Restriction map for pF1KSDB0061
 (Linear) (Six-base) MAPSORT of: KIBB0061.seq  Check: 3602  from: 1  to: 3294



KIBB0061.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1973    3294 
   Size:       1973    1321
 
AatII G_ACGT'C

Cuts at:      0    1538    3294 
   Size:       1538    1756
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1276    1849    2595    3294 
   Size:       1276     573     746     699

  Fragments arranged by size: 

                1276     746     699     573
 
AfeI AGC'GCT

Cuts at:      0      16     118    2572    3294 
   Size:         16     102    2454     722

  Fragments arranged by size: 

                2454     722     102      16
 
AflIII A'CryG_T

Cuts at:      0     275    1388    3294 
   Size:        275    1113    1906

  Fragments arranged by size: 

                1906    1113     275
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3125    3294 
   Size:       3125     169
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1165    1916    2887    3294 
   Size:       1165     751     971     407

  Fragments arranged by size: 

                1165     971     751     407
 
AlwNI CAG_nnn'CTG

Cuts at:      0     639     672     813     863     986    1434    1446    1965 
   Size:        639      33     141      50     123     448      12     519

Cuts at:   1965    2265    2454    3088    3294 
   Size:        300     189     634     206

  Fragments arranged by size: 

                 639     634     519     448     300     206     189     141
                 123      50      33      12
 
ApaI G_GGCC'C

Cuts at:      0    2896    2950    3144    3294 
   Size:       2896      54     194     150

  Fragments arranged by size: 

                2896     194     150      54
 
ApaLI G'TGCA_C

Cuts at:      0    1728    3294 
   Size:       1728    1566
 
ApoI r'AATT_y

Cuts at:      0    1885    3016    3294 
   Size:       1885    1131     278

  Fragments arranged by size: 

                1885    1131     278
 
AvaI C'yCGr_G

Cuts at:      0     451     884    1633    1778    1915    2248    2828    2942 
   Size:        451     433     749     145     137     333     580     114

Cuts at:   2942    2984    3225    3294 
   Size:         42     241      69

  Fragments arranged by size: 

                 749     580     451     433     333     241     145     137
                 114      69      42
 
BamHI G'GATC_C

Cuts at:      0     791    3294 
   Size:        791    2503
 
BanI G'GyrC_C

Cuts at:      0    1661    2114    2662    2871    2926    3134    3294 
   Size:       1661     453     548     209      55     208     160

  Fragments arranged by size: 

                1661     548     453     209     208     160      55
 
BanII G_rGCy'C

Cuts at:      0    1070    1360    1991    2767    2896    2950    3144    3294 
   Size:       1070     290     631     776     129      54     194     150

  Fragments arranged by size: 

                1070     776     631     290     194     150     129      54
            
 
BbeI G_GCGC'C

Cuts at:      0    1665    2118    3294 
   Size:       1665     453    1176

  Fragments arranged by size: 

                1665    1176     453
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      95     352     875    2529    3294 
   Size:         95     257     523    1654     765

  Fragments arranged by size: 

                1654     765     523     257      95
 
BbvCI CC'TCA_GC

Cuts at:      0    2470    3294 
   Size:       2470     824
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1194    1228    1951    1985    2068    2102    2556    2590 
   Size:       1194      34     723      34      83      34     454      34

Cuts at:   2590    3294 
   Size:        704

  Fragments arranged by size: 

                1194     723     704     454      83      34      34      34
                  34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     503     989    1364    1932    3260    3294 
   Size:        503     486     375     568    1328      34

  Fragments arranged by size: 

                1328     568     503     486     375      34
 
BglII A'GATC_T

Cuts at:      0     557    1172    3294 
   Size:        557     615    2122

  Fragments arranged by size: 

                2122     615     557
 
BlpI GC'TnA_GC

Cuts at:      0    1354    3178    3294 
   Size:       1354    1824     116

  Fragments arranged by size: 

