Restriction map for pF1KSDB0037
 (Linear) (Six-base) MAPSORT of: KIBB0037.seq  Check: 4957  from: 1  to: 1413



KIBB0037.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AatII G_ACGT'C

Cuts at:      0     653    1413 
   Size:        653     760
 
AccI GT'mk_AC

Cuts at:      0    1289    1413 
   Size:       1289     124
 
Acc65I G'GTAC_C

Cuts at:      0    1146    1413 
   Size:       1146     267
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     604     775    1413 
   Size:        604     171     638

  Fragments arranged by size: 

                 638     604     171
 
AleI CACnn'nnGTG

Cuts at:      0     968    1413 
   Size:        968     445
 
ApoI r'AATT_y

Cuts at:      0     529     549    1376    1413 
   Size:        529      20     827      37

  Fragments arranged by size: 

                 827     529      37      20
 
AvaI C'yCGr_G

Cuts at:      0     734    1413 
   Size:        734     679
 
BamHI G'GATC_C

Cuts at:      0    1238    1413 
   Size:       1238     175
 
BanI G'GyrC_C

Cuts at:      0     115    1146    1413 
   Size:        115    1031     267

  Fragments arranged by size: 

                1031     267     115
 
BanII G_rGCy'C

Cuts at:      0    1222    1413 
   Size:       1222     191
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     645    1130    1413 
   Size:        645     485     283

  Fragments arranged by size: 

                 645     485     283
 
BbvCI CC'TCA_GC

Cuts at:      0    1002    1413 
   Size:       1002     411
 
BglII A'GATC_T

Cuts at:      0     809    1413 
   Size:        809     604
 
Bme1580I G_kGCm'C

Cuts at:      0     836    1413 
   Size:        836     577
 
BmgBI CAC'GTC

Cuts at:      0     660    1039    1413 
   Size:        660     379     374

  Fragments arranged by size: 

                 660     379     374
 
Bpu10I CC'TnA_GC

Cuts at:      0    1002    1413 
   Size:       1002     411
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     406     640    1413 
   Size:        406     234     773

  Fragments arranged by size: 

                 773     406     234
 
BsaBI GATnn'nnATC

Cuts at:      0      16     269    1013    1413 
   Size:         16     253     744     400

  Fragments arranged by size: 

                 744     400     253      16
 
BsaHI Gr'CG_yC

Cuts at:      0     650    1413 
   Size:        650     763
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     955    1413 
   Size:        955     458
 
BseYI C'CCAG_C

Cuts at:      0     202    1413 
   Size:        202    1211
 
BsmI GAATG_Cn'

Cuts at:      0     497    1413 
   Size:        497     916
 
Bsp1286I G_dGCh'C

Cuts at:      0     836    1222    1413 
   Size:        836     386     191

  Fragments arranged by size: 

                 836     386     191
 
BsrDI GCAATG_nn'

Cuts at:      0      79     746    1413 
   Size:         79     667     667

  Fragments arranged by size: 

                 667     667      79
 
BsrFI r'CCGG_y

Cuts at:      0      52    1413 
   Size:         52    1361
 
BstYI r'GATC_y

Cuts at:      0     809    1238    1272    1413 
   Size:        809     429      34     141

  Fragments arranged by size: 

                 809     429     141      34
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1000    1413 
   Size:       1000     413
 
DraI TTT'AAA

Cuts at:      0     371    1413 
   Size:        371    1042
 
EaeI y'GGCC_r

Cuts at:      0       2      50    1413 
   Size:          2      48    1363

  Fragments arranged by size: 

                1363      48       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     323     905    1413 
   Size:        323     582     508

  Fragments arranged by size: 

                 582     508     323
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     695    1413 
   Size:        695     718
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     604     775    1413 
   Size:        604     171     638

  Fragments arranged by size: 

                 638     604     171
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     152    1413 
   Size:        152    1261
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1012    1413 
   Size:       1012     401
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     888     920    1307    1330    1339    1362    1413 
   Size:        888      32     387      23       9      23      51

  Fragments arranged by size: 

                 888     387      51      32      23      23       9
 
KpnI G_GTAC'C

Cuts at:      0    1150    1413 
   Size:       1150     263
 
MfeI C'AATT_G

Cuts at:      0     557    1413 
   Size:        557     856
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     314    1413 
   Size:        314    1099
 
MscI TGG'CCA

Cuts at:      0       4    1413 
   Size:          4    1409
 
MslI CAynn'nnrTG

Cuts at:      0     421     968    1413 
   Size:        421     547     445

  Fragments arranged by size: 

                 547     445     421
 
NaeI GCC'GGC

Cuts at:      0      54    1413 
   Size:         54    1359
 
NdeI CA'TA_TG

Cuts at:      0     822    1413 
   Size:        822     591
 
NgoMIV G'CCGG_C

Cuts at:      0      52    1413 
   Size:         52    1361
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     663    1413 
   Size:        663     750
 
ScaI AGT'ACT

Cuts at:      0     796    1413 
   Size:        796     617
 
SfcI C'TryA_G

Cuts at:      0    1286    1413 
   Size:       1286     127
 
TatI w'GTAC_w

Cuts at:      0     794    1413 
   Size:        794     619
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1215    1247    1413 
   Size:       1215      32     166

  Fragments arranged by size: 

                1215     166      32
 
XbaI T'CTAG_A

Cuts at:      0     690    1413 
   Size:        690     723
 
ZraI GAC'GTC

Cuts at:      0     651    1413 
   Size:        651     762
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI     AleI     ApoI     AvaI    BamHI
     BanI    BanII     BbsI    BbvCI    BglII Bme1580I    BmgBI   Bpu10I
     BsaI    BsaBI    BsaHI    BseRI    BseYI     BsmI Bsp1286I    BsrDI
    BsrFI    BstYI    BtgZI     DraI     EaeI     EarI     EciI  Eco57MI
    EcoNI     FalI    Hin4I     KpnI     MfeI     MmeI     MscI     MslI
     NaeI     NdeI   NgoMIV    PflMI     ScaI     SfcI     TatI     TstI
     XbaI     ZraI

 Enzymes that do not cut: 

     AarI     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AlfI
     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
     BmrI     BmtI     BplI     BpmI    BpuEI    BsaAI    BsaWI    BsaXI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI   DraIII     DrdI
     EagI  EcoICRI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     MluI   MspA1I     NarI
     NcoI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII  Tth111I     XcmI     XhoI
     XmaI     XmnI
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