Restriction map for pF1KSDB0057
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AflIII      
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        AspEI       AspI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbsI        BciVI       BclI        BfrI        BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrGI       
BssHII      Bst1107I    BstBI       BstZ17I     CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HincII      HindII      HindIII     HpaI        
KasI        KspI        MamI        MfeI        MluI        
MroI        MseI        MunI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        ScaI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
SspBI       SspI        SwaI        Tru9I       Tth111I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

Acc65I       1259  GGTACC               G^GTACC
AccI          523  GTCTAC               GT^[AC][GT]AC
ApaI         1269  GGGCCC               GGGCC^C
Asp700I       451  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1259  GGTACC               G^GTACC
BcgI         1644  GCAGAAACGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          493  CTAG                 C^TAG
BglII         440  AGATCT               A^GATCT
BsaI          461  GAGACC               GGTCTC(1/5)
BsgI          675  GTGCAG               GTGCAG(16/14)
BspHI        1937  TCATGA               T^CATGA
BsrDI        2089  GCAATG               GCAATG(2/0)
BstAPI       1458  GCAGAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        123  GGTGACC              G^GT[ACGT]ACC
BstPI         123  GGTGACC              G^GT[ACGT]ACC
BstXI        2084  CCATGGCAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoO65I       123  GGTGACC              G^GT[ACGT]ACC
KpnI         1259  GGTACC               GGTAC^C
MaeI          493  CTAG                 C^TAG
NaeI         1999  GCCGGC               GCC^GGC
NgoMIV       1999  GCCGGC               G^CCGGC
PshAI         516  GACTGGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1937  TCATGA               T^CATGA
SacI         1705  GAGCTC               GAGCT^C
SapI         1218  GAAGAGC              GCTCTTC(1/4)
SbfI         1380  CCTGCAGG             CCTGCA^GG
SexAI         416  ACCTGGT              A^CC[AT]GGT
SfiI           18  GGCCAGCGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I     1380  CCTGCAGG             CCTGCA^GG
TfiI          456  GATTC                G^A[AT]TC
XmnI          451  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcsI         1144  GAATTC               [GA]^AATT[CT]
             2173  GAATTC              
AocI          859  CCTAAGG              CC^T[ACGT]AGG
             1264  CCTGAGG             
ApoI         1144  GAATTC               [GA]^AATT[CT]
             2173  GAATTC              
BbvCI          57  CCTCAGC              CCTCAGC(-5/-2)
             2299  CCTCAGC             
BsmI          363  GAATGC               GAATGC(1/-1)
              996  GAATGC              
BsrFI        1943  ACCGGC               [GA]^CCGG[CT]
             1999  GCCGGC              
Bsu36I        859  CCTAAGG              CC^T[ACGT]AGG
             1264  CCTGAGG             
Cfr10I       1943  ACCGGC               [GA]^CCGG[CT]
             1999  GCCGGC              
Eco81I        859  CCTAAGG              CC^T[ACGT]AGG
             1264  CCTGAGG             
EcoNI        1728  CCTGCAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1845  CCTGGAGGAGG         
EcoRI        1144  GAATTC               G^AATTC
             2173  GAATTC              
NspI          576  GCATGT               [GA]CATG^[CT]
             2325  ACATGC              
PflMI        1983  CCAGCCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2279  CCAGATCCTGG         
PvuII        1177  CAGCTG               CAG^CTG
             2156  CAGCTG              
TaqI          459  TCGA                 T^CGA
             1901  TCGA                
TthHB8I       459  TCGA                 T^CGA
             1901  TCGA                
Van91I       1983  CCAGCCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2279  CCAGATCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  103 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbvI        BcnI        
BglI        BmyI        BpmI        BsaHI       BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrI        BssKI       BssSI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       PleI        PpuMI       PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        SmlI        StuI        StyI        
TseI        Tsp45I      Tsp509I     TspRI       XcmI        
XhoII       XmaCI       XmaI        
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