                1824    1354     116
 
Bme1580I G_kGCm'C

Cuts at:      0    1732    2876    2896    2929    2950    2991    3144    3294 
   Size:       1732    1144      20      33      21      41     153     150

  Fragments arranged by size: 

                1732    1144     153     150      41      33      21      20
            
 
BmgBI CAC'GTC

Cuts at:      0     417    1389    1922    2992    3294 
   Size:        417     972     533    1070     302

  Fragments arranged by size: 

                1070     972     533     417     302
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     228    1794    3294 
   Size:        228    1566    1500

  Fragments arranged by size: 

                1566    1500     228
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1585    2485    3294 
   Size:       1585     900     809

  Fragments arranged by size: 

                1585     900     809
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     595    1506    2074    2257    2548    2926    2939    3294 
   Size:        595     911     568     183     291     378      13     355

  Fragments arranged by size: 

                 911     595     568     378     355     291     183      13
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    2470    3294 
   Size:       2470     824
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2695    3294 
   Size:       2695     599
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1530    1759    3294 
   Size:       1530     229    1535

  Fragments arranged by size: 

                1535    1530     229
 
BsaAI yAC'GTr

Cuts at:      0    2121    3156    3282    3294 
   Size:       2121    1035     126      12

  Fragments arranged by size: 

                2121    1035     126      12
 
BsaHI Gr'CG_yC

Cuts at:      0      92    1535    1662    1955    2115    2353    3294 
   Size:         92    1443     127     293     160     238     941

  Fragments arranged by size: 

                1443     941     293     238     160     127      92
 
BsaWI w'CCGG_w

Cuts at:      0     110     281     687    1619    2129    3294 
   Size:        110     171     406     932     510    1165

  Fragments arranged by size: 

                1165     932     510     406     171     110
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2899    2929    3294 
   Size:       2899      30     365

  Fragments arranged by size: 

                2899     365      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1285    1804    2071    2155    2254    2593    3294 
   Size:       1285     519     267      84      99     339     701

  Fragments arranged by size: 

                1285     701     519     339     267      99      84
 
BseYI C'CCAG_C

Cuts at:      0     340     799     927     986    1666    2648    3110    3294 
   Size:        340     459     128      59     680     982     462     184

  Fragments arranged by size: 

                 982     680     462     459     340     184     128      59
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1300    3294 
   Size:       1300    1994
 
BsiEI CG_ry'CG

Cuts at:      0     204    2316    2724    3294 
   Size:        204    2112     408     570

  Fragments arranged by size: 

                2112     570     408     204
 
BsiHKAI G_wGCw'C

Cuts at:      0     161    1732    2014    2711    2767    3008    3294 
   Size:        161    1571     282     697      56     241     286

  Fragments arranged by size: 

                1571     697     286     282     241     161      56
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1556    1912    3294 
   Size:       1556     356    1382

  Fragments arranged by size: 

                1556    1382     356
 
Bsp1286I G_dGCh'C

Cuts at:      0     161    1070    1360    1732    1991    2014    2711    2767 
   Size:        161     909     290     372     259      23     697      56

Cuts at:   2767    2876    2896    2929    2950    2991    3008    3144    3294 
   Size:        109      20      33      21      41      17     136     150

  Fragments arranged by size: 

                 909     697     372     290     259     161     150     136
                 109      56      41      33      23      21      20      17
            
 
BspEI T'CCGG_A

Cuts at:      0     110    1619    2129    3294 
   Size:        110    1509     510    1165

  Fragments arranged by size: 

                1509    1165     510     110
 
BspHI T'CATG_A

Cuts at:      0     680    1370    3294 
   Size:        680     690    1924

  Fragments arranged by size: 

                1924     690     680
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     771     932    1973    3294 
   Size:        771     161    1041    1321

  Fragments arranged by size: 

                1321    1041     771     161
 
BsrBI CCG'CTC

Cuts at:      0      50    2187    2244    2280    3294 
   Size:         50    2137      57      36    1014

  Fragments arranged by size: 

                2137    1014      57      50      36
 
BsrDI GCAATG_nn'

