Restriction map for pF1KSDB0061
##################################
# NO CUT ENZYMES (   76 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AgeI        
AocI        AscI        AseI        AsnI        Asp718I     
AspI        AvrII       BciVI       BclI        BfaI        
BfrI        BlnI        BsaBI       BsiWI       BsmI        
Bsp1407I    BspDI       BsrGI       BssHII      Bst1107I    
BstBI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DrdI        EagI        EclXI       Eco52I      
Eco81I      EcoT22I     FbaI        FseI        HpaI        
KpnI        MaeI        MamI        MfeI        MluI        
MseI        MunI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SalI        SbfI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        Tru9I       Tth111I     
XbaI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AatII        1534  GACGTC               GACGT^C
AhdI         3120  GACACATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1728  GTGCAC               G^TGCAC
ApaLI        1728  GTGCAC               G^TGCAC
Asp700I       114  GAAGCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        3120  GACACATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI         791  GGATCC               G^GATCC
BbrPI        2119  CACGTG               CAC^GTG
BbvCI        2469  CCTCAGC              CCTCAGC(-5/-2)
BsgI         1279  GTGCAG               GTGCAG(16/14)
BspLU11I      275  ACATGT               A^CATGT
BstAPI       1440  GCAGAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2301  GGTCACC              G^GT[ACGT]ACC
BstPI        2301  GGTCACC              G^GT[ACGT]ACC
Eam1105I     3120  GACACATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      2301  GGTCACC              G^GT[ACGT]ACC
EcoRI        3016  GAATTC               G^AATTC
EcoRV         424  GATATC               GAT^ATC
HincII       1978  GTCAAC               GT[CT]^[GA]AC
HindII       1978  GTCAAC               GT[CT]^[GA]AC
HindIII      2156  AAGCTT               A^AGCTT
PaeR7I        451  CTCGAG               C^TCGAG
PmaCI        2119  CACGTG               CAC^GTG
PmlI         2119  CACGTG               CAC^GTG
SacI         2763  GAGCTC               GAGCT^C
SapI         1626  GCTCTTC              GCTCTTC(1/4)
SfiI         3253  GGCCAGGAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI         3050  GCATGC               GCATG^C
XhoI          451  CTCGAG               C^TCGAG
XmnI          114  GAAGCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcsI         1885  AAATTC               [GA]^AATT[CT]
             3016  GAATTC              
AflIII        275  ACATGT               A^C[GA][CT]GT
             1388  ACGTGT              
ApoI         1885  AAATTC               [GA]^AATT[CT]
             3016  GAATTC              
AviII         479  TGCGCA               TGC^GCA
             1847  TGCGCA              
BbeI         1661  GGCGCC               GGCGC^C
             2114  GGCGCC              
BglII         557  AGATCT               A^GATCT
             1172  AGATCT              
BlpI         1353  GCTGAGC              GC^T[ACGT]AGC
             3177  GCTGAGC             
Bpu1102I     1353  GCTGAGC              GC^T[ACGT]AGC
             3177  GCTGAGC             
BsaI         1765  GAGACC               GGTCTC(1/5)
             1524  GGTCTC              
BsmBI        1562  GAGACG               CGTCTC(1/5)
             1918  GAGACG              
BspHI         680  TCATGA               T^CATGA
             1370  TCATGA              
BsrDI          96  CATTGC               GCAATG(2/0)
             2864  GCAATG              
CelII        1353  GCTGAGC              GC^T[ACGT]AGC
             3177  GCTGAGC             
DraIII         34  CACAACGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1807  CACCAGGTG           
FspI          479  TGCGCA               TGC^GCA
             1847  TGCGCA              
KasI         1661  GGCGCC               G^GCGCC
             2114  GGCGCC              
KspI         1927  CCGCGG               CCGC^GG
             2685  CCGCGG              
NarI         1661  GGCGCC               GG^CGCC
             2114  GGCGCC              
PpuMI        1462  GGGACCC              [GA]G^G[AT]CC[CT]
             2397  GGGACCT             
RcaI          680  TCATGA               T^CATGA
             1370  TCATGA              
SacII        1927  CCGCGG               CCGC^GG
             2685  CCGCGG              
ScaI          509  AGTACT               AGT^ACT
             1310  AGTACT              
SfoI         1661  GGCGCC               GGC^GCC
             2114  GGCGCC              
SmlI          451  CTCGAG               C^T[CT][GA]AG
             2710  CTCAAG              
TfiI          243  GAATC                G^A[AT]TC
             2296  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (  127 enzymes) #
#########################################

AccII       AccIII      AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       Aor51HI     ApaI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvI        BcgI        BcnI        
BglI        BmyI        BpmI        BpuAI       BsaAI       
BsaHI       BsaJI       BsaWI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspEI       BspMI       
BsrBI       BsrFI       BsrI        BssKI       BssSI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco47III    EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MroI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PflMI       PleI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SexAI       SfaNI       SfcI        SmaI        StuI        
StyI        TaqI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI        
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