Restriction map for pF1KSDB0057
 (Linear) (Six-base) MAPSORT of: KIBB0057.seq  Check: 8870  from: 1  to: 2379



KIBB0057.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:19  ..

AccI GT'mk_AC

Cuts at:      0     524    2379 
   Size:        524    1855
 
Acc65I G'GTAC_C

Cuts at:      0    1259    2379 
   Size:       1259    1120
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1189    1642    2379 
   Size:       1189     453     737

  Fragments arranged by size: 

                1189     737     453
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1652    2379 
   Size:       1652     727
 
AlwNI CAG_nnn'CTG

Cuts at:      0      96     417    1464    1845    1989    2285    2379 
   Size:         96     321    1047     381     144     296      94

  Fragments arranged by size: 

                1047     381     321     296     144      96      94
 
ApaI G_GGCC'C

Cuts at:      0    1273    2379 
   Size:       1273    1106
 
ApoI r'AATT_y

Cuts at:      0    1144    2173    2379 
   Size:       1144    1029     206

  Fragments arranged by size: 

                1144    1029     206
 
AvaI C'yCGr_G

Cuts at:      0     150     340     511     602     804    1106    2075    2379 
   Size:        150     190     171      91     202     302     969     304

  Fragments arranged by size: 

                 969     304     302     202     190     171     150      91
            
 
BanI G'GyrC_C

Cuts at:      0      47    1259    1714    1992    2288    2379 
   Size:         47    1212     455     278     296      91

  Fragments arranged by size: 

                1212     455     296     278      91      47
 
BanII G_rGCy'C

Cuts at:      0     341    1273    1709    2379 
   Size:        341     932     436     670

  Fragments arranged by size: 

                 932     670     436     341
 
BbvCI CC'TCA_GC

Cuts at:      0      58    2300    2379 
   Size:         58    2242      79

  Fragments arranged by size: 

                2242      79      58
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1633    1667    2379 
   Size:       1633      34     712

  Fragments arranged by size: 

                1633     712      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      25     296    1291    2335    2379 
   Size:         25     271     995    1044      44

  Fragments arranged by size: 

                1044     995     271      44      25
 
BglII A'GATC_T

Cuts at:      0     440    2379 
   Size:        440    1939
 
Bme1580I G_kGCm'C

Cuts at:      0     301    1273    1717    2379 
   Size:        301     972     444     662

  Fragments arranged by size: 

                 972     662     444     301
 
BmgBI CAC'GTC

Cuts at:      0     108    2261    2379 
   Size:        108    2153     118

  Fragments arranged by size: 

                2153     118     108
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     124    1033    1267    2379 
   Size:        124     909     234    1112

  Fragments arranged by size: 

                1112     909     234     124
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     484     654    1074    2379 
   Size:        484     170     420    1305

  Fragments arranged by size: 

                1305     484     420     170
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     961    1201    1366    1423    1489    1510    1867 
   Size:        241     720     240     165      57      66      21     357

Cuts at:   1867    2138    2155    2379 
   Size:        271      17     224

  Fragments arranged by size: 

                 720     357     271     241     240     224     165      66
                  57      21      17
 
Bpu10I CC'TnA_GC

Cuts at:      0      58    2300    2379 
   Size:         58    2242      79

  Fragments arranged by size: 

                2242      79      58
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      16    1747    1864    1870    2379 
   Size:         16    1731     117       6     509

  Fragments arranged by size: 

                1731     509     117      16       6
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     455    2379 
   Size:        455    1924
 
BsaHI Gr'CG_yC

Cuts at:      0     182     521    2003    2379 
   Size:        182     339    1482     376

  Fragments arranged by size: 

                1482     376     339     182
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     454     484     526     556     940     970    1957    1987 
   Size:        454      30      42      30     384      30     987      30

Cuts at:   1987    2379 
   Size:        392

  Fragments arranged by size: 

                 987     454     392     384      42      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      85     531    1168    1315    1420    1423    1465    1486 
   Size:         85     446     637     147     105       3      42      21

Cuts at:   1486    1552    1708    1864    1936    1978    1981    2379 
   Size:         66     156     156      72      42       3     398

  Fragments arranged by size: 

                 637     446     398     156     156     147     105      85
                  72      66      42      42      21       3       3
 
BseYI C'CCAG_C

Cuts at:      0    1175    1197    1334    2379 
   Size:       1175      22     137    1045

  Fragments arranged by size: 

                1175    1045     137      22
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     696    2379 
   Size:        696    1683
 
BsiHKAI G_wGCw'C

Cuts at:      0     257     377     937    1709    2379 
   Size:        257     120     560     772     670

  Fragments arranged by size: 

                 772     670     560     257     120
 
BsmI GAATG_Cn'

Cuts at:      0     369    1002    2379 
   Size:        369     633    1377

  Fragments arranged by size: 

                1377     633     369
 
Bsp1286I G_dGCh'C

Cuts at:      0     257     301     341     377     937    1273    1709    1717 
   Size:        257      44      40      36     560     336     436       8