Cuts at:      0      95    2871    3294 
   Size:         95    2776     423

  Fragments arranged by size: 

                2776     423      95
 
BsrFI r'CCGG_y

Cuts at:      0    1492    1675    1855    3046    3242    3294 
   Size:       1492     183     180    1191     196      52

  Fragments arranged by size: 

                1492    1191     196     183     180      52
 
BssSI C'ACGA_G

Cuts at:      0     826    1093    1468    3294 
   Size:        826     267     375    1826

  Fragments arranged by size: 

                1826     826     375     267
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1446    3294 
   Size:       1446    1848
 
BstEII G'GTnAC_C

Cuts at:      0    2301    3294 
   Size:       2301     993
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1186    1516    1707    2856    2964    3294 
   Size:       1186     330     191    1149     108     330

  Fragments arranged by size: 

                1186    1149     330     330     191     108
 
BstYI r'GATC_y

Cuts at:      0     518     557     791     836    1172    3206    3219    3294 
   Size:        518      39     234      45     336    2034      13      75

  Fragments arranged by size: 

                2034     518     336     234      75      45      39      13
            
 
BtgI C'CryG_G

Cuts at:      0    1409    1927    2685    3294 
   Size:       1409     518     758     609

  Fragments arranged by size: 

                1409     758     609     518
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2762    3294 
   Size:       2762     532
 
BtsI GCAGTG_nn'

Cuts at:      0    1432    2711    3294 
   Size:       1432    1279     583

  Fragments arranged by size: 

                1432    1279     583
 
DraIII CAC_nnn'GTG

Cuts at:      0      39    1812    3294 
   Size:         39    1773    1482

  Fragments arranged by size: 

                1773    1482      39
 
EaeI y'GGCC_r

Cuts at:      0    1058    1712    1924    2789    3044    3079    3252    3294 
   Size:       1058     654     212     865     255      35     173      42

  Fragments arranged by size: 

                1058     865     654     255     212     173      42      35
            
 
EarI CTCTTCn'nnn_

Cuts at:      0    1087    1127    1576    1633    3294 
   Size:       1087      40     449      57    1661

  Fragments arranged by size: 

                1661    1087     449      57      40
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     410    2984    3294 
   Size:        410    2574     310

  Fragments arranged by size: 

                2574     410     310
 
EcoICRI GAG'CTC

Cuts at:      0    2765    3294 
   Size:       2765     529
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     595    1276    1506    1849    2074    2257    2548    2595 
   Size:        595     681     230     343     225     183     291      47

Cuts at:   2595    2926    2939    3294 
   Size:        331      13     355

  Fragments arranged by size: 

                 681     595     355     343     331     291     230     225
                 183      47      13
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     220    1060    1096    2053    2056    2503    3294 
   Size:        220     840      36     957       3     447     791

  Fragments arranged by size: 

                 957     840     791     447     220      36       3
 
EcoO109I rG'GnC_Cy

Cuts at:      0     647    1463    1775    2398    2594    2892    2893    2946 
   Size:        647     816     312     623     196     298       1      53

Cuts at:   2946    2947    3141    3261    3294 
   Size:          1     194     120      33

  Fragments arranged by size: 

                 816     647     623     312     298     196     194     120
                  53      33       1       1
 
EcoRI G'AATT_C

Cuts at:      0    3016    3294 
   Size:       3016     278
 
EcoRV GAT'ATC

Cuts at:      0     426    3294 
   Size:        426    2868
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      33    2358    3294 
   Size:         33    2325     936

  Fragments arranged by size: 

                2325     936      33
 
FspI TGC'GCA

Cuts at:      0     481    1849    3294 
   Size:        481    1368    1445

  Fragments arranged by size: 

                1445    1368     481
 
HaeII r_GCGC'y

Cuts at:      0      18     120    1493    1665    2118    2574    3294 
   Size:         18     102    1373     172     453     456     720

  Fragments arranged by size: 

