Restriction map for
pF1KSDB0036
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI AocI Aor51HI
AscI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BcgI BclI BcnI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmFI
BsoBI BspDI BspEI BspMI BsrBI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
ClaI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FauI FbaI
FseI FspI Hin1I KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 360 GTCTAC GT^[AC][GT]AC
AcsI 1814 GAATTC [GA]^AATT[CT]
AflII 315 CTTAAG C^TTAAG
Alw44I 141 GTGCAC G^TGCAC
AlwI 1702 GGATC GGATC(4/5)
AlwNI 10 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 106 GGGCCC GGGCC^C
ApaLI 141 GTGCAC G^TGCAC
ApoI 1814 GAATTC [GA]^AATT[CT]
AseI 646 ATTAAT AT^TAAT
AsnI 646 ATTAAT AT^TAAT
AvaII 21 GGACC G^G[AT]CC
BanI 1758 GGCACC G^G[CT][GA]CC
BanII 106 GGGCCC G[GA]GC[CT]^C
BbvCI 1088 CCTCAGC CCTCAGC(-5/-2)
BfrI 315 CTTAAG C^TTAAG
BpmI 1400 CTCCAG CTGGAG(16/14)
BsaI 1884 GAGACC GGTCTC(1/5)
BsgI 1109 CTGCAC GTGCAG(16/14)
BsmAI 1884 GAGAC GTCTC(1/5)
BsmI 1743 GAATGC GAATGC(1/-1)
Bsp1407I 1212 TGTACA T^GTACA
BspLU11I 356 ACATGT A^CATGT
BsrDI 554 GCAATG GCAATG(2/0)
BsrFI 52 ACCGGC [GA]^CCGG[CT]
BsrGI 1212 TGTACA T^GTACA
BssSI 94 CACGAG CACGAG(-5/-1)
BstXI 1613 CCAACCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1702 GGATCT [GA]^GATC[CT]
CfoI 1023 GCGC GCG^C
Cfr10I 52 ACCGGC [GA]^CCGG[CT]
CpoI 20 CGGACCG CG^G[AT]CCG
EaeI 6 CGGCCA [CT]^GGCC[GA]
EarI 444 GAAGAG CTCTTC(1/4)
EcoRI 1814 GAATTC G^AATTC
EcoT14I 851 CCTTGG C^C[AT][AT]GG
HaeII 1022 AGCGCC [GA]GCGC^[CT]
HgaI 1848 GACGC GACGC(5/10)
HhaI 1023 GCGC GCG^C
HinP1I 1023 GCGC G^CGC
HpaI 1692 GTTAAC GTT^AAC
Ksp632I 444 GAAGAG CTCTTC(1/4)
MaeIII 49 GTCAC ^GT[ACGT]AC
MflI 1702 GGATCT [GA]^GATC[CT]
MslI 435 CACAAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 356 ACATGT [GA]CATG^[CT]
PleI 1671 GAGTC GAGTC(4/5)
PshBI 646 ATTAAT AT^TAAT
PstI 1067 CTGCAG CTGCA^G
RsrII 20 CGGACCG CG^G[AT]CCG
SexAI 1677 ACCAGGT A^CC[AT]GGT
SmlI 315 CTTAAG C^T[CT][GA]AG
SspBI 1212 TGTACA T^GTACA
SspI 662 AATATT AAT^ATT
StyI 851 CCTTGG C^C[AT][AT]GG
SwaI 389 ATTTAAAT ATTT^AAAT
Tsp45I 49 GTCAC ^GT[GC]AC
XcmI 1729 CCAACAACCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1702 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 302 ACACGT A^C[GA][CT]GT
356 ACATGT
AspHI 141 GTGCAC G[AT]GC[AT]^C
1916 GAGCAC
BciVI 64 GTATCC GTATCC(6/5)
1081 GTATCC
BsaJI 851 CCTTGG C^C[ACGT][ACGT]GG
923 CCCTGG
BsiHKAI 141 GTGCAC G[AT]GC[AT]^C
1916 GAGCAC
BspHI 1371 TCATGA T^CATGA
1880 TCATGA
DraI 390 TTTAAA TTT^AAA
1011 TTTAAA
DraII 105 GGGGCCC [GA]G^G[ACGT]CC[CT]
806 AGGCCCC
EcoNI 798 CCTTTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1906 CCTGTATCAGG
EcoO109I 105 GGGGCCC [GA]G^G[ACGT]CC[CT]
806 AGGCCCC
HapII 5 CCGG C^CGG
53 CCGG
HindIII 1007 AAGCTT A^AGCTT
1281 AAGCTT
HpaII 5 CCGG C^CGG
53 CCGG
MaeII 304 ACGT A^CGT
999 ACGT
MspA1I 881 CAGCTG C[AC]G^C[GT]G
1424 CAGCTG
MspI 5 CCGG C^CGG
53 CCGG
PvuII 881 CAGCTG CAG^CTG
1424 CAGCTG
RcaI 1371 TCATGA T^CATGA
1880 TCATGA
SfcI 878 CTACAG C^T[GA][CT]AG
1067 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI BbsI BbvI
BfaI BmyI BpuAI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HincII HindII
HinfI HphI ItaI MaeI MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI Tru9I
TseI Tsp509I TspRI