Cuts at:   1717    2379 
   Size:        662

  Fragments arranged by size: 

                 662     560     436     336     257      44      40      36
                   8
 
BspHI T'CATG_A

Cuts at:      0    1937    2379 
   Size:       1937     442
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     473    1388    1505    2379 
   Size:        473     915     117     874

  Fragments arranged by size: 

                 915     874     473     117
 
BsrBI CCG'CTC

Cuts at:      0     398     644    1398    1659    2379 
   Size:        398     246     754     261     720

  Fragments arranged by size: 

                 754     720     398     261     246
 
BsrDI GCAATG_nn'

Cuts at:      0    2096    2379 
   Size:       2096     283
 
BsrFI r'CCGG_y

Cuts at:      0    1943    1999    2379 
   Size:       1943      56     380

  Fragments arranged by size: 

                1943     380      56
 
BssSI C'ACGA_G

Cuts at:      0      85     730    2201    2379 
   Size:         85     645    1471     178

  Fragments arranged by size: 

                1471     645     178      85
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1464    2379 
   Size:       1464     915
 
BstEII G'GTnAC_C

Cuts at:      0     123    2379 
   Size:        123    2256
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2091    2379 
   Size:       2091     288
 
BstYI r'GATC_y

Cuts at:      0      92     440    1802    2281    2379 
   Size:         92     348    1362     479      98

  Fragments arranged by size: 

                1362     479     348      98      92
 
Bsu36I CC'TnA_GG

Cuts at:      0     860    1265    2379 
   Size:        860     405    1114

  Fragments arranged by size: 

                1114     860     405
 
BtgI C'CryG_G

Cuts at:      0     101    1547    2084    2379 
   Size:        101    1446     537     295

  Fragments arranged by size: 

                1446     537     295     101
 
BtsI GCAGTG_nn'

Cuts at:      0     837    1132    1978    2048    2379 
   Size:        837     295     846      70     331

  Fragments arranged by size: 

                 846     837     331     295      70
 
EaeI y'GGCC_r

Cuts at:      0       2     288     696    1532    2029    2379 
   Size:          2     286     408     836     497     350

  Fragments arranged by size: 

                 836     497     408     350     286       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     446    1180    1213    1292    1618    2379 
   Size:        446     734      33      79     326     761

  Fragments arranged by size: 

                 761     734     446     326      79      33
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1236    1813    2379 
   Size:       1236     577     566

  Fragments arranged by size: 

                1236     577     566
 
EcoICRI GAG'CTC

Cuts at:      0    1707    2379 
   Size:       1707     672
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     961    1189    1201    1366    1423    1489    1510 
   Size:        241     720     228      12     165      57      66      21

Cuts at:   1510    1642    1867    2138    2155    2379 
   Size:        132     225     271      17     224

  Fragments arranged by size: 

                 720     271     241     228     225     224     165     132
                  66      57      21      17      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1732    1849    2379 
   Size:       1732     117     530

  Fragments arranged by size: 

                1732     530     117
 
EcoO109I rG'GnC_Cy

Cuts at:      0      27     236     326     534     769    1119    1269    1494 
   Size:         27     209      90     208     235     350     150     225

Cuts at:   1494    1842    2096    2379 
   Size:        348     254     283

  Fragments arranged by size: 

                 350     348     283     254     235     225     209     208
                 150      90      27
 
EcoRI G'AATT_C

Cuts at:      0    1144    2173    2379 
   Size:       1144    1029     206

  Fragments arranged by size: 

                1144    1029     206
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     184     216     454     486     526     558    2379 
   Size:        184      32     238      32      40      32    1821

  Fragments arranged by size: 

                1821     238     184      40      32      32      32
 
KpnI G_GTAC'C

Cuts at:      0    1263    2379 
   Size:       1263    1116
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1595    2120    2379 
   Size:       1595     525     259

  Fragments arranged by size: 

                1595     525     259
 
MscI TGG'CCA

Cuts at:      0       4     290     698    2379 
   Size:          4     286     408    1681

  Fragments arranged by size: 

                1681     408     286       4
 
MslI CAynn'nnrTG

Cuts at:      0     106     622     626     667     712    2089    2379 
   Size:        106     516       4      41      45    1377     290

  Fragments arranged by size: 

                1377     516     290     106      45      41       4
 
MspA1I CmG'CkG

Cuts at:      0     781    1179    2158    2304    2379 
   Size:        781     398     979     146      75

  Fragments arranged by size: 

                 979     781     398     146      75
 
NaeI GCC'GGC

Cuts at:      0    2001    2379 
   Size:       2001     378
 
NcoI C'CATG_G

Cuts at:      0     101    1547    2084    2379 
   Size:        101    1446     537     295

  Fragments arranged by size: 