                1373     720     456     453     172     102      18
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2899    2931    3111    3143    3294 
   Size:       2899      32     180      32     151

  Fragments arranged by size: 

                2899     180     151      32      32
 
HincII GTy'rAC

Cuts at:      0    1980    3294 
   Size:       1980    1314
 
HindIII A'AGCT_T

Cuts at:      0    2156    3294 
   Size:       2156    1138
 
KasI G'GCGC_C

Cuts at:      0    1661    2114    3294 
   Size:       1661     453    1180

  Fragments arranged by size: 

                1661    1180     453
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     464     587    1601    2186    2704    3294 
   Size:        464     123    1014     585     518     590

  Fragments arranged by size: 

                1014     590     585     518     464     123
 
MscI TGG'CCA

Cuts at:      0    1060    1714    2791    3294 
   Size:       1060     654    1077     503

  Fragments arranged by size: 

                1077    1060     654     503
 
MslI CAynn'nnrTG

Cuts at:      0    1012    1147    1720    1843    3294 
   Size:       1012     135     573     123    1451

  Fragments arranged by size: 

                1451    1012     573     135     123
 
MspA1I CmG'CkG

Cuts at:      0     447     636     813     941     986    1295    1484    1541 
   Size:        447     189     177     128      45     309     189      57

Cuts at:   1541    1748    1929    2687    3294 
   Size:        207     181     758     607

  Fragments arranged by size: 

                 758     607     447     309     207     189     189     181
                 177     128      57      45
 
NaeI GCC'GGC

Cuts at:      0    1494    1677    1857    3048    3244    3294 
   Size:       1494     183     180    1191     196      50

  Fragments arranged by size: 

                1494    1191     196     183     180      50
 
NarI GG'CG_CC

Cuts at:      0    1662    2115    3294 
   Size:       1662     453    1179

  Fragments arranged by size: 

                1662    1179     453
 
NgoMIV G'CCGG_C

Cuts at:      0    1492    1675    1855    3046    3242    3294 
   Size:       1492     183     180    1191     196      52

  Fragments arranged by size: 

                1492    1191     196     183     180      52
 
NspI r_CATG'y

Cuts at:      0     279     291    3054    3294 
   Size:        279      12    2763     240

  Fragments arranged by size: 

                2763     279     240      12
 
PasI CC'CwG_GG

Cuts at:      0    2964    3294 
   Size:       2964     330
 
PciI A'CATG_T

Cuts at:      0     275    3294 
   Size:        275    3019
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     639     813    3042    3088    3156    3168    3294 
   Size:        639     174    2229      46      68      12     126

  Fragments arranged by size: 

                2229     639     174     126      68      46      12
 
PfoI T'CCnGG_A

Cuts at:      0    2051    2525    3294 
   Size:       2051     474     769

  Fragments arranged by size: 

                2051     769     474
 
PmlI CAC'GTG

Cuts at:      0    2121    3294 
   Size:       2121    1173
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     508     540    2059    2091    3294 
   Size:        508      32    1519      32    1203

  Fragments arranged by size: 

                1519    1203     508      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1463    2398    3294 
   Size:       1463     935     896

  Fragments arranged by size: 

                1463     935     896
 
PspOMI G'GGCC_C

Cuts at:      0    2892    2946    3140    3294 
   Size:       2892      54     194     154

  Fragments arranged by size: 

                2892     194     154      54
 
PspXI vC'TCGA_Gb

Cuts at:      0     451    3294 
   Size:        451    2843
 
PstI C_TGCA'G

Cuts at:      0     437     824     953    1232    1442    1451    1895    3294 
   Size:        437     387     129     279     210       9     444    1399

  Fragments arranged by size: 

                1399     444     437     387     279     210     129       9
            
 
PvuII CAG'CTG

Cuts at:      0     636     813    1484    3294 
   Size:        636     177     671    1810

  Fragments arranged by size: 

                1810     671     636     177
 
SacI G_AGCT'C

Cuts at:      0    2767    3294 
   Size:       2767     527
 
SacII CC_GC'GG

Cuts at:      0    1930    2688    3294 
   Size:       1930     758     606

  Fragments arranged by size: 

