Restriction map for pF1KB8806
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       AccI        AccII       AclI        AcyI        
AflII       AflIII      AgeI        AhdI        AocI        
Aor51HI     AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BcnI        BfaI        BfrI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BseRI       BsgI        
BsiEI       BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       BspDI       BspLU11I    BsrBI       
BsrDI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRV       EcoT22I     FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HpaI        KasI        KspI        
MaeI        MaeII       MamI        MfeI        MluI        
MspA1I      MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
SacI        SacII       SalI        SbfI        ScaI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I        392  GGTACC               G^GTACC
AccIII        923  TCCGGA               T^CCGGA
AcsI          529  GAATTC               [GA]^AATT[CT]
Alw44I        964  GTGCAC               G^TGCAC
AlwNI         595  CAGGCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         964  GTGCAC               G^TGCAC
ApoI          529  GAATTC               [GA]^AATT[CT]
Asp718I       392  GGTACC               G^GTACC
AspHI         964  GTGCAC               G[AT]GC[AT]^C
BanI          392  GGTACC               G^G[CT][GA]CC
BglII         359  AGATCT               A^GATCT
BpmI         1006  CTGGAG               CTGGAG(16/14)
BsaWI         923  TCCGGA               [AT]^CCGG[AT]
BseAI         923  TCCGGA               T^CCGGA
BsiHKAI       964  GTGCAC               G[AT]GC[AT]^C
Bsp1407I      350  TGTACA               T^GTACA
BspEI         923  TCCGGA               T^CCGGA
BsrGI         350  TGTACA               T^GTACA
Cac8I         426  GCGGGC               GC[ACGT]^[ACGT]GC
CpoI          433  CGGACCG              CG^G[AT]CCG
DdeI          679  CTAAG                C^T[ACGT]AG
DraIII        409  CACGCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          995  CCGTGG               C^C[GA][CT]GG
EarI          244  CTCTTC               CTCTTC(1/4)
EcoRI         529  GAATTC               G^AATTC
EcoT14I       125  CCTTGG               C^C[AT][AT]GG
FauI          426  GCGGG                CCCGC(4/6)
HapII         924  CCGG                 C^CGG
HincII        886  GTCAAC               GT[CT]^[GA]AC
HindII        886  GTCAAC               GT[CT]^[GA]AC
HinfI         672  GATTC                G^A[ACGT]TC
HpaII         924  CCGG                 C^CGG
KpnI          392  GGTACC               GGTAC^C
Ksp632I       244  CTCTTC               CTCTTC(1/4)
MroI          923  TCCGGA               T^CCGGA
MseI          974  TTAA                 T^TAA
MspI          924  CCGG                 C^CGG
PstI          546  CTGCAG               CTGCA^G
RsrII         433  CGGACCG              CG^G[AT]CCG
SapI          243  GCTCTTC              GCTCTTC(1/4)
SexAI         968  ACCTGGT              A^CC[AT]GGT
SfcI          546  CTGCAG               C^T[GA][CT]AG
SfiI           90  GGCCTTTGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI         350  TGTACA               T^GTACA
StyI          125  CCTTGG               C^C[AT][AT]GG
TaqI          272  TCGA                 T^CGA
TfiI          672  GATTC                G^A[AT]TC
Tru9I         974  TTAA                 T^TAA
TthHB8I       272  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AciI          426  GCGG                 CCGC(-3/-1)
              936  GCGG                
AlwI          321  GATCC                GGATC(4/5)
              269  GGATC               
ApaI           98  GGGCCC               GGGCC^C
              473  GGGCCC              
BanII          98  GGGCCC               G[GA]GC[CT]^C
              473  GGGCCC              
BbvI          548  GCAGC                GCAGC(8/12)
              108  GCTGC               
BclI          644  TGATCA               T^GATCA
              734  TGATCA              
BspHI         641  TCATGA               T^CATGA
              731  TCATGA              
BsrI          293  ACTGG                ACTGG(1/-1)
              892  CCAGT               
BstYI         320  AGATCC               [GA]^GATC[CT]
              359  AGATCT              
FbaI          644  TGATCA               T^GATCA
              734  TGATCA              
MaeIII        439  GTCAC                ^GT[ACGT]AC
              853  GTGAC               
MboII         696  GAAGA                GAAGA(8/7)
              245  TCTTC               
MflI          320  AGATCC               [GA]^GATC[CT]
              359  AGATCT              
RcaI          641  TCATGA               T^CATGA
              731  TCATGA              
TseI          108  GCTGC                G^C[AT]GC
              548  GCAGC               
Tsp45I        439  GTCAC                ^GT[GC]AC
              853  GTGAC               
Tsp509I       530  AATT                 ^AATT
              766  AATT                
TspRI         877  CACTG                CA[GC]TG[ACGT][ACGT]^
              893  CAGTG               
XhoII         320  AGATCC               [GA]^GATC[CT]
              359  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AfaI        AluI        AvaII       BalI        BglI        
BmyI        BsaJI       BsiYI       BslI        Bsp1286I    
BspMI       BssKI       BstF5I      BstNI       Cfr13I      
DpnII       DraII       EaeI        EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HphI        ItaI        
MboI        MluNI       MnlI        MscI        MslI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
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