Restriction map for pF1KSDB0059
 (Linear) (Six-base) MAPSORT of: KIBB0059.seq  Check: 1657  from: 1  to: 3411



KIBB0059.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:19  ..

AccI GT'mk_AC

Cuts at:      0    3071    3411 
   Size:       3071     340
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1389    1708    1918    3411 
   Size:       1389     319     210    1493

  Fragments arranged by size: 

                1493    1389     319     210
 
AleI CACnn'nnGTG

Cuts at:      0     872    1396    3411 
   Size:        872     524    2015

  Fragments arranged by size: 

                2015     872     524
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     365     397     529     561    3411 
   Size:        365      32     132      32    2850

  Fragments arranged by size: 

                2850     365     132      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     231     459     507    1284    1830    2139    2373    2386 
   Size:        231     228      48     777     546     309     234      13

Cuts at:   2386    3411 
   Size:       1025

  Fragments arranged by size: 

                1025     777     546     309     234     231     228      48
                  13
 
ApaI G_GGCC'C

Cuts at:      0     648    2184    3334    3382    3411 
   Size:        648    1536    1150      48      29

  Fragments arranged by size: 

                1536    1150     648      48      29
 
ApaLI G'TGCA_C

Cuts at:      0     419    3411 
   Size:        419    2992
 
ApoI r'AATT_y

Cuts at:      0    1984    3411 
   Size:       1984    1427
 
AvaI C'yCGr_G

Cuts at:      0     804     919    1671    1967    2852    3211    3251    3370 
   Size:        804     115     752     296     885     359      40     119

Cuts at:   3370    3411 
   Size:         41

  Fragments arranged by size: 

                 885     804     752     359     296     119     115      41
                  40
 
BanI G'GyrC_C

Cuts at:      0     118     915    1018    1204    1675    2269    3289    3403 
   Size:        118     797     103     186     471     594    1020     114

Cuts at:   3403    3411 
   Size:          8

  Fragments arranged by size: 

                1020     797     594     471     186     118     114     103
                   8
 
BanII G_rGCy'C

Cuts at:      0      12     648     855    1381    2184    2397    2572    2789 
   Size:         12     636     207     526     803     213     175     217

Cuts at:   2789    3334    3382    3411 
   Size:        545      48      29

  Fragments arranged by size: 

                 803     636     545     526     217     213     207     175
                  48      29      12
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1224    1922    2394    3263    3289    3411 
   Size:       1224     698     472     869      26     122

  Fragments arranged by size: 

                1224     869     698     472     122      26
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     135     169     238     272    3411 
   Size:        135      34      69      34    3139

  Fragments arranged by size: 

                3139     135      69      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     155     566     596    3411 
   Size:        155     411      30    2815

  Fragments arranged by size: 

                2815     411     155      30
 
BglII A'GATC_T

Cuts at:      0    1922    3411 
   Size:       1922    1489
 
BlpI GC'TnA_GC

Cuts at:      0     496     835    3411 
   Size:        496     339    2576

  Fragments arranged by size: 

                2576     496     339
 
Bme1580I G_kGCm'C

Cuts at:      0     423     601     648     920    1023    1209    1678    2184 
   Size:        423     178      47     272     103     186     469     506

Cuts at:   2184    2274    2860    3110    3227    3292    3334    3382    3411 
   Size:         90     586     250     117      65      42      48      29

  Fragments arranged by size: 

                 586     506     469     423     272     250     186     178
                 117     103      90      65      48      47      42      29
            
 
BmgBI CAC'GTC

Cuts at:      0     111    3411 
   Size:        111    3300
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1101    3055    3222    3318    3411 
   Size:       1101    1954     167      96      93

  Fragments arranged by size: 

                1954    1101     167      96      93
 
BmtI G_CTAG'C

Cuts at:      0    3157    3411 
   Size:       3157     254
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2244    2979    3411 
   Size:       2244     735     432

  Fragments arranged by size: 

                2244     735     432
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     284     313     481     869    1306    1489    1717    1774 
   Size:        284      29     168     388     437     183     228      57

Cuts at:   1774    1820    2080    2353    2668    2767    2974    3411 
   Size:         46     260     273     315      99     207     437

  Fragments arranged by size: 

                 437     437     388     315     284     273     260     228
                 207     183     168      99      57      46      29
 
