Restriction map for pF1KSDA1994
##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AatII       AclI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        ApaI        ApaLI       
AscI        AseI        AsnI        AspEI       AspI        
AvaI        AviII       AvrII       BamHI       BbeI        
BbrPI       BcgI        BfrI        BglI        BlnI        
BsaAI       BsaBI       BsiWI       BsmBI       BsoBI       
BspDI       BspHI       BspLU11I    BsrBI       BssHII      
BssSI       BstBI       Bsu36I      ClaI        CpoI        
DraIII      DrdI        Eam1105I    Eco81I      EcoRI       
EcoRV       EcoT22I     FseI        FspI        HindIII     
KasI        MamI        MluI        MslI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SalI        SapI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SphI        StuI        SwaI        Tth111I     
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

Acc65I        133  GGTACC               G^GTACC
AccI         1601  GTATAC               GT^[AC][GT]AC
AccII          27  CGCG                 CG^CG
AccIII       2005  TCCGGA               T^CCGGA
AcyI          139  GGCGTC               G[GA]^CG[CT]C
Aor51HI        52  AGCGCT               AGC^GCT
Asp718I       133  GGTACC               G^GTACC
BbvCI         589  CCTCAGC              CCTCAGC(-5/-2)
BclI          629  TGATCA               T^GATCA
BcnI           72  CCCGG                CC^[GC]GG
BsaHI         139  GGCGTC               G[GA]^CG[CT]C
BsaI          523  GAGACC               GGTCTC(1/5)
BsaWI        2005  TCCGGA               [AT]^CCGG[AT]
BseAI        2005  TCCGGA               T^CCGGA
BsiEI          29  CGGCCG               CG[GA][CT]^CG
Bsp1407I     1968  TGTACA               T^GTACA
BspEI        2005  TCCGGA               T^CCGGA
BsrFI         136  ACCGGC               [GA]^CCGG[CT]
BsrGI        1968  TGTACA               T^GTACA
Bst1107I     1601  GTATAC               GTA^TAC
BstEII        703  GGTCACC              G^GT[ACGT]ACC
BstPI         703  GGTCACC              G^GT[ACGT]ACC
BstUI          27  CGCG                 CG^CG
BstXI        2130  CCAGCCTGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1601  GTATAC               GTA^TAC
Cfr10I        136  ACCGGC               [GA]^CCGG[CT]
DraI         1255  TTTAAA               TTT^AAA
DraII        3011  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DsaI           26  CCGCGG               C^C[GA][CT]GG
EagI           29  CGGCCG               C^GGCCG
EclXI          29  CGGCCG               C^GGCCG
Eco47III       52  AGCGCT               AGC^GCT
Eco52I         29  CGGCCG               C^GGCCG
EcoNI         261  CCTTTCGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     3011  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       703  GGTCACC              G^GT[ACGT]ACC
FauI         3181  CCCGC                CCCGC(4/6)
FbaI          629  TGATCA               T^GATCA
HgaI          140  GCGTC                GACGC(5/10)
Hin1I         139  GGCGTC               G[GA]^CG[CT]C
KpnI          133  GGTACC               GGTAC^C
KspI           26  CCGCGG               CCGC^GG
MaeII         220  ACGT                 A^CGT
MroI         2005  TCCGGA               T^CCGGA
MvnI           27  CGCG                 CG^CG
NciI           72  CCCGG                CC^[GC]GG
NotI           28  GCGGCCGC             GC^GGCCGC
PflMI        1551  CCAACAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        3011  AGGTCCT              [GA]G^G[AT]CC[CT]
SacI         2910  GAGCTC               GAGCT^C
SacII          26  CCGCGG               CCGC^GG
SbfI         2113  CCTGCAGG             CCTGCA^GG
ScaI          889  AGTACT               AGT^ACT
Sse8387I     2113  CCTGCAGG             CCTGCA^GG
SspBI        1968  TGTACA               T^GTACA
Van91I       1551  CCAACAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          775  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AlwNI        2156  CAGTGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2840  CAGCCTCTG           
Asp700I      2435  GAATTGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2528  GAATGCCTTC          
BbsI         3081  GAAGAC               GAAGAC(2/6)
             1682  GTCTTC              
BciVI        1727  GTATCC               GTATCC(6/5)
             2072  GTATCC              
BlpI          271  GCTCAGC              GC^T[ACGT]AGC
             2059  GCTCAGC             
Bpu1102I      271  GCTCAGC              GC^T[ACGT]AGC
             2059  GCTCAGC             
BpuAI        3081  GAAGAC               GAAGAC(2/6)
             1682  GTCTTC              
BsmFI         225  GGGAC                GGGAC(10/14)
             2101  GTCCC               
BsmI          639  GAATGC               GAATGC(1/-1)
             2528  GAATGC              
CelII         271  GCTCAGC              GC^T[ACGT]AGC
             2059  GCTCAGC             
EarI          447  CTCTTC               CTCTTC(1/4)
              520  GAAGAG              
EcoT14I       996  CCTTGG               C^C[AT][AT]GG
             1536  CCAAGG              
HaeII          52  AGCGCT               [GA]GCGC^[CT]
              109  AGCGCC              
Ksp632I       447  CTCTTC               CTCTTC(1/4)
              520  GAAGAG              
MfeI         2359  CAATTG               C^AATTG
             2714  CAATTG              
MunI         2359  CAATTG               C^AATTG
             2714  CAATTG              
SspI         1024  AATATT               AAT^ATT
             2191  AATATT              
StyI          996  CCTTGG               C^C[AT][AT]GG
             1536  CCAAGG              
XmnI         2435  GAATTGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2528  GAATGCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BalI        BanI        
BanII       BbvI        BfaI        BglII       BmyI        
BpmI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       Bsp1286I    BspMI       
BsrDI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       EaeI        EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HincII      HindII      HinfI       HpaI        HpaII       
HphI        ItaI        MaeI        MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MspA1I      MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SpeI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII       
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