CCGGA
MvnI           52  CGCG                 CG^CG
NcoI         2054  CCATGG               C^CATGG
PflFI        2695  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI         1714  CTGCAG               CTGCA^G
RcaI         1841  TCATGA               T^CATGA
Tth111I      2695  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI         1650  GAAGGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AatII         940  GACGTC               GACGT^C
             1381  GACGTC              
Acc65I        152  GGTACC               G^GTACC
              470  GGTACC              
AcyI          940  GACGTC               G[GA]^CG[CT]C
             1381  GACGTC              
AflIII       2666  ACATGT               A^C[GA][CT]GT
             2783  ACATGT              
Asp718I       152  GGTACC               G^GTACC
              470  GGTACC              
BalI          104  TGGCCA               TGG^CCA
             1935  TGGCCA              
BcgI          522  CGAGTACAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              857  GCATGGTGTTCG        
BmyI          869  GTGCCC               G[AGT]GC[ACT]^C
             1834  GAGCAC              
BsaHI         940  GACGTC               G[GA]^CG[CT]C
             1381  GACGTC              
BsaI         2738  GAGACC               GGTCTC(1/5)
             2700  GGTCTC              
BsaWI          98  TCCGGA               [AT]^CCGG[AT]
              986  ACCGGA              
BsmBI        1383  CGTCTC               CGTCTC(1/5)
             2729  CGTCTC              
BsmI          889  GAATGC               GAATGC(1/-1)
             2530  GAATGC              
Bsp1286I      869  GTGCCC               G[AGT]GC[ACT]^C
             1834  GAGCAC              
BspDI        2368  ATCGAT               AT^CGAT
             2500  ATCGAT              
BspLU11I     2666  ACATGT               A^CATGT
             2783  ACATGT              
BsrBI        2248  CCGCTC               CCGCTC(-3/-3)
               45  GAGCGG              
BstXI         956  CCAAGTGTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2223  CCACGACTTTGG        
ClaI         2368  ATCGAT               AT^CGAT
             2500  ATCGAT              
DraII        1759  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             2009  AGGACCT             
DsaI         1091  CCGTGG               C^C[GA][CT]GG
             2054  CCATGG              
EcoNI        2136  CCTCAGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2616  CCTGTCCCAGG         
EcoO109I     1759  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             2009  AGGACCT             
FauI         2640  CCCGC                CCCGC(4/6)
               47  GCGGG               
HaeII        1084  AGCGCC               [GA]GCGC^[CT]
             2649  AGCGCC              
Hin1I         940  GACGTC               G[GA]^CG[CT]C
             1381  GACGTC              
KpnI          152  GGTACC               GGTAC^C
              470  GGTACC              
MluNI         104  TGGCCA               TGG^CCA
             1935  TGGCCA              
MscI          104  TGGCCA               TGG^CCA
             1935  TGGCCA              
PpuMI        1759  AGGTCCT              [GA]G^G[AT]CC[CT]
             2009  AGGACCT             
PvuII         901  CAGCTG               CAG^CTG
             1592  CAGCTG              
SmlI          541  CTCAAG               C^T[CT][GA]AG
             1033  CTCAAG              
TfiI         1606  GATTC                G^A[AT]TC
             2419  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AvaI        AvaII       BamHI       
BanI        BbsI        BbvI        BcnI        BglI        
BpmI        BpuAI       BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       EaeI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII       
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