Bpu10I CC'TnA_GC

Cuts at:      0    1636    2296    2756    3411 
   Size:       1636     660     460     655

  Fragments arranged by size: 

                1636     660     655     460
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1446    2314    3411 
   Size:       1446     868    1097

  Fragments arranged by size: 

                1446    1097     868
 
BsaBI GATnn'nnATC

Cuts at:      0    3389    3411 
   Size:       3389      22
 
BsaHI Gr'CG_yC

Cuts at:      0     558    1718    3411 
   Size:        558    1160    1693

  Fragments arranged by size: 

                1693    1160     558
 
BsaWI w'CCGG_w

Cuts at:      0    1598    3411 
   Size:       1598    1813
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     349     365     379     395    1406    1436    3411 
   Size:        349      16      14      16    1011      30    1975

  Fragments arranged by size: 

                1975    1011     349      30      16      16      14
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     310     625    2971    3292    3411 
   Size:        310     315    2346     321     119

  Fragments arranged by size: 

                2346     321     315     310     119
 
BseYI C'CCAG_C

Cuts at:      0    1050    1096    1543    1867    1874    2091    2096    2132 
   Size:       1050      46     447     324       7     217       5      36

Cuts at:   2132    2247    2444    2788    3139    3375    3411 
   Size:        115     197     344     351     236      36

  Fragments arranged by size: 

                1050     447     351     344     324     236     217     197
                 115      46      36      36       7       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     845    2146    2372    3411 
   Size:        157     688    1301     226    1039

  Fragments arranged by size: 

                1301    1039     688     226     157
 
BsiHKAI G_wGCw'C

Cuts at:      0     222     423     497    1403    3411 
   Size:        222     201      74     906    2008

  Fragments arranged by size: 

                2008     906     222     201      74
 
BsmI GAATG_Cn'

Cuts at:      0    2229    3411 
   Size:       2229    1182
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     457    1709    2938    3411 
   Size:        457    1252    1229     473

  Fragments arranged by size: 

                1252    1229     473     457
 
Bsp1286I G_dGCh'C

Cuts at:      0      12     222     423     497     601     648     855     920 
   Size:         12     210     201      74     104      47     207      65

Cuts at:    920    1023    1209    1381    1403    1678    2184    2274    2397 
   Size:        103     186     172      22     275     506      90     123

Cuts at:   2397    2572    2789    2860    3110    3227    3292    3334    3382 
   Size:        175     217      71     250     117      65      42      48

Cuts at:   3382    3411 
   Size:         29

  Fragments arranged by size: 

                 506     275     250     217     210     207     201     186
                 175     172     123     117     104     103      90      74
                  71      65      65      48      47      42      29      22
                  12
 
BspHI T'CATG_A

Cuts at:      0    2598    3411 
   Size:       2598     813
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1376    2087    2628    3411 
   Size:       1376     711     541     783

  Fragments arranged by size: 

                1376     783     711     541
 
BsrBI CCG'CTC

Cuts at:      0    2499    2778    3411 
   Size:       2499     279     633

  Fragments arranged by size: 

                2499     633     279
 
BsrDI GCAATG_nn'

Cuts at:      0    1532    2423    3411 
   Size:       1532     891     988

  Fragments arranged by size: 

                1532     988     891
 
BsrFI r'CCGG_y

Cuts at:      0     176     743    3411 
   Size:        176     567    2668

  Fragments arranged by size: 

                2668     567     176
 
BssSI C'ACGA_G

Cuts at:      0    2390    3411 
   Size:       2390    1021
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1204    2373    3411 
   Size:       1204    1169    1038

  Fragments arranged by size: 

                1204    1169    1038
 
BstEII G'GTnAC_C

Cuts at:      0    2070    2277    3411 
   Size:       2070     207    1134

  Fragments arranged by size: 

                2070    1134     207
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     397     912    2007    2110    2331    2871    3090    3411 
   Size:        397     515    1095     103     221     540     219     321

  Fragments arranged by size: 

                1095     540     515     397     321     221     219     103
            
 
BstYI r'GATC_y

Cuts at:      0     455    1100    1886    1922    3411 
   Size:        455     645     786      36    1489

  Fragments arranged by size: 

                1489     786     645     455      36
 
BstZ17I GTA'TAC

Cuts at:      0    3072    3411 
   Size:       3072     339
 
Bsu36I CC'TnA_GG

Cuts at:      0    1513    3298    3411 
   Size:       1513    1785     113

  Fragments arranged by size: 