                1930     758     606
 
SanDI GG'GwC_CC

Cuts at:      0    1463    3294 
   Size:       1463    1831
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1633    3294 
   Size:       1633    1661
 
ScaI AGT'ACT

Cuts at:      0     511    1312    3294 
   Size:        511     801    1982

  Fragments arranged by size: 

                1982     801     511
 
SexAI A'CCwGG_T

Cuts at:      0     725    1706    1808    3294 
   Size:        725     981     102    1486

  Fragments arranged by size: 

                1486     981     725     102
 
SfcI C'TryA_G

Cuts at:      0     433     820     949    1228    1438    1447    1891    2623 
   Size:        433     387     129     279     210       9     444     732

Cuts at:   2623    3294 
   Size:        671

  Fragments arranged by size: 

                 732     671     444     433     387     279     210     129
                   9
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    3260    3294 
   Size:       3260      34
 
SfoI GGC'GCC

Cuts at:      0    1663    2116    3294 
   Size:       1663     453    1178

  Fragments arranged by size: 

                1663    1178     453
 
SmaI CCC'GGG

Cuts at:      0    2830    2944    2986    3294 
   Size:       2830     114      42     308

  Fragments arranged by size: 

                2830     308     114      42
 
SmlI C'TyrA_G

Cuts at:      0     451    2710    3294 
   Size:        451    2259     584

  Fragments arranged by size: 

                2259     584     451
 
SphI G_CATG'C

Cuts at:      0    3054    3294 
   Size:       3054     240
 
StuI AGG'CCT

Cuts at:      0     457    1612    2107    2937    3294 
   Size:        457    1155     495     830     357

  Fragments arranged by size: 

                1155     830     495     457     357
 
StyI C'CwwG_G

Cuts at:      0     383    1061    2015    2792    3294 
   Size:        383     678     954     777     502

  Fragments arranged by size: 

                 954     777     678     502     383
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2289    2560    2944    3294 
   Size:       2289     271     384     350

  Fragments arranged by size: 

                2289     384     350     271
 
TatI w'GTAC_w

Cuts at:      0     509    1136    1310    3294 
   Size:        509     627     174    1984

  Fragments arranged by size: 

                1984     627     509     174
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1375    1407    3266    3294 
   Size:       1375      32    1859      28

  Fragments arranged by size: 

                1859    1375      32      28
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     366     570     972    1513    1707    3294 
   Size:        366     204     402     541     194    1587

  Fragments arranged by size: 

                1587     541     402     366     204     194
 
XhoI C'TCGA_G

Cuts at:      0     451    3294 
   Size:        451    2843
 
XmaI C'CCGG_G

Cuts at:      0    2828    2942    2984    3294 
   Size:       2828     114      42     310

  Fragments arranged by size: 

                2828     310     114      42
 
XmnI GAAnn'nnTTC

Cuts at:      0     118    3294 
   Size:        118    3176
 
ZraI GAC'GTC

Cuts at:      0    1536    3294 
   Size:       1536    1758
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI   AflIII     AhdI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI   DraIII
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI
     PasI     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI   PspOMI
    PspXI     PstI    PvuII     SacI    SacII    SanDI     SapI     ScaI
    SexAI     SfcI     SfiI     SfoI     SmaI     SmlI     SphI     StuI
     StyI    TaqII     TatI     TstI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII     AgeI     AleI     AloI     AscI
     AseI    AsiSI    AvrII     BaeI    BciVI     BclI    BfrBI     BmtI
    BsaBI    BsiWI     BsmI    BsrGI   BssHII    BstBI  BstZ17I   Bsu36I
     ClaI    CspCI     DraI     DrdI     EagI     FseI    FspAI     HpaI
     KpnI     MfeI     MluI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PmeI    PshAI     PsiI     PsrI     PvuI    RsrII
     SalI     SbfI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI
     SwaI  Tth111I     XbaI
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