Restriction map for pF1KSDB0059
##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Aor51HI     AscI        
AseI        AsnI        Asp718I     AspEI       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BciVI       
BclI        BfrI        BlnI        BsaAI       BseAI       
BsiEI       BsiWI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrGI       BssHII      BstBI       ClaI        
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoRV       EcoT22I     FbaI        
FseI        HincII      HindII      HpaI        KasI        
KpnI        KspI        MfeI        MluI        MroI        
MseI        MunI        NaeI        NarI        NdeI        
NgoMIV      NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tru9I       XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

AccI         3070  GTATAC               GT^[AC][GT]AC
AcsI         1984  GAATTC               [GA]^AATT[CT]
Alw44I        419  GTGCAC               G^TGCAC
ApaLI         419  GTGCAC               G^TGCAC
ApoI         1984  GAATTC               [GA]^AATT[CT]
Asp700I      1227  GAAAGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BfaI         3154  CTAG                 C^TAG
BglII        1922  AGATCT               A^GATCT
BsaBI        3385  GATGGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1598  ACCGGA               [AT]^CCGG[AT]
BsmI         2223  GAATGC               GAATGC(1/-1)
BspHI        2598  TCATGA               T^CATGA
BssSI        2390  CACGAG               CACGAG(-5/-1)
Bst1107I     3070  GTATAC               GTA^TAC
BstZ17I      3070  GTATAC               GTA^TAC
DrdI         1510  GACCTAAGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         888  CCTGCGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1984  GAATTC               G^AATTC
HindIII      1753  AAGCTT               A^AGCTT
MaeI         3154  CTAG                 C^TAG
MamI         3385  GATGGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI         3153  GCTAGC               G^CTAGC
PstI         1939  CTGCAG               CTGCA^G
RcaI         2598  TCATGA               T^CATGA
ScaI         2960  AGTACT               AGT^ACT
XmnI         1227  GAAAGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AcyI          557  GACGCC               G[GA]^CG[CT]C
             1717  GACGCC              
AocI         1512  CCTAAGG              CC^T[ACGT]AGG
             3297  CCTGAGG             
AspI         1702  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1773  GACCTTGTC           
AviII         125  TGCGCA               TGC^GCA
              890  TGCGCA              
BcgI          146  GCAGCCTCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              249  GCAGGCGAATCG        
BlpI          495  GCTCAGC              GC^T[ACGT]AGC
              834  GCTGAGC             
Bpu1102I      495  GCTCAGC              GC^T[ACGT]AGC
              834  GCTGAGC             
BsaHI         557  GACGCC               G[GA]^CG[CT]C
             1717  GACGCC              
BsaI         2320  GAGACC               GGTCTC(1/5)
             1440  GGTCTC              
BsrBI        2497  GAGCGG               CCGCTC(-3/-3)
             2776  GAGCGG              
BsrDI        1533  CATTGC               GCAATG(2/0)
             2424  CATTGC              
BsrFI         176  ACCGGC               [GA]^CCGG[CT]
              743  ACCGGC              
BstAPI       1198  GCAGTGGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2367  GCAGGAGCTGC         
BstEII       2070  GGTCACC              G^GT[ACGT]ACC
             2277  GGTCACC             
BstPI        2070  GGTCACC              G^GT[ACGT]ACC
             2277  GGTCACC             
Bsu36I       1512  CCTAAGG              CC^T[ACGT]AGG
             3297  CCTGAGG             
CelII         495  GCTCAGC              GC^T[ACGT]AGC
              834  GCTGAGC             
Cfr10I        176  ACCGGC               [GA]^CCGG[CT]
              743  ACCGGC              
DraIII       1677  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2033  CACTGAGTG           
Eco81I       1512  CCTAAGG              CC^T[ACGT]AGG
             3297  CCTGAGG             
EcoO65I      2070  GGTCACC              G^GT[ACGT]ACC
             2277  GGTCACC             
FspI          125  TGCGCA               TGC^GCA
              890  TGCGCA              
Hin1I         557  GACGCC               G[GA]^CG[CT]C
             1717  GACGCC              
MaeII         110  ACGT                 A^CGT
             1630  ACGT                
NspI         2792  GCATGT               [GA]CATG^[CT]
             2919  GCATGT              
PflFI        1702  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1773  GACCTTGTC           
PpuMI         503  AGGACCT              [GA]G^G[AT]CC[CT]
             1166  GGGACCT             
SmaI         1967  CCCGGG               CCC^GGG
             2852  CCCGGG              
StuI          267  AGGCCT               AGG^CCT
             1667  AGGCCT              
Tth111I      1702  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1773  GACCTTGTC           
XmaCI        1967  CCCGGG               C^CCGGG
             2852  CCCGGG              
XmaI         1967  CCCGGG               C^CCGGG
             2852  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  104 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       ApaI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvI        
BcnI        BglI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PvuII       RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TfiI        TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII       
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