                1785    1513     113
 
BtgI C'CryG_G

Cuts at:      0    1242    2000    2324    3083    3411 
   Size:       1242     758     324     759     328

  Fragments arranged by size: 

                1242     759     758     328     324
 
BtsI GCAGTG_nn'

Cuts at:      0    1205    1403    2158    3157    3411 
   Size:       1205     198     755     999     254

  Fragments arranged by size: 

                1205     999     755     254     198
 
DraIII CAC_nnn'GTG

Cuts at:      0    1682    2038    3411 
   Size:       1682     356    1373

  Fragments arranged by size: 

                1682    1373     356
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1516    3411 
   Size:       1516    1895
 
EaeI y'GGCC_r

Cuts at:      0       2     509    1000    1491    1821    2351    2435    2803 
   Size:          2     507     491     491     330     530      84     368

Cuts at:   2803    2861    3411 
   Size:         58     550

  Fragments arranged by size: 

                 550     530     507     491     491     368     330      84
                  58       2
 
EarI CTCTTCn'nnn_

Cuts at:      0      85     157     242     277     824    1040    1408    2257 
   Size:         85      72      85      35     547     216     368     849

Cuts at:   2257    2458    3411 
   Size:        201     953

  Fragments arranged by size: 

                 953     849     547     368     216     201      85      85
                  72      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     284     313     481     869    1306    1389    1489    1708 
   Size:        284      29     168     388     437      83     100     219

Cuts at:   1708    1717    1774    1820    1918    2080    2353    2668    2767 
   Size:          9      57      46      98     162     273     315      99

Cuts at:   2767    2974    3411 
   Size:        207     437

  Fragments arranged by size: 

                 437     437     388     315     284     273     219     207
                 168     162     100      99      98      83      57      46
                  29       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     892    3411 
   Size:        892    2519
 
EcoO109I rG'GnC_Cy

Cuts at:      0      32     228     394     504     644     645    1167    2181 
   Size:         32     196     166     110     140       1     522    1014

Cuts at:   2181    2662    3330    3379    3411 
   Size:        481     668      49      32

  Fragments arranged by size: 

                1014     668     522     481     196     166     140     110
                  49      32      32       1
 
EcoRI G'AATT_C

Cuts at:      0    1984    3411 
   Size:       1984    1427
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1771    3411 
   Size:       1771    1640
 
FspI TGC'GCA

Cuts at:      0     127     892    3411 
   Size:        127     765    2519

  Fragments arranged by size: 

                2519     765     127
 
HaeII r_GCGC'y

Cuts at:      0     905     945    1041    3411 
   Size:        905      40      96    2370

  Fragments arranged by size: 

                2370     905      96      40
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     305     337    1092    1124    1406    1438    2384    2416 
   Size:        305      32     755      32     282      32     946      32

Cuts at:   2416    3411 
   Size:        995

  Fragments arranged by size: 

                 995     946     755     305     282      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0    1753    3411 
   Size:       1753    1658
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     506    2013    2564    3411 
   Size:        506    1507     551     847

  Fragments arranged by size: 

                1507     847     551     506
 
MscI TGG'CCA

Cuts at:      0     511    1002    1823    2353    2437    2805    3411 
   Size:        511     491     821     530      84     368     606

  Fragments arranged by size: 

                 821     606     530     511     491     368      84
 
MslI CAynn'nnrTG

Cuts at:      0     428     872    1396    1697    1768    2005    2711    3007 
   Size:        428     444     524     301      71     237     706     296

Cuts at:   3007    3088    3411 
   Size:         81     323

  Fragments arranged by size: 

                 706     524     444     428     323     301     296     237
                  81      71
 
MspA1I CmG'CkG

Cuts at:      0     604     714    1878    1944    2882    2968    3411 
   Size:        604     110    1164      66     938      86     443

  Fragments arranged by size: 

                1164     938     604     443     110      86      66
 
NcoI C'CATG_G

Cuts at:      0    1242    2000    3083    3411 
   Size:       1242     758    1083     328

  Fragments arranged by size: 

                1242    1083     758     328
 
NheI G'CTAG_C

Cuts at:      0    3153    3411 
   Size:       3153     258
 
NspI r_CATG'y

Cuts at:      0    2796    2923    3411 
   Size:       2796     127     488

  Fragments arranged by size: 

