Restriction map for
pF1KSDB0064
##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AcyI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI Asp700I Asp718I AspEI
AspI AvrII BalI BbeI BbrPI
BbvCI BciVI BclI BfrI BglII
BlnI BsaAI BsaBI BsaHI BsaI
BsiEI BsiWI BsmBI Bsp1407I BspHI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I Cfr10I CpoI DraI DraIII
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoT22I
FbaI FseI HaeII HgaI Hin1I
HindIII KasI KpnI Ksp632I KspI
MamI MluI MluNI MscI MslI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I Van91I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 1514 CGCG CG^CG
AccIII 984 TCCGGA T^CCGGA
AclI 2020 AACGTT AA^CGTT
AlwNI 2182 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1302 GGGCCC GGGCC^C
AseI 700 ATTAAT AT^TAAT
AsnI 700 ATTAAT AT^TAAT
AviII 2120 TGCGCA TGC^GCA
BamHI 802 GGATCC G^GATCC
BanI 198 GGCACC G^G[CT][GA]CC
BcgI 1751 GCAAATGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 625 CTAG C^TAG
BlpI 1162 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1162 GCTGAGC GC^T[ACGT]AGC
BseAI 984 TCCGGA T^CCGGA
BsmAI 177 GTCTC GTCTC(1/5)
BspDI 2441 ATCGAT AT^CGAT
BspEI 984 TCCGGA T^CCGGA
BspLU11I 975 ACATGT A^CATGT
BsrBI 1129 CCGCTC CCGCTC(-3/-3)
BstUI 1514 CGCG CG^CG
BstYI 802 GGATCC [GA]^GATC[CT]
CelII 1162 GCTGAGC GC^T[ACGT]AGC
ClaI 2441 ATCGAT AT^CGAT
DrdI 2200 GACGGAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1670 CCATGG C^C[GA][CT]GG
EaeI 1414 CGGCCA [CT]^GGCC[GA]
EcoRV 2284 GATATC GAT^ATC
FauI 1515 GCGGG CCCGC(4/6)
FspI 2120 TGCGCA TGC^GCA
MaeI 625 CTAG C^TAG
MfeI 815 CAATTG C^AATTG
MflI 802 GGATCC [GA]^GATC[CT]
MroI 984 TCCGGA T^CCGGA
MunI 815 CAATTG C^AATTG
MvnI 1514 CGCG CG^CG
NcoI 1670 CCATGG C^CATGG
PshBI 700 ATTAAT AT^TAAT
Psp1406I 2020 AACGTT AA^CGTT
PstI 2471 CTGCAG CTGCA^G
SacI 1676 GAGCTC GAGCT^C
SmaI 492 CCCGGG CCC^GGG
SmlI 1554 CTTGAG C^T[CT][GA]AG
SspI 2265 AATATT AAT^ATT
XhoII 802 GGATCC [GA]^GATC[CT]
XmaCI 492 CCCGGG C^CCGGG
XmaI 492 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 975 ACATGT A^C[GA][CT]GT
1878 ACACGT
AlwI 803 GATCC GGATC(4/5)
802 GGATC
AspHI 1676 GAGCTC G[AT]GC[AT]^C
2227 GTGCTC
BbsI 120 GAAGAC GAAGAC(2/6)
648 GAAGAC
BglI 1061 GCCCTCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1098 GCCTCCTGGGC
BpuAI 120 GAAGAC GAAGAC(2/6)
648 GAAGAC
BsaWI 984 TCCGGA [AT]^CCGG[AT]
1771 ACCGGA
BsgI 2179 GTGCAG GTGCAG(16/14)
2377 GTGCAG
BsiHKAI 1676 GAGCTC G[AT]GC[AT]^C
2227 GTGCTC
BspMI 2117 ACCTGC ACCTGC(4/8)
1868 GCAGGT
BstXI 1352 CCACTCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2236 CCAGCAGTCTGG
Cac8I 1515 GCGGGC GC[ACGT]^[ACGT]GC
1798 GCTGGC
DpnII 667 GATC ^GATC
803 GATC
EcoRI 95 GAATTC G^AATTC
1942 GAATTC
HpaI 168 GTTAAC GTT^AAC
726 GTTAAC
MboI 667 GATC ^GATC
803 GATC
MspA1I 1040 CAGCTG C[AC]G^C[GT]G
1835 CAGCTG
NdeII 667 GATC ^GATC
803 GATC
PpuMI 1077 AGGTCCC [GA]G^G[AT]CC[CT]
1653 GGGACCC
PvuII 1040 CAGCTG CAG^CTG
1835 CAGCTG
Sau3AI 667 GATC ^GATC
803 GATC
SfiI 1060 GGCCCTCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1097 GGCCTCCTGGGCC
StuI 32 AGGCCT AGG^CCT
733 AGGCCT
Tsp45I 2209 GTCAC ^GT[GC]AC
2414 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmFI BsmI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
CfoI Cfr13I DdeI DraII EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeII
MaeIII MboII MnlI MseI MspI
MvaI MwoI NciI NlaIII NlaIV
NspI PleI RsaI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XcmI