                1446     537     295     101
 
NgoMIV G'CCGG_C

Cuts at:      0    1999    2379 
   Size:       1999     380
 
NspI r_CATG'y

Cuts at:      0     580    2329    2379 
   Size:        580    1749      50

  Fragments arranged by size: 

                1749     580      50
 
PasI CC'CwG_GG

Cuts at:      0     897     987    2379 
   Size:        897      90    1392

  Fragments arranged by size: 

                1392     897      90
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1989    2285    2379 
   Size:       1989     296      94

  Fragments arranged by size: 

                1989     296      94
 
PfoI T'CCnGG_A

Cuts at:      0     977    1343    1424    1685    1805    1952    2379 
   Size:        977     366      81     261     120     147     427

  Fragments arranged by size: 

                 977     427     366     261     147     120      81
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1868    1900    2379 
   Size:       1868      32     479

  Fragments arranged by size: 

                1868     479      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     326     534    1119    1494    1842    2096    2379 
   Size:        326     208     585     375     348     254     283

  Fragments arranged by size: 

                 585     375     348     326     283     254     208
 
PshAI GACnn'nnGTC

Cuts at:      0     520    2379 
   Size:        520    1859
 
PspOMI G'GGCC_C

Cuts at:      0    1269    2379 
   Size:       1269    1110
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     397     429    2379 
   Size:        397      32    1950

  Fragments arranged by size: 

                1950     397      32
 
PstI C_TGCA'G

Cuts at:      0    1385    1733    1787    2043    2379 
   Size:       1385     348      54     256     336

  Fragments arranged by size: 

                1385     348     336     256      54
 
PvuII CAG'CTG

Cuts at:      0    1179    2158    2379 
   Size:       1179     979     221

  Fragments arranged by size: 

                1179     979     221
 
SacI G_AGCT'C

Cuts at:      0    1709    2379 
   Size:       1709     670
 
SanDI GG'GwC_CC

Cuts at:      0    2096    2379 
   Size:       2096     283
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1213    2379 
   Size:       1213    1166
 
SbfI CC_TGCA'GG

Cuts at:      0    1385    2379 
   Size:       1385     994
 
SexAI A'CCwGG_T

Cuts at:      0     416    2379 
   Size:        416    1963
 
SfcI C'TryA_G

Cuts at:      0    1381    1729    1783    2039    2379 
   Size:       1381     348      54     256     340

  Fragments arranged by size: 

                1381     348     340     256      54
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      25    2379 
   Size:         25    2354
 
SmaI CCC'GGG

Cuts at:      0     152     604    1108    2379 
   Size:        152     452     504    1271

  Fragments arranged by size: 

                1271     504     452     152
 
SmlI C'TyrA_G

Cuts at:      0      31    1762    1879    1885    2379 
   Size:         31    1731     117       6     494

  Fragments arranged by size: 

                1731     494     117      31       6
 
StuI AGG'CCT

Cuts at:      0     858    1285    2058    2379 
   Size:        858     427     773     321

  Fragments arranged by size: 

                 858     773     427     321
 
StyI C'CwwG_G

Cuts at:      0      14     101     473    1124    1355    1547    2084    2379 
   Size:         14      87     372     651     231     192     537     295

  Fragments arranged by size: 

                 651     537     372     295     231     192      87      14
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     321     400     634     952    1278    2379 
   Size:        321      79     234     318     326    1101

  Fragments arranged by size: 

                1101     326     321     318     234      79
 
TatI w'GTAC_w

Cuts at:      0    1538    2379 
   Size:       1538     841
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     927     959    1957    1989    2379 
   Size:        927      32     998      32     390

  Fragments arranged by size: 

                 998     927     390      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     265     298    1008    2379 
   Size:        265      33     710    1371

  Fragments arranged by size: 

                1371     710     265      33
 
XmaI C'CCGG_G

Cuts at:      0     150     602    1106    2379 
   Size:        150     452     504    1273

  Fragments arranged by size: 

                1273     504     452     150
 
XmnI GAAnn'nnTTC

Cuts at:      0     455    2379 
   Size:        455    1924
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AlfI    AlwNI     ApaI     ApoI     AvaI
     BanI    BanII    BbvCI     BcgI     BglI    BglII Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI   BstEII    BstXI    BstYI
   Bsu36I     BtgI     BtsI     EaeI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    Hin4I     KpnI     MmeI     MscI     MslI
   MspA1I     NaeI     NcoI   NgoMIV     NspI     PasI    PflMI     PfoI
     PpiI    PpuMI    PshAI   PspOMI     PsrI     PstI    PvuII     SacI
    SanDI     SapI     SbfI    SexAI     SfcI     SfiI     SmaI     SmlI
     StuI     StyI    TaqII     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BbsI    BciVI     BclI    BfrBI     BlpI     BmtI
    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI    BsmBI    BspEI    BsrGI
   BssHII    BstBI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     MfeI     MluI     NarI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI
     PsiI    PspXI     PvuI    RsrII    SacII     SalI     ScaI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
  Tth111I     XbaI     XhoI     ZraI
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