                2796     488     127
 
PasI CC'CwG_GG

Cuts at:      0    2565    3411 
   Size:       2565     846
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     231     369     459     507    2053    2139    3411 
   Size:        231     138      90      48    1546      86    1272

  Fragments arranged by size: 

                1546    1272     231     138      90      86      48
 
PfoI T'CCnGG_A

Cuts at:      0      37     458     515    1733    2305    3411 
   Size:         37     421      57    1218     572    1106

  Fragments arranged by size: 

                1218    1106     572     421      57      37
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     365     397    3411 
   Size:        365      32    3014

  Fragments arranged by size: 

                3014     365      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     504    1167    3411 
   Size:        504     663    2244

  Fragments arranged by size: 

                2244     663     504
 
PspOMI G'GGCC_C

Cuts at:      0     644    2180    3330    3378    3411 
   Size:        644    1536    1150      48      33

  Fragments arranged by size: 

                1536    1150     644      48      33
 
PstI C_TGCA'G

Cuts at:      0    1943    3411 
   Size:       1943    1468
 
PvuII CAG'CTG

Cuts at:      0     604     714    1944    2882    2968    3411 
   Size:        604     110    1230     938      86     443

  Fragments arranged by size: 

                1230     938     604     443     110      86
 
SapI GCTCTTCn'nnn_

Cuts at:      0      85     242     824    1040    1408    3411 
   Size:         85     157     582     216     368    2003

  Fragments arranged by size: 

                2003     582     368     216     157      85
 
ScaI AGT'ACT

Cuts at:      0    2962    3411 
   Size:       2962     449
 
SfcI C'TryA_G

Cuts at:      0     309    1551    1782    1939    2892    3411 
   Size:        309    1242     231     157     953     519

  Fragments arranged by size: 

                1242     953     519     309     231     157
 
SmaI CCC'GGG

Cuts at:      0    1969    2854    3411 
   Size:       1969     885     557

  Fragments arranged by size: 

                1969     885     557
 
StuI AGG'CCT

Cuts at:      0     269    1669    3411 
   Size:        269    1400    1742

  Fragments arranged by size: 

                1742    1400     269
 
StyI C'CwwG_G

Cuts at:      0      25     564    1242    2000    2544    3083    3411 
   Size:         25     539     678     758     544     539     328

  Fragments arranged by size: 

                 758     678     544     539     539     328      25
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     106     205    1006    1192    1488    1718    1888    3275 
   Size:        106      99     801     186     296     230     170    1387

Cuts at:   3275    3411 
   Size:        136

  Fragments arranged by size: 

                1387     801     296     230     186     170     136     106
                  99
 
TatI w'GTAC_w

Cuts at:      0    1070    2960    3132    3411 
   Size:       1070    1890     172     279

  Fragments arranged by size: 

                1890    1070     279     172
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     349     381     534     566    3332    3364    3411 
   Size:        349      32     153      32    2766      32      47

  Fragments arranged by size: 

                2766     349     153      47      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1705    1776    3411 
   Size:       1705      71    1635

  Fragments arranged by size: 

                1705    1635      71
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     577    2430    3090    3411 
   Size:        577    1853     660     321

  Fragments arranged by size: 

                1853     660     577     321
 
XmaI C'CCGG_G

Cuts at:      0    1967    2852    3411 
   Size:       1967     885     559

  Fragments arranged by size: 

                1967     885     559
 
XmnI GAAnn'nnTTC

Cuts at:      0    1231    3411 
   Size:       1231    2180
 

 Enzymes that do cut:

     AccI     AcuI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AvaI     BanI    BanII     BbsI     BcgI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
     BtsI   DraIII     DrdI     EaeI     EarI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I  HindIII     MmeI     MscI
     MslI   MspA1I     NcoI     NheI     NspI     PasI    PflMI     PfoI
     PpiI    PpuMI   PspOMI     PstI    PvuII     SapI     ScaI     SfcI
     SmaI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI    BbvCI    BciVI     BclI    BfrBI    BpuEI    BsaAI    BsiEI
    BsiWI    BspEI    BsrGI   BssHII    BstBI    BtgZI     ClaI    CspCI
     DraI     EagI     EciI  EcoICRI    EcoRV     FseI    FspAI   HincII
     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI     NdeI
   NgoMIV     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     XbaI
     XhoI     